Restriction map for
pFN21AB9704
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI HaeII Hin1I HincII HindII
HindIII HpaI KasI MaeII MamI
MfeI MflI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 671 GGTACC G^GTACC
AlwI 390 GATCC GGATC(4/5)
AlwNI 279 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 212 GGGCCC GGGCC^C
Asp718I 671 GGTACC G^GTACC
AviII 578 TGCGCA TGC^GCA
BalI 275 TGGCCA TGG^CCA
BbsI 624 GAAGAC GAAGAC(2/6)
BcgI 830 CGAGCGGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 19 CTAG C^TAG
BpuAI 624 GAAGAC GAAGAC(2/6)
BseRI 298 GAGGAG GAGGAG(10/8)
BsmFI 611 GGGAC GGGAC(10/14)
BspMI 270 GCAGGT ACCTGC(4/8)
BsrDI 631 CATTGC GCAATG(2/0)
BssSI 382 CACGAG CACGAG(-5/-1)
BstAPI 701 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 966 GGTAACC G^GT[ACGT]ACC
BstPI 966 GGTAACC G^GT[ACGT]ACC
DpnII 390 GATC ^GATC
EagI 543 CGGCCG C^GGCCG
EarI 223 GAAGAG CTCTTC(1/4)
EclXI 543 CGGCCG C^GGCCG
Eco52I 543 CGGCCG C^GGCCG
EcoO65I 966 GGTAACC G^GT[ACGT]ACC
EcoT14I 473 CCAAGG C^C[AT][AT]GG
FspI 578 TGCGCA TGC^GCA
KpnI 671 GGTACC GGTAC^C
Ksp632I 223 GAAGAG CTCTTC(1/4)
KspI 788 CCGCGG CCGC^GG
MaeI 19 CTAG C^TAG
MaeIII 967 GTAAC ^GT[ACGT]AC
MboI 390 GATC ^GATC
MluNI 275 TGGCCA TGG^CCA
MscI 275 TGGCCA TGG^CCA
MseI 761 TTAA T^TAA
MslI 912 CACCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 390 GATC ^GATC
NotI 542 GCGGCCGC GC^GGCCGC
NspI 836 GCATGC [GA]CATG^[CT]
PaeR7I 508 CTCGAG C^TCGAG
PpuMI 680 AGGACCC [GA]G^G[AT]CC[CT]
PstI 493 CTGCAG CTGCA^G
PvuII 241 CAGCTG CAG^CTG
SacII 788 CCGCGG CCGC^GG
Sau3AI 390 GATC ^GATC
SmaI 355 CCCGGG CCC^GGG
SphI 836 GCATGC GCATG^C
StyI 473 CCAAGG C^C[AT][AT]GG
TfiI 61 GATTC G^A[AT]TC
Tru9I 761 TTAA T^TAA
XcmI 598 CCACGCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 508 CTCGAG C^TCGAG
XmaCI 355 CCCGGG C^CCGGG
XmaI 355 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 570 GTAC GT^AC
672 GTAC
AvaI 355 CCCGGG C^[CT]CG[GA]G
508 CTCGAG
AvaII 681 GGACC G^G[AT]CC
826 GGACC
BanI 671 GGTACC G^G[CT][GA]CC
949 GGCACC
BanII 212 GGGCCC G[GA]GC[CT]^C
853 GAGCCC
BmyI 212 GGGCCC G[AGT]GC[ACT]^C
853 GAGCCC
BsiEI 543 CGGCCG CG[GA][CT]^CG
780 CGACCG
BsmBI 289 GAGACG CGTCTC(1/5)
301 GAGACG
BsoBI 355 CCCGGG C^[CT]CG[GA]G
508 CTCGAG
Bsp1286I 212 GGGCCC G[AGT]GC[ACT]^C
853 GAGCCC
BsrI 714 ACTGG ACTGG(1/-1)
755 ACTGG
BstF5I 450 CATCC GGATG(2/0)
957 CATCC
BstXI 77 CCATACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
816 CCAGGCCATTGG
FokI 450 CATCC GGATG(9/13)
957 CATCC
MboII 223 GAAGA GAAGA(8/7)
624 GAAGA
RsaI 570 GTAC GT^AC
672 GTAC
SfcI 493 CTGCAG C^T[GA][CT]AG
676 CTACAG
SmlI 454 CTTGAG C^T[CT][GA]AG
508 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BglI BpmI BsaJI BsiYI BslI
BsmAI BsrBI BsrFI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI Sau96I ScrFI TaqI
TseI TthHB8I