(Circular) (Six-base) MAPSORT of: pFN21AB9744.seq Check: 7844 from: 1 to: 6301 pFN21AB9744 6301 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3374 3374 Size: 6301 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3528 4111 278 Size: 53 83 186 936 1992 583 2468 Fragments arranged by size: 2468 1992 936 583 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2492 2582 2666 2852 2894 4364 5748 6016 Size: 860 90 84 186 42 1470 1384 268 Cuts at: 6016 1632 Size: 1917 Fragments arranged by size: 1917 1470 1384 860 268 186 90 84 42 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 5200 6177 1203 Size: 3997 977 1327 Fragments arranged by size: 3997 1327 977 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 3572 1166 Size: 2406 3895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6301 AlwNI CAG_nnn'CTG Cuts at: 2452 3055 3167 4044 5616 2452 Size: 603 112 877 1572 3137 Fragments arranged by size: 3137 1572 877 603 112 ApaI G_GGCC'C Cuts at: 2590 2984 2590 Size: 394 5907 ApaLI G'TGCA_C Cuts at: 2774 4358 5514 2774 Size: 1584 1156 3561 Fragments arranged by size: 3561 1584 1156 ApoI r'AATT_y Cuts at: 1493 3610 3836 3988 6097 1493 Size: 2117 226 152 2109 1697 Fragments arranged by size: 2117 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 2158 2406 2465 2861 3101 3626 1870 Size: 73 215 248 59 396 240 525 4545 Fragments arranged by size: 4545 525 396 248 240 215 73 59 BamHI G'GATC_C Cuts at: 2476 3631 3998 2476 Size: 1155 367 4779 Fragments arranged by size: 4779 1155 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2999 3010 3021 3262 3622 Size: 325 217 627 1212 11 11 241 360 Cuts at: 3622 5077 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1212 627 360 325 241 217 11 11 BanII G_rGCy'C Cuts at: 1391 2590 2768 2912 2984 3620 1391 Size: 1199 178 144 72 636 4072 Fragments arranged by size: 4072 1199 636 178 144 72 BbeI G_GCGC'C Cuts at: 3266 3266 Size: 6301 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2029 2542 2570 928 Size: 971 130 513 28 4659 Fragments arranged by size: 4659 971 513 130 28 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3484 3512 3518 3546 4492 4526 1903 Size: 34 1547 28 6 28 946 34 3678 Fragments arranged by size: 3678 1547 946 34 34 28 28 6 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 1548 Size: 6301 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2663 2748 3206 Size: 107 122 71 631 605 991 85 458 Cuts at: 3206 3356 4912 136 Size: 150 1556 1525 Fragments arranged by size: 1556 1525 991 631 605 458 150 122 107 85 71 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 2015 3056 3718 2015 Size: 1041 662 4598 Fragments arranged by size: 4598 1041 662 Bme1580I G_kGCm'C Cuts at: 1790 2590 2778 2984 3026 3179 4362 5518 1790 Size: 800 188 206 42 153 1183 1156 2573 Fragments arranged by size: 2573 1183 1156 800 206 188 153 42 BmgBI CAC'GTC Cuts at: 1318 2346 1318 Size: 1028 5273 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2420 2673 3189 Size: 416 53 83 399 24 1003 253 516 Cuts at: 3189 3309 4032 4990 442 Size: 120 723 958 1753 Fragments arranged by size: 1753 1003 958 723 516 416 399 253 120 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3248 1056 Size: 2192 4109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2898 3047 4961 1239 Size: 866 793 149 1914 2579 Fragments arranged by size: 2579 1914 866 793 149 Bpu10I CC'TnA_GC Cuts at: 2057 2057 Size: 6301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2273 4426 5291 5589 2273 Size: 2153 865 298 2985 Fragments arranged by size: 2985 2153 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2411 3116 4964 882 Size: 617 912 705 1848 2219 Fragments arranged by size: 2219 1848 912 705 617 BsaAI yAC'GTr Cuts at: 493 1204 3511 4092 493 Size: 711 2307 581 2702 Fragments arranged by size: 2702 2307 711 581 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3263 3525 4108 4490 Size: 53 83 186 936 1730 262 583 382 Cuts at: 4490 5125 275 Size: 635 1451 Fragments arranged by size: 1730 1451 936 635 583 382 262 186 83 53 BsaWI w'CCGG_w Cuts at: 2211 2484 2787 4734 5132 5406 5553 5964 2211 Size: 273 303 1947 398 274 147 411 2548 Fragments arranged by size: 2548 1947 411 398 303 274 273 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3404 3434 1178 Size: 30 165 30 2001 30 4045 Fragments arranged by size: 4045 2001 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2156 2323 2344 3404 1186 Size: 838 132 167 21 1060 4083 Fragments arranged by size: 4083 1060 838 167 132 21 BseYI C'CCAG_C Cuts at: 2130 2899 2939 3017 3033 3160 3434 5504 6000 Size: 769 40 78 16 127 274 2070 496 Cuts at: 6000 2130 Size: 2431 Fragments arranged by size: 2431 2070 769 496 274 127 78 40 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2225 1163 Size: 573 489 5239 Fragments arranged by size: 5239 573 489 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2743 3751 4512 5540 1540 Size: 275 186 742 1008 761 1028 2301 Fragments arranged by size: 2301 1028 1008 761 742 275 186 BsiHKAI G_wGCw'C Cuts at: 2768 2778 3510 4362 4447 5518 2768 Size: 10 732 852 85 1071 3551 Fragments arranged by size: 3551 1071 852 732 85 10 BsmI GAATG_Cn' Cuts at: 3812 3905 3812 Size: 93 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2590 2768 2778 2912 2984 3026 3179 Size: 399 800 178 10 134 72 42 153 Cuts at: 3179 3510 3620 4362 4447 5518 1391 Size: 331 110 742 85 1071 2174 Fragments arranged by size: 2174 1071 800 742 399 331 178 153 134 110 85 72 42 10 BspEI T'CCGG_A Cuts at: 2484 5964 2484 Size: 3480 2821 BspHI T'CATG_A Cuts at: 4190 5920 4190 Size: 1730 4571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2023 2811 3374 3656 844 Size: 1179 788 563 282 3489 Fragments arranged by size: 3489 1179 788 563 282 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4796 4970 65 Size: 1183 3548 174 1396 Fragments arranged by size: 3548 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2238 2274 4945 5132 1700 Size: 110 428 36 2671 187 2869 Fragments arranged by size: 2869 2671 428 187 110 36 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6301 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 4361 5373 4361 Size: 1012 5289 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstXI CCAn_nnnn'nTGG Cuts at: 2686 2824 3259 3574 2686 Size: 138 435 315 5413 Fragments arranged by size: 5413 435 315 138 BstYI r'GATC_y Cuts at: 1972 2448 2476 2578 3631 3998 4383 4400 5852 Size: 476 28 102 1053 367 385 17 1452 Cuts at: 5852 5938 5950 6296 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1053 476 385 367 346 102 86 28 17 12 Bsu36I CC'TnA_GG Cuts at: 3590 3590 Size: 6301 BtgI C'CryG_G Cuts at: 513 1065 2004 2685 2995 513 Size: 552 939 681 310 3819 Fragments arranged by size: 3819 939 681 552 310 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5562 Fragments arranged by size: 5562 683 56 BtsI GCAGTG_nn' Cuts at: 2253 3264 3310 3365 3528 3817 4611 4631 2253 Size: 1011 46 55 163 289 794 20 3923 Fragments arranged by size: 3923 1011 794 289 163 55 46 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6266 DraI TTT'AAA Cuts at: 3605 3605 Size: 6301 DraIII CAC_nnn'GTG Cuts at: 2428 2428 Size: 6301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5308 809 Size: 507 3992 1802 Fragments arranged by size: 3992 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2240 2457 2550 Size: 54 1425 50 275 18 410 217 93 Cuts at: 2550 2592 2688 2706 2727 2740 3150 3360 3748 Size: 42 96 18 21 13 410 210 388 Cuts at: 3748 4637 6266 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 410 410 388 275 217 210 96 93 54 50 43 42 21 18 18 13 EagI C'GGCC_G Cuts at: 1537 1812 2740 3748 1537 Size: 275 928 1008 4090 Fragments arranged by size: 4090 1008 928 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2210 2341 2362 2364 4231 1353 Size: 857 131 21 2 1867 3423 Fragments arranged by size: 3423 1867 857 131 21 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3412 4888 5262 5408 387 Size: 1064 138 1823 1476 374 146 1280 Fragments arranged by size: 1823 1476 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2766 2766 Size: 6301 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2492 2582 2666 2852 2894 2898 Size: 393 473 387 90 84 186 42 4 Cuts at: 2898 3047 4364 4961 5748 6016 1239 Size: 149 1317 597 787 268 1524 Fragments arranged by size: 1524 1317 787 597 473 393 387 268 186 149 90 84 42 4 EcoNI CCTnn'n_nnAGG Cuts at: 2552 2717 2552 Size: 165 6136 EcoO109I rG'GnC_Cy Cuts at: 2402 2586 2587 2935 2980 2981 3029 2402 Size: 184 1 348 45 1 48 5674 Fragments arranged by size: 5674 348 184 48 45 1 1 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 FseI GG_CCGG'CC Cuts at: 2242 2242 Size: 6301 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 2281 2622 2831 3266 5448 1678 Size: 603 341 209 435 2182 2531 Fragments arranged by size: 2531 2182 603 435 341 209 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2291 2323 3404 3410 3436 Size: 32 135 32 719 32 1081 6 26 Cuts at: 3436 3442 4942 4974 5016 5048 1373 Size: 6 1500 32 42 32 2626 Fragments arranged by size: 2626 1500 1081 719 135 42 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3645 3891 669 Size: 988 281 1707 246 3079 Fragments arranged by size: 3079 1707 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6301 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KasI G'GCGC_C Cuts at: 3262 3262 Size: 6301 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 3900 3900 Size: 6301 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3435 5415 5599 1305 Size: 2130 1980 184 2007 Fragments arranged by size: 2130 2007 1980 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2459 2552 2690 2708 2729 Size: 54 1425 343 627 93 138 18 21 Cuts at: 2729 10 Size: 3582 Fragments arranged by size: 3582 1425 627 343 138 93 54 21 18 MslI CAynn'nnrTG Cuts at: 518 1166 2258 2994 3572 4259 4618 4777 518 Size: 648 1092 736 578 687 359 159 2042 Fragments arranged by size: 2042 1092 736 687 648 578 359 159 MspA1I CmG'CkG Cuts at: 1606 2130 2757 2943 3204 3717 4394 5542 5787 Size: 524 627 186 261 513 677 1148 245 Cuts at: 5787 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 627 524 513 261 245 186 NaeI GCC'GGC Cuts at: 2240 2276 2240 Size: 36 6265 NarI GG'CG_CC Cuts at: 3263 3263 Size: 6301 NcoI C'CATG_G Cuts at: 513 1065 2004 2685 2995 513 Size: 552 939 681 310 3819 Fragments arranged by size: 3819 939 681 552 310 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NgoMIV G'CCGG_C Cuts at: 2238 2274 2238 Size: 36 6265 NheI G'CTAG_C Cuts at: 1052 3244 1052 Size: 2192 4109 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 3100 3659 5204 1132 Size: 97 1871 559 1545 2229 Fragments arranged by size: 2229 1871 1545 559 97 PasI CC'CwG_GG Cuts at: 1348 3167 1348 Size: 1819 4482 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 3070 3167 1108 Size: 1344 618 97 4242 Fragments arranged by size: 4242 1344 618 97 PfoI T'CCnGG_A Cuts at: 2151 2914 2151 Size: 763 5538 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2402 2402 Size: 6301 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PspOMI G'GGCC_C Cuts at: 2586 2980 2586 Size: 394 5907 PstI C_TGCA'G Cuts at: 830 2273 3653 830 Size: 1443 1380 3478 Fragments arranged by size: 3478 1443 1380 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 2130 2757 2130 Size: 627 5674 SacI G_AGCT'C Cuts at: 2768 2768 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SanDI GG'GwC_CC Cuts at: 2402 2402 Size: 6301 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SexAI A'CCwGG_T Cuts at: 2091 2091 Size: 6301 SfcI C'TryA_G Cuts at: 826 1045 2269 3649 4784 5465 5656 826 Size: 219 1224 1380 1135 681 191 1471 Fragments arranged by size: 1471 1380 1224 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3206 3206 Size: 6301 SfoI GGC'GCC Cuts at: 3264 3264 Size: 6301 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 2408 3103 3628 1872 Size: 536 695 525 4545 Fragments arranged by size: 4545 695 536 525 SmlI C'TyrA_G Cuts at: 820 1017 1943 2288 4405 5306 5568 820 Size: 197 926 345 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 345 262 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3100 3659 1132 Size: 1968 559 3774 Fragments arranged by size: 3774 1968 559 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 2634 2666 1517 Size: 1117 32 5152 Fragments arranged by size: 5152 1117 32 StyI C'CwwG_G Cuts at: 513 1065 2004 2243 2553 2658 2685 2995 3138 Size: 552 939 239 310 105 27 310 143 Cuts at: 3138 3740 513 Size: 602 3074 Fragments arranged by size: 3074 939 602 552 310 310 239 143 105 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2439 3008 3009 3058 3062 3283 4328 Size: 1255 859 569 1 49 4 221 1045 Cuts at: 4328 4345 4498 4683 5128 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1045 859 569 445 221 185 153 49 17 4 1 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4547 6259 96 Size: 274 80 33 51 1443 2570 1712 138 Fragments arranged by size: 2570 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6261 6293 6261 Size: 32 6269 Tth111I GACn'n_nGTC Cuts at: 1316 3293 1316 Size: 1977 4324 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 2406 3101 3626 1870 Size: 536 695 525 4545 Fragments arranged by size: 4545 695 536 525 XmnI GAAnn'nnTTC Cuts at: 4430 4430 Size: 6301 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3526 4109 276 Size: 53 83 186 936 1992 583 2468 Fragments arranged by size: 2468 1992 936 583 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SrfI SwaI XcmI