Restriction map for
pFN21AB9694
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AclI AcsI AfaI
AflII AflIII AhdI AocI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BanII BbrPI BbsI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I EarI Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MamI MfeI MluI MslI
MunI NcoI NdeI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 187 GTCTAC GT^[AC][GT]AC
AccIII 726 TCCGGA T^CCGGA
AgeI 695 ACCGGT A^CCGGT
Alw44I 253 GTGCAC G^TGCAC
AlwNI 634 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 20 AGCGCT AGC^GCT
ApaLI 253 GTGCAC G^TGCAC
AspHI 253 GTGCAC G[AT]GC[AT]^C
AvaI 421 CCCGAG C^[CT]CG[GA]G
AvaII 84 GGACC G^G[AT]CC
BalI 47 TGGCCA TGG^CCA
BbvCI 570 CCTCAGC CCTCAGC(-5/-2)
BfaI 416 CTAG C^TAG
BmyI 253 GTGCAC G[AGT]GC[ACT]^C
BsaAI 532 TACGTG [CT]AC^GT[GA]
BsaI 222 GGTCTC GGTCTC(1/5)
BseAI 726 TCCGGA T^CCGGA
BseRI 300 CTCCTC GAGGAG(10/8)
BsiEI 158 CGGCCG CG[GA][CT]^CG
BsiHKAI 253 GTGCAC G[AT]GC[AT]^C
BsmAI 223 GTCTC GTCTC(1/5)
BsoBI 421 CCCGAG C^[CT]CG[GA]G
Bsp1286I 253 GTGCAC G[AGT]GC[ACT]^C
BspEI 726 TCCGGA T^CCGGA
BsrI 250 CCAGT ACTGG(1/-1)
BstAPI 255 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 83 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 98 CCGCGG C^C[GA][CT]GG
EagI 158 CGGCCG C^GGCCG
EclXI 158 CGGCCG C^GGCCG
Eco47III 20 AGCGCT AGC^GCT
Eco52I 158 CGGCCG C^GGCCG
EcoO109I 83 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 383 GATATC GAT^ATC
HphI 29 TCACC GGTGA(8/7)
KspI 98 CCGCGG CCGC^GG
MaeI 416 CTAG C^TAG
MaeIII 742 GTGAC ^GT[ACGT]AC
MluNI 47 TGGCCA TGG^CCA
MroI 726 TCCGGA T^CCGGA
MscI 47 TGGCCA TGG^CCA
MseI 692 TTAA T^TAA
NaeI 9 GCCGGC GCC^GGC
NgoMIV 9 GCCGGC G^CCGGC
PinAI 695 ACCGGT A^CCGGT
PpuMI 83 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 556 CAGCTG CAG^CTG
SacII 98 CCGCGG CCGC^GG
TaqI 35 TCGA T^CGA
Tru9I 692 TTAA T^TAA
Tsp45I 742 GTGAC ^GT[GC]AC
TspRI 251 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 35 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
BamHI 342 GGATCC G^GATCC
467 GGATCC
BbeI 12 GGCGCC GGCGC^C
541 GGCGCC
BcnI 198 CCCGG CC^[GC]GG
258 CCCGG
BpmI 120 CTCCAG CTGGAG(16/14)
640 CTGGAG
BsaWI 695 ACCGGT [AT]^CCGG[AT]
726 TCCGGA
BstF5I 90 CATCC GGATG(2/0)
369 CATCC
BstNI 279 CCTGG CC^[AT]GG
362 CCTGG
BstYI 342 GGATCC [GA]^GATC[CT]
467 GGATCC
Cfr13I 84 GGACC G^G[ACGT]CC
450 GGCCC
DpnII 343 GATC ^GATC
468 GATC
DraIII 256 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
529 CACTACGTG
EcoRII 279 CCTGG ^CC[AT]GG
362 CCTGG
EcoT14I 50 CCAAGG C^C[AT][AT]GG
402 CCAAGG
FokI 90 CATCC GGATG(9/13)
369 CATCC
HgaI 681 GACGC GACGC(5/10)
185 GCGTC
KasI 12 GGCGCC G^GCGCC
541 GGCGCC
MaeII 533 ACGT A^CGT
740 ACGT
MboI 343 GATC ^GATC
468 GATC
MflI 342 GGATCC [GA]^GATC[CT]
467 GGATCC
MvaI 279 CCTGG CC^[AT]GG
362 CCTGG
NarI 12 GGCGCC GG^CGCC
541 GGCGCC
NciI 198 CCCGG CC^[GC]GG
258 CCCGG
NdeII 343 GATC ^GATC
468 GATC
PleI 37 GAGTC GAGTC(4/5)
723 GAGTC
Sau3AI 343 GATC ^GATC
468 GATC
Sau96I 84 GGACC G^G[ACGT]CC
450 GGCCC
SfoI 12 GGCGCC GGC^GCC
541 GGCGCC
StyI 50 CCAAGG C^C[AT][AT]GG
402 CCAAGG
XcmI 32 CCATCGAGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
194 CCAACCCGGACTTGG
XhoII 342 GGATCC [GA]^GATC[CT]
467 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
BanI BbvI BsaHI BsaJI BsiYI
BslI BsmFI BsrFI BssKI BstUI
Cac8I CfoI Cfr10I DdeI EaeI
FauI Fnu4HI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
ItaI MboII MnlI MspA1I MspI
MvnI MwoI NlaIV ScrFI TfiI
TseI