Restriction map for
pFN21AB9682
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstNI BstPI BstXI
BstZ17I Bsu36I ClaI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MboII
MfeI MluI MroI MseI MslI
MunI MvaI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI SacI SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 142 GGCGCC G[GA]^CG[CT]C
ApaI 39 GGGCCC GGGCC^C
AscI 150 GGCGCGCC GG^CGCGCC
AvaI 352 CCCGGG C^[CT]CG[GA]G
BalI 119 TGGCCA TGG^CCA
BbeI 142 GGCGCC GGCGC^C
BglI 412 GCCCTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 384 GCTCAGC GC^T[ACGT]AGC
BpmI 283 CTCCAG CTGGAG(16/14)
Bpu1102I 384 GCTCAGC GC^T[ACGT]AGC
BsaBI 431 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 142 GGCGCC G[GA]^CG[CT]C
BsiEI 419 CGGCCG CG[GA][CT]^CG
BsoBI 352 CCCGGG C^[CT]CG[GA]G
BspMI 180 GCAGGT ACCTGC(4/8)
BsrI 11 CCAGT ACTGG(1/-1)
CelII 384 GCTCAGC GC^T[ACGT]AGC
CpoI 371 CGGTCCG CG^G[AT]CCG
DsaI 35 CCGCGG C^C[GA][CT]GG
EagI 419 CGGCCG C^GGCCG
EclXI 419 CGGCCG C^GGCCG
Eco52I 419 CGGCCG C^GGCCG
Hin1I 142 GGCGCC G[GA]^CG[CT]C
KasI 142 GGCGCC G^GCGCC
KspI 35 CCGCGG CCGC^GG
MaeIII 215 GTTAC ^GT[ACGT]AC
MamI 431 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 119 TGGCCA TGG^CCA
MscI 119 TGGCCA TGG^CCA
NaeI 61 GCCGGC GCC^GGC
NarI 142 GGCGCC GG^CGCC
NgoMIV 61 GCCGGC G^CCGGC
NlaIII 204 CATG CATG^
PvuII 45 CAGCTG CAG^CTG
RsrII 371 CGGTCCG CG^G[AT]CCG
SacII 35 CCGCGG CCGC^GG
SfoI 142 GGCGCC GGC^GCC
SmaI 352 CCCGGG CCC^GGG
SmlI 226 CTCAAG C^T[CT][GA]AG
TaqI 296 TCGA T^CGA
TspRI 12 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 296 TCGA T^CGA
XmaCI 352 CCCGGG C^CCGGG
XmaI 352 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 431 GATCC GGATC(4/5)
437 GATCC
BanI 142 GGCGCC G^G[CT][GA]CC
237 GGTGCC
BanII 39 GGGCCC G[GA]GC[CT]^C
377 GGGCTC
BsiYI 312 CCTTCAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
338 CCGAAGTTGGG
BslI 312 CCTTCAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
338 CCGAAGTTGGG
BsmFI 308 GGGAC GGGAC(10/14)
346 GGGAC
BsrBI 382 CCGCTC CCGCTC(-3/-3)
75 GAGCGG
BsrFI 61 GCCGGC [GA]^CCGG[CT]
369 GCCGGT
BssHII 50 GCGCGC G^CGCGC
151 GCGCGC
BstYI 430 AGATCC [GA]^GATC[CT]
436 AGATCC
Cfr10I 61 GCCGGC [GA]^CCGG[CT]
369 GCCGGT
DdeI 107 CTGAG C^T[ACGT]AG
385 CTCAG
EaeI 119 TGGCCA [CT]^GGCC[GA]
419 CGGCCG
FauI 37 GCGGG CCCGC(4/6)
82 GCGGG
HaeII 142 GGCGCC [GA]GCGC^[CT]
409 AGCGCC
HinfI 279 GATTC G^A[ACGT]TC
334 GAATC
HphI 264 GGTGA GGTGA(8/7)
426 GGTGA
MaeII 188 ACGT A^CGT
290 ACGT
MflI 430 AGATCC [GA]^GATC[CT]
436 AGATCC
MspA1I 35 CCGCGG C[AC]G^C[GT]G
45 CAGCTG
PpuMI 308 GGGACCT [GA]G^G[AT]CC[CT]
346 GGGACCC
TfiI 279 GATTC G^A[AT]TC
334 GAATC
XhoII 430 AGATCC [GA]^GATC[CT]
436 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BmyI BsaJI Bsp1286I BssKI
BstUI Cac8I CfoI Cfr13I DpnII
DraII EcoO109I Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MnlI MspI MvnI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
TseI