Restriction map for
pFN21AB9691
(Linear) (Six-base) MAPSORT of: KIBB9691.seq Check: 9876 from: 1 to: 690
KIBB9691.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 690
Size: 359 331
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 427 459 690
Size: 427 32 231
Fragments arranged by size:
427 231 32
AlwNI CAG_nnn'CTG
Cuts at: 0 382 426 690
Size: 382 44 264
Fragments arranged by size:
382 264 44
AvaI C'yCGr_G
Cuts at: 0 268 598 690
Size: 268 330 92
Fragments arranged by size:
330 268 92
BanI G'GyrC_C
Cuts at: 0 127 133 217 295 470 604 690
Size: 127 6 84 78 175 134 86
Fragments arranged by size:
175 134 127 86 84 78 6
BanII G_rGCy'C
Cuts at: 0 193 275 690
Size: 193 82 415
Fragments arranged by size:
415 193 82
BbeI G_GCGC'C
Cuts at: 0 131 137 221 299 474 608 690
Size: 131 6 84 78 175 134 82
Fragments arranged by size:
175 134 131 84 82 78 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 365 683 690
Size: 365 318 7
Fragments arranged by size:
365 318 7
BbvCI CC'TCA_GC
Cuts at: 0 338 690
Size: 338 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 267 301 468 502 690
Size: 267 34 167 34 188
Fragments arranged by size:
267 188 167 34 34
BglII A'GATC_T
Cuts at: 0 524 690
Size: 524 166
Bme1580I G_kGCm'C
Cuts at: 0 614 690
Size: 614 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 454 690
Size: 448 6 236
Fragments arranged by size:
448 236 6
Bpu10I CC'TnA_GC
Cuts at: 0 338 690
Size: 338 352
BsaBI GATnn'nnATC
Cuts at: 0 523 690
Size: 523 167
BsaHI Gr'CG_yC
Cuts at: 0 128 134 218 224 296 471 605 690
Size: 128 6 84 6 72 175 134 85
Fragments arranged by size:
175 134 128 85 84 72 6 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 427 457 690
Size: 427 30 233
Fragments arranged by size:
427 233 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 667 690
Size: 667 23
BsiEI CG_ry'CG
Cuts at: 0 149 690
Size: 149 541
Bsp1286I G_dGCh'C
Cuts at: 0 193 275 614 690
Size: 193 82 339 76
Fragments arranged by size:
339 193 82 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 245 690
Size: 245 445
BsrFI r'CCGG_y
Cuts at: 0 130 690
Size: 130 560
BssHII G'CGCG_C
Cuts at: 0 320 690
Size: 320 370
BstYI r'GATC_y
Cuts at: 0 524 690
Size: 524 166
BtgI C'CryG_G
Cuts at: 0 635 690
Size: 635 55
BtsI GCAGTG_nn'
Cuts at: 0 285 626 690
Size: 285 341 64
Fragments arranged by size:
341 285 64
EarI CTCTTCn'nnn_
Cuts at: 0 665 690
Size: 665 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 448 454 690
Size: 359 89 6 236
Fragments arranged by size:
359 236 89 6
EcoO109I rG'GnC_Cy
Cuts at: 0 379 690
Size: 379 311
HaeII r_GCGC'y
Cuts at: 0 131 137 221 299 474 516 608 690
Size: 131 6 84 78 175 42 92 82
Fragments arranged by size:
175 131 92 84 82 78 42 6
HincII GTy'rAC
Cuts at: 0 171 690
Size: 171 519
KasI G'GCGC_C
Cuts at: 0 127 133 217 295 470 604 690
Size: 127 6 84 78 175 134 86
Fragments arranged by size:
175 134 127 86 84 78 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 690
Size: 604 86
MspA1I CmG'CkG
Cuts at: 0 637 690
Size: 637 53
NaeI GCC'GGC
Cuts at: 0 132 690
Size: 132 558
NarI GG'CG_CC
Cuts at: 0 128 134 218 296 471 605 690
Size: 128 6 84 78 175 134 85
Fragments arranged by size:
175 134 128 85 84 78 6
NgoMIV G'CCGG_C
Cuts at: 0 130 690
Size: 130 560
PflMI CCAn_nnn'nTGG
Cuts at: 0 426 690
Size: 426 264
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 427 459 690
Size: 427 32 231
Fragments arranged by size:
427 231 32
PpuMI rG'GwC_Cy
Cuts at: 0 379 690
Size: 379 311
PshAI GACnn'nnGTC
Cuts at: 0 575 690
Size: 575 115
PstI C_TGCA'G
Cuts at: 0 280 690
Size: 280 410
SacII CC_GC'GG
Cuts at: 0 638 690
Size: 638 52
SfcI C'TryA_G
Cuts at: 0 276 690
Size: 276 414
SfoI GGC'GCC
Cuts at: 0 129 135 219 297 472 606 690
Size: 129 6 84 78 175 134 84
Fragments arranged by size:
175 134 129 84 84 78 6
SgrAI Cr'CCGG_yG
Cuts at: 0 130 690
Size: 130 560
SmaI CCC'GGG
Cuts at: 0 270 690
Size: 270 420
XmaI C'CCGG_G
Cuts at: 0 268 690
Size: 268 422
XmnI GAAnn'nnTTC
Cuts at: 0 442 690
Size: 442 248
Enzymes that do cut:
AcuI AloI AlwNI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglII Bme1580I BpmI Bpu10I BsaBI BsaHI
BsaXI BseRI BsiEI Bsp1286I BspMI BsrFI BssHII BstYI
BtgI BtsI EarI Eco57MI EcoO109I HaeII HincII KasI
MmeI MspA1I NaeI NarI NgoMIV PflMI PpiI PpuMI
PshAI PstI SacII SfcI SfoI SgrAI SmaI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaWI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI MscI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI ZraI