(Circular) (Six-base) MAPSORT of: pFN21AB9672.seq Check: 5574 from: 1 to: 5977 pFN21AB9672 5977 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2567 2621 2628 3271 3320 1937 Size: 630 54 7 643 49 4594 Fragments arranged by size: 4594 643 630 54 49 7 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2318 2820 2865 3095 4040 5424 5692 1632 Size: 686 502 45 230 945 1384 268 1917 Fragments arranged by size: 1917 1384 945 686 502 268 230 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 2209 4876 5853 1203 Size: 1006 2667 977 1327 Fragments arranged by size: 2667 1327 1006 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 3162 1166 Size: 1996 3981 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2576 3204 1184 Size: 1392 628 3957 Fragments arranged by size: 3957 1392 628 AlwNI CAG_nnn'CTG Cuts at: 2881 3720 5292 2881 Size: 839 1572 3566 Fragments arranged by size: 3566 1572 839 ApaLI G'TGCA_C Cuts at: 2359 4034 5190 2359 Size: 1675 1156 3146 Fragments arranged by size: 3146 1675 1156 ApoI r'AATT_y Cuts at: 1493 2092 2470 2499 2652 3286 3512 3664 5773 Size: 599 378 29 153 634 226 152 2109 Cuts at: 5773 1493 Size: 1697 Fragments arranged by size: 2109 1697 634 599 378 226 153 152 29 AseI AT'TA_AT Cuts at: 160 1032 2121 4531 160 Size: 872 1089 2410 1606 Fragments arranged by size: 2410 1606 1089 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2925 3302 1870 Size: 73 982 377 4545 Fragments arranged by size: 4545 982 377 73 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2904 3298 4753 618 Size: 325 217 627 1117 394 1455 1842 Fragments arranged by size: 1842 1455 1117 627 394 325 217 BanII G_rGCy'C Cuts at: 1391 3296 1391 Size: 1905 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2430 3092 928 Size: 971 531 662 3813 Fragments arranged by size: 3813 971 662 531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2669 3869 5079 2669 Size: 1200 1210 3567 Fragments arranged by size: 3567 1210 1200 BclI T'GATC_A Cuts at: 1548 2769 1548 Size: 1221 4756 BfrBI ATG'CAT Cuts at: 2971 2971 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4588 136 Size: 107 122 71 631 605 2916 1525 Fragments arranged by size: 2916 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 2145 2363 2915 4038 5194 1790 Size: 355 218 552 1123 1156 2573 Fragments arranged by size: 2573 1156 1123 552 355 218 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3708 4666 442 Size: 416 53 83 399 24 2291 958 1753 Fragments arranged by size: 2291 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2295 2903 4637 1239 Size: 1056 608 1734 2579 Fragments arranged by size: 2579 1734 1056 608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4102 4967 5265 4102 Size: 865 298 4814 Fragments arranged by size: 4814 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3768 493 Size: 711 2564 2702 Fragments arranged by size: 2702 2564 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3784 4166 4801 275 Size: 53 83 186 936 2251 382 635 1451 Fragments arranged by size: 2251 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2043 2073 1178 Size: 30 165 30 640 30 5082 Fragments arranged by size: 5082 640 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5977 BseYI C'CCAG_C Cuts at: 5180 5676 5180 Size: 496 5481 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2256 1163 Size: 573 520 4884 Fragments arranged by size: 4884 573 520 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2281 2363 4038 4123 5194 2281 Size: 82 1675 85 1071 3064 Fragments arranged by size: 3064 1675 1071 85 82 BsmI GAATG_Cn' Cuts at: 2761 3488 3581 2761 Size: 727 93 5157 Fragments arranged by size: 5157 727 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2281 2363 2915 3296 4038 4123 Size: 399 355 136 82 552 381 742 85 Cuts at: 4123 5194 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 552 399 381 355 136 85 82 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3332 844 Size: 2488 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3864 1394 Size: 429 2041 3507 Fragments arranged by size: 3507 2041 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2560 4472 4646 65 Size: 1183 1312 1912 174 1396 Fragments arranged by size: 1912 1396 1312 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5977 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstYI r'GATC_y Cuts at: 1972 3307 3674 4059 4076 5528 5614 5626 5972 Size: 1335 367 385 17 1452 86 12 346 Cuts at: 5972 1972 Size: 1977 Fragments arranged by size: 1977 1452 1335 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3217 513 Size: 552 939 1213 3273 Fragments arranged by size: 3273 1213 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 3235 3493 4287 4307 3235 Size: 258 794 20 4905 Fragments arranged by size: 4905 794 258 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 2641 3281 2641 Size: 640 5337 DraIII CAC_nnn'GTG Cuts at: 2149 2350 2149 Size: 201 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3143 3424 4313 Size: 54 1425 50 275 18 1313 281 889 Cuts at: 4313 5942 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1313 889 281 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2538 2731 3907 1353 Size: 1185 193 1176 3423 Fragments arranged by size: 3423 1185 1176 193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2295 2318 2820 2865 2903 3095 4040 Size: 393 663 23 502 45 38 192 945 Cuts at: 4040 4637 5424 5692 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 945 787 663 597 502 393 268 192 45 38 23 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2574 2574 Size: 5977 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 5124 1678 Size: 3446 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2043 2075 2863 2895 4618 Size: 32 135 32 471 32 788 32 1723 Cuts at: 4618 4650 4692 4724 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1723 788 471 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 2367 748 Size: 1619 4358 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5091 5275 1305 Size: 3786 184 2007 Fragments arranged by size: 3786 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4155 Fragments arranged by size: 4155 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 3151 3162 3935 4294 4453 518 Size: 648 1008 977 11 773 359 159 2042 Fragments arranged by size: 2042 1008 977 773 648 359 159 11 MspA1I CmG'CkG Cuts at: 1606 2687 3393 4070 5218 5463 1606 Size: 1081 706 677 1148 245 2120 Fragments arranged by size: 2120 1148 1081 706 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3217 513 Size: 552 939 1213 3273 Fragments arranged by size: 3273 1213 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NsiI A_TGCA'T Cuts at: 2973 2973 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2213 2743 3335 4880 1132 Size: 97 984 530 592 1545 2229 Fragments arranged by size: 2229 1545 984 592 530 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 2209 4876 2209 Size: 2667 3310 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2430 1613 Size: 817 5160 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspXI vC'TCGA_Gb Cuts at: 2925 2925 Size: 5977 PstI C_TGCA'G Cuts at: 830 3329 830 Size: 2499 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2687 2687 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2568 2622 2778 3325 4460 5141 5332 Size: 219 1523 54 156 547 1135 681 191 Cuts at: 5332 826 Size: 1471 Fragments arranged by size: 1523 1471 1135 681 547 219 191 156 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2925 4081 4982 5244 820 Size: 197 926 982 1156 901 262 1553 Fragments arranged by size: 1553 1156 982 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 3274 152 Size: 3122 2855 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 3250 1517 Size: 1733 4244 StyI C'CwwG_G Cuts at: 513 1065 2004 3217 3416 513 Size: 552 939 1213 199 3074 Fragments arranged by size: 3074 1213 939 552 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4004 4021 4174 4359 4804 325 Size: 1255 2424 17 153 185 445 1498 Fragments arranged by size: 2424 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2681 3071 4223 Size: 274 80 33 51 1443 704 390 1152 Cuts at: 4223 5935 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1152 704 390 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5937 5969 5937 Size: 32 5945 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 2925 1943 Size: 982 4995 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 4106 4106 Size: 5977 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI