(Circular) (Six-base) MAPSORT of: pFN21AB9702.seq Check: 3001 from: 1 to: 5686 pFN21AB9702 5686 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2244 2244 Size: 5686 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3029 1937 Size: 1092 4594 Acc65I G'GTAC_C Cuts at: 2679 3007 2679 Size: 328 5358 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3749 5133 5401 1632 Size: 2117 1384 268 1917 Fragments arranged by size: 2117 1917 1384 268 AfeI AGC'GCT Cuts at: 2760 2760 Size: 5686 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 4585 5562 1203 Size: 3382 977 1327 Fragments arranged by size: 3382 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5686 AlwNI CAG_nnn'CTG Cuts at: 2145 2372 2803 3429 5001 2145 Size: 227 431 626 1572 2830 Fragments arranged by size: 2830 1572 626 431 227 ApaLI G'TGCA_C Cuts at: 2337 3743 4899 2337 Size: 1406 1156 3124 Fragments arranged by size: 3124 1406 1156 ApoI r'AATT_y Cuts at: 1493 2995 3221 3373 5482 1493 Size: 1502 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2243 2982 3011 1870 Size: 73 147 153 739 29 4545 Fragments arranged by size: 4545 739 153 147 73 29 BamHI G'GATC_C Cuts at: 3016 3383 3016 Size: 367 5319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2679 2887 2918 3007 4462 Size: 325 217 627 892 208 31 89 1455 Cuts at: 4462 618 Size: 1842 Fragments arranged by size: 1842 1455 892 627 325 217 208 89 31 BanII G_rGCy'C Cuts at: 1391 2637 3005 1391 Size: 1246 368 4072 Fragments arranged by size: 4072 1246 368 BbeI G_GCGC'C Cuts at: 2922 2922 Size: 5686 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2308 2342 2617 2651 3877 3911 1903 Size: 34 371 34 275 34 1226 34 3678 Fragments arranged by size: 3678 1226 371 275 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 2295 1548 Size: 747 4939 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2493 5681 2493 Size: 3188 2498 BlpI GC'TnA_GC Cuts at: 3103 3103 Size: 5686 Bme1580I G_kGCm'C Cuts at: 1790 2341 2892 3747 4903 1790 Size: 551 551 855 1156 2573 Fragments arranged by size: 2573 1156 855 551 551 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2260 3417 4375 Size: 416 53 83 399 24 843 1157 958 Cuts at: 4375 442 Size: 1753 Fragments arranged by size: 1753 1157 958 843 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2148 2482 2488 2603 2747 2858 2897 Size: 893 16 334 6 115 144 111 39 Cuts at: 2897 4346 1239 Size: 1449 2579 Fragments arranged by size: 2579 1449 893 334 144 115 111 39 16 6 Bpu10I CC'TnA_GC Cuts at: 2373 2612 2373 Size: 239 5447 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2872 4349 882 Size: 617 1373 1477 2219 Fragments arranged by size: 2219 1477 1373 617 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2919 2958 3493 3875 Size: 53 83 186 936 1386 39 535 382 Cuts at: 3875 4510 275 Size: 635 1451 Fragments arranged by size: 1451 1386 936 635 535 382 186 83 53 39 BsaWI w'CCGG_w Cuts at: 4119 4517 4791 4938 5349 4119 Size: 398 274 147 411 4456 Fragments arranged by size: 4456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5461 Fragments arranged by size: 5461 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2275 2492 1186 Size: 1089 217 4380 Fragments arranged by size: 4380 1089 217 BseYI C'CCAG_C Cuts at: 2852 4889 5385 2852 Size: 2037 496 3153 Fragments arranged by size: 3153 2037 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2639 1163 Size: 573 903 4210 Fragments arranged by size: 4210 903 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2488 2718 3136 3897 4925 1540 Size: 275 186 487 230 418 761 1028 2301 Fragments arranged by size: 2301 1028 761 487 418 275 230 186 BsiHKAI G_wGCw'C Cuts at: 2341 2637 3747 3832 4903 2341 Size: 296 1110 85 1071 3124 Fragments arranged by size: 3124 1110 1071 296 85 BsmI GAATG_Cn' Cuts at: 3197 3290 3197 Size: 93 5593 BsmBI CGTCTCn'nnnn_ Cuts at: 2177 2177 Size: 5686 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2341 2637 2892 3005 3747 3832 4903 Size: 399 551 296 255 113 742 85 1071 Cuts at: 4903 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 551 399 296 255 113 85 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 3575 5305 3575 Size: 1730 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2656 3041 844 Size: 1400 412 385 3489 Fragments arranged by size: 3489 1400 412 385 BsrBI CCG'CTC Cuts at: 1394 1823 2599 3573 1394 Size: 429 776 974 3507 Fragments arranged by size: 3507 974 776 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4181 4355 65 Size: 1183 2933 174 1396 Fragments arranged by size: 2933 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2362 2954 4330 4517 1700 Size: 110 552 592 1376 187 2869 Fragments arranged by size: 2869 1376 592 552 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5686 BssHII G'CGCG_C Cuts at: 1925 2546 2856 1925 Size: 621 310 4755 Fragments arranged by size: 4755 621 310 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 2145 794 Size: 1351 4335 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5686 BstYI r'GATC_y Cuts at: 1972 2493 3016 3383 3768 3785 5237 5323 5335 Size: 521 523 367 385 17 1452 86 12 Cuts at: 5335 5681 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 523 521 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2195 2403 2845 2905 513 Size: 552 939 191 208 442 60 3294 Fragments arranged by size: 3294 939 552 442 208 191 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4947 Fragments arranged by size: 4947 683 56 BtsI GCAGTG_nn' Cuts at: 3202 3996 4016 3202 Size: 794 20 4872 Fragments arranged by size: 4872 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2666 4693 809 Size: 507 1350 2027 1802 Fragments arranged by size: 2027 1802 1350 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2394 2715 2952 Size: 54 1425 50 275 18 564 321 237 Cuts at: 2952 3133 4022 5651 8 Size: 181 889 1629 43 Fragments arranged by size: 1629 1425 889 564 321 275 237 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2715 3133 1537 Size: 275 903 418 4090 Fragments arranged by size: 4090 903 418 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2155 3616 1353 Size: 802 1461 3423 Fragments arranged by size: 3423 1461 802 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2753 4273 4647 4793 387 Size: 1064 138 1164 1520 374 146 1280 Fragments arranged by size: 1520 1280 1164 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2635 2635 Size: 5686 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2148 2482 2488 2603 2747 2858 Size: 393 500 16 334 6 115 144 111 Cuts at: 2858 2897 3749 4346 5133 5401 1239 Size: 39 852 597 787 268 1524 Fragments arranged by size: 1524 852 787 597 500 393 334 268 144 115 111 39 16 6 EcoNI CCTnn'n_nnAGG Cuts at: 2072 2072 Size: 5686 EcoO109I rG'GnC_Cy Cuts at: 2080 2190 2080 Size: 110 5576 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 2762 2834 2922 4833 1678 Size: 1084 72 88 1911 2531 Fragments arranged by size: 2531 1911 1084 88 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4327 4359 4401 4433 1373 Size: 32 135 32 2755 32 42 32 2626 Fragments arranged by size: 2755 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KasI G'GCGC_C Cuts at: 2918 2918 Size: 5686 KpnI G_GTAC'C Cuts at: 2683 3011 2683 Size: 328 5358 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2706 2835 4800 4984 1305 Size: 1401 129 1965 184 2007 Fragments arranged by size: 2007 1965 1401 184 129 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3864 Fragments arranged by size: 3864 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3644 4003 4162 518 Size: 648 2478 359 159 2042 Fragments arranged by size: 2478 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2355 2405 2572 2581 2767 2847 2941 3102 Size: 749 50 167 9 186 80 94 161 Cuts at: 3102 3779 4927 5172 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 749 677 245 186 167 161 94 80 50 9 NaeI GCC'GGC Cuts at: 2364 2956 2364 Size: 592 5094 NarI GG'CG_CC Cuts at: 2919 2919 Size: 5686 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4195 Fragments arranged by size: 4195 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NgoMIV G'CCGG_C Cuts at: 2362 2954 2362 Size: 592 5094 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 3044 4589 1132 Size: 97 1815 1545 2229 Fragments arranged by size: 2229 1815 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5686 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 2201 2572 1108 Size: 1093 371 4222 Fragments arranged by size: 4222 1093 371 PfoI T'CCnGG_A Cuts at: 2164 2164 Size: 5686 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2190 2080 Size: 110 5576 PshAI GACnn'nnGTC Cuts at: 1613 2717 1613 Size: 1104 4582 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PstI C_TGCA'G Cuts at: 830 2241 3038 830 Size: 1411 797 3478 Fragments arranged by size: 3478 1411 797 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 PvuII CAG'CTG Cuts at: 2572 2581 2572 Size: 9 5677 SacI G_AGCT'C Cuts at: 2637 2637 Size: 5686 SacII CC_GC'GG Cuts at: 2406 2848 2406 Size: 442 5244 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SanDI GG'GwC_CC Cuts at: 2080 2190 2080 Size: 110 5576 SapI GCTCTTCn'nnn_ Cuts at: 2155 2155 Size: 5686 SbfI CC_TGCA'GG Cuts at: 3038 3038 Size: 5686 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SexAI A'CCwGG_T Cuts at: 2675 2675 Size: 5686 SfcI C'TryA_G Cuts at: 826 1045 2237 3034 4169 4850 5041 826 Size: 219 1192 797 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 797 681 219 191 SfoI GGC'GCC Cuts at: 2920 2920 Size: 5686 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 2245 3013 1872 Size: 373 768 4545 Fragments arranged by size: 4545 768 373 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 3044 1132 Size: 1912 3774 SrfI GCCC'GGGC Cuts at: 2245 2245 Size: 5686 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5686 StyI C'CwwG_G Cuts at: 513 1065 2004 2073 2397 3125 513 Size: 552 939 69 324 728 3074 Fragments arranged by size: 3074 939 728 552 324 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3713 3730 3883 4068 4513 325 Size: 1255 2133 17 153 185 445 1498 Fragments arranged by size: 2133 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3932 5644 96 Size: 274 80 33 51 1443 1955 1712 138 Fragments arranged by size: 1955 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5646 5678 5646 Size: 32 5654 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 2243 3011 1870 Size: 373 768 4545 Fragments arranged by size: 4545 768 373 XmnI GAAnn'nnTTC Cuts at: 3815 3815 Size: 5686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SfiI SwaI XcmI