Restriction map for
pFN21AB9700
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BanII BbeI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HaeII HhaI HinP1I
HinfI HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
MwoI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 852 GCGG CCGC(-3/-1)
AcyI 286 GGCGTC G[GA]^CG[CT]C
AflIII 736 ACATGT A^C[GA][CT]GT
AspHI 109 GTGCTC G[AT]GC[AT]^C
BamHI 33 GGATCC G^GATCC
BanI 374 GGCACC G^G[CT][GA]CC
BciVI 169 GTATCC GTATCC(6/5)
BcnI 174 CCCGG CC^[GC]GG
BglII 606 AGATCT A^GATCT
BmyI 109 GTGCTC G[AGT]GC[ACT]^C
BsaHI 286 GGCGTC G[GA]^CG[CT]C
BsaWI 788 ACCGGA [AT]^CCGG[AT]
BsiHKAI 109 GTGCTC G[AT]GC[AT]^C
Bsp1286I 109 GTGCTC G[AGT]GC[ACT]^C
BspHI 741 TCATGA T^CATGA
BspLU11I 736 ACATGT A^CATGT
BsrI 388 CCAGT ACTGG(1/-1)
Cfr13I 48 GGCCC G^G[ACGT]CC
DraI 481 TTTAAA TTT^AAA
DraII 47 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 358 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 157 CCATGG C^C[GA][CT]GG
EcoO109I 47 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 542 GAATTC G^AATTC
HgaI 287 GCGTC GACGC(5/10)
Hin1I 286 GGCGTC G[GA]^CG[CT]C
HincII 572 GTCAAC GT[CT]^[GA]AC
HindII 572 GTCAAC GT[CT]^[GA]AC
HindIII 672 AAGCTT A^AGCTT
HphI 805 GGTGA GGTGA(8/7)
MaeIII 449 GTCAC ^GT[ACGT]AC
NciI 174 CCCGG CC^[GC]GG
NcoI 157 CCATGG C^CATGG
NdeI 828 CATATG CA^TATG
NspI 736 ACATGT [GA]CATG^[CT]
RcaI 741 TCATGA T^CATGA
SapI 151 GAAGAGC GCTCTTC(1/4)
Sau96I 48 GGCCC G^G[ACGT]CC
SfaNI 277 GCATC GCATC(5/9)
SfcI 206 CTACAG C^T[GA][CT]AG
Tsp45I 449 GTCAC ^GT[GC]AC
XbaI 138 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 501 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
669 GAAAAGCTTC
BbvI 810 GCAGC GCAGC(8/12)
429 GCTGC
BfaI 97 CTAG C^TAG
139 CTAG
BsmAI 82 GAGAC GTCTC(1/5)
252 GTCTC
BstNI 12 CCAGG CC^[AT]GG
416 CCTGG
BstYI 33 GGATCC [GA]^GATC[CT]
606 AGATCT
EarI 151 GAAGAG CTCTTC(1/4)
553 GAAGAG
EcoRII 12 CCAGG ^CC[AT]GG
416 CCTGG
EcoT14I 157 CCATGG C^C[AT][AT]GG
633 CCAAGG
Fnu4HI 429 GCTGC GC^[ACGT]GC
810 GCAGC
HaeIII 48 GGCC GG^CC
303 GGCC
ItaI 429 GCTGC GC^[ACGT]GC
810 GCAGC
Ksp632I 151 GAAGAG CTCTTC(1/4)
553 GAAGAG
MaeI 97 CTAG C^TAG
139 CTAG
MflI 33 GGATCC [GA]^GATC[CT]
606 AGATCT
MnlI 51 CCTC CCTC(7/6)
588 GAGG
MvaI 12 CCAGG CC^[AT]GG
416 CCTGG
NlaIV 33 GGATCC GG[ACGT]^[ACGT]CC
374 GGCACC
StyI 157 CCATGG C^C[AT][AT]GG
633 CCAAGG
TseI 429 GCTGC G^C[AT]GC
810 GCAGC
XhoII 33 GGATCC [GA]^GATC[CT]
606 AGATCT
XmnI 501 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
669 GAAAAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AcsI AluI AlwI ApoI BsaJI
BssKI Cac8I DdeI DpnII HapII
HpaII MboI MboII MseI MslI
MspI NdeII NlaIII Sau3AI ScrFI
Tru9I Tsp509I TspRI