Restriction map for
pFN21AB9727
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstNI BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRII EcoRV FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeII MfeI
MluI MluNI MroI MscI MunI
MvaI MvnI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Asp700I 784 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 968 GAGCAC G[AT]GC[AT]^C
BanI 698 GGCACC G^G[CT][GA]CC
BanII 553 GAGCCC G[GA]GC[CT]^C
BbsI 739 GAAGAC GAAGAC(2/6)
BclI 110 TGATCA T^GATCA
BglII 786 AGATCT A^GATCT
BpuAI 739 GAAGAC GAAGAC(2/6)
BsaBI 787 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 133 GAGGAG GAGGAG(10/8)
BsiHKAI 968 GAGCAC G[AT]GC[AT]^C
BsmFI 1287 GGGAC GGGAC(10/14)
BspMI 691 GCAGGT ACCTGC(4/8)
BssSI 982 CACGAG CACGAG(-5/-1)
BstAPI 664 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1181 CCATTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 786 AGATCT [GA]^GATC[CT]
DraI 195 TTTAAA TTT^AAA
DsaI 1004 CCATGG C^C[GA][CT]GG
EcoNI 439 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 563 ATGCAT ATGCA^T
FbaI 110 TGATCA T^GATCA
HgaI 907 GACGC GACGC(5/10)
MamI 787 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 786 AGATCT [GA]^GATC[CT]
NcoI 1004 CCATGG C^CATGG
NdeI 866 CATATG CA^TATG
NheI 682 GCTAGC G^CTAGC
NlaIV 698 GGCACC GG[ACGT]^[ACGT]CC
NsiI 563 ATGCAT ATGCA^T
PstI 242 CTGCAG CTGCA^G
ScaI 400 AGTACT AGT^ACT
SfaNI 514 GATGC GCATC(5/9)
SphI 87 GCATGC GCATG^C
StuI 829 AGGCCT AGG^CCT
XhoII 786 AGATCT [GA]^GATC[CT]
XmnI 784 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwI 63 GATCC GGATC(4/5)
436 GATCC
BsaI 343 GAGACC GGTCTC(1/5)
1069 GAGACC
DraII 726 AGGTCCT [GA]G^G[ACGT]CC[CT]
1304 AGGACCT
EaeI 1197 TGGCCG [CT]^GGCC[GA]
1231 CGGCCA
EcoO109I 726 AGGTCCT [GA]G^G[ACGT]CC[CT]
1304 AGGACCT
MaeIII 1036 GTGAC ^GT[ACGT]AC
1055 GTGAC
NspI 87 GCATGC [GA]CATG^[CT]
1295 GCATGT
PleI 1057 GACTC GAGTC(4/5)
1078 GAGTC
PpuMI 726 AGGTCCT [GA]G^G[AT]CC[CT]
1304 AGGACCT
PvuII 1025 CAGCTG CAG^CTG
1109 CAGCTG
SmlI 154 CTCAAG C^T[CT][GA]AG
610 CTTGAG
Tsp45I 1036 GTGAC ^GT[GC]AC
1055 GTGAC
XcmI 858 CCAAGGGGCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1181 CCATTGTCATGGTGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
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AciI AfaI AluI AvaII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstF5I Cac8I Cfr13I
DdeI DpnII DrdI EarI EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeI MboI
MboII MnlI MseI MslI MspA1I
MspI MwoI NciI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I