(Circular) (Six-base) MAPSORT of: pFN21AB9737.seq Check: 4863 from: 1 to: 6205 pFN21AB9737 6205 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2623 2623 Size: 6205 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3548 1937 Size: 1611 4594 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2531 3308 4268 5652 5920 1632 Size: 899 777 960 1384 268 1917 Fragments arranged by size: 1917 1384 960 899 777 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 5104 6081 1203 Size: 3901 977 1327 Fragments arranged by size: 3901 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3197 1184 Size: 2013 4192 AlwNI CAG_nnn'CTG Cuts at: 3100 3109 3948 5520 3100 Size: 9 839 1572 3785 Fragments arranged by size: 3785 1572 839 9 ApaLI G'TGCA_C Cuts at: 4262 5418 4262 Size: 1156 5049 ApoI r'AATT_y Cuts at: 1493 2604 3514 3740 3892 6001 1493 Size: 1111 910 226 152 2109 1697 Fragments arranged by size: 2109 1697 1111 910 226 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 2077 3530 1870 Size: 73 134 1453 4545 Fragments arranged by size: 4545 1453 134 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2978 3011 2978 Size: 33 6172 BamHI G'GATC_C Cuts at: 3535 3902 3535 Size: 367 5838 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2022 2153 2195 2210 3526 Size: 325 217 627 235 131 42 15 1316 Cuts at: 3526 4981 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1316 627 325 235 217 131 42 15 BanII G_rGCy'C Cuts at: 1391 2033 2964 3162 3524 1391 Size: 642 931 198 362 4072 Fragments arranged by size: 4072 931 642 362 198 BbeI G_GCGC'C Cuts at: 2199 2199 Size: 6205 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5234 BbvCI CC'TCA_GC Cuts at: 2093 2910 2093 Size: 817 5388 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2222 2256 4396 4430 1903 Size: 34 285 34 2140 34 3678 Fragments arranged by size: 3678 2140 285 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3446 4097 5307 3446 Size: 651 1210 4344 Fragments arranged by size: 4344 1210 651 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2112 3218 4816 Size: 107 122 71 631 605 440 1106 1598 Cuts at: 4816 136 Size: 1525 Fragments arranged by size: 1598 1525 1106 631 605 440 122 107 71 BglII A'GATC_T Cuts at: 6200 6200 Size: 6205 BlpI GC'TnA_GC Cuts at: 2661 3140 3622 2661 Size: 479 482 5244 Fragments arranged by size: 5244 482 479 Bme1580I G_kGCm'C Cuts at: 1790 2156 3264 3354 3440 4266 5422 1790 Size: 366 1108 90 86 826 1156 2573 Fragments arranged by size: 2573 1156 1108 826 366 90 86 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2103 3936 4894 Size: 416 53 83 399 24 686 1833 958 Cuts at: 4894 442 Size: 1753 Fragments arranged by size: 1833 1753 958 686 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2953 2953 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2017 2978 3184 3302 3311 3362 3482 4865 Size: 778 961 206 118 9 51 120 1383 Cuts at: 4865 1239 Size: 2579 Fragments arranged by size: 2579 1383 961 778 206 120 118 51 9 Bpu10I CC'TnA_GC Cuts at: 2093 2594 2910 3444 2093 Size: 501 316 534 4854 Fragments arranged by size: 4854 534 501 316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2782 2990 3020 4330 5195 5493 2782 Size: 208 30 1310 865 298 3494 Fragments arranged by size: 3494 1310 865 298 208 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4868 882 Size: 617 3369 2219 Fragments arranged by size: 3369 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3996 493 Size: 711 2792 2702 Fragments arranged by size: 2792 2702 711 BsaBI GATnn'nnATC Cuts at: 2849 6199 2849 Size: 3350 2855 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 2196 4012 4394 Size: 53 83 186 936 591 72 1816 382 Cuts at: 4394 5029 275 Size: 635 1451 Fragments arranged by size: 1816 1451 936 635 591 382 186 83 72 53 BsaWI w'CCGG_w Cuts at: 4638 5036 5310 5457 5868 4638 Size: 398 274 147 411 4975 Fragments arranged by size: 4975 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2015 2045 1178 Size: 30 165 30 612 30 5338 Fragments arranged by size: 5338 612 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2945 3035 3170 1186 Size: 1759 90 135 4221 Fragments arranged by size: 4221 1759 135 90 BseYI C'CCAG_C Cuts at: 2203 2772 5408 5904 2203 Size: 569 2636 496 2504 Fragments arranged by size: 2636 2504 569 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3098 3245 1163 Size: 573 1362 147 4123 Fragments arranged by size: 4123 1362 573 147 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3213 3655 4416 5444 1540 Size: 275 186 1212 442 761 1028 2301 Fragments arranged by size: 2301 1212 1028 761 442 275 186 BsiHKAI G_wGCw'C Cuts at: 2937 2964 4266 4351 5422 2937 Size: 27 1302 85 1071 3720 Fragments arranged by size: 3720 1302 1071 85 27 BsmI GAATG_Cn' Cuts at: 3716 3809 3716 Size: 93 6112 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2156 2937 2964 3162 3264 3354 Size: 399 243 123 781 27 198 102 90 Cuts at: 3354 3440 3524 4266 4351 5422 1391 Size: 86 84 742 85 1071 2174 Fragments arranged by size: 2174 1071 781 742 399 243 198 123 102 90 86 85 84 27 BspEI T'CCGG_A Cuts at: 5868 5868 Size: 6205 BspHI T'CATG_A Cuts at: 2484 4094 5824 2484 Size: 1610 1730 2865 Fragments arranged by size: 2865 1730 1610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2623 2982 3117 3476 3560 844 Size: 1779 359 135 359 84 3489 Fragments arranged by size: 3489 1779 359 359 135 84 BsrBI CCG'CTC Cuts at: 1394 1823 2877 4092 1394 Size: 429 1054 1215 3507 Fragments arranged by size: 3507 1215 1054 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2328 4700 4874 65 Size: 1183 1080 2372 174 1396 Fragments arranged by size: 2372 1396 1183 1080 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4849 5036 1700 Size: 110 3039 187 2869 Fragments arranged by size: 3039 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3069 96 Size: 2973 3232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 2315 3116 4265 5277 2315 Size: 801 1149 1012 3243 Fragments arranged by size: 3243 1149 1012 801 BstAPI GCAn_nnn'nTGC Cuts at: 794 2217 2466 3109 3388 794 Size: 1423 249 643 279 3611 Fragments arranged by size: 3611 1423 643 279 249 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstXI CCAn_nnnn'nTGG Cuts at: 2170 2170 Size: 6205 BstYI r'GATC_y Cuts at: 1972 3079 3535 3902 4287 4304 5756 5842 5854 Size: 1107 456 367 385 17 1452 86 12 Cuts at: 5854 6200 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1107 456 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2703 2703 Size: 6205 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5653 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5466 Fragments arranged by size: 5466 683 56 BtsI GCAGTG_nn' Cuts at: 3122 3188 3438 3721 4515 4535 3122 Size: 66 250 283 794 20 4792 Fragments arranged by size: 4792 794 283 250 66 20 ClaI AT'CG_AT Cuts at: 1554 2085 1554 Size: 531 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2062 5212 809 Size: 507 746 3150 1802 Fragments arranged by size: 3150 1802 746 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2265 2424 3210 Size: 54 1425 50 275 18 435 159 786 Cuts at: 3210 3491 3652 4541 6170 8 Size: 281 161 889 1629 43 Fragments arranged by size: 1629 1425 889 786 435 281 275 161 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3210 3652 1537 Size: 275 1398 442 4090 Fragments arranged by size: 4090 1398 442 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3281 4135 1353 Size: 1928 854 3423 Fragments arranged by size: 3423 1928 854 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2962 2962 Size: 6205 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2017 2531 2978 3184 3302 3308 3311 Size: 393 385 514 447 206 118 6 3 Cuts at: 3311 3362 3482 4268 4865 5652 5920 1239 Size: 51 120 786 597 787 268 1524 Fragments arranged by size: 1524 787 786 597 514 447 393 385 268 206 120 118 51 6 3 EcoNI CCTnn'n_nnAGG Cuts at: 3307 3307 Size: 6205 EcoO109I rG'GnC_Cy Cuts at: 2719 3040 3106 2719 Size: 321 66 5818 Fragments arranged by size: 5818 321 66 EcoRI G'AATT_C Cuts at: 3514 3892 3514 Size: 378 5827 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2398 2398 Size: 6205 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 2199 2308 3151 5352 1678 Size: 521 109 843 2201 2531 Fragments arranged by size: 2531 2201 843 521 109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2318 2350 4846 4878 4920 Size: 32 135 32 746 32 2496 32 42 Cuts at: 4920 4952 1373 Size: 32 2626 Fragments arranged by size: 2626 2496 746 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3549 3795 669 Size: 988 281 1611 246 3079 Fragments arranged by size: 3079 1611 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KasI G'GCGC_C Cuts at: 2195 2195 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 5319 5503 1305 Size: 840 3174 184 2007 Fragments arranged by size: 3174 2007 840 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3493 10 Size: 54 1425 343 1661 2722 Fragments arranged by size: 2722 1661 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4163 4522 4681 518 Size: 648 2997 359 159 2042 Fragments arranged by size: 2997 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2130 2247 2418 2983 3196 3503 3621 Size: 431 93 117 171 565 213 307 118 Cuts at: 3621 4298 5446 5691 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 565 431 307 245 213 171 118 117 93 NarI GG'CG_CC Cuts at: 2196 2196 Size: 6205 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5653 NdeI CA'TA_TG Cuts at: 387 387 Size: 6205 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 2287 3389 3563 5108 1132 Size: 97 1058 1102 174 1545 2229 Fragments arranged by size: 2229 1545 1102 1058 174 97 PasI CC'CwG_GG Cuts at: 1348 2149 2809 1348 Size: 801 660 4744 Fragments arranged by size: 4744 801 660 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 3303 1108 Size: 2195 4010 PfoI T'CCnGG_A Cuts at: 3279 3279 Size: 6205 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3106 3106 Size: 6205 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2396 2428 2396 Size: 32 6173 PstI C_TGCA'G Cuts at: 830 2835 2982 3105 3129 3252 3557 830 Size: 2005 147 123 24 123 305 3478 Fragments arranged by size: 3478 2005 305 147 123 123 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 PvuII CAG'CTG Cuts at: 2037 2418 2983 3503 2037 Size: 381 565 520 4739 Fragments arranged by size: 4739 565 520 381 SacI G_AGCT'C Cuts at: 2964 2964 Size: 6205 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 2831 2978 3101 3125 3235 3248 3553 Size: 219 1786 147 123 24 110 13 305 Cuts at: 3553 4688 5369 5560 826 Size: 1135 681 191 1471 Fragments arranged by size: 1786 1471 1135 681 305 219 191 147 123 110 24 13 SfiI GGCCn_nnn'nGGCC Cuts at: 3218 3218 Size: 6205 SfoI GGC'GCC Cuts at: 2197 2197 Size: 6205 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 3532 1872 Size: 1660 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2797 3005 3035 4309 5210 5472 Size: 197 926 854 208 30 1274 901 262 Cuts at: 5472 820 Size: 1553 Fragments arranged by size: 1553 1274 926 901 854 262 208 197 30 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3563 1132 Size: 2431 3774 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 2378 2474 3302 1517 Size: 861 96 828 4420 Fragments arranged by size: 4420 861 828 96 StyI C'CwwG_G Cuts at: 513 1065 2398 3216 3297 3644 513 Size: 552 1333 818 81 347 3074 Fragments arranged by size: 3074 1333 818 552 347 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 2197 2763 3421 4232 4249 4402 Size: 1255 591 26 566 658 811 17 153 Cuts at: 4402 4587 5032 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 811 658 591 566 445 185 153 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2280 2412 3069 Size: 274 80 33 51 1443 303 132 657 Cuts at: 3069 4451 6163 96 Size: 1382 1712 138 Fragments arranged by size: 1712 1443 1382 657 303 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2015 2047 6165 6197 2015 Size: 32 4118 32 2023 Fragments arranged by size: 4118 2023 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6205 XbaI T'CTAG_A Cuts at: 3082 3541 3082 Size: 459 5746 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2108 2251 2980 3426 2108 Size: 143 729 446 4887 Fragments arranged by size: 4887 729 446 143 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 3530 1870 Size: 1660 4545 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SrfI SwaI