Restriction map for
pFN21AB9716
(Circular) (Six-base) MAPSORT of: pFN21AB9716.seq Check: 8780 from: 1 to: 5884
pFN21AB9716 5884 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2760 3694 278
Size: 53 83 186 936 1224 934 2468
Fragments arranged by size:
2468 1224 936 934 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3947 5331 5599 1632
Size: 2315 1384 268 1917
Fragments arranged by size:
2315 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 2886 4783 5760 1203
Size: 1683 1897 977 1327
Fragments arranged by size:
1897 1683 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 2055 2142 2190 1166
Size: 889 87 48 4860
Fragments arranged by size:
4860 889 87 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2960 1184
Size: 1776 4108
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 5852
AlwNI CAG_nnn'CTG
Cuts at: 2713 3627 5199 2713
Size: 914 1572 3398
Fragments arranged by size:
3398 1572 914
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 3193 3419 3571 5680 1493
Size: 1700 226 152 2109 1697
Fragments arranged by size:
2109 1700 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 3138 3209 1870
Size: 73 126 1069 71 4545
Fragments arranged by size:
4545 1069 126 73 71
BamHI G'GATC_C
Cuts at: 2549 2568 3214 3581 2549
Size: 19 646 367 4852
Fragments arranged by size:
4852 646 367 19
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3016 3205 4660 618
Size: 325 217 627 1229 189 1455 1842
Fragments arranged by size:
1842 1455 1229 627 325 217 189
BanII G_rGCy'C
Cuts at: 1391 2633 3146 3203 1391
Size: 1242 513 57 4072
Fragments arranged by size:
4072 1242 513 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2752 928
Size: 971 853 4060
Fragments arranged by size:
4060 971 853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4075 4109 1903
Size: 34 2138 34 3678
Fragments arranged by size:
3678 2138 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4495 136
Size: 107 122 71 631 605 2823 1525
Fragments arranged by size:
2823 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5879 5879
Size: 5884
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2391 2970 3945 5101 1790
Size: 601 579 975 1156 2573
Fragments arranged by size:
2573 1156 975 601 579
BmgBI CAC'GTC
Cuts at: 1318 2107 1318
Size: 789 5095
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2537 3052 3615
Size: 416 53 83 399 24 1120 515 563
Cuts at: 3615 4573 442
Size: 958 1753
Fragments arranged by size:
1753 1120 958 563 515 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2369 2958 2369
Size: 589 5295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4544 1239
Size: 3305 2579
Bpu10I CC'TnA_GC
Cuts at: 2871 2871
Size: 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4009 4874 5172 4009
Size: 865 298 4721
Fragments arranged by size:
4721 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2216 2340 4547 882
Size: 617 717 124 2207 2219
Fragments arranged by size:
2219 2207 717 617 124
BsaAI yAC'GTr
Cuts at: 493 1204 2965 3675 493
Size: 711 1761 710 2702
Fragments arranged by size:
2702 1761 711 710
BsaBI GATnn'nnATC
Cuts at: 5878 5878
Size: 5884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2276 2757 3691 4073
Size: 53 83 186 936 743 481 934 382
Cuts at: 4073 4708 275
Size: 635 1451
Fragments arranged by size:
1451 936 934 743 635 481 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2418 4317 4715 4989 5136 5547 2418
Size: 1899 398 274 147 411 2755
Fragments arranged by size:
2755 1899 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2048 2789 2819 2981
Size: 30 165 30 615 30 741 30 162
Cuts at: 2981 3011 1178
Size: 30 4051
Fragments arranged by size:
4051 741 615 165 162 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5884
BseYI C'CCAG_C
Cuts at: 2390 2462 2501 2703 2812 2875 5087 5583 2390
Size: 72 39 202 109 63 2212 496 2691
Fragments arranged by size:
2691 2212 496 202 109 72 63 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5311
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2092 3334 4095 5123 1540
Size: 275 186 91 1242 761 1028 2301
Fragments arranged by size:
2301 1242 1028 761 275 186 91
BsiHKAI G_wGCw'C
Cuts at: 2778 2939 3945 4030 5101 2778
Size: 161 1006 85 1071 3561
Fragments arranged by size:
3561 1071 1006 161 85
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
BsmBI CGTCTCn'nnnn_
Cuts at: 2401 2401
Size: 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 2633 2778 2939 2970 3146 3203
Size: 399 601 242 145 161 31 176 57
Cuts at: 3203 3945 4030 5101 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 399 242 176 161
145 85 57 31
BspEI T'CCGG_A
Cuts at: 2418 5547 2418
Size: 3129 2755
BspHI T'CATG_A
Cuts at: 3773 5503 3773
Size: 1730 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2202 3239 844
Size: 1358 1037 3489
Fragments arranged by size:
3489 1358 1037
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4379 4553 65
Size: 1183 3131 174 1396
Fragments arranged by size:
3131 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 2091 2225 2349 4528 4715 1700
Size: 110 236 45 134 124 2179 187 2869
Fragments arranged by size:
2869 2179 236 187 134 124 110 45
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 3944 4956 3944
Size: 1012 4872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5884
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 2951 1184
Size: 1767 4117
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2287
Size: 5884
BstYI r'GATC_y
Cuts at: 1972 2549 2568 3214 3581 3966 3983 5435 5521
Size: 577 19 646 367 385 17 1452 86
Cuts at: 5521 5533 5879 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 646 577 385 367 346 86
19 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2185 513
Size: 552 939 181 4212
Fragments arranged by size:
4212 939 552 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2054 487
Size: 683 56 828 4317
Fragments arranged by size:
4317 828 683 56
BtsI GCAGTG_nn'
Cuts at: 2014 3400 4194 4214 2014
Size: 1386 794 20 3684
Fragments arranged by size:
3684 1386 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2358 2485
Size: 54 1425 50 275 18 259 269 127
Cuts at: 2485 2977 3331 4220 5849 8
Size: 492 354 889 1629 43
Fragments arranged by size:
1629 1425 889 492 354 275 269 259
127 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2089 3331 1537
Size: 275 277 1242 4090
Fragments arranged by size:
4090 1242 277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2404 2594 3814 1353
Size: 1051 190 1220 3423
Fragments arranged by size:
3423 1220 1051 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3136 4471 4845 4991 387
Size: 1064 138 1547 1335 374 146 1280
Fragments arranged by size:
1547 1335 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3947 4544 5331 5599 1239
Size: 393 2315 597 787 268 1524
Fragments arranged by size:
2315 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 5884
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3132 3132
Size: 5884
FseI GG_CCGG'CC
Cuts at: 2095 2095
Size: 5884
FspI TGC'GCA
Cuts at: 4390 4390
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 2532 5031 1678
Size: 854 2499 2531
Fragments arranged by size:
2531 2499 854
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 4525 4557 4599
Size: 32 135 32 446 32 2475 32 42
Cuts at: 4599 4631 1373
Size: 32 2626
Fragments arranged by size:
2626 2475 446 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3228 3474 669
Size: 988 281 1290 246 3079
Fragments arranged by size:
3079 1290 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2718 748
Size: 1970 3914
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4998 5182 1305
Size: 3693 184 2007
Fragments arranged by size:
3693 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2487 2979 10
Size: 54 1425 343 655 492 2915
Fragments arranged by size:
2915 1425 655 492 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2055 2142 2190 3842 4201 4360
Size: 648 843 46 87 48 1652 359 159
Cuts at: 4360 518
Size: 2042
Fragments arranged by size:
2042 1652 843 648 359 159 87 48
46
MspA1I CmG'CkG
Cuts at: 1606 2173 2713 3158 3167 3300 3977 5125 5370
Size: 567 540 445 9 133 677 1148 245
Cuts at: 5370 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 567 540 445 245 133
9
NaeI GCC'GGC
Cuts at: 2093 2093
Size: 5884
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4393
Fragments arranged by size:
4393 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NgoMIV G'CCGG_C
Cuts at: 2091 2091
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 3242 4787 1132
Size: 97 2013 1545 2229
Fragments arranged by size:
2229 2013 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2825 1348
Size: 1477 4407
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5884
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PmlI CAC'GTG
Cuts at: 2965 2965
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 3953 3985 5466 5498 2789
Size: 32 1132 32 1481 32 3175
Fragments arranged by size:
3175 1481 1132 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PstI C_TGCA'G
Cuts at: 830 3236 830
Size: 2406 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
PvuII CAG'CTG
Cuts at: 2173 2713 2173
Size: 540 5344
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 2804 4132 5844 2804
Size: 1328 1712 2844
Fragments arranged by size:
2844 1712 1328
SfcI C'TryA_G
Cuts at: 826 1045 3232 4367 5048 5239 826
Size: 219 2187 1135 681 191 1471
Fragments arranged by size:
2187 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 3140 3211 1872
Size: 1268 71 4545
Fragments arranged by size:
4545 1268 71
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3988 4889 5151 820
Size: 197 926 2045 901 262 1553
Fragments arranged by size:
2045 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5884
StyI C'CwwG_G
Cuts at: 513 1065 2004 3323 513
Size: 552 939 1319 3074
Fragments arranged by size:
3074 1319 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2460 2717 3911 3928 4081 4266 4711
Size: 1255 880 257 1194 17 153 185 445
Cuts at: 4711 325
Size: 1498
Fragments arranged by size:
1498 1255 1194 880 445 257 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2802 3060 4130
Size: 274 80 33 51 1443 825 258 1070
Cuts at: 4130 5842 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1070 825 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2482 2514 5844 5876 2482
Size: 32 3330 32 2490
Fragments arranged by size:
3330 2490 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 2542 2872 3001 2266
Size: 276 330 129 5149
Fragments arranged by size:
5149 330 276 129
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 3138 3209 1870
Size: 1268 71 4545
Fragments arranged by size:
4545 1268 71
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2758 3692 276
Size: 53 83 186 936 1224 934 2468
Fragments arranged by size:
2468 1224 936 934 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV
FspAI KasI NarI NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI