Restriction map for
pFN21AB9690
(Linear) (Six-base) MAPSORT of: KIBB9690.seq Check: 809 from: 1 to: 678
KIBB9690.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AatII G_ACGT'C
Cuts at: 0 77 678
Size: 77 601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 678
Size: 424 254
AfeI AGC'GCT
Cuts at: 0 310 418 678
Size: 310 108 260
Fragments arranged by size:
310 260 108
AgeI A'CCGG_T
Cuts at: 0 295 678
Size: 295 383
ApaI G_GGCC'C
Cuts at: 0 110 678
Size: 110 568
AvaI C'yCGr_G
Cuts at: 0 103 214 302 678
Size: 103 111 88 376
Fragments arranged by size:
376 111 103 88
BanI G'GyrC_C
Cuts at: 0 84 292 678
Size: 84 208 386
Fragments arranged by size:
386 208 84
BanII G_rGCy'C
Cuts at: 0 110 597 678
Size: 110 487 81
Fragments arranged by size:
487 110 81
BbeI G_GCGC'C
Cuts at: 0 88 678
Size: 88 590
BbsI GAAGACnn'nnnn_
Cuts at: 0 176 678
Size: 176 502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 293 327 678
Size: 293 34 351
Fragments arranged by size:
351 293 34
BglI GCCn_nnn'nGGC
Cuts at: 0 105 407 496 678
Size: 105 302 89 182
Fragments arranged by size:
302 182 105 89
BlpI GC'TnA_GC
Cuts at: 0 349 678
Size: 349 329
Bme1580I G_kGCm'C
Cuts at: 0 110 678
Size: 110 568
BmrI ACTGGGnnnn_n'
Cuts at: 0 45 593 678
Size: 45 548 85
Fragments arranged by size:
548 85 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 678
Size: 79 599
BsaHI Gr'CG_yC
Cuts at: 0 74 85 678
Size: 74 11 593
Fragments arranged by size:
593 74 11
BsaWI w'CCGG_w
Cuts at: 0 295 503 678
Size: 295 208 175
Fragments arranged by size:
295 208 175
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 678
Size: 211 467
BseYI C'CCAG_C
Cuts at: 0 109 678
Size: 109 569
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 678
Size: 297 381
BsiEI CG_ry'CG
Cuts at: 0 29 678
Size: 29 649
Bsp1286I G_dGCh'C
Cuts at: 0 110 597 678
Size: 110 487 81
Fragments arranged by size:
487 110 81
BspEI T'CCGG_A
Cuts at: 0 503 678
Size: 503 175
BsrBI CCG'CTC
Cuts at: 0 267 471 678
Size: 267 204 207
Fragments arranged by size:
267 207 204
BsrFI r'CCGG_y
Cuts at: 0 120 289 295 678
Size: 120 169 6 383
Fragments arranged by size:
383 169 120 6
BsrGI T'GTAC_A
Cuts at: 0 659 678
Size: 659 19
BssHII G'CGCG_C
Cuts at: 0 368 370 678
Size: 368 2 308
Fragments arranged by size:
368 308 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 606 678
Size: 606 72
Bsu36I CC'TnA_GG
Cuts at: 0 275 678
Size: 275 403
BtgI C'CryG_G
Cuts at: 0 23 88 320 384 678
Size: 23 65 232 64 294
Fragments arranged by size:
294 232 65 64 23
ClaI AT'CG_AT
Cuts at: 0 44 678
Size: 44 634
DraIII CAC_nnn'GTG
Cuts at: 0 390 678
Size: 390 288
EaeI y'GGCC_r
Cuts at: 0 2 26 225 485 608 678
Size: 2 24 199 260 123 70
Fragments arranged by size:
260 199 123 70 24 2
EagI C'GGCC_G
Cuts at: 0 26 678
Size: 26 652
EarI CTCTTCn'nnn_
Cuts at: 0 62 176 678
Size: 62 114 502
Fragments arranged by size:
502 114 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 139 678
Size: 139 539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 424 678
Size: 79 345 254
Fragments arranged by size:
345 254 79
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 649 678
Size: 649 29
HaeII r_GCGC'y
Cuts at: 0 88 312 420 502 678
Size: 88 224 108 82 176
Fragments arranged by size:
224 176 108 88 82
KasI G'GCGC_C
Cuts at: 0 84 678
Size: 84 594
MscI TGG'CCA
Cuts at: 0 610 678
Size: 610 68
MspA1I CmG'CkG
Cuts at: 0 25 90 240 322 678
Size: 25 65 150 82 356
Fragments arranged by size:
356 150 82 65 25
NaeI GCC'GGC
Cuts at: 0 122 291 678
Size: 122 169 387
Fragments arranged by size:
387 169 122
NarI GG'CG_CC
Cuts at: 0 85 678
Size: 85 593
NgoMIV G'CCGG_C
Cuts at: 0 120 289 678
Size: 120 169 389
Fragments arranged by size:
389 169 120
PflMI CCAn_nnn'nTGG
Cuts at: 0 390 678
Size: 390 288
PfoI T'CCnGG_A
Cuts at: 0 541 678
Size: 541 137
PspOMI G'GGCC_C
Cuts at: 0 106 678
Size: 106 572
SacII CC_GC'GG
Cuts at: 0 26 91 323 678
Size: 26 65 232 355
Fragments arranged by size:
355 232 65 26
SfcI C'TryA_G
Cuts at: 0 645 678
Size: 645 33
SfoI GGC'GCC
Cuts at: 0 86 678
Size: 86 592
SgrAI Cr'CCGG_yG
Cuts at: 0 120 678
Size: 120 558
SmaI CCC'GGG
Cuts at: 0 105 216 678
Size: 105 111 462
Fragments arranged by size:
462 111 105
StuI AGG'CCT
Cuts at: 0 280 678
Size: 280 398
StyI C'CwwG_G
Cuts at: 0 633 678
Size: 633 45
TatI w'GTAC_w
Cuts at: 0 659 678
Size: 659 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 678
Size: 456 222
XmaI C'CCGG_G
Cuts at: 0 103 214 678
Size: 103 111 464
Fragments arranged by size:
464 111 103
XmnI GAAnn'nnTTC
Cuts at: 0 556 678
Size: 556 122
ZraI GAC'GTC
Cuts at: 0 75 678
Size: 75 603
Enzymes that do cut:
AatII AcuI AfeI AgeI ApaI AvaI BanI BanII
BbeI BbsI BcgI BglI BlpI Bme1580I BmrI BpmI
BsaHI BsaWI BseRI BseYI BsgI BsiEI Bsp1286I BspEI
BsrBI BsrFI BsrGI BssHII BstXI Bsu36I BtgI ClaI
DraIII EaeI EagI EarI EciI Eco57MI FalI HaeII
KasI MscI MspA1I NaeI NarI NgoMIV PflMI PfoI
PspOMI SacII SfcI SfoI SgrAI SmaI StuI StyI
TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrDI
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI BtsI
CspCI DraI DrdI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MslI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XhoI