Restriction map for
pFN21AB9740
(Linear) (Six-base) MAPSORT of: KIBB9740.seq Check: 5200 from: 1 to: 1551
KIBB9740.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 432 1428 1551
Size: 432 996 123
Fragments arranged by size:
996 432 123
AccI GT'mk_AC
Cuts at: 0 1214 1551
Size: 1214 337
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1551
Size: 637 914
AhdI GACnn_n'nnGTC
Cuts at: 0 129 1551
Size: 129 1422
AleI CACnn'nnGTG
Cuts at: 0 151 230 997 1249 1551
Size: 151 79 767 252 302
Fragments arranged by size:
767 302 252 151 79
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1050 1515 1551
Size: 135 915 465 36
Fragments arranged by size:
915 465 135 36
ApaI G_GGCC'C
Cuts at: 0 334 1551
Size: 334 1217
ApaLI G'TGCA_C
Cuts at: 0 1520 1551
Size: 1520 31
AvaI C'yCGr_G
Cuts at: 0 118 311 453 1266 1404 1551
Size: 118 193 142 813 138 147
Fragments arranged by size:
813 193 147 142 138 118
BanI G'GyrC_C
Cuts at: 0 232 457 944 1345 1492 1551
Size: 232 225 487 401 147 59
Fragments arranged by size:
487 401 232 225 147 59
BanII G_rGCy'C
Cuts at: 0 334 807 1209 1551
Size: 334 473 402 342
Fragments arranged by size:
473 402 342 334
BbeI G_GCGC'C
Cuts at: 0 948 1349 1551
Size: 948 401 202
Fragments arranged by size:
948 401 202
BbvCI CC'TCA_GC
Cuts at: 0 1168 1551
Size: 1168 383
BglI GCCn_nnn'nGGC
Cuts at: 0 299 488 1551
Size: 299 189 1063
Fragments arranged by size:
1063 299 189
BglII A'GATC_T
Cuts at: 0 653 1551
Size: 653 898
Bme1580I G_kGCm'C
Cuts at: 0 334 462 472 1495 1524 1551
Size: 334 128 10 1023 29 27
Fragments arranged by size:
1023 334 128 29 27 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 1351 1503 1551
Size: 1351 152 48
Fragments arranged by size:
1351 152 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 1109 1551
Size: 1100 9 442
Fragments arranged by size:
1100 442 9
Bpu10I CC'TnA_GC
Cuts at: 0 1168 1551
Size: 1168 383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1551
Size: 533 1018
BsaI GGTCTCn'nnnn_
Cuts at: 0 1453 1551
Size: 1453 98
BsaAI yAC'GTr
Cuts at: 0 531 1551
Size: 531 1020
BsaHI Gr'CG_yC
Cuts at: 0 945 1346 1551
Size: 945 401 205
Fragments arranged by size:
945 401 205
BsaWI w'CCGG_w
Cuts at: 0 881 1470 1551
Size: 881 589 81
Fragments arranged by size:
881 589 81
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 771 850 853 916 1321 1491 1494 1551
Size: 771 79 3 63 405 170 3 57
Fragments arranged by size:
771 405 170 79 63 57 3 3
BseYI C'CCAG_C
Cuts at: 0 241 294 461 521 1031 1043 1174 1551
Size: 241 53 167 60 510 12 131 377
Fragments arranged by size:
510 377 241 167 131 60 53 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 1066 1135 1551
Size: 541 525 69 416
Fragments arranged by size:
541 525 416 69
BsiEI CG_ry'CG
Cuts at: 0 927 1551
Size: 927 624
BsiHKAI G_wGCw'C
Cuts at: 0 677 1524 1551
Size: 677 847 27
Fragments arranged by size:
847 677 27
BsmI GAATG_Cn'
Cuts at: 0 540 1551
Size: 540 1011
Bsp1286I G_dGCh'C
Cuts at: 0 334 462 472 677 807 1209 1495 1524
Size: 334 128 10 205 130 402 286 29
Cuts at: 1524 1551
Size: 27
Fragments arranged by size:
402 334 286 205 130 128 29 27
10
BspEI T'CCGG_A
Cuts at: 0 1470 1551
Size: 1470 81
BspHI T'CATG_A
Cuts at: 0 665 1551
Size: 665 886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 263 432 1428 1551
Size: 134 129 169 996 123
Fragments arranged by size:
996 169 134 129 123
BsrBI CCG'CTC
Cuts at: 0 447 776 1182 1551
Size: 447 329 406 369
Fragments arranged by size:
447 406 369 329
BsrFI r'CCGG_y
Cuts at: 0 926 941 1551
Size: 926 15 610
Fragments arranged by size:
926 610 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 794 1111 1158 1551
Size: 794 317 47 393
Fragments arranged by size:
794 393 317 47
BstBI TT'CG_AA
Cuts at: 0 42 1551
Size: 42 1509
BstXI CCAn_nnnn'nTGG
Cuts at: 0 999 1551
Size: 999 552
BstYI r'GATC_y
Cuts at: 0 653 1551
Size: 653 898
Bsu36I CC'TnA_GG
Cuts at: 0 899 1386 1551
Size: 899 487 165
Fragments arranged by size:
899 487 165
BtgI C'CryG_G
Cuts at: 0 288 371 608 1004 1196 1551
Size: 288 83 237 396 192 355
Fragments arranged by size:
396 355 288 237 192 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 119 1551
Size: 119 1432
BtsI GCAGTG_nn'
Cuts at: 0 472 1147 1551
Size: 472 675 404
Fragments arranged by size:
675 472 404
EaeI y'GGCC_r
Cuts at: 0 186 248 342 890 1001 1551
Size: 186 62 94 548 111 550
Fragments arranged by size:
550 548 186 111 94 62
EarI CTCTTCn'nnn_
Cuts at: 0 850 1551
Size: 850 701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1100 1109 1551
Size: 637 463 9 442
Fragments arranged by size:
637 463 442 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1303 1551
Size: 1303 248
EcoO109I rG'GnC_Cy
Cuts at: 0 123 331 402 864 972 1382 1551
Size: 123 208 71 462 108 410 169
Fragments arranged by size:
462 410 208 169 123 108 71
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 693 1551
Size: 693 858
HaeII r_GCGC'y
Cuts at: 0 948 1349 1551
Size: 948 401 202
Fragments arranged by size:
948 401 202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 115 147 1551
Size: 4 32 79 32 1404
Fragments arranged by size:
1404 79 32 32 4
KasI G'GCGC_C
Cuts at: 0 944 1345 1551
Size: 944 401 206
Fragments arranged by size:
944 401 206
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 947 1551
Size: 887 60 604
Fragments arranged by size:
887 604 60
MscI TGG'CCA
Cuts at: 0 344 892 1551
Size: 344 548 659
Fragments arranged by size:
659 548 344
MslI CAynn'nnrTG
Cuts at: 0 151 230 997 1237 1249 1551
Size: 151 79 767 240 12 302
Fragments arranged by size:
767 302 240 151 79 12
MspA1I CmG'CkG
Cuts at: 0 596 1551
Size: 596 955
NaeI GCC'GGC
Cuts at: 0 943 1551
Size: 943 608
NarI GG'CG_CC
Cuts at: 0 945 1346 1551
Size: 945 401 205
Fragments arranged by size:
945 401 205
NcoI C'CATG_G
Cuts at: 0 288 608 1196 1551
Size: 288 320 588 355
Fragments arranged by size:
588 355 320 288
NgoMIV G'CCGG_C
Cuts at: 0 941 1551
Size: 941 610
NspI r_CATG'y
Cuts at: 0 262 321 1551
Size: 262 59 1230
Fragments arranged by size:
1230 262 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 1515 1551
Size: 1515 36
PmlI CAC'GTG
Cuts at: 0 531 1551
Size: 531 1020
PpuMI rG'GwC_Cy
Cuts at: 0 123 402 864 1551
Size: 123 279 462 687
Fragments arranged by size:
687 462 279 123
PspOMI G'GGCC_C
Cuts at: 0 330 1551
Size: 330 1221
PstI C_TGCA'G
Cuts at: 0 131 173 401 1070 1292 1551
Size: 131 42 228 669 222 259
Fragments arranged by size:
669 259 228 222 131 42
PvuII CAG'CTG
Cuts at: 0 596 1551
Size: 596 955
SbfI CC_TGCA'GG
Cuts at: 0 401 1551
Size: 401 1150
SexAI A'CCwGG_T
Cuts at: 0 1088 1551
Size: 1088 463
SfcI C'TryA_G
Cuts at: 0 127 169 397 657 1066 1211 1288 1538
Size: 127 42 228 260 409 145 77 250
Cuts at: 1538 1551
Size: 13
Fragments arranged by size:
409 260 250 228 145 127 77 42
13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 488 1551
Size: 488 1063
SfoI GGC'GCC
Cuts at: 0 946 1347 1551
Size: 946 401 204
Fragments arranged by size:
946 401 204
SmaI CCC'GGG
Cuts at: 0 120 455 1551
Size: 120 335 1096
Fragments arranged by size:
1096 335 120
SmlI C'TyrA_G
Cuts at: 0 548 1551
Size: 548 1003
SphI G_CATG'C
Cuts at: 0 321 1551
Size: 321 1230
StuI AGG'CCT
Cuts at: 0 1391 1551
Size: 1391 160
StyI C'CwwG_G
Cuts at: 0 236 288 608 1196 1551
Size: 236 52 320 588 355
Fragments arranged by size:
588 355 320 236 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 190 242 445 1507 1551
Size: 190 52 203 1062 44
Fragments arranged by size:
1062 203 190 52 44
TatI w'GTAC_w
Cuts at: 0 416 1551
Size: 416 1135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 261 293 1551
Size: 261 32 1258
Fragments arranged by size:
1258 261 32
Tth111I GACn'n_nGTC
Cuts at: 0 1059 1551
Size: 1059 492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 388 1002 1551
Size: 388 614 549
Fragments arranged by size:
614 549 388
XmaI C'CCGG_G
Cuts at: 0 118 453 1551
Size: 118 335 1098
Fragments arranged by size:
1098 335 118
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbvCI BglI BglII Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PmlI
PpuMI PspOMI PstI PvuII SbfI SexAI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI
BplI BsaBI BsaXI BsiWI BsmBI BsrDI BsrGI BssHII
BssSI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI ScaI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI XmnI ZraI