(Circular) (Six-base) MAPSORT of: pFN21AB9731.seq Check: 6479 from: 1 to: 6127 pFN21AB9731 6127 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2089 2694 2941 2089 Size: 605 247 5275 Fragments arranged by size: 5275 605 247 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2028 3470 1937 Size: 91 1442 4594 Fragments arranged by size: 4594 1442 91 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4190 5574 5842 1632 Size: 2558 1384 268 1917 Fragments arranged by size: 2558 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 2121 2280 5026 6003 1203 Size: 918 159 2746 977 1327 Fragments arranged by size: 2746 1327 977 918 159 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 2220 1166 Size: 1054 5073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6127 AlwNI CAG_nnn'CTG Cuts at: 2590 2839 3332 3870 5442 2590 Size: 249 493 538 1572 3275 Fragments arranged by size: 3275 1572 538 493 249 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 2084 3436 3662 3814 5923 1493 Size: 591 1352 226 152 2109 1697 Fragments arranged by size: 2109 1697 1352 591 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 2227 3452 1870 Size: 73 284 1225 4545 Fragments arranged by size: 4545 1225 284 73 AvrII C'CTAG_G Cuts at: 2737 2737 Size: 6127 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2222 3448 4903 618 Size: 325 217 627 351 84 1226 1455 1842 Fragments arranged by size: 1842 1455 1226 627 351 325 217 84 BanII G_rGCy'C Cuts at: 1391 2637 2671 3339 3446 1391 Size: 1246 34 668 107 4072 Fragments arranged by size: 4072 1246 668 107 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5156 BbvCI CC'TCA_GC Cuts at: 3239 3239 Size: 6127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2033 2067 4318 4352 1903 Size: 34 50 34 12 34 2251 34 3678 Fragments arranged by size: 3678 2251 50 34 34 34 34 12 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 2909 3544 2909 Size: 635 5492 Bme1580I G_kGCm'C Cuts at: 1790 4188 5344 1790 Size: 2398 1156 2573 Fragments arranged by size: 2573 2398 1156 BmgBI CAC'GTC Cuts at: 1318 2124 2728 1318 Size: 806 604 4717 Fragments arranged by size: 4717 806 604 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3858 4816 442 Size: 416 53 83 399 24 2441 958 1753 Fragments arranged by size: 2441 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4787 1239 Size: 3548 2579 Bpu10I CC'TnA_GC Cuts at: 3239 3325 3239 Size: 86 6041 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2339 4252 5117 5415 2339 Size: 1913 865 298 3051 Fragments arranged by size: 3051 1913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3290 4790 882 Size: 617 1791 1500 2219 Fragments arranged by size: 2219 1791 1500 617 BsaAI yAC'GTr Cuts at: 493 1204 3918 493 Size: 711 2714 2702 Fragments arranged by size: 2714 2702 711 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2256 2409 4560 4958 5232 5379 5790 2256 Size: 153 2151 398 274 147 411 2593 Fragments arranged by size: 2593 2151 411 398 274 153 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 2471 2501 1178 Size: 30 165 30 867 30 171 30 4804 Fragments arranged by size: 4804 867 171 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2275 1186 Size: 1089 5038 BseYI C'CCAG_C Cuts at: 2065 2620 3127 3140 3396 5330 5826 2065 Size: 555 507 13 256 1934 496 2366 Fragments arranged by size: 2366 1934 555 507 496 256 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2040 2117 2969 3155 1163 Size: 573 304 77 852 186 4135 Fragments arranged by size: 4135 852 573 304 186 77 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2980 3577 4338 5366 1540 Size: 275 186 979 597 761 1028 2301 Fragments arranged by size: 2301 1028 979 761 597 275 186 BsiHKAI G_wGCw'C Cuts at: 2637 2685 4188 4273 5344 2637 Size: 48 1503 85 1071 3420 Fragments arranged by size: 3420 1503 1071 85 48 BsmI GAATG_Cn' Cuts at: 3638 3731 3638 Size: 93 6034 BsmBI CGTCTCn'nnnn_ Cuts at: 2515 2515 Size: 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2637 2671 2685 3339 3446 4188 4273 Size: 399 847 34 14 654 107 742 85 Cuts at: 4273 5344 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 847 742 654 399 107 85 34 14 BspEI T'CCGG_A Cuts at: 2256 5790 2256 Size: 3534 2593 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2089 2694 2941 3482 844 Size: 1245 605 247 541 3489 Fragments arranged by size: 3489 1245 605 541 247 BsrBI CCG'CTC Cuts at: 1394 1823 2404 4014 1394 Size: 429 581 1610 3507 Fragments arranged by size: 3507 1610 581 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2122 2749 4622 4796 65 Size: 1183 874 627 1873 174 1396 Fragments arranged by size: 1873 1396 1183 874 627 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2301 4771 4958 1700 Size: 110 491 2470 187 2869 Fragments arranged by size: 2869 2470 491 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 2689 3121 3155 794 Size: 1895 432 34 3766 Fragments arranged by size: 3766 1895 432 34 BstBI TT'CG_AA Cuts at: 4931 4931 Size: 6127 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstXI CCAn_nnnn'nTGG Cuts at: 2164 2164 Size: 6127 BstYI r'GATC_y Cuts at: 1972 2708 3066 3457 3824 4209 4226 5678 5764 Size: 736 358 391 367 385 17 1452 86 Cuts at: 5764 5776 6122 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 736 391 385 367 358 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2205 2712 513 Size: 552 1140 507 3928 Fragments arranged by size: 3928 1140 552 507 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2539 3159 487 Size: 683 56 1313 620 3455 Fragments arranged by size: 3455 1313 683 620 56 BtsI GCAGTG_nn' Cuts at: 2312 2764 3643 4437 4457 2312 Size: 452 879 794 20 3982 Fragments arranged by size: 3982 879 794 452 20 ClaI AT'CG_AT Cuts at: 1554 2847 1554 Size: 1293 4834 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2753 5134 809 Size: 507 1437 2381 1802 Fragments arranged by size: 2381 1802 1437 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2998 3093 Size: 54 1425 50 275 18 210 958 95 Cuts at: 3093 3574 4463 6092 8 Size: 481 889 1629 43 Fragments arranged by size: 1629 1425 958 889 481 275 210 95 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3089 4057 1353 Size: 1736 968 3423 Fragments arranged by size: 3423 1736 968 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2492 2795 4714 5088 5234 387 Size: 1064 138 903 303 1919 374 146 1280 Fragments arranged by size: 1919 1280 1064 903 374 303 146 138 EcoICRI GAG'CTC Cuts at: 2635 2635 Size: 6127 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4190 4787 5574 5842 1239 Size: 393 2558 597 787 268 1524 Fragments arranged by size: 2558 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3113 3185 3113 Size: 72 6055 EcoO109I rG'GnC_Cy Cuts at: 2482 3135 2482 Size: 653 5474 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2269 2380 2269 Size: 111 6016 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 2178 5274 1678 Size: 500 3096 2531 Fragments arranged by size: 3096 2531 500 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2404 2436 2471 2503 4768 Size: 32 135 32 832 32 35 32 2265 Cuts at: 4768 4800 4842 4874 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2265 832 135 42 35 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2029 2106 3471 3717 669 Size: 988 281 91 77 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 91 77 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5241 5425 1305 Size: 3936 184 2007 Fragments arranged by size: 3936 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3095 10 Size: 54 1425 343 1263 3042 Fragments arranged by size: 3042 1425 1263 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2220 4085 4444 4603 518 Size: 648 870 184 1865 359 159 2042 Fragments arranged by size: 2042 1865 870 648 359 184 159 MspA1I CmG'CkG Cuts at: 1606 2587 3543 4220 5368 5613 1606 Size: 981 956 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 956 677 245 NaeI GCC'GGC Cuts at: 2303 2303 Size: 6127 NcoI C'CATG_G Cuts at: 513 1065 2712 513 Size: 552 1647 3928 Fragments arranged by size: 3928 1647 552 NdeI CA'TA_TG Cuts at: 387 3195 387 Size: 2808 3319 NgoMIV G'CCGG_C Cuts at: 2301 2301 Size: 6127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2284 3485 5030 1132 Size: 97 1055 1201 1545 2229 Fragments arranged by size: 2229 1545 1201 1055 97 PasI CC'CwG_GG Cuts at: 1348 3273 1348 Size: 1925 4202 PciI A'CATG_T Cuts at: 2280 5026 2280 Size: 2746 3381 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6127 PfoI T'CCnGG_A Cuts at: 2513 2832 2513 Size: 319 5808 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2482 3135 2482 Size: 653 5474 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PspXI vC'TCGA_Gb Cuts at: 2227 2227 Size: 6127 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2267 2299 2267 Size: 32 6095 PstI C_TGCA'G Cuts at: 830 2294 2351 2475 2844 3479 830 Size: 1464 57 124 369 635 3478 Fragments arranged by size: 3478 1464 635 369 124 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2587 2587 Size: 6127 SacI G_AGCT'C Cuts at: 2637 2637 Size: 6127 SalI G'TCGA_C Cuts at: 1936 2027 3469 1936 Size: 91 1442 4594 Fragments arranged by size: 4594 1442 91 SanDI GG'GwC_CC Cuts at: 3135 3135 Size: 6127 SapI GCTCTTCn'nnn_ Cuts at: 3089 3089 Size: 6127 SbfI CC_TGCA'GG Cuts at: 2475 3479 2475 Size: 1004 5123 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SexAI A'CCwGG_T Cuts at: 2649 2649 Size: 6127 SfcI C'TryA_G Cuts at: 826 1045 2047 2290 2347 2471 2840 3475 4610 Size: 219 1002 243 57 124 369 635 1135 Cuts at: 4610 5291 5482 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1002 681 635 369 243 219 191 124 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2227 2354 4231 5132 5394 820 Size: 197 926 284 127 1877 901 262 1553 Fragments arranged by size: 1877 1553 926 901 284 262 197 127 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2712 2737 2901 3566 513 Size: 552 1647 25 164 665 3074 Fragments arranged by size: 3074 1647 665 552 164 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2156 4154 4171 4324 4509 4954 325 Size: 1255 576 1998 17 153 185 445 1498 Fragments arranged by size: 1998 1498 1255 576 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 2613 4373 Size: 274 80 33 51 1443 306 330 1760 Cuts at: 4373 6085 96 Size: 1712 138 Fragments arranged by size: 1760 1712 1443 330 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 6087 6119 2270 Size: 32 3785 32 2278 Fragments arranged by size: 3785 2278 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2710 2710 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 2227 1943 Size: 284 5843 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 2363 4256 2363 Size: 1893 4234 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI RsrII SacII SfiI SfoI SrfI SwaI