(Circular) (Six-base) MAPSORT of: pFN21AB9740.seq Check: 3523 from: 1 to: 6256 pFN21AB9740 6256 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2437 3433 2437 Size: 996 5260 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3219 3599 1937 Size: 1282 380 4594 Fragments arranged by size: 4594 1282 380 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 4319 5703 5971 1632 Size: 1010 1677 1384 268 1917 Fragments arranged by size: 1917 1677 1384 1010 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 2134 4985 2134 Size: 2851 3405 AleI CACnn'nnGTG Cuts at: 1166 2156 2235 3002 3254 1166 Size: 990 79 767 252 4168 Fragments arranged by size: 4168 990 767 252 79 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 2140 3055 3520 3999 5571 2140 Size: 915 465 479 1572 2825 Fragments arranged by size: 2825 1572 915 479 465 ApaI G_GGCC'C Cuts at: 2339 2339 Size: 6256 ApaLI G'TGCA_C Cuts at: 3525 4313 5469 3525 Size: 788 1156 4312 Fragments arranged by size: 4312 1156 788 ApoI r'AATT_y Cuts at: 1493 3565 3791 3943 6052 1493 Size: 2072 226 152 2109 1697 Fragments arranged by size: 2109 2072 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2316 2458 3271 3409 3581 1870 Size: 73 180 193 142 813 138 172 4545 Fragments arranged by size: 4545 813 193 180 172 142 138 73 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2237 2462 2949 3350 3497 Size: 325 217 627 450 225 487 401 147 Cuts at: 3497 3577 5032 618 Size: 80 1455 1842 Fragments arranged by size: 1842 1455 627 487 450 401 325 225 217 147 80 BanII G_rGCy'C Cuts at: 1391 2339 2812 3214 3575 1391 Size: 948 473 402 361 4072 Fragments arranged by size: 4072 948 473 402 361 BbeI G_GCGC'C Cuts at: 2953 3354 2953 Size: 401 5855 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5285 BbvCI CC'TCA_GC Cuts at: 3173 3173 Size: 6256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4447 4481 1903 Size: 34 2510 34 3678 Fragments arranged by size: 3678 2510 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2304 2493 4867 Size: 107 122 71 631 605 632 189 2374 Cuts at: 4867 136 Size: 1525 Fragments arranged by size: 2374 1525 632 631 605 189 122 107 71 BglII A'GATC_T Cuts at: 2658 6251 2658 Size: 3593 2663 BlpI GC'TnA_GC Cuts at: 3673 3673 Size: 6256 Bme1580I G_kGCm'C Cuts at: 1790 2339 2467 2477 3500 3529 4317 5473 1790 Size: 549 128 10 1023 29 788 1156 2573 Fragments arranged by size: 2573 1156 1023 788 549 128 29 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3356 3508 3987 Size: 416 53 83 399 24 1939 152 479 Cuts at: 3987 4945 442 Size: 958 1753 Fragments arranged by size: 1939 1753 958 479 416 399 152 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3105 3114 4916 1239 Size: 1866 9 1802 2579 Fragments arranged by size: 2579 1866 1802 9 Bpu10I CC'TnA_GC Cuts at: 3173 3173 Size: 6256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2538 4381 5246 5544 2538 Size: 1843 865 298 3250 Fragments arranged by size: 3250 1843 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3458 4919 882 Size: 617 1959 1461 2219 Fragments arranged by size: 2219 1959 1461 617 BsaAI yAC'GTr Cuts at: 493 1204 2536 4047 493 Size: 711 1332 1511 2702 Fragments arranged by size: 2702 1511 1332 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2950 3351 4063 4445 Size: 53 83 186 936 1417 401 712 382 Cuts at: 4445 5080 275 Size: 635 1451 Fragments arranged by size: 1451 1417 936 712 635 401 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2886 3475 4689 5087 5361 5508 5919 2886 Size: 589 1214 398 274 147 411 3223 Fragments arranged by size: 3223 1214 589 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6031 Fragments arranged by size: 6031 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2776 2855 2858 2921 3326 3496 3499 1186 Size: 1590 79 3 63 405 170 3 3943 Fragments arranged by size: 3943 1590 405 170 79 63 3 3 BseYI C'CCAG_C Cuts at: 2246 2299 2466 2526 3036 3048 3179 5459 5955 Size: 53 167 60 510 12 131 2280 496 Cuts at: 5955 2246 Size: 2547 Fragments arranged by size: 2547 2280 510 496 167 131 60 53 