(Circular) (Six-base) MAPSORT of: pFN21AB9720.seq Check: 4405 from: 1 to: 5917 pFN21AB9720 5917 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2152 2228 2285 2857 2989 2152 Size: 76 57 572 132 5080 Fragments arranged by size: 5080 572 132 76 57 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2895 3260 1937 Size: 958 365 4594 Fragments arranged by size: 4594 958 365 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2620 3980 5364 5632 1632 Size: 988 1360 1384 268 1917 Fragments arranged by size: 1917 1384 1360 988 268 AfeI AGC'GCT Cuts at: 3050 3050 Size: 5917 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 2607 4816 5793 1203 Size: 1404 2209 977 1327 Fragments arranged by size: 2209 1404 1327 977 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AlwNI CAG_nnn'CTG Cuts at: 2524 3660 5232 2524 Size: 1136 1572 3209 Fragments arranged by size: 3209 1572 1136 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 3226 3452 3604 5713 1493 Size: 1733 226 152 2109 1697 Fragments arranged by size: 2109 1733 1697 226 152 AscI GG'CGCG_CC Cuts at: 2654 2654 Size: 5917 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 2431 2443 2488 2675 3208 3242 1870 Size: 73 488 12 45 187 533 34 4545 Fragments arranged by size: 4545 533 488 187 73 45 34 12 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 2414 2773 3057 3238 Size: 325 217 627 327 300 359 284 181 Cuts at: 3238 4693 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 359 327 325 300 284 217 181 BanII G_rGCy'C Cuts at: 1391 2450 2538 2730 3236 1391 Size: 1059 88 192 506 4072 Fragments arranged by size: 4072 1059 506 192 88 BbeI G_GCGC'C Cuts at: 2777 3061 2777 Size: 284 5633 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2206 3146 928 Size: 971 307 940 3699 Fragments arranged by size: 3699 971 940 307 BbvCI CC'TCA_GC Cuts at: 2267 2267 Size: 5917 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5019 3809 Size: 1210 4707 BclI T'GATC_A Cuts at: 1548 3159 1548 Size: 1611 4306 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 2445 4528 Size: 107 122 71 631 605 368 405 2083 Cuts at: 4528 136 Size: 1525 Fragments arranged by size: 2083 1525 631 605 405 368 122 107 71 BglII A'GATC_T Cuts at: 5912 5912 Size: 5917 BlpI GC'TnA_GC Cuts at: 2532 3334 2532 Size: 802 5115 Bme1580I G_kGCm'C Cuts at: 1790 3978 5134 1790 Size: 2188 1156 2573 Fragments arranged by size: 2573 2188 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3648 4606 442 Size: 416 53 83 399 24 2231 958 1753 Fragments arranged by size: 2231 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2113 2113 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2740 4577 1239 Size: 1501 1837 2579 Fragments arranged by size: 2579 1837 1501 Bpu10I CC'TnA_GC Cuts at: 2267 2714 2267 Size: 447 5470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2327 2903 4042 4907 5205 2327 Size: 576 1139 865 298 3039 Fragments arranged by size: 3039 1139 865 576 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2472 4580 882 Size: 617 973 2108 2219 Fragments arranged by size: 2219 2108 973 617 BsaAI yAC'GTr Cuts at: 493 1204 3708 493 Size: 711 2504 2702 Fragments arranged by size: 2702 2504 711 BsaBI GATnn'nnATC Cuts at: 5911 5911 Size: 5917 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2127 2774 2946 3058 Size: 53 83 186 936 594 647 172 112 Cuts at: 3058 3143 3724 4106 4741 275 Size: 85 581 382 635 1451 Fragments arranged by size: 1451 936 647 635 594 581 382 186 172 112 85 83 53 BsaWI w'CCGG_w Cuts at: 2316 4350 4748 5022 5169 5580 2316 Size: 2034 398 274 147 411 2653 Fragments arranged by size: 2653 2034 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5692 Fragments arranged by size: 5692 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2419 1186 Size: 973 260 4684 Fragments arranged by size: 4684 973 260 BseYI C'CCAG_C Cuts at: 2025 2593 2878 3039 3045 3111 5120 5616 2025 Size: 568 285 161 6 66 2009 496 2326 Fragments arranged by size: 2326 2009 568 496 285 161 66 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 2750 1163 Size: 573 666 348 4330 Fragments arranged by size: 4330 666 573 348 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 2153 2392 2553 2904 3367 Size: 275 186 87 65 239 161 351 463 Cuts at: 3367 4128 5156 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 463 351 275 239 186 161 87 65 BsiHKAI G_wGCw'C Cuts at: 2730 3978 4063 5134 2730 Size: 1248 85 1071 3513 Fragments arranged by size: 3513 1248 1071 85 BsmI GAATG_Cn' Cuts at: 3428 3521 3428 Size: 93 5824 BsmBI CGTCTCn'nnnn_ Cuts at: 2345 2345 Size: 5917 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2450 2538 2730 3236 3978 4063 5134 Size: 399 660 88 192 506 742 85 1071 Cuts at: 5134 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 660 506 399 192 88 85 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2152 2228 2285 2857 2881 2989 3272 844 Size: 1308 76 57 572 24 108 283 3489 Fragments arranged by size: 3489 1308 572 283 108 76 57 24 BsrBI CCG'CTC Cuts at: 1394 1823 2149 2196 2472 3804 1394 Size: 429 326 47 276 1332 3507 Fragments arranged by size: 3507 1332 429 326 276 47 BsrDI GCAATG_nn' Cuts at: 65 1248 4412 4586 65 Size: 1183 3164 174 1396 Fragments arranged by size: 3164 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 2280 2497 2683 2792 4561 4748 Size: 110 208 262 217 186 109 1769 187 Cuts at: 4748 1700 Size: 2869 Fragments arranged by size: 2869 1769 262 217 208 187 186 110 109 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 2080 2527 2654 2824 2851 3085 1925 Size: 155 447 127 170 27 234 4757 Fragments arranged by size: 4757 447 234 170 155 127 27 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 2407 2524 794 Size: 1613 117 4187 Fragments arranged by size: 4187 1613 117 BstBI TT'CG_AA Cuts at: 4721 4721 Size: 5917 BstEII G'GTnAC_C Cuts at: 1184 2014 1184 Size: 830 5087 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5917 BstYI r'GATC_y Cuts at: 1972 3247 3614 3999 4016 5468 5554 5566 5912 Size: 1275 367 385 17 1452 86 12 346 Cuts at: 5912 1972 Size: 1977 Fragments arranged by size: 1977 1452 1275 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2418 2643 2829 513 Size: 552 939 6 408 225 186 3601 Fragments arranged by size: 3601 939 552 408 225 186 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2911 487 Size: 683 56 1685 3493 Fragments arranged by size: 3493 1685 683 56 BtsI GCAGTG_nn' Cuts at: 2469 3433 4227 4247 2469 Size: 964 794 20 4139 Fragments arranged by size: 4139 964 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4924 809 Size: 507 3608 1802 Fragments arranged by size: 3608 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2085 2150 Size: 54 1425 50 275 18 177 78 65 Cuts at: 2150 2389 2421 2550 2688 2784 2814 2901 3364 Size: 239 32 129 138 96 30 87 463 Cuts at: 3364 4253 5882 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 463 275 239 177 138 129 96 87 78 65 54 50 43 32 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2085 2150 2389 2550 2901 3364 1537 Size: 275 273 65 239 161 351 463 4090 Fragments arranged by size: 4090 463 351 275 273 239 161 65 EarI CTCTTCn'nnn_ Cuts at: 1353 2489 2639 2735 2939 3847 1353 Size: 1136 150 96 204 908 3423 Fragments arranged by size: 3423 1136 908 204 150 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2246 2490 4504 4878 5024 387 Size: 1064 138 657 244 2014 374 146 1280 Fragments arranged by size: 2014 1280 1064 657 374 244 146 138 EcoICRI GAG'CTC Cuts at: 2728 2728 Size: 5917 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2620 2740 3980 4577 5364 5632 1239 Size: 393 988 120 1240 597 787 268 1524 Fragments arranged by size: 1524 1240 988 787 597 393 268 120 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 5917 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 2456 2705 2759 2777 3052 3061 5064 1678 Size: 778 249 54 18 275 9 2003 2531 Fragments arranged by size: 2531 2003 778 275 249 54 18 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 4558 4590 4632 Size: 32 135 32 515 32 2439 32 42 Cuts at: 4632 4664 1373 Size: 32 2626 Fragments arranged by size: 2626 2439 515 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2896 3261 3507 669 Size: 988 281 958 365 246 3079 Fragments arranged by size: 3079 988 958 365 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KasI G'GCGC_C Cuts at: 2773 3057 2773 Size: 284 5633 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2310 5031 5215 1305 Size: 1005 2721 184 2007 Fragments arranged by size: 2721 2007 1005 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3918 Fragments arranged by size: 3918 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2208 3875 4234 4393 518 Size: 648 843 199 1667 359 159 2042 Fragments arranged by size: 2042 1667 843 648 359 199 159 MspA1I CmG'CkG Cuts at: 1606 2521 3115 3333 4010 5158 5403 1606 Size: 915 594 218 677 1148 245 2120 Fragments arranged by size: 2120 1148 915 677 594 245 218 NaeI GCC'GGC Cuts at: 2794 2794 Size: 5917 NarI GG'CG_CC Cuts at: 2774 3058 2774 Size: 284 5633 NcoI C'CATG_G Cuts at: 513 1065 2004 2010 513 Size: 552 939 6 4420 Fragments arranged by size: 4420 939 552 6 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NgoMIV G'CCGG_C Cuts at: 2792 2792 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 2389 3364 2389 Size: 975 4942 NspI r_CATG'y Cuts at: 1132 1229 3172 3275 4820 1132 Size: 97 1943 103 1545 2229 Fragments arranged by size: 2229 1943 1545 103 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5917 PfoI T'CCnGG_A Cuts at: 2859 2859 Size: 5917 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PstI C_TGCA'G Cuts at: 830 2163 2892 2988 3269 830 Size: 1333 729 96 281 3478 Fragments arranged by size: 3478 1333 729 281 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 PvuII CAG'CTG Cuts at: 2521 3115 2521 Size: 594 5323 SacI G_AGCT'C Cuts at: 2730 2730 Size: 5917 SalI G'TCGA_C Cuts at: 1936 2894 3259 1936 Size: 958 365 4594 Fragments arranged by size: 4594 958 365 SapI GCTCTTCn'nnn_ Cuts at: 2639 2735 2939 2639 Size: 96 204 5617 Fragments arranged by size: 5617 204 96 SbfI CC_TGCA'GG Cuts at: 2892 2988 3269 2892 Size: 96 281 5540 Fragments arranged by size: 5540 281 96 ScaI AGT'ACT Cuts at: 2333 4165 5877 2333 Size: 1832 1712 2373 Fragments arranged by size: 2373 1832 1712 SexAI A'CCwGG_T Cuts at: 2694 2844 2694 Size: 150 5767 SfcI C'TryA_G Cuts at: 826 1045 2159 2888 2984 3265 4400 5081 5272 Size: 219 1114 729 96 281 1135 681 191 Cuts at: 5272 826 Size: 1471 Fragments arranged by size: 1471 1135 1114 729 681 281 219 191 96 SfoI GGC'GCC Cuts at: 2775 3059 2775 Size: 284 5633 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 2445 2490 3244 1872 Size: 573 45 754 4545 Fragments arranged by size: 4545 754 573 45 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 2918 4021 4922 5184 820 Size: 197 926 399 576 1103 901 262 1553 Fragments arranged by size: 1553 1103 926 901 576 399 262 197 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5917 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 3356 513 Size: 552 939 6 1346 3074 Fragments arranged by size: 3074 1346 939 552 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3944 3961 4114 4299 4744 325 Size: 1255 2364 17 153 185 445 1498 Fragments arranged by size: 2364 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2331 4163 5875 Size: 274 80 33 51 1443 354 1832 1712 Cuts at: 5875 96 Size: 138 Fragments arranged by size: 1832 1712 1443 354 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5877 5909 5877 Size: 32 5885 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 2443 2488 3242 1870 Size: 573 45 754 4545 Fragments arranged by size: 4545 754 573 45 XmnI GAAnn'nnTTC Cuts at: 2351 4046 2351 Size: 1695 4222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI