Restriction map for
pFN21AB9696
(Linear) (Six-base) MAPSORT of: KIBB9696.seq Check: 6875 from: 1 to: 795
KIBB9696.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 579 795
Size: 579 216
AccI GT'mk_AC
Cuts at: 0 479 795
Size: 479 316
ApaI G_GGCC'C
Cuts at: 0 79 238 281 320 355 795
Size: 79 159 43 39 35 440
Fragments arranged by size:
440 159 79 43 39 35
AvaI C'yCGr_G
Cuts at: 0 231 324 346 354 795
Size: 231 93 22 8 441
Fragments arranged by size:
441 231 93 22 8
BanI G'GyrC_C
Cuts at: 0 111 186 300 306 795
Size: 111 75 114 6 489
Fragments arranged by size:
489 114 111 75 6
BanII G_rGCy'C
Cuts at: 0 79 238 281 320 355 578 795
Size: 79 159 43 39 35 223 217
Fragments arranged by size:
223 217 159 79 43 39 35
BbeI G_GCGC'C
Cuts at: 0 115 190 310 795
Size: 115 75 120 485
Fragments arranged by size:
485 120 115 75
BglI GCCn_nnn'nGGC
Cuts at: 0 184 315 795
Size: 184 131 480
Fragments arranged by size:
480 184 131
BglII A'GATC_T
Cuts at: 0 596 795
Size: 596 199
Bme1580I G_kGCm'C
Cuts at: 0 79 238 281 320 355 361 795
Size: 79 159 43 39 35 6 434
Fragments arranged by size:
434 159 79 43 39 35 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 222 795
Size: 222 573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 269 520 526 795
Size: 120 149 251 6 269
Fragments arranged by size:
269 251 149 120 6
BsaAI yAC'GTr
Cuts at: 0 150 795
Size: 150 645
BsaHI Gr'CG_yC
Cuts at: 0 112 187 307 391 795
Size: 112 75 120 84 404
Fragments arranged by size:
404 120 112 84 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 499 529 747 777 795
Size: 499 30 218 30 18
Fragments arranged by size:
499 218 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 750 795
Size: 750 45
BseYI C'CCAG_C
Cuts at: 0 48 258 702 733 795
Size: 48 210 444 31 62
Fragments arranged by size:
444 210 62 48 31
BsiEI CG_ry'CG
Cuts at: 0 425 795
Size: 425 370
BsmI GAATG_Cn'
Cuts at: 0 271 795
Size: 271 524
Bsp1286I G_dGCh'C
Cuts at: 0 79 238 281 320 355 361 578 795
Size: 79 159 43 39 35 6 217 217
Fragments arranged by size:
217 217 159 79 43 39 35 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 579 795
Size: 579 216
BsrFI r'CCGG_y
Cuts at: 0 156 303 611 795
Size: 156 147 308 184
Fragments arranged by size:
308 184 156 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 750 795
Size: 750 45
BstYI r'GATC_y
Cuts at: 0 596 795
Size: 596 199
BtgI C'CryG_G
Cuts at: 0 239 795
Size: 239 556
EaeI y'GGCC_r
Cuts at: 0 422 695 752 795
Size: 422 273 57 43
Fragments arranged by size:
422 273 57 43
EagI C'GGCC_G
Cuts at: 0 422 795
Size: 422 373
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 402 561 795
Size: 402 159 234
Fragments arranged by size:
402 234 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 269 520 526 795
Size: 120 149 251 6 269
Fragments arranged by size:
269 251 149 120 6
EcoO109I rG'GnC_Cy
Cuts at: 0 235 278 351 723 795
Size: 235 43 73 372 72
Fragments arranged by size:
372 235 73 72 43
HaeII r_GCGC'y
Cuts at: 0 115 190 310 795
Size: 115 75 120 485
Fragments arranged by size:
485 120 115 75
KasI G'GCGC_C
Cuts at: 0 111 186 306 795
Size: 111 75 120 489
Fragments arranged by size:
489 120 111 75
MscI TGG'CCA
Cuts at: 0 754 795
Size: 754 41
MspA1I CmG'CkG
Cuts at: 0 241 443 795
Size: 241 202 352
Fragments arranged by size:
352 241 202
NaeI GCC'GGC
Cuts at: 0 158 305 795
Size: 158 147 490
Fragments arranged by size:
490 158 147
NarI GG'CG_CC
Cuts at: 0 112 187 307 795
Size: 112 75 120 488
Fragments arranged by size:
488 120 112 75
NgoMIV G'CCGG_C
Cuts at: 0 156 303 795
Size: 156 147 492
Fragments arranged by size:
492 156 147
PasI CC'CwG_GG
Cuts at: 0 313 717 795
Size: 313 404 78
Fragments arranged by size:
404 313 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 498 795
Size: 498 297
PmlI CAC'GTG
Cuts at: 0 150 795
Size: 150 645
PspOMI G'GGCC_C
Cuts at: 0 75 234 277 316 351 795
Size: 75 159 43 39 35 444
Fragments arranged by size:
444 159 75 43 39 35
SacII CC_GC'GG
Cuts at: 0 242 795
Size: 242 553
SfoI GGC'GCC
Cuts at: 0 113 188 308 795
Size: 113 75 120 487
Fragments arranged by size:
487 120 113 75
SmaI CCC'GGG
Cuts at: 0 233 326 356 795
Size: 233 93 30 439
Fragments arranged by size:
439 233 93 30
SrfI GCCC'GGGC
Cuts at: 0 233 326 356 795
Size: 233 93 30 439
Fragments arranged by size:
439 233 93 30
StyI C'CwwG_G
Cuts at: 0 203 458 795
Size: 203 255 337
Fragments arranged by size:
337 255 203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 747 779 795
Size: 747 32 16
Fragments arranged by size:
747 32 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 795
Size: 69 726
XmaI C'CCGG_G
Cuts at: 0 231 324 354 795
Size: 231 93 30 441
Fragments arranged by size:
441 231 93 30
Enzymes that do cut:
AarI AccI ApaI AvaI BanI BanII BbeI BglI
BglII Bme1580I BmrI BpmI BsaAI BsaHI BsaXI BseRI
BseYI BsiEI BsmI Bsp1286I BspMI BsrFI BstXI BstYI
BtgI EaeI EagI EciI Eco57MI EcoO109I HaeII KasI
MscI MspA1I NaeI NarI NgoMIV PasI PflMI PmlI
PspOMI SacII SfoI SmaI SrfI StyI TstI XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaWI BsgI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI StuI SwaI TaqII TatI Tth111I XbaI
XhoI XmnI ZraI