(Circular) (Six-base) MAPSORT of: pFN21AB9690.seq Check: 5834 from: 1 to: 5383 pFN21AB9690 5383 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2082 3193 278 Size: 53 83 186 936 546 1111 2468 Fragments arranged by size: 2468 1111 936 546 186 83 53 AccI GT'mk_AC Cuts at: 1937 2726 1937 Size: 789 4594 Acc65I G'GTAC_C Cuts at: 2704 2704 Size: 5383 AclI AA'CG_TT Cuts at: 1563 3510 3883 1563 Size: 1947 373 3063 Fragments arranged by size: 3063 1947 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2429 3446 4830 5098 1632 Size: 797 1017 1384 268 1917 Fragments arranged by size: 1917 1384 1017 797 268 AfeI AGC'GCT Cuts at: 2315 2423 2315 Size: 108 5275 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5186 AflIII A'CryG_T Cuts at: 1203 4282 5259 1203 Size: 3079 977 1327 Fragments arranged by size: 3079 1327 977 AgeI A'CCGG_T Cuts at: 2300 4214 2300 Size: 1914 3469 AhdI GACnn_n'nnGTC Cuts at: 4112 4112 Size: 5383 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5383 AlwNI CAG_nnn'CTG Cuts at: 3126 4698 3126 Size: 1572 3811 ApaI G_GGCC'C Cuts at: 2115 2115 Size: 5383 ApaLI G'TGCA_C Cuts at: 3440 4596 3440 Size: 1156 4227 ApoI r'AATT_y Cuts at: 1493 2692 2918 3070 5179 1493 Size: 1199 226 152 2109 1697 Fragments arranged by size: 2109 1697 1199 226 152 AseI AT'TA_AT Cuts at: 160 1032 3937 160 Size: 872 2905 1606 Fragments arranged by size: 2905 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 AvaI C'yCGr_G Cuts at: 1870 1943 2108 2219 2307 2708 1870 Size: 73 165 111 88 401 4545 Fragments arranged by size: 4545 401 165 111 88 73 BamHI G'GATC_C Cuts at: 2713 3080 2713 Size: 367 5016 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2089 2297 2704 4159 618 Size: 325 217 627 302 208 407 1455 1842 Fragments arranged by size: 1842 1455 627 407 325 302 217 208 BanII G_rGCy'C Cuts at: 1391 2115 2602 2702 1391 Size: 724 487 100 4072 Fragments arranged by size: 4072 724 487 100 BbeI G_GCGC'C Cuts at: 2093 2093 Size: 5383 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2181 928 Size: 971 282 4130 Fragments arranged by size: 4130 971 282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2298 2332 3574 3608 1903 Size: 34 361 34 1242 34 3678 Fragments arranged by size: 3678 1242 361 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3275 4485 3275 Size: 1210 4173 BclI T'GATC_A Cuts at: 1548 1548 Size: 5383 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2110 2412 2501 Size: 107 122 71 631 605 438 302 89 Cuts at: 2501 3994 136 Size: 1493 1525 Fragments arranged by size: 1525 1493 631 605 438 302 122 107 89 71 BglII A'GATC_T Cuts at: 5378 5378 Size: 5383 BlpI GC'TnA_GC Cuts at: 2354 2800 2354 Size: 446 4937 Bme1580I G_kGCm'C Cuts at: 1790 2115 3444 4600 1790 Size: 325 1329 1156 2573 Fragments arranged by size: 2573 1329 1156 325 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5383 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2050 2598 3114 Size: 416 53 83 399 24 633 548 516 Cuts at: 3114 4072 442 Size: 958 1753 Fragments arranged by size: 1753 958 633 548 516 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5383 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 4043 1239 Size: 845 1959 2579 Fragments arranged by size: 2579 1959 845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3508 4373 4671 3508 Size: 865 298 4220 Fragments arranged by size: 4220 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4046 882 Size: 617 2547 2219 Fragments arranged by size: 2547 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3174 493 Size: 711 1970 2702 Fragments arranged by size: 2702 1970 711 BsaBI GATnn'nnATC Cuts at: 5377 5377 Size: 5383 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 2090 3190 3572 Size: 53 83 186 936 546 11 1100 382 Cuts at: 3572 4207 275 Size: 635 1451 Fragments arranged by size: 1451 1100 936 635 546 382 186 83 53 11 BsaWI w'CCGG_w Cuts at: 2300 2508 3816 4214 4488 4635 5046 2300 Size: 208 1308 398 274 147 411 2637 Fragments arranged by size: 2637 1308 411 398 274 208 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5158 Fragments arranged by size: 5158 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2216 1186 Size: 1030 4353 BseYI C'CCAG_C Cuts at: 2114 4586 5082 2114 Size: 2472 496 2415 Fragments arranged by size: 2472 2415 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2302 1163 Size: 573 566 4244 Fragments arranged by size: 4244 573 566 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 2833 3594 4622 1540 Size: 275 186 33 799 761 1028 2301 Fragments arranged by size: 2301 1028 799 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 3444 3529 4600 3444 Size: 85 1071 4227 Fragments arranged by size: 4227 1071 85 BsmI GAATG_Cn' Cuts at: 2894 2987 2894 Size: 93 5290 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2602 2702 3444 3529 4600 1391 Size: 399 325 487 100 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 399 325 100 85 BspEI T'CCGG_A Cuts at: 2508 5046 2508 Size: 2538 2845 BspHI T'CATG_A Cuts at: 3272 5002 3272 Size: 1730 3653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 844 Size: 1894 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2272 2476 3270 1394 Size: 429 449 204 794 3507 Fragments arranged by size: 3507 794 449 429 204 BsrDI GCAATG_nn' Cuts at: 65 1248 3878 4052 65 Size: 1183 2630 174 1396 Fragments arranged by size: 2630 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2125 2294 2300 4027 4214 1700 Size: 110 315 169 6 1727 187 2869 Fragments arranged by size: 2869 1727 315 187 169 110 6 BsrGI T'GTAC_A Cuts at: 96 2664 96 Size: 2568 2815 BssHII G'CGCG_C Cuts at: 1925 2373 2375 1925 Size: 448 2 4933 Fragments arranged by size: 4933 448 2 BssSI C'ACGA_G Cuts at: 3443 4455 3443 Size: 1012 4371 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5383 BstBI TT'CG_AA Cuts at: 4187 4187 Size: 5383 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5383 BstXI CCAn_nnnn'nTGG Cuts at: 2611 2611 Size: 5383 BstYI r'GATC_y Cuts at: 1972 2713 3080 3465 3482 4934 5020 5032 5378 Size: 741 367 385 17 1452 86 12 346 Cuts at: 5378 1972 Size: 1977 Fragments arranged by size: 1977 1452 741 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2280 2280 Size: 5383 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2093 2325 2389 513 Size: 552 939 24 65 232 64 3507 Fragments arranged by size: 3507 939 552 232 65 64 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4644 Fragments arranged by size: 4644 683 56 BtsI GCAGTG_nn' Cuts at: 2899 3693 3713 2899 Size: 794 20 4569 Fragments arranged by size: 4569 794 20 ClaI AT'CG_AT Cuts at: 1554 2049 1554 Size: 495 4888 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5348 DraI TTT'AAA Cuts at: 2687 2687 Size: 5383 DraIII CAC_nnn'GTG Cuts at: 2395 2395 Size: 5383 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4390 809 Size: 507 3074 1802 Fragments arranged by size: 3074 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2031 2230 Size: 54 1425 50 275 18 177 24 199 Cuts at: 2230 2490 2613 2830 3719 5348 8 Size: 260 123 217 889 1629 43 Fragments arranged by size: 1629 1425 889 275 260 217 199 177 123 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 2830 1537 Size: 275 219 799 4090 Fragments arranged by size: 4090 799 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 2181 3313 1353 Size: 714 114 1132 3423 Fragments arranged by size: 3423 1132 714 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2144 3970 4344 4490 387 Size: 1064 138 555 1826 374 146 1280 Fragments arranged by size: 1826 1280 1064 555 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2429 3446 4043 4830 5098 1239 Size: 393 452 345 1017 597 787 268 1524 Fragments arranged by size: 1524 1017 787 597 452 393 345 268 EcoRI G'AATT_C Cuts at: 2692 3070 2692 Size: 378 5005 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2654 2654 Size: 5383 FspI TGC'GCA Cuts at: 3889 3889 Size: 5383 HaeII r_GCGC'y Cuts at: 1678 2093 2317 2425 2507 4530 1678 Size: 415 224 108 82 2023 2531 Fragments arranged by size: 2531 2023 415 224 108 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4024 4056 4098 4130 1373 Size: 32 135 32 2452 32 42 32 2626 Fragments arranged by size: 2626 2452 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2727 2973 669 Size: 988 281 789 246 3079 Fragments arranged by size: 3079 988 789 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5383 HpaI GTT'AAC Cuts at: 2973 2973 Size: 5383 KasI G'GCGC_C Cuts at: 2089 2089 Size: 5383 KpnI G_GTAC'C Cuts at: 2708 2708 Size: 5383 MfeI C'AATT_G Cuts at: 2982 2982 Size: 5383 MluI A'CGCG_T Cuts at: 5259 5259 Size: 5383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4497 4681 1305 Size: 3192 184 2007 Fragments arranged by size: 3192 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2615 10 Size: 54 1425 343 783 2778 Fragments arranged by size: 2778 1425 783 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3341 3700 3859 518 Size: 648 2175 359 159 2042 Fragments arranged by size: 2175 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2095 2245 2327 2799 3476 4624 4869 Size: 424 65 150 82 472 677 1148 245 Cuts at: 4869 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 472 424 245 150 82 65 NaeI GCC'GGC Cuts at: 2127 2296 2127 Size: 169 5214 NarI GG'CG_CC Cuts at: 2090 2090 Size: 5383 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3892 Fragments arranged by size: 3892 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5383 NgoMIV G'CCGG_C Cuts at: 2125 2294 2125 Size: 169 5214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5383 NotI GC'GGCC_GC Cuts at: 2830 2830 Size: 5383 NspI r_CATG'y Cuts at: 1132 1229 2741 4286 1132 Size: 97 1512 1545 2229 Fragments arranged by size: 2229 1545 1512 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5383 PciI A'CATG_T Cuts at: 4282 4282 Size: 5383 PflMI CCAn_nnn'nTGG Cuts at: 1108 2395 1108 Size: 1287 4096 PfoI T'CCnGG_A Cuts at: 2546 2546 Size: 5383 PmeI GTTT'AAAC Cuts at: 2687 2687 Size: 5383 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3452 3484 4965 4997 3452 Size: 32 1481 32 3838 Fragments arranged by size: 3838 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5383 PsiI TTA'TAA Cuts at: 2953 2953 Size: 5383 PspOMI G'GGCC_C Cuts at: 2111 2111 Size: 5383 PstI C_TGCA'G Cuts at: 830 2735 830 Size: 1905 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5383 SacII CC_GC'GG Cuts at: 2031 2096 2328 2031 Size: 65 232 5086 Fragments arranged by size: 5086 232 65 SalI G'TCGA_C Cuts at: 1936 2725 1936 Size: 789 4594 SbfI CC_TGCA'GG Cuts at: 2735 2735 Size: 5383 ScaI AGT'ACT Cuts at: 3631 5343 3631 Size: 1712 3671 SfcI C'TryA_G Cuts at: 826 1045 2650 2731 3866 4547 4738 826 Size: 219 1605 81 1135 681 191 1471 Fragments arranged by size: 1605 1471 1135 681 219 191 81 SfoI GGC'GCC Cuts at: 2091 2091 Size: 5383 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 SgrAI Cr'CCGG_yG Cuts at: 1700 2125 1700 Size: 425 4958 SmaI CCC'GGG Cuts at: 1872 2110 2221 2710 1872 Size: 238 111 489 4545 Fragments arranged by size: 4545 489 238 111 SmlI C'TyrA_G Cuts at: 820 1017 1943 3487 4388 4650 820 Size: 197 926 1544 901 262 1553 Fragments arranged by size: 1553 1544 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3174 493 Size: 2681 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5383 SphI G_CATG'C Cuts at: 1132 2741 1132 Size: 1609 3774 SspI AAT'ATT Cuts at: 5 52 3307 5 Size: 47 3255 2081 Fragments arranged by size: 3255 2081 47 StuI AGG'CCT Cuts at: 1517 2285 1517 Size: 768 4615 StyI C'CwwG_G Cuts at: 513 1065 2004 2638 2822 513 Size: 552 939 634 184 3074 Fragments arranged by size: 3074 939 634 552 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3410 3427 3580 3765 4210 325 Size: 1255 1830 17 153 185 445 1498 Fragments arranged by size: 1830 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2664 3629 5341 Size: 274 80 33 51 1443 687 965 1712 Cuts at: 5341 96 Size: 138 Fragments arranged by size: 1712 1443 965 687 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5343 5375 5343 Size: 32 5351 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5383 XbaI T'CTAG_A Cuts at: 2719 2719 Size: 5383 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2461 2461 Size: 5383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5383 XmaI C'CCGG_G Cuts at: 1870 2108 2219 2708 1870 Size: 238 111 489 4545 Fragments arranged by size: 4545 489 238 111 XmnI GAAnn'nnTTC Cuts at: 2561 3512 2561 Size: 951 4432 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2080 3191 276 Size: 53 83 186 936 546 1111 2468 Fragments arranged by size: 2468 1111 936 546 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI