Restriction map for
pFN21AB9695
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AvrII BalI
BamHI BbrPI BbsI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfcI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AlwI 774 GGATC GGATC(4/5)
AocI 755 CCTCAGG CC^T[ACGT]AGG
ApaI 205 GGGCCC GGGCC^C
AspHI 714 GTGCTC G[AT]GC[AT]^C
BanII 205 GGGCCC G[GA]GC[CT]^C
BbvCI 704 CCTCAGC CCTCAGC(-5/-2)
BcgI 530 GCATCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 207 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 575 AGATCT A^GATCT
BpmI 484 CTGGAG CTGGAG(16/14)
BsaI 121 GGTCTC GGTCTC(1/5)
BsgI 115 CTGCAC GTGCAG(16/14)
BsiHKAI 714 GTGCTC G[AT]GC[AT]^C
BsmFI 516 GTCCC GGGAC(10/14)
BspMI 567 GCAGGT ACCTGC(4/8)
BsrFI 655 GCCGGT [GA]^CCGG[CT]
BssHII 145 GCGCGC G^CGCGC
BstAPI 108 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 755 CCTCAGG CC^T[ACGT]AGG
Cfr10I 655 GCCGGT [GA]^CCGG[CT]
DraII 205 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 329 CGGCCG [CT]^GGCC[GA]
EagI 329 CGGCCG C^GGCCG
EarI 82 CTCTTC CTCTTC(1/4)
EclXI 329 CGGCCG C^GGCCG
Eco52I 329 CGGCCG C^GGCCG
Eco81I 755 CCTCAGG CC^T[ACGT]AGG
EcoO109I 205 GGGCCCC [GA]G^G[ACGT]CC[CT]
HgaI 182 GCGTC GACGC(5/10)
Ksp632I 82 CTCTTC CTCTTC(1/4)
MaeII 525 ACGT A^CGT
MslI 402 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 539 TCGCGA TCG^CGA
PleI 22 GACTC GAGTC(4/5)
SapI 81 GCTCTTC GCTCTTC(1/4)
ScaI 266 AGTACT AGT^ACT
SfiI 206 GGCCCCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 235 CTCAAG C^T[CT][GA]AG
TaqI 533 TCGA T^CGA
TfiI 552 GAATC G^A[AT]TC
Tsp509I 734 AATT ^AATT
TspRI 397 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 533 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 57 GGCGCC G[GA]^CG[CT]C
133 GGCGCC
AfaI 267 GTAC GT^AC
510 GTAC
AviII 449 TGCGCA TGC^GCA
691 TGCGCA
BbeI 57 GGCGCC GGCGC^C
133 GGCGCC
BfaI 28 CTAG C^TAG
478 CTAG
BsaHI 57 GGCGCC G[GA]^CG[CT]C
133 GGCGCC
BseRI 702 CTCCTC GAGGAG(10/8)
753 CTCCTC
BsiEI 329 CGGCCG CG[GA][CT]^CG
768 CGACCG
BsiYI 250 CCACCCTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
752 CCTCCTCAGGG
BslI 250 CCACCCTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
752 CCTCCTCAGGG
BsmAI 122 GTCTC GTCTC(1/5)
744 GTCTC
BsrBI 79 CCGCTC CCGCTC(-3/-3)
175 CCGCTC
BstF5I 446 GGATG GGATG(2/0)
723 GGATG
BstYI 575 AGATCT [GA]^GATC[CT]
774 GGATCT
DdeI 705 CTCAG C^T[ACGT]AG
756 CTCAG
FokI 446 GGATG GGATG(9/13)
723 GGATG
FspI 449 TGCGCA TGC^GCA
691 TGCGCA
HaeII 57 GGCGCC [GA]GCGC^[CT]
133 GGCGCC
Hin1I 57 GGCGCC G[GA]^CG[CT]C
133 GGCGCC
HinfI 22 GACTC G^A[ACGT]TC
552 GAATC
KasI 57 GGCGCC G^GCGCC
133 GGCGCC
MaeI 28 CTAG C^TAG
478 CTAG
MaeIII 184 GTCAC ^GT[ACGT]AC
784 GTCAC
MflI 575 AGATCT [GA]^GATC[CT]
774 GGATCT
NarI 57 GGCGCC GG^CGCC
133 GGCGCC
NlaIII 202 CATG CATG^
741 CATG
RsaI 267 GTAC GT^AC
510 GTAC
SfoI 57 GGCGCC GGC^GCC
133 GGCGCC
SmaI 211 CCCGGG CCC^GGG
310 CCCGGG
Tsp45I 184 GTCAC ^GT[GC]AC
784 GTCAC
XhoII 575 AGATCT [GA]^GATC[CT]
774 GGATCT
XmaCI 211 CCCGGG C^CCGGG
310 CCCGGG
XmaI 211 CCCGGG C^CCGGG
310 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AvaI BanI
BbvI BcnI BmyI BsaJI BsoBI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DpnII EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI TseI