(Circular) (Six-base) MAPSORT of: pFN21AB9734.seq Check: 2241 from: 1 to: 6163 pFN21AB9734 6163 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3213 3213 Size: 6163 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2550 3973 278 Size: 53 83 186 936 1014 1423 2468 Fragments arranged by size: 2468 1423 1014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3506 1937 Size: 1569 4594 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2079 3244 4226 5610 5878 1632 Size: 447 1165 982 1384 268 1917 Fragments arranged by size: 1917 1384 1165 982 447 268 AfeI AGC'GCT Cuts at: 2083 2586 2083 Size: 503 5660 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5966 AflIII A'CryG_T Cuts at: 1203 3327 5062 6039 1203 Size: 2124 1735 977 1327 Fragments arranged by size: 2124 1735 1327 977 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 2734 4892 2734 Size: 2158 4005 AleI CACnn'nnGTG Cuts at: 1166 3287 1166 Size: 2121 4042 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AlwNI CAG_nnn'CTG Cuts at: 2518 3906 5478 2518 Size: 1388 1572 3203 Fragments arranged by size: 3203 1572 1388 ApaI G_GGCC'C Cuts at: 2650 2650 Size: 6163 ApaLI G'TGCA_C Cuts at: 3380 4220 5376 3380 Size: 840 1156 4167 Fragments arranged by size: 4167 1156 840 ApoI r'AATT_y Cuts at: 1493 3472 3698 3850 5959 1493 Size: 1979 226 152 2109 1697 Fragments arranged by size: 2109 1979 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 2821 3488 1870 Size: 73 878 667 4545 Fragments arranged by size: 4545 878 667 73 BamHI G'GATC_C Cuts at: 3493 3860 3493 Size: 367 5796 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2338 2501 2566 3084 3484 Size: 325 217 627 551 163 65 518 400 Cuts at: 3484 4939 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 551 518 400 325 217 163 65 BanII G_rGCy'C Cuts at: 1391 2650 2862 3195 3482 1391 Size: 1259 212 333 287 4072 Fragments arranged by size: 4072 1259 333 287 212 BbeI G_GCGC'C Cuts at: 2570 3088 2570 Size: 518 5645 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5192 BbvCI CC'TCA_GC Cuts at: 2243 2529 2844 2931 2243 Size: 286 315 87 5475 Fragments arranged by size: 5475 315 286 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4354 4388 1903 Size: 34 50 34 2333 34 3678 Fragments arranged by size: 3678 2333 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5265 4055 Size: 1210 4953 BclI T'GATC_A Cuts at: 1548 1548 Size: 6163 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2693 2966 3093 Size: 107 122 71 631 605 1021 273 127 Cuts at: 3093 4774 136 Size: 1681 1525 Fragments arranged by size: 1681 1525 1021 631 605 273 127 122 107 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 3580 3580 Size: 6163 Bme1580I G_kGCm'C Cuts at: 1790 2280 2506 2650 3384 4224 5380 1790 Size: 490 226 144 734 840 1156 2573 Fragments arranged by size: 2573 1156 840 734 490 226 144 BmgBI CAC'GTC Cuts at: 1318 3328 1318 Size: 2010 4153 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3894 4852 442 Size: 416 53 83 399 24 2477 958 1753 Fragments arranged by size: 2477 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 2231 4823 1239 Size: 918 74 2592 2579 Fragments arranged by size: 2592 2579 918 74 Bpu10I CC'TnA_GC Cuts at: 2015 2243 2529 2844 2931 2015 Size: 228 286 315 87 5247 Fragments arranged by size: 5247 315 286 228 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2173 3371 3401 4288 5153 5451 2173 Size: 1198 30 887 865 298 2885 Fragments arranged by size: 2885 1198 887 865 298 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4826 882 Size: 617 3327 2219 Fragments arranged by size: 3327 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3289 3954 493 Size: 711 2085 665 2702 Fragments arranged by size: 2702 2085 711 665 BsaBI GATnn'nnATC Cuts at: 6157 6157 Size: 6163 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2547 2567 3085 3970 Size: 53 83 186 936 1014 20 518 885 Cuts at: 3970 4352 4987 275 Size: 382 635 1451 Fragments arranged by size: 1451 1014 936 885 635 518 382 186 83 53 20 BsaWI w'CCGG_w Cuts at: 4596 4994 5268 5415 5826 4596 Size: 398 274 147 411 4933 Fragments arranged by size: 4933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2218 2248 2423 2453 1178 Size: 30 165 30 815 30 175 30 4888 Fragments arranged by size: 4888 815 175 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 3422 1186 Size: 847 1389 3927 Fragments arranged by size: 3927 1389 847 BseYI C'CCAG_C Cuts at: 2078 2157 2770 2789 2944 3439 5366 5862 2078 Size: 79 613 19 155 495 1927 496 2379 Fragments arranged by size: 2379 1927 613 496 495 155 79 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3006 1163 Size: 573 1270 4320 Fragments arranged by size: 4320 1270 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2766 3128 3337 3613 4374 5402 Size: 275 186 765 362 209 276 761 1028 Cuts at: 5402 1540 Size: 2301 Fragments arranged by size: 2301 1028 765 761 362 276 275 209 186 BsiHKAI G_wGCw'C Cuts at: 3195 3384 4224 4309 5380 3195 Size: 189 840 85 1071 3978 Fragments arranged by size: 3978 1071 840 189 85 BsmI GAATG_Cn' Cuts at: 3674 3767 3674 Size: 93 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2280 2506 2650 2862 3195 3384 3482 Size: 399 490 226 144 212 333 189 98 Cuts at: 3482 4224 4309 5380 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 490 399 333 226 212 189 144 98 85 BspEI T'CCGG_A Cuts at: 5826 5826 Size: 6163 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3213 3518 844 Size: 2369 305 3489 Fragments arranged by size: 3489 2369 305 BsrBI CCG'CTC Cuts at: 1394 1823 3412 4050 1394 Size: 429 1589 638 3507 Fragments arranged by size: 3507 1589 638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4658 4832 65 Size: 1183 3410 174 1396 Fragments arranged by size: 3410 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2126 2761 3302 4807 4994 1700 Size: 110 316 635 541 1505 187 2869 Fragments arranged by size: 2869 1505 635 541 316 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6163 BssHII G'CGCG_C Cuts at: 1925 3179 3347 3398 3400 1925 Size: 1254 168 51 2 4688 Fragments arranged by size: 4688 1254 168 51 2 BssSI C'ACGA_G Cuts at: 4223 5235 4223 Size: 1012 5151 BstAPI GCAn_nnn'nTGC Cuts at: 794 3111 794 Size: 2317 3846 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstYI r'GATC_y Cuts at: 1972 2372 2446 3493 3860 4245 4262 5714 5800 Size: 400 74 1047 367 385 17 1452 86 Cuts at: 5800 5812 6158 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1047 400 385 367 346 86 74 17 12 BtgI C'CryG_G Cuts at: 513 1065 2118 2964 3088 3122 513 Size: 552 1053 846 124 34 3554 Fragments arranged by size: 3554 1053 846 552 124 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3369 487 Size: 683 56 2143 3281 Fragments arranged by size: 3281 2143 683 56 BtsI GCAGTG_nn' Cuts at: 2280 2519 3016 3679 4473 4493 2280 Size: 239 497 663 794 20 3950 Fragments arranged by size: 3950 794 663 497 239 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6128 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2763 2916 Size: 54 1425 50 275 18 291 642 153 Cuts at: 2916 3125 3334 3610 4499 6128 8 Size: 209 209 276 889 1629 43 Fragments arranged by size: 1629 1425 889 642 291 276 275 209 209 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2763 3125 3334 3610 1537 Size: 275 951 362 209 276 4090 Fragments arranged by size: 4090 951 362 276 275 209 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 4093 1353 Size: 959 1781 3423 Fragments arranged by size: 3423 1781 959 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2415 2919 2984 3008 4750 5124 Size: 1064 138 826 504 65 24 1742 374 Cuts at: 5124 5270 387 Size: 146 1280 Fragments arranged by size: 1742 1280 1064 826 504 374 146 138 65 24 EcoICRI GAG'CTC Cuts at: 3193 3193 Size: 6163 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2157 2231 3244 4226 4823 5610 Size: 393 447 78 74 1013 982 597 787 Cuts at: 5610 5878 1239 Size: 268 1524 Fragments arranged by size: 1524 1013 982 787 597 447 393 268 78 74 EcoNI CCTnn'n_nnAGG Cuts at: 2139 2139 Size: 6163 EcoO109I rG'GnC_Cy Cuts at: 2216 2612 2646 2647 2796 2848 3421 2216 Size: 396 34 1 149 52 573 4958 Fragments arranged by size: 4958 573 396 149 52 34 1 EcoRI G'AATT_C Cuts at: 3472 3850 3472 Size: 378 5785 FspI TGC'GCA Cuts at: 3209 3293 4669 3209 Size: 84 1376 4703 Fragments arranged by size: 4703 1376 84 FspAI rTGC'GCAy Cuts at: 3293 3293 Size: 6163 HaeII r_GCGC'y Cuts at: 1678 2085 2457 2570 2588 2961 3088 5310 1678 Size: 407 372 113 18 373 127 2222 2531 Fragments arranged by size: 2531 2222 407 373 372 127 113 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2423 2455 2582 2614 2720 Size: 32 135 32 851 32 127 32 106 Cuts at: 2720 2752 4804 4836 4878 4910 1373 Size: 32 2052 32 42 32 2626 Fragments arranged by size: 2626 2052 851 135 127 106 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3507 3753 669 Size: 988 281 1569 246 3079 Fragments arranged by size: 3079 1569 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6163 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KasI G'GCGC_C Cuts at: 2566 3084 2566 Size: 518 5645 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3210 5277 5461 1305 Size: 1905 2067 184 2007 Fragments arranged by size: 2067 2007 1905 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2918 10 Size: 54 1425 343 1086 3255 Fragments arranged by size: 3255 1425 1086 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3287 4121 4480 4639 518 Size: 648 2121 834 359 159 2042 Fragments arranged by size: 2121 2042 834 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2120 2172 2253 2380 2745 2793 2815 2944 Size: 514 52 81 127 365 48 22 129 Cuts at: 2944 2966 3090 3124 3579 4256 5404 5649 1606 Size: 22 124 34 455 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 514 455 365 245 129 127 124 81 52 48 34 22 22 NaeI GCC'GGC Cuts at: 2763 2763 Size: 6163 NarI GG'CG_CC Cuts at: 2567 3085 2567 Size: 518 5645 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5611 NdeI CA'TA_TG Cuts at: 387 387 Size: 6163 NgoMIV G'CCGG_C Cuts at: 2761 2761 Size: 6163 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3125 3334 3610 3125 Size: 209 276 5678 Fragments arranged by size: 5678 276 209 NspI r_CATG'y Cuts at: 1132 1229 3521 5066 1132 Size: 97 2292 1545 2229 Fragments arranged by size: 2292 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2625 2651 2855 1348 Size: 1277 26 204 4656 Fragments arranged by size: 4656 1277 204 26 PciI A'CATG_T Cuts at: 5062 5062 Size: 6163 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6163 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 6163 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PmlI CAC'GTG Cuts at: 3289 3289 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 5745 5777 4232 Size: 32 1481 32 4618 Fragments arranged by size: 4618 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2612 2612 Size: 6163 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3733 3733 Size: 6163 PspOMI G'GGCC_C Cuts at: 2646 2646 Size: 6163 PstI C_TGCA'G Cuts at: 830 3515 830 Size: 2685 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 PvuII CAG'CTG Cuts at: 2172 2745 2172 Size: 573 5590 SacI G_AGCT'C Cuts at: 3195 3195 Size: 6163 SacII CC_GC'GG Cuts at: 2121 2967 3091 3125 2121 Size: 846 124 34 5159 Fragments arranged by size: 5159 846 124 34 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SanDI GG'GwC_CC Cuts at: 2612 2612 Size: 6163 SbfI CC_TGCA'GG Cuts at: 3515 3515 Size: 6163 ScaI AGT'ACT Cuts at: 4411 6123 4411 Size: 1712 4451 SfcI C'TryA_G Cuts at: 826 1045 2248 3511 4646 5327 5518 826 Size: 219 1203 1263 1135 681 191 1471 Fragments arranged by size: 1471 1263 1203 1135 681 219 191 SfoI GGC'GCC Cuts at: 2568 3086 2568 Size: 518 5645 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 3302 1700 Size: 1602 4561 SmaI CCC'GGG Cuts at: 1872 2823 3490 1872 Size: 951 667 4545 Fragments arranged by size: 4545 951 667 SmlI C'TyrA_G Cuts at: 820 1017 1943 2152 3386 3416 4267 5168 5430 Size: 197 926 209 1234 30 851 901 262 Cuts at: 5430 820 Size: 1553 Fragments arranged by size: 1553 1234 926 901 851 262 209 197 30 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 4087 5 Size: 47 4035 2081 Fragments arranged by size: 4035 2081 47 StuI AGG'CCT Cuts at: 1517 2334 2486 3140 1517 Size: 817 152 654 4540 Fragments arranged by size: 4540 817 654 152 StyI C'CwwG_G Cuts at: 513 1065 3066 3135 3602 513 Size: 552 2001 69 467 3074 Fragments arranged by size: 3074 2001 552 467 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3311 4190 4207 4360 4545 4990 325 Size: 1255 1731 879 17 153 185 445 1498 Fragments arranged by size: 1731 1498 1255 879 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4409 6121 96 Size: 274 80 33 51 1443 2432 1712 138 Fragments arranged by size: 2432 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2218 2250 6123 6155 2218 Size: 32 3873 32 2226 Fragments arranged by size: 3873 2226 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2594 1316 Size: 1278 4885 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2377 2475 2377 Size: 98 6065 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6163 XmaI C'CCGG_G Cuts at: 1870 2821 3488 1870 Size: 951 667 4545 Fragments arranged by size: 4545 951 667 XmnI GAAnn'nnTTC Cuts at: 4292 4292 Size: 6163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2548 3971 276 Size: 53 83 186 936 1014 1423 2468 Fragments arranged by size: 2468 1423 1014 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI NruI NsiI PacI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI