(Circular) (Six-base) MAPSORT of: pFN21AB9688.seq Check: 5703 from: 1 to: 5371 pFN21AB9688 5371 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3181 278 Size: 53 83 186 936 1645 2468 Fragments arranged by size: 2468 1645 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2187 2714 1937 Size: 250 527 4594 Fragments arranged by size: 4594 527 250 Acc65I G'GTAC_C Cuts at: 2692 2692 Size: 5371 AclI AA'CG_TT Cuts at: 1563 3498 3871 1563 Size: 1935 373 3063 Fragments arranged by size: 3063 1935 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3434 4818 5086 1632 Size: 1802 1384 268 1917 Fragments arranged by size: 1917 1802 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2137 820 Size: 197 1120 4054 Fragments arranged by size: 4054 1120 197 AflIII A'CryG_T Cuts at: 1203 4270 5247 1203 Size: 3067 977 1327 Fragments arranged by size: 3067 1327 977 AgeI A'CCGG_T Cuts at: 4202 4202 Size: 5371 AhdI GACnn_n'nnGTC Cuts at: 4100 4100 Size: 5371 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5371 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5371 AlwNI CAG_nnn'CTG Cuts at: 2118 2506 3114 4686 2118 Size: 388 608 1572 2803 Fragments arranged by size: 2803 1572 608 388 ApaLI G'TGCA_C Cuts at: 3428 4584 3428 Size: 1156 4215 ApoI r'AATT_y Cuts at: 1493 2522 2680 2906 3058 5167 1493 Size: 1029 158 226 152 2109 1697 Fragments arranged by size: 2109 1697 1029 226 158 152 AseI AT'TA_AT Cuts at: 160 1032 3925 160 Size: 872 2893 1606 Fragments arranged by size: 2893 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5371 AvaI C'yCGr_G Cuts at: 1870 1943 2205 2291 2513 2696 1870 Size: 73 262 86 222 183 4545 Fragments arranged by size: 4545 262 222 183 86 73 BamHI G'GATC_C Cuts at: 2701 3068 2701 Size: 367 5004 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2692 4147 618 Size: 325 217 627 905 1455 1842 Fragments arranged by size: 1842 1455 905 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2045 2212 2690 1391 Size: 621 33 167 478 4072 Fragments arranged by size: 4072 621 478 167 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4400 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2548 2582 3562 3596 1903 Size: 34 611 34 980 34 3678 Fragments arranged by size: 3678 980 611 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3263 4473 3263 Size: 1210 4161 BclI T'GATC_A Cuts at: 1548 1548 Size: 5371 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3982 136 Size: 107 122 71 631 605 2310 1525 Fragments arranged by size: 2310 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2604 5366 2604 Size: 2762 2609 BlpI GC'TnA_GC Cuts at: 2788 2788 Size: 5371 Bme1580I G_kGCm'C Cuts at: 1790 3432 4588 1790 Size: 1642 1156 2573 Fragments arranged by size: 2573 1642 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5371 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3102 4060 442 Size: 416 53 83 399 24 1685 958 1753 Fragments arranged by size: 1753 1685 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5371 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 4031 1239 Size: 1109 1683 2579 Fragments arranged by size: 2579 1683 1109 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3496 4361 4659 3496 Size: 865 298 4208 Fragments arranged by size: 4208 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4034 882 Size: 617 2535 2219 Fragments arranged by size: 2535 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2017 3162 493 Size: 711 813 1145 2702 Fragments arranged by size: 2702 1145 813 711 BsaBI GATnn'nnATC Cuts at: 2603 5365 2603 Size: 2762 2609 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3178 3560 4195 275 Size: 53 83 186 936 1645 382 635 1451 Fragments arranged by size: 1645 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3804 4202 4476 4623 5034 3804 Size: 398 274 147 411 4141 Fragments arranged by size: 4141 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5146 Fragments arranged by size: 5146 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2271 2345 2378 1186 Size: 1085 74 33 4179 Fragments arranged by size: 4179 1085 74 33 BseYI C'CCAG_C Cuts at: 4574 5070 4574 Size: 496 4875 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 2429 1163 Size: 573 387 306 4105 Fragments arranged by size: 4105 573 387 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2221 2244 2821 3582 4610 1540 Size: 275 186 220 23 577 761 1028 2301 Fragments arranged by size: 2301 1028 761 577 275 220 186 23 BsiHKAI G_wGCw'C Cuts at: 2012 3432 3517 4588 2012 Size: 1420 85 1071 2795 Fragments arranged by size: 2795 1420 1071 85 BsmI GAATG_Cn' Cuts at: 2882 2975 2882 Size: 93 5278 BsmBI CGTCTCn'nnnn_ Cuts at: 2262 2453 2262 Size: 191 5180 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2045 2212 2690 3432 3517 4588 Size: 399 222 33 167 478 742 85 1071 Cuts at: 4588 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 478 399 222 167 85 33 BspEI T'CCGG_A Cuts at: 5034 5034 Size: 5371 BspHI T'CATG_A Cuts at: 3260 4990 3260 Size: 1730 3641 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2726 844 Size: 1882 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2246 3258 1394 Size: 429 423 1012 3507 Fragments arranged by size: 3507 1012 429 423 BsrDI GCAATG_nn' Cuts at: 65 1248 3866 4040 65 Size: 1183 2618 174 1396 Fragments arranged by size: 2618 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2407 4015 4202 1700 Size: 110 597 1608 187 2869 Fragments arranged by size: 2869 1608 597 187 110 BsrGI T'GTAC_A Cuts at: 96 2298 96 Size: 2202 3169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5371 BssSI C'ACGA_G Cuts at: 2456 3431 4443 2456 Size: 975 1012 3384 Fragments arranged by size: 3384 1012 975 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5371 BstBI TT'CG_AA Cuts at: 4175 4175 Size: 5371 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5371 BstYI r'GATC_y Cuts at: 1972 2604 2701 3068 3453 3470 4922 5008 5020 Size: 632 97 367 385 17 1452 86 12 Cuts at: 5020 5366 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 632 385 367 346 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2179 2238 2429 513 Size: 552 1114 59 191 3455 Fragments arranged by size: 3455 1114 552 191 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4632 Fragments arranged by size: 4632 683 56 BtsI GCAGTG_nn' Cuts at: 2353 2887 3681 3701 2353 Size: 534 794 20 4023 Fragments arranged by size: 4023 794 534 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5371 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5336 DraI TTT'AAA Cuts at: 2675 2675 Size: 5371 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4378 809 Size: 507 3062 1802 Fragments arranged by size: 3062 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 2818 3707 Size: 54 1425 50 275 18 411 577 889 Cuts at: 3707 5336 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 577 411 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2241 2818 1537 Size: 275 429 577 4090 Fragments arranged by size: 4090 577 429 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3301 1353 Size: 1948 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2142 3958 4332 4478 387 Size: 1064 138 553 1816 374 146 1280 Fragments arranged by size: 1816 1280 1064 553 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 5371 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2348 3434 4031 4818 5086 1239 Size: 393 716 1086 597 787 268 1524 Fragments arranged by size: 1524 1086 787 716 597 393 268 EcoRI G'AATT_C Cuts at: 2522 2680 3058 2522 Size: 158 378 4835 Fragments arranged by size: 4835 378 158 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2163 2684 2163 Size: 521 4850 FspI TGC'GCA Cuts at: 3877 3877 Size: 5371 HaeII r_GCGC'y Cuts at: 1678 2238 2327 4518 1678 Size: 560 89 2191 2531 Fragments arranged by size: 2531 2191 560 89 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2287 2319 4012 4044 4086 Size: 32 135 32 715 32 1693 32 42 Cuts at: 4086 4118 1373 Size: 32 2626 Fragments arranged by size: 2626 1693 715 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2715 2961 669 Size: 988 281 777 246 3079 Fragments arranged by size: 3079 988 777 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5371 HpaI GTT'AAC Cuts at: 2961 2961 Size: 5371 KpnI G_GTAC'C Cuts at: 2696 2696 Size: 5371 MfeI C'AATT_G Cuts at: 2970 2970 Size: 5371 MluI A'CGCG_T Cuts at: 5247 5247 Size: 5371 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 4485 4669 1305 Size: 888 2292 184 2007 Fragments arranged by size: 2292 2007 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3549 Fragments arranged by size: 3549 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3329 3688 3847 518 Size: 648 2163 359 159 2042 Fragments arranged by size: 2163 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2240 2787 3464 4612 4857 1606 Size: 634 547 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 634 547 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4819 NdeI CA'TA_TG Cuts at: 387 387 Size: 5371 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5371 NotI GC'GGCC_GC Cuts at: 2241 2818 2241 Size: 577 4794 NspI r_CATG'y Cuts at: 1132 1229 2068 2729 4274 1132 Size: 97 839 661 1545 2229 Fragments arranged by size: 2229 1545 839 661 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5371 PciI A'CATG_T Cuts at: 4270 4270 Size: 5371 PflMI CCAn_nnn'nTGG Cuts at: 1108 2506 1108 Size: 1398 3973 PfoI T'CCnGG_A Cuts at: 2248 2248 Size: 5371 PmeI GTTT'AAAC Cuts at: 2675 2675 Size: 5371 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3440 3472 4953 4985 3440 Size: 32 1481 32 3826 Fragments arranged by size: 3826 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5371 PsiI TTA'TAA Cuts at: 2941 2941 Size: 5371 PstI C_TGCA'G Cuts at: 830 2234 2723 830 Size: 1404 489 3478 Fragments arranged by size: 3478 1404 489 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5371 SacI G_AGCT'C Cuts at: 2012 2012 Size: 5371 SacII CC_GC'GG Cuts at: 2241 2241 Size: 5371 SalI G'TCGA_C Cuts at: 1936 2713 1936 Size: 777 4594 SbfI CC_TGCA'GG Cuts at: 2723 2723 Size: 5371 ScaI AGT'ACT Cuts at: 3619 5331 3619 Size: 1712 3659 SfcI C'TryA_G Cuts at: 826 1045 2230 2719 3854 4535 4726 826 Size: 219 1185 489 1135 681 191 1471 Fragments arranged by size: 1471 1185 1135 681 489 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5371 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5371 SmaI CCC'GGG Cuts at: 1872 2207 2293 2698 1872 Size: 335 86 405 4545 Fragments arranged by size: 4545 405 335 86 SmlI C'TyrA_G Cuts at: 820 1017 1943 2137 2513 3475 4376 4638 820 Size: 197 926 194 376 962 901 262 1553 Fragments arranged by size: 1553 962 926 901 376 262 197 194 SnaBI TAC'GTA Cuts at: 493 2017 3162 493 Size: 1524 1145 2702 Fragments arranged by size: 2702 1524 1145 SpeI A'CTAG_T Cuts at: 152 152 Size: 5371 SphI G_CATG'C Cuts at: 1132 2729 1132 Size: 1597 3774 SspI AAT'ATT Cuts at: 5 52 3295 5 Size: 47 3243 2081 Fragments arranged by size: 3243 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5371 StyI C'CwwG_G Cuts at: 513 1065 2810 513 Size: 552 1745 3074 Fragments arranged by size: 3074 1745 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3398 3415 3568 3753 4198 325 Size: 1255 1818 17 153 185 445 1498 Fragments arranged by size: 1818 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 3617 5329 Size: 274 80 33 51 1443 321 1319 1712 Cuts at: 5329 96 Size: 138 Fragments arranged by size: 1712 1443 1319 321 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5331 5363 5331 Size: 32 5339 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5371 XbaI T'CTAG_A Cuts at: 2707 2707 Size: 5371 XhoI C'TCGA_G Cuts at: 1943 2513 1943 Size: 570 4801 XmaI C'CCGG_G Cuts at: 1870 2205 2291 2696 1870 Size: 335 86 405 4545 Fragments arranged by size: 4545 405 335 86 XmnI GAAnn'nnTTC Cuts at: 2522 3500 2522 Size: 978 4393 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3179 276 Size: 53 83 186 936 1645 2468 Fragments arranged by size: 2468 1645 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI