Restriction map for
pFN21AB9694
(Linear) (Six-base) MAPSORT of: KIBB9694.seq Check: 6884 from: 1 to: 756
KIBB9694.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 188 756
Size: 188 568
AfeI AGC'GCT
Cuts at: 0 22 756
Size: 22 734
AgeI A'CCGG_T
Cuts at: 0 695 756
Size: 695 61
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 756
Size: 193 32 531
Fragments arranged by size:
531 193 32
AlwNI CAG_nnn'CTG
Cuts at: 0 639 756
Size: 639 117
ApaLI G'TGCA_C
Cuts at: 0 253 756
Size: 253 503
AvaI C'yCGr_G
Cuts at: 0 421 756
Size: 421 335
BamHI G'GATC_C
Cuts at: 0 342 467 756
Size: 342 125 289
Fragments arranged by size:
342 289 125
BanI G'GyrC_C
Cuts at: 0 12 261 541 756
Size: 12 249 280 215
Fragments arranged by size:
280 249 215 12
BbeI G_GCGC'C
Cuts at: 0 16 545 756
Size: 16 529 211
Fragments arranged by size:
529 211 16
BbvCI CC'TCA_GC
Cuts at: 0 571 756
Size: 571 185
Bme1580I G_kGCm'C
Cuts at: 0 257 756
Size: 257 499
BmgBI CAC'GTC
Cuts at: 0 741 756
Size: 741 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 240 756
Size: 240 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 661 756
Size: 105 556 95
Fragments arranged by size:
556 105 95
Bpu10I CC'TnA_GC
Cuts at: 0 571 756
Size: 571 185
BsaI GGTCTCn'nnnn_
Cuts at: 0 228 756
Size: 228 528
BsaAI yAC'GTr
Cuts at: 0 534 756
Size: 534 222
BsaHI Gr'CG_yC
Cuts at: 0 13 185 542 756
Size: 13 172 357 214
Fragments arranged by size:
357 214 172 13
BsaWI w'CCGG_w
Cuts at: 0 695 726 756
Size: 695 31 30
Fragments arranged by size:
695 31 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 756
Size: 103 30 623
Fragments arranged by size:
623 103 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 756
Size: 291 465
BseYI C'CCAG_C
Cuts at: 0 491 756
Size: 491 265
BsiEI CG_ry'CG
Cuts at: 0 161 756
Size: 161 595
BsiHKAI G_wGCw'C
Cuts at: 0 257 756
Size: 257 499
Bsp1286I G_dGCh'C
Cuts at: 0 257 756
Size: 257 499
BspEI T'CCGG_A
Cuts at: 0 726 756
Size: 726 30
BsrFI r'CCGG_y
Cuts at: 0 9 175 695 756
Size: 9 166 520 61
Fragments arranged by size:
520 166 61 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 756
Size: 261 495
BstYI r'GATC_y
Cuts at: 0 342 467 756
Size: 342 125 289
Fragments arranged by size:
342 289 125
BtgI C'CryG_G
Cuts at: 0 98 756
Size: 98 658
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 379 756
Size: 379 377
DraIII CAC_nnn'GTG
Cuts at: 0 261 534 756
Size: 261 273 222
Fragments arranged by size:
273 261 222
EaeI y'GGCC_r
Cuts at: 0 47 158 281 756
Size: 47 111 123 475
Fragments arranged by size:
475 123 111 47
EagI C'GGCC_G
Cuts at: 0 158 756
Size: 158 598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 661 756
Size: 105 556 95
Fragments arranged by size:
556 105 95
EcoO109I rG'GnC_Cy
Cuts at: 0 84 756
Size: 84 672
EcoRV GAT'ATC
Cuts at: 0 385 756
Size: 385 371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 42 756
Size: 42 714
HaeII r_GCGC'y
Cuts at: 0 16 24 545 756
Size: 16 8 521 211
Fragments arranged by size:
521 211 16 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 730 756
Size: 730 26
KasI G'GCGC_C
Cuts at: 0 12 541 756
Size: 12 529 215
Fragments arranged by size:
529 215 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 756
Size: 218 538
MscI TGG'CCA
Cuts at: 0 49 756
Size: 49 707
MspA1I CmG'CkG
Cuts at: 0 100 339 558 756
Size: 100 239 219 198
Fragments arranged by size:
239 219 198 100
NaeI GCC'GGC
Cuts at: 0 11 756
Size: 11 745
NarI GG'CG_CC
Cuts at: 0 13 542 756
Size: 13 529 214
Fragments arranged by size:
529 214 13
NgoMIV G'CCGG_C
Cuts at: 0 9 756
Size: 9 747
PpuMI rG'GwC_Cy
Cuts at: 0 84 756
Size: 84 672
PvuII CAG'CTG
Cuts at: 0 558 756
Size: 558 198
SacII CC_GC'GG
Cuts at: 0 101 756
Size: 101 655
SfoI GGC'GCC
Cuts at: 0 14 543 756
Size: 14 529 213
Fragments arranged by size:
529 213 14
StyI C'CwwG_G
Cuts at: 0 50 402 756
Size: 50 352 354
Fragments arranged by size:
354 352 50
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 201 756
Size: 39 162 555
Fragments arranged by size:
555 162 39
Enzymes that do cut:
AccI AfeI AgeI AloI AlwNI ApaLI AvaI BamHI
BanI BbeI BbvCI Bme1580I BmgBI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BsrFI BstAPI BstYI BtgI BtgZI
DraIII EaeI EagI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I KasI MmeI MscI MspA1I NaeI NarI NgoMIV
PpuMI PvuII SacII SfoI StyI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BanII BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmrI BmtI BpuEI BsaBI BsgI BsiWI
BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DrdI EarI EciI EcoICRI EcoNI EcoRI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MslI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI