(Circular) (Six-base) MAPSORT of: pFN21AB9692.seq Check: 8538 from: 1 to: 5434 pFN21AB9692 5434 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3244 278 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2777 1937 Size: 840 4594 Acc65I G'GTAC_C Cuts at: 2755 2755 Size: 5434 AclI AA'CG_TT Cuts at: 1563 3561 3934 1563 Size: 1998 373 3063 Fragments arranged by size: 3063 1998 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3497 4881 5149 1632 Size: 1865 1384 268 1917 Fragments arranged by size: 1917 1865 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5237 AflIII A'CryG_T Cuts at: 1203 4333 5310 1203 Size: 3130 977 1327 Fragments arranged by size: 3130 1327 977 AgeI A'CCGG_T Cuts at: 4265 4265 Size: 5434 AhdI GACnn_n'nnGTC Cuts at: 4163 4163 Size: 5434 AleI CACnn'nnGTG Cuts at: 1166 2117 1166 Size: 951 4483 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5434 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 2480 Size: 32 5402 AlwNI CAG_nnn'CTG Cuts at: 2098 2479 2677 3177 4749 2098 Size: 381 198 500 1572 2783 Fragments arranged by size: 2783 1572 500 381 198 ApaI G_GGCC'C Cuts at: 2689 2689 Size: 5434 ApaLI G'TGCA_C Cuts at: 3491 4647 3491 Size: 1156 4278 ApoI r'AATT_y Cuts at: 1493 2743 2969 3121 5230 1493 Size: 1250 226 152 2109 1697 Fragments arranged by size: 2109 1697 1250 226 152 AseI AT'TA_AT Cuts at: 160 1032 3988 160 Size: 872 2956 1606 Fragments arranged by size: 2956 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 AvaI C'yCGr_G Cuts at: 1870 1943 2759 1870 Size: 73 816 4545 Fragments arranged by size: 4545 816 73 BamHI G'GATC_C Cuts at: 2305 2764 3131 2305 Size: 459 367 4608 Fragments arranged by size: 4608 459 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2755 4210 618 Size: 325 217 627 968 1455 1842 Fragments arranged by size: 1842 1455 968 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2378 2390 2689 2753 1391 Size: 621 366 12 299 64 4072 Fragments arranged by size: 4072 621 366 299 64 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3625 3659 1903 Size: 34 1688 34 3678 Fragments arranged by size: 3678 1688 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3326 4536 3326 Size: 1210 4224 BclI T'GATC_A Cuts at: 1548 1548 Size: 5434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2693 4045 136 Size: 107 122 71 631 605 1021 1352 1525 Fragments arranged by size: 1525 1352 1021 631 605 122 107 71 BglII A'GATC_T Cuts at: 5429 5429 Size: 5434 BlpI GC'TnA_GC Cuts at: 2851 2851 Size: 5434 Bme1580I G_kGCm'C Cuts at: 1790 2689 3495 4651 1790 Size: 899 806 1156 2573 Fragments arranged by size: 2573 1156 899 806 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3165 4123 442 Size: 416 53 83 399 24 1748 958 1753 Fragments arranged by size: 1753 1748 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5434 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2507 2684 2699 4094 1239 Size: 1262 6 177 15 1395 2579 Fragments arranged by size: 2579 1395 1262 177 15 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3559 4424 4722 3559 Size: 865 298 4271 Fragments arranged by size: 4271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4097 882 Size: 617 2598 2219 Fragments arranged by size: 2598 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3225 493 Size: 711 2021 2702 Fragments arranged by size: 2702 2021 711 BsaBI GATnn'nnATC Cuts at: 5428 5428 Size: 5434 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3241 3623 4258 275 Size: 53 83 186 936 1708 382 635 1451 Fragments arranged by size: 1708 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2592 3867 4265 4539 4686 5097 2592 Size: 1275 398 274 147 411 2929 Fragments arranged by size: 2929 1275 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2480 2510 1178 Size: 30 165 30 1077 30 4102 Fragments arranged by size: 4102 1077 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 2672 1186 Size: 1192 294 3948 Fragments arranged by size: 3948 1192 294 BseYI C'CCAG_C Cuts at: 2129 2636 4637 5133 2129 Size: 507 2001 496 2430 Fragments arranged by size: 2430 2001 507 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 1163 Size: 573 603 4258 Fragments arranged by size: 4258 603 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2107 2884 3645 4673 1540 Size: 275 186 106 777 761 1028 2301 Fragments arranged by size: 2301 1028 777 761 275 186 106 BsiHKAI G_wGCw'C Cuts at: 2012 3495 3580 4651 2012 Size: 1483 85 1071 2795 Fragments arranged by size: 2795 1483 1071 85 BsmI GAATG_Cn' Cuts at: 2945 3038 2945 Size: 93 5341 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2378 2390 2689 2753 3495 3580 Size: 399 222 366 12 299 64 742 85 Cuts at: 3580 4651 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 299 222 85 64 12 BspEI T'CCGG_A Cuts at: 5097 5097 Size: 5434 BspHI T'CATG_A Cuts at: 3323 5053 3323 Size: 1730 3704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2789 844 Size: 1945 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2683 3321 1394 Size: 429 860 638 3507 Fragments arranged by size: 3507 860 638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3929 4103 65 Size: 1183 2681 174 1396 Fragments arranged by size: 2681 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4078 4265 1700 Size: 110 2268 187 2869 Fragments arranged by size: 2869 2268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5434 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5434 BssSI C'ACGA_G Cuts at: 3494 4506 3494 Size: 1012 4422 BstAPI GCAn_nnn'nTGC Cuts at: 794 2646 794 Size: 1852 3582 BstBI TT'CG_AA Cuts at: 4238 4238 Size: 5434 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5434 BstXI CCAn_nnnn'nTGG Cuts at: 2356 2356 Size: 5434 BstYI r'GATC_y Cuts at: 1972 2305 2764 3131 3516 3533 4985 5071 5083 Size: 333 459 367 385 17 1452 86 12 Cuts at: 5083 5429 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 459 385 367 346 333 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2115 2179 2199 2229 2239 513 Size: 552 1050 64 20 30 10 3708 Fragments arranged by size: 3708 1050 552 64 30 20 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4695 Fragments arranged by size: 4695 683 56 BtsI GCAGTG_nn' Cuts at: 2950 3744 3764 2950 Size: 794 20 4620 Fragments arranged by size: 4620 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5434 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5399 DraI TTT'AAA Cuts at: 2738 2738 Size: 5434 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4441 809 Size: 507 3125 1802 Fragments arranged by size: 3125 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 2881 3770 Size: 54 1425 50 275 18 274 777 889 Cuts at: 3770 5399 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 777 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2104 2881 1537 Size: 275 292 777 4090 Fragments arranged by size: 4090 777 292 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2411 3364 1353 Size: 1058 953 3423 Fragments arranged by size: 3423 1058 953 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2714 4021 4395 4541 387 Size: 1064 138 1125 1307 374 146 1280 Fragments arranged by size: 1307 1280 1125 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 5434 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2501 2507 2684 2699 3497 4094 4881 Size: 393 869 6 177 15 798 597 787 Cuts at: 4881 5149 1239 Size: 268 1524 Fragments arranged by size: 1524 869 798 787 597 393 268 177 15 6 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2360 Size: 5434 EcoO109I rG'GnC_Cy Cuts at: 2095 2245 2686 2095 Size: 150 441 4843 Fragments arranged by size: 4843 441 150 EcoRI G'AATT_C Cuts at: 2743 3121 2743 Size: 378 5056 FspI TGC'GCA Cuts at: 2301 3940 2301 Size: 1639 3795 HaeII r_GCGC'y Cuts at: 1678 4581 1678 Size: 2903 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4075 4107 4149 4181 1373 Size: 32 135 32 2503 32 42 32 2626 Fragments arranged by size: 2626 2503 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2023 2778 3024 669 Size: 988 281 85 755 246 3079 Fragments arranged by size: 3079 988 755 281 246 85 HindIII A'AGCT_T Cuts at: 748 2336 748 Size: 1588 3846 HpaI GTT'AAC Cuts at: 3024 3024 Size: 5434 KpnI G_GTAC'C Cuts at: 2759 2759 Size: 5434 MfeI C'AATT_G Cuts at: 3033 3033 Size: 5434 MluI A'CGCG_T Cuts at: 5310 5310 Size: 5434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4548 4732 1305 Size: 3243 184 2007 Fragments arranged by size: 3243 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3612 Fragments arranged by size: 3612 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 3392 3751 3910 518 Size: 648 951 1275 359 159 2042 Fragments arranged by size: 2042 1275 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2136 2275 2352 2850 3527 4675 4920 1606 Size: 530 139 77 498 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 530 498 245 139 77 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4882 NdeI CA'TA_TG Cuts at: 387 387 Size: 5434 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5434 NotI GC'GGCC_GC Cuts at: 2881 2881 Size: 5434 NspI r_CATG'y Cuts at: 1132 1229 2792 4337 1132 Size: 97 1563 1545 2229 Fragments arranged by size: 2229 1563 1545 97 PasI CC'CwG_GG Cuts at: 1348 2551 1348 Size: 1203 4231 PciI A'CATG_T Cuts at: 4333 4333 Size: 5434 PflMI CCAn_nnn'nTGG Cuts at: 1108 2098 2479 1108 Size: 990 381 4063 Fragments arranged by size: 4063 990 381 PfoI T'CCnGG_A Cuts at: 2166 2166 Size: 5434 PmeI GTTT'AAAC Cuts at: 2738 2738 Size: 5434 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2480 2512 3503 3535 5016 5048 2480 Size: 32 991 32 1481 32 2866 Fragments arranged by size: 2866 1481 991 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2095 2245 2095 Size: 150 5284 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5434 PsiI TTA'TAA Cuts at: 3004 3004 Size: 5434 PspOMI G'GGCC_C Cuts at: 2685 2685 Size: 5434 PstI C_TGCA'G Cuts at: 830 2786 830 Size: 1956 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5434 SacI G_AGCT'C Cuts at: 2012 2012 Size: 5434 SalI G'TCGA_C Cuts at: 1936 2776 1936 Size: 840 4594 SanDI GG'GwC_CC Cuts at: 2245 2245 Size: 5434 SapI GCTCTTCn'nnn_ Cuts at: 2411 2411 Size: 5434 SbfI CC_TGCA'GG Cuts at: 2786 2786 Size: 5434 ScaI AGT'ACT Cuts at: 3682 5394 3682 Size: 1712 3722 SexAI A'CCwGG_T Cuts at: 2313 2313 Size: 5434 SfcI C'TryA_G Cuts at: 826 1045 2461 2782 3917 4598 4789 826 Size: 219 1416 321 1135 681 191 1471 Fragments arranged by size: 1471 1416 1135 681 321 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5434 SmaI CCC'GGG Cuts at: 1872 2761 1872 Size: 889 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3538 4439 4701 820 Size: 197 926 1595 901 262 1553 Fragments arranged by size: 1595 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3225 493 Size: 2732 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5434 SphI G_CATG'C Cuts at: 1132 2792 1132 Size: 1660 3774 SspI AAT'ATT Cuts at: 5 52 3358 5 Size: 47 3306 2081 Fragments arranged by size: 3306 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5434 StyI C'CwwG_G Cuts at: 513 1065 2873 513 Size: 552 1808 3074 Fragments arranged by size: 3074 1808 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3461 3478 3631 3816 4261 325 Size: 1255 1881 17 153 185 445 1498 Fragments arranged by size: 1881 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3680 5392 96 Size: 274 80 33 51 1443 1703 1712 138 Fragments arranged by size: 1712 1703 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2391 2423 5394 5426 2391 Size: 32 2971 32 2399 Fragments arranged by size: 2971 2399 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5434 XbaI T'CTAG_A Cuts at: 2770 2770 Size: 5434 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5434 XmaI C'CCGG_G Cuts at: 1870 2759 1870 Size: 889 4545 XmnI GAAnn'nnTTC Cuts at: 2495 3563 2495 Size: 1068 4366 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3242 276 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SfiI SfoI SrfI SwaI XcmI