Restriction map for
pFN21AB9696
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I MaeI
MamI MfeI MluI MroI MseI
MslI MunI NcoI NdeI NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 478 GTCTAC GT^[AC][GT]AC
AvaII 455 GGACC G^G[AT]CC
BalI 752 TGGCCA TGG^CCA
BbrPI 148 CACGTG CAC^GTG
BglII 596 AGATCT A^GATCT
BsaAI 148 CACGTG [CT]AC^GT[GA]
BsiEI 422 CGGCCG CG[GA][CT]^CG
BsmFI 731 GTCCC GGGAC(10/14)
BsmI 271 GCATTC GAATGC(1/-1)
BspMI 588 GCAGGT ACCTGC(4/8)
BsrI 212 ACTGG ACTGG(1/-1)
BstXI 743 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 596 AGATCT [GA]^GATC[CT]
DpnII 597 GATC ^GATC
DsaI 239 CCGCGG C^C[GA][CT]GG
EagI 422 CGGCCG C^GGCCG
EclXI 422 CGGCCG C^GGCCG
Eco52I 422 CGGCCG C^GGCCG
HgaI 390 GACGC GACGC(5/10)
HphI 591 GGTGA GGTGA(8/7)
KspI 239 CCGCGG CCGC^GG
MaeII 149 ACGT A^CGT
MaeIII 530 GTTAC ^GT[ACGT]AC
MboI 597 GATC ^GATC
MflI 596 AGATCT [GA]^GATC[CT]
MluNI 752 TGGCCA TGG^CCA
MscI 752 TGGCCA TGG^CCA
NdeII 597 GATC ^GATC
PflMI 492 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 450 GACTC GAGTC(4/5)
PmaCI 148 CACGTG CAC^GTG
PmlI 148 CACGTG CAC^GTG
SacII 239 CCGCGG CCGC^GG
Sau3AI 597 GATC ^GATC
SfaNI 405 GATGC GCATC(5/9)
TfiI 28 GATTC G^A[AT]TC
TspRI 579 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 492 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 62 CCAGCCTCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 596 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BglI 178 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
309 GCCCCCTGGGC
BseRI 759 CTCCTC GAGGAG(10/8)
781 GAGGAG
BstF5I 713 CATCC GGATG(2/0)
404 GGATG
EcoT14I 203 CCAAGG C^C[AT][AT]GG
458 CCAAGG
FokI 713 CATCC GGATG(9/13)
404 GGATG
HinfI 28 GATTC G^A[ACGT]TC
450 GACTC
MboII 594 GAAGA GAAGA(8/7)
642 GAAGA
MspA1I 239 CCGCGG C[AC]G^C[GT]G
441 CAGCGG
NaeI 156 GCCGGC GCC^GGC
303 GCCGGC
NgoMIV 156 GCCGGC G^CCGGC
303 GCCGGC
StyI 203 CCAAGG C^C[AT][AT]GG
458 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI BanI BanII BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsiYI BslI BsoBI Bsp1286I
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DraII EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI KasI MnlI MspI
MvaI MvnI MwoI NarI NciI
NlaIV RsaI Sau96I ScrFI SfoI
SmaI TseI XmaCI XmaI