Restriction map for
pFN21AB9675
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# NO CUT ENZYMES ( 204 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AluI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspA1I MunI MvnI
NarI NciI NcoI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I TseI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Asp700I 209 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 79 GGTCC G^G[AT]CC
BanII 153 GGGCTC G[GA]GC[CT]^C
BmyI 153 GGGCTC G[AGT]GC[ACT]^C
BpmI 175 CTGGAG CTGGAG(16/14)
BsaAI 64 TACGTG [CT]AC^GT[GA]
BseRI 218 CTCCTC GAGGAG(10/8)
BsiYI 174 CCTGGAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 174 CCTGGAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 80 GTCCC GGGAC(10/14)
Bsp1286I 153 GGGCTC G[AGT]GC[ACT]^C
BsrFI 56 GCCGGC [GA]^CCGG[CT]
BssKI 174 CCTGG ^CC[ACGT]GG
BstAPI 90 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 174 CCTGG CC^[AT]GG
Cac8I 56 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 56 GCCGGC [GA]^CCGG[CT]
Cfr13I 79 GGTCC G^G[ACGT]CC
DdeI 72 CTCAG C^T[ACGT]AG
EaeI 128 CGGCCA [CT]^GGCC[GA]
EcoNI 174 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 174 CCTGG ^CC[AT]GG
HaeIII 129 GGCC GG^CC
HapII 57 CCGG C^CGG
HpaII 57 CCGG C^CGG
HphI 168 GGTGA GGTGA(8/7)
MaeIII 105 GTCAC ^GT[ACGT]AC
MspI 57 CCGG C^CGG
MvaI 174 CCTGG CC^[AT]GG
NaeI 56 GCCGGC GCC^GGC
NgoMIV 56 GCCGGC G^CCGGC
NlaIII 203 CATG CATG^
NlaIV 79 GGTCCC GG[ACGT]^[ACGT]CC
PleI 70 GACTC GAGTC(4/5)
Sau96I 79 GGTCC G^G[ACGT]CC
ScrFI 174 CCTGG CC^[ACGT]GG
SmlI 226 CTCAAG C^T[CT][GA]AG
TfiI 24 GATTC G^A[AT]TC
Tsp45I 105 GTCAC ^GT[GC]AC
Tsp509I 210 AATT ^AATT
XmnI 209 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AciI 99 GCGG CCGC(-3/-1)
127 GCGG
AfaI 14 GTAC GT^AC
63 GTAC
EarI 221 CTCTTC CTCTTC(1/4)
118 GAAGAG
Fnu4HI 99 GCGGC GC^[ACGT]GC
127 GCGGC
HinfI 24 GATTC G^A[ACGT]TC
70 GACTC
ItaI 99 GCGGC GC^[ACGT]GC
127 GCGGC
Ksp632I 221 CTCTTC CTCTTC(1/4)
118 GAAGAG
MaeII 65 ACGT A^CGT
192 ACGT
MboII 118 GAAGA GAAGA(8/7)
222 TCTTC
MnlI 48 CCTC CCTC(7/6)
220 CCTC
MwoI 12 GCGTACCGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
90 GCAAGGACTGC
RsaI 14 GTAC GT^AC
63 GTAC
TspRI 35 CAGTG CA[GC]TG[ACGT][ACGT]^
233 CAGTG
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# 3 OR MORE CUT ENZYMES ( 0 enzymes) #
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