Restriction map for
pFN21AB9668
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI AocI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BalI BamHI BciVI
BclI BfaI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI Ksp632I MaeI MaeIII MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI ScaI
SexAI SfiI SfuI SmaI SnaBI
SpeI SphI SspBI SspI StyI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 1144 GGCGCC G[GA]^CG[CT]C
AfaI 399 GTAC GT^AC
AflII 1371 CTTAAG C^TTAAG
AflIII 401 ACACGT A^C[GA][CT]GT
AlwI 798 GATCC GGATC(4/5)
Aor51HI 1106 AGCGCT AGC^GCT
BanI 1144 GGCGCC G^G[CT][GA]CC
BbeI 1144 GGCGCC GGCGC^C
BbrPI 402 CACGTG CAC^GTG
BbsI 66 GAAGAC GAAGAC(2/6)
BbvCI 444 CCTCAGC CCTCAGC(-5/-2)
BfrI 1371 CTTAAG C^TTAAG
BpuAI 66 GAAGAC GAAGAC(2/6)
BsaAI 402 CACGTG [CT]AC^GT[GA]
BsaHI 1144 GGCGCC G[GA]^CG[CT]C
BseRI 304 GAGGAG GAGGAG(10/8)
BsmAI 544 GAGAC GTCTC(1/5)
BsmI 1252 GAATGC GAATGC(1/-1)
BspMI 27 GCAGGT ACCTGC(4/8)
BsrBI 979 GAGCGG CCGCTC(-3/-3)
BsrDI 59 GCAATG GCAATG(2/0)
BssSI 75 CACGAG CACGAG(-5/-1)
BstYI 797 AGATCC [GA]^GATC[CT]
DpnII 798 GATC ^GATC
Eco47III 1106 AGCGCT AGC^GCT
EcoNI 84 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 626 GACGC GACGC(5/10)
Hin1I 1144 GGCGCC G[GA]^CG[CT]C
HinfI 4 GAGTC G^A[ACGT]TC
HphI 976 GGTGA GGTGA(8/7)
KasI 1144 GGCGCC G^GCGCC
MaeII 403 ACGT A^CGT
MboI 798 GATC ^GATC
MflI 797 AGATCC [GA]^GATC[CT]
MseI 1372 TTAA T^TAA
NarI 1144 GGCGCC GG^CGCC
NdeII 798 GATC ^GATC
NotI 729 GCGGCCGC GC^GGCCGC
PleI 4 GAGTC GAGTC(4/5)
PmaCI 402 CACGTG CAC^GTG
PmlI 402 CACGTG CAC^GTG
PpuMI 50 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 681 CAGCTG CAG^CTG
RsaI 399 GTAC GT^AC
Sau3AI 798 GATC ^GATC
SbfI 24 CCTGCAGG CCTGCA^GG
SfoI 1144 GGCGCC GGC^GCC
SmlI 1371 CTTAAG C^T[CT][GA]AG
Sse8387I 24 CCTGCAGG CCTGCA^GG
TaqI 989 TCGA T^CGA
Tru9I 1372 TTAA T^TAA
Tsp509I 694 AATT ^AATT
TthHB8I 989 TCGA T^CGA
XhoII 797 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 946 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1297 CAGCGCCTG
ApaI 222 GGGCCC GGGCC^C
253 GGGCCC
AvaI 409 CCCGAG C^[CT]CG[GA]G
1140 CTCGGG
AvaII 20 GGACC G^G[AT]CC
51 GGTCC
AviII 1246 TGCGCA TGC^GCA
1355 TGCGCA
BsgI 1303 CTGCAC GTGCAG(16/14)
616 GTGCAG
BsiEI 597 CGGCCG CG[GA][CT]^CG
730 CGGCCG
BsoBI 409 CCCGAG C^