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2546 3071 3140 1163 Size: 573 810 525 69 4279 Fragments arranged by size: 4279 810 573 525 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2932 3706 4467 5495 1540 Size: 275 186 931 774 761 1028 2301 Fragments arranged by size: 2301 1028 931 774 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2682 3529 4317 4402 5473 2682 Size: 847 788 85 1071 3465 Fragments arranged by size: 3465 1071 847 788 85 BsmI GAATG_Cn' Cuts at: 2545 3767 3860 2545 Size: 1222 93 4941 Fragments arranged by size: 4941 1222 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2339 2467 2477 2682 2812 3214 3500 Size: 399 549 128 10 205 130 402 286 Cuts at: 3500 3529 3575 4317 4402 5473 1391 Size: 29 46 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 549 402 399 286 205 130 128 85 46 29 10 BspEI T'CCGG_A Cuts at: 3475 5919 3475 Size: 2444 3812 BspHI T'CATG_A Cuts at: 2670 4145 5875 2670 Size: 1475 1730 3051 Fragments arranged by size: 3051 1730 1475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2268 2437 3433 3611 844 Size: 1295 129 169 996 178 3489 Fragments arranged by size: 3489 1295 996 178 169 129 BsrBI CCG'CTC Cuts at: 1394 1823 2452 2781 3187 4143 1394 Size: 429 629 329 406 956 3507 Fragments arranged by size: 3507 956 629 429 406 329 BsrDI GCAATG_nn' Cuts at: 65 1248 4751 4925 65 Size: 1183 3503 174 1396 Fragments arranged by size: 3503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2931 2946 4900 5087 1700 Size: 110 1121 15 1954 187 2869 Fragments arranged by size: 2869 1954 1121 187 110 15 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 2799 3116 3163 794 Size: 2005 317 47 3887 Fragments arranged by size: 3887 2005 317 47 BstBI TT'CG_AA Cuts at: 2047 5060 2047 Size: 3013 3243 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6256 BstXI CCAn_nnnn'nTGG Cuts at: 3004 3004 Size: 6256 BstYI r'GATC_y Cuts at: 1972 2658 3586 3953 4338 4355 5807 5893 5905 Size: 686 928 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 928 686 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2904 3391 2904 Size: 487 5769 BtgI C'CryG_G Cuts at: 513 1065 2293 2376 2613 3009 3201 513 Size: 552 1228 83 237 396 192 3568 Fragments arranged by size: 3568 1228 552 396 237 192 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2124 487 Size: 683 56 898 4619 Fragments arranged by size: 4619 898 683 56 BtsI GCAGTG_nn' Cuts at: 2477 3152 3772 4566 4586 2477 Size: 675 620 794 20 4147 Fragments arranged by size: 4147 794 675 620 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2191 2253 2347 Size: 54 1425 50 275 18 361 62 94 Cuts at: 2347 2895 3006 3703 4592 6221 8 Size: 548 111 697 889 1629 43 Fragments arranged by size: 1629 1425 889 697 548 361 275 111 94 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2855 4186 1353 Size: 1502 1331 3423 Fragments arranged by size: 3423 1502 1331 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4843 5217 5363 387 Size: 1064 138 3254 374 146 1280 Fragments arranged by size: 3254 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2642 3105 3114 4319 4916 5703 5971 Size: 393 1010 463 9 1205 597 787 268 Cuts at: 5971 1239 Size: 1524 Fragments arranged by size: 1524 1205 1010 787 597 463 393 268 9 EcoNI CCTnn'n_nnAGG Cuts at: 3308 3308 Size: 6256 EcoO109I rG'GnC_Cy Cuts at: 2128 2336 2407 2869 2977 3387 2128 Size: 208 71 462 108 410 4997 Fragments arranged by size: 4997 462 410 208 108 71 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 6256 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 2953 3354 5403 1678 Size: 1275 401 2049 2531 Fragments arranged by size: 2531 2049 1275 401 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2120 2152 4897 Size: 32 135 32 437 32 79 32 2745 Cuts at: 4897 4929 4971 5003 1373 Size: 32 42 32 2626 Fragments arranged by size: 2745 2626 437 135 79 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3600 3846 669 Size: 988 281 1662 246 3079 Fragments arranged by size: 3079 1662 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KasI G'GCGC_C Cuts at: 2949 3350 2949 Size: 401 5855 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2892 2952 5370 5554 1305 Size: 1587 60 2418 184 2007 Fragments arranged by size: 2418 2007 1587 184 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 2349 2897 10 Size: 54 1425 343 517 548 3369 Fragments arranged by size: 3369 1425 548 517 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2156 2235 3002 3242 3254 4214 4573 Size: 648 990 79 767 240 12 960 359 Cuts at: 4573 4732 518 Size: 159 2042 Fragments arranged by size: 2042 990 960 767 648 359 240 159 79 12 MspA1I CmG'CkG Cuts at: 1606 2601 3672 4349 5497 5742 1606 Size: 995 1071 677 1148 245 2120 Fragments arranged by size: 2120 1148 1071 995 677 245 NaeI GCC'GGC Cuts at: 2948 2948 Size: 6256 NarI GG'CG_CC Cuts at: 2950 3351 2950 Size: 401 5855 NcoI C'CATG_G Cuts at: 513 1065 2293 2613 3201 513 Size: 552 1228 320 588 3568 Fragments arranged by size: 3568 1228 588 552 320 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NgoMIV G'CCGG_C Cuts at: 2946 2946 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2267 2326 3614 5159 1132 Size: 97 1038 59 1288 1545 2229 Fragments arranged by size: 2229 1545 1288 1038 97 59 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6256 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 3520 1108 Size: 2412 3844 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PmlI CAC'GTG Cuts at: 2536 2536 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2128 2407 2869 2128 Size: 279 462 5515 Fragments arranged by size: 5515 462 279 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PspOMI G'GGCC_C Cuts at: 2335 2335 Size: 6256 PstI C_TGCA'G Cuts at: 830 2136 2178 2406 3075 3297 3608 830 Size: 1306 42 228 669 222 311 3478 Fragments arranged by size: 3478 1306 669 311 228 222 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 2601 2601 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SbfI CC_TGCA'GG Cuts at: 2406 3608 2406 Size: 1202 5054 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SexAI A'CCwGG_T Cuts at: 3093 3093 Size: 6256 SfcI C'TryA_G Cuts at: 826 1045 2132 2174 2402 2662 3071 3216 3293 Size: 219 1087 42 228 260 409 145 77 Cuts at: 3293 3543 3604 4739 5420 5611 826 Size: 250 61 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 681 409 260 250 228 219 191 145 77 61 42 SfiI GGCCn_nnn'nGGCC Cuts at: 2493 2493 Size: 6256 SfoI GGC'GCC Cuts at: 2951 3352 2951 Size: 401 5855 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 2125 2460 3583 1872 Size: 253 335 1123 4545 Fragments arranged by size: 4545 1123 335 253 SmlI C'TyrA_G Cuts at: 820 1017 1943 2553 4360 5261 5523 820 Size: 197 926 610 1807 901 262 1553 Fragments arranged by size: 1807 1553 926 901 610 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 2326 3614 1132 Size: 1194 1288 3774 Fragments arranged by size: 3774 1288 1194 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 3396 1517 Size: 1879 4377 StyI C'CwwG_G Cuts at: 513 1065 2241 2293 2613 3201 3695 513 Size: 552 1176 52 320 588 494 3074 Fragments arranged by size: 3074 1176 588 552 494 320 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2195 2247 2450 3512 4283 4300 4453 Size: 1255 615 52 203 1062 771 17 153 Cuts at: 4453 4638 5083 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1062 771 615 445 203 185 153 52 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 4502 6214 Size: 274 80 33 51 1443 444 2081 1712 Cuts at: 6214 96 Size: 138 Fragments arranged by size: 2081 1712 1443 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2266 2298 6216 6248 2266 Size: 32 3918 32 2274 Fragments arranged by size: 3918 2274 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3064 1316 Size: 1748 4508 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2393 3007 2393 Size: 614 5642 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 2123 2458 3581 1870 Size: 253 335 1123 4545 Fragments arranged by size: 4545 1123 335 253 XmnI GAAnn'nnTTC Cuts at: 4385 4385 Size: 6256 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SrfI SwaI