(Circular) (Six-base) MAPSORT of: pFN21AB9704.seq Check: 8580 from: 1 to: 5701 pFN21AB9704 5701 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2266 2266 Size: 5701 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3511 278 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3044 1937 Size: 1107 4594 Acc65I G'GTAC_C Cuts at: 2676 3022 2676 Size: 346 5355 AclI AA'CG_TT Cuts at: 1563 3828 4201 1563 Size: 2265 373 3063 Fragments arranged by size: 3063 2265 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3764 5148 5416 1632 Size: 2132 1384 268 1917 Fragments arranged by size: 2132 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5504 AflIII A'CryG_T Cuts at: 1203 4600 5577 1203 Size: 3397 977 1327 Fragments arranged by size: 3397 1327 977 AgeI A'CCGG_T Cuts at: 4532 4532 Size: 5701 AhdI GACnn_n'nnGTC Cuts at: 4430 4430 Size: 5701 AleI CACnn'nnGTG Cuts at: 1166 2921 1166 Size: 1755 3946 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5701 AlwNI CAG_nnn'CTG Cuts at: 2289 3444 5016 2289 Size: 1155 1572 2974 Fragments arranged by size: 2974 1572 1155 ApaI G_GGCC'C Cuts at: 2221 2221 Size: 5701 ApaLI G'TGCA_C Cuts at: 3758 4914 3758 Size: 1156 4545 ApoI r'AATT_y Cuts at: 1493 3010 3236 3388 5497 1493 Size: 1517 226 152 2109 1697 Fragments arranged by size: 2109 1697 1517 226 152 AseI AT'TA_AT Cuts at: 160 1032 4255 160 Size: 872 3223 1606 Fragments arranged by size: 3223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 AvaI C'yCGr_G Cuts at: 1870 1943 2360 2513 3026 1870 Size: 73 417 153 513 4545 Fragments arranged by size: 4545 513 417 153 73 BamHI G'GATC_C Cuts at: 3031 3398 3031 Size: 367 5334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2676 2954 3022 4477 618 Size: 325 217 627 889 278 68 1455 1842 Fragments arranged by size: 1842 1455 889 627 325 278 217 68 BanII G_rGCy'C Cuts at: 1391 2221 2862 3020 1391 Size: 830 641 158 4072 Fragments arranged by size: 4072 830 641 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2636 928 Size: 971 737 3993 Fragments arranged by size: 3993 971 737 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2824 2858 3892 3926 1903 Size: 34 887 34 1034 34 3678 Fragments arranged by size: 3678 1034 887 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3593 4803 3593 Size: 1210 4491 BclI T'GATC_A Cuts at: 1548 1548 Size: 5701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2363 2559 2798 Size: 107 122 71 631 605 691 196 239 Cuts at: 2798 4312 136 Size: 1514 1525 Fragments arranged by size: 1525 1514 691 631 605 239 196 122 107 71 BglII A'GATC_T Cuts at: 5696 5696 Size: 5701 BlpI GC'TnA_GC Cuts at: 3118 3118 Size: 5701 Bme1580I G_kGCm'C Cuts at: 1790 2221 3762 4918 1790 Size: 431 1541 1156 2573 Fragments arranged by size: 2573 1541 1156 431 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5701 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3432 4390 442 Size: 416 53 83 399 24 2015 958 1753 Fragments arranged by size: 2015 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5701 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2197 2321 2798 2962 4361 1239 Size: 958 124 477 164 1399 2579 Fragments arranged by size: 2579 1399 958 477 164 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2480 3826 4691 4989 2480 Size: 1346 865 298 3192 Fragments arranged by size: 3192 1346 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4364 882 Size: 617 2865 2219 Fragments arranged by size: 2865 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3492 493 Size: 711 2288 2702 Fragments arranged by size: 2702 2288 711 BsaBI GATnn'nnATC Cuts at: 5695 5695 Size: 5701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3508 3890 4525 275 Size: 53 83 186 936 1975 382 635 1451 Fragments arranged by size: 1975 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4134 4532 4806 4953 5364 4134 Size: 398 274 147 411 4471 Fragments arranged by size: 4471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2063 2093 1178 Size: 30 165 30 660 30 4786 Fragments arranged by size: 4786 660 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 1186 Size: 1132 4569 BseYI C'CCAG_C Cuts at: 2326 2702 4904 5400 2326 Size: 376 2202 496 2627 Fragments arranged by size: 2627 2202 496 376 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5128 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2551 2788 3151 3912 4940 1540 Size: 275 186 550 237 363 761 1028 2301 Fragments arranged by size: 2301 1028 761 550 363 275 237 186 BsiHKAI G_wGCw'C Cuts at: 3762 3847 4918 3762 Size: 85 1071 4545 Fragments arranged by size: 4545 1071 85 BsmI GAATG_Cn' Cuts at: 3212 3305 3212 Size: 93 5608 BsmBI CGTCTCn'nnnn_ Cuts at: 2288 2300 2288 Size: 12 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2221 2862 3020 3762 3847 4918 1391 Size: 399 431 641 158 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 641 431 399 158 85 BspEI T'CCGG_A Cuts at: 5364 5364 Size: 5701 BspHI T'CATG_A Cuts at: 3590 5320 3590 Size: 1730 3971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2266 3056 844 Size: 1422 790 3489 Fragments arranged by size: 3489 1422 790 BsrBI CCG'CTC Cuts at: 1394 1823 2016 2079 2323 2650 2838 3588 1394 Size: 429 193 63 244 327 188 750 3507 Fragments arranged by size: 3507 750 429 327 244 193 188 63 BsrDI GCAATG_nn' Cuts at: 65 1248 2635 4196 4370 65 Size: 1183 1387 1561 174 1396 Fragments arranged by size: 1561 1396 1387 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2732 2772 2951 4345 4532 1700 Size: 110 922 40 179 1394 187 2869 Fragments arranged by size: 2869 1394 922 187 179 110 40 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5701 BssHII G'CGCG_C Cuts at: 1925 2401 2403 2405 2407 2941 1925 Size: 476 2 2 2 534 4685 Fragments arranged by size: 4685 534 476 2 2 2 BssSI C'ACGA_G Cuts at: 2387 3761 4773 2387 Size: 1374 1012 3315 Fragments arranged by size: 3315 1374 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2712 794 Size: 1918 3783 BstBI TT'CG_AA Cuts at: 4505 4505 Size: 5701 BstEII G'GTnAC_C Cuts at: 1184 2971 1184 Size: 1787 3914 BstXI CCAn_nnnn'nTGG Cuts at: 2089 2828 2089 Size: 739 4962 BstYI r'GATC_y Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696 Size: 1059 367 385 17 1452 86 12 346 Cuts at: 5696 1972 Size: 1977 Fragments arranged by size: 1977 1452 1059 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2352 2412 2793 2898 513 Size: 552 939 348 60 381 105 3316 Fragments arranged by size: 3316 939 552 381 348 105 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4962 Fragments arranged by size: 4962 683 56 BtsI GCAGTG_nn' Cuts at: 3217 4011 4031 3217 Size: 794 20 4887 Fragments arranged by size: 4887 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5701 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5666 DraI TTT'AAA Cuts at: 3005 3005 Size: 5701 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4708 809 Size: 507 3392 1802 Fragments arranged by size: 3392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2548 2799 Size: 54 1425 50 275 18 450 268 251 Cuts at: 2799 2853 2904 3148 4037 5666 8 Size: 54 51 244 889 1629 43 Fragments arranged by size: 1629 1425 889 450 275 268 251 244 54 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2548 3148 1537 Size: 275 736 600 4090 Fragments arranged by size: 4090 736 600 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2223 3631 1353 Size: 870 1408 3423 Fragments arranged by size: 3423 1408 870 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2199 4288 4662 4808 387 Size: 1064 138 610 2089 374 146 1280 Fragments arranged by size: 2089 1280 1064 610 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2197 2321 2798 2962 3764 4361 5148 Size: 393 565 124 477 164 802 597 787 Cuts at: 5148 5416 1239 Size: 268 1524 Fragments arranged by size: 1524 802 787 597 565 477 393 268 164 124 EcoO109I rG'GnC_Cy Cuts at: 2217 2218 2686 2217 Size: 1 468 5232 Fragments arranged by size: 5232 468 1 EcoRI G'AATT_C Cuts at: 3010 3388 3010 Size: 378 5323 FspI TGC'GCA Cuts at: 2585 4207 2585 Size: 1622 4079 HaeII r_GCGC'y Cuts at: 1678 4848 1678 Size: 3170 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2879 2911 4342 4374 4416 Size: 32 135 32 1307 32 1431 32 42 Cuts at: 4416 4448 1373 Size: 32 2626 Fragments arranged by size: 2626 1431 1307 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3045 3291 669 Size: 988 281 1107 246 3079 Fragments arranged by size: 3079 1107 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5701 HpaI GTT'AAC Cuts at: 3291 3291 Size: 5701 KpnI G_GTAC'C Cuts at: 2680 3026 2680 Size: 346 5355 MfeI C'AATT_G Cuts at: 3300 3300 Size: 5701 MluI A'CGCG_T Cuts at: 5577 5577 Size: 5701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4815 4999 1305 Size: 3510 184 2007 Fragments arranged by size: 3510 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 10 Size: 54 1425 343 450 3429 Fragments arranged by size: 3429 1425 450 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2921 3659 4018 4177 518 Size: 648 1755 738 359 159 2042 Fragments arranged by size: 2042 1755 738 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2148 2248 2599 2795 3117 3794 4942 5187 Size: 542 100 351 196 322 677 1148 245 Cuts at: 5187 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 542 351 322 245 196 100 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4210 Fragments arranged by size: 4210 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5701 NotI GC'GGCC_GC Cuts at: 2548 3148 2548 Size: 600 5101 NspI r_CATG'y Cuts at: 1132 1229 2845 3059 4604 1132 Size: 97 1616 214 1545 2229 Fragments arranged by size: 2229 1616 1545 214 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5701 PciI A'CATG_T Cuts at: 4600 4600 Size: 5701 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5701 PmeI GTTT'AAAC Cuts at: 3005 3005 Size: 5701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3770 3802 5283 5315 3770 Size: 32 1481 32 4156 Fragments arranged by size: 4156 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2686 2686 Size: 5701 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5701 PsiI TTA'TAA Cuts at: 3271 3271 Size: 5701 PspOMI G'GGCC_C Cuts at: 2217 2217 Size: 5701 PspXI vC'TCGA_Gb Cuts at: 2513 2513 Size: 5701 PstI C_TGCA'G Cuts at: 830 2502 3053 830 Size: 1672 551 3478 Fragments arranged by size: 3478 1672 551 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5701 PvuII CAG'CTG Cuts at: 2248 2248 Size: 5701 SacII CC_GC'GG Cuts at: 2796 2796 Size: 5701 SalI G'TCGA_C Cuts at: 1936 3043 1936 Size: 1107 4594 SbfI CC_TGCA'GG Cuts at: 3053 3053 Size: 5701 ScaI AGT'ACT Cuts at: 3949 5661 3949 Size: 1712 3989 SfcI C'TryA_G Cuts at: 826 1045 2498 2681 3049 4184 4865 5056 826 Size: 219 1453 183 368 1135 681 191 1471 Fragments arranged by size: 1471 1453 1135 681 368 219 191 183 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5701 SmaI CCC'GGG Cuts at: 1872 2362 3028 1872 Size: 490 666 4545 Fragments arranged by size: 4545 666 490 SmlI C'TyrA_G Cuts at: 820 1017 1943 2459 2513 3805 4706 4968 820 Size: 197 926 516 54 1292 901 262 1553 Fragments arranged by size: 1553 1292 926 901 516 262 197 54 SnaBI TAC'GTA Cuts at: 493 3492 493 Size: 2999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5701 SphI G_CATG'C Cuts at: 1132 2845 3059 1132 Size: 1713 214 3774 Fragments arranged by size: 3774 1713 214 SspI AAT'ATT Cuts at: 5 52 3625 5 Size: 47 3573 2081 Fragments arranged by size: 3573 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5701 StyI C'CwwG_G Cuts at: 513 1065 2004 2478 3140 513 Size: 552 939 474 662 3074 Fragments arranged by size: 3074 939 662 552 474 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2848 3728 3745 3898 4083 4528 325 Size: 1255 1268 880 17 153 185 445 1498 Fragments arranged by size: 1498 1268 1255 880 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3947 5659 96 Size: 274 80 33 51 1443 1970 1712 138 Fragments arranged by size: 1970 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2063 2095 5661 5693 2063 Size: 32 3566 32 2071 Fragments arranged by size: 3566 2071 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5701 XbaI T'CTAG_A Cuts at: 3037 3037 Size: 5701 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2610 2610 Size: 5701 XhoI C'TCGA_G Cuts at: 1943 2513 1943 Size: 570 5131 XmaI C'CCGG_G Cuts at: 1870 2360 3026 1870 Size: 490 666 4545 Fragments arranged by size: 4545 666 490 XmnI GAAnn'nnTTC Cuts at: 3830 3830 Size: 5701 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3509 276 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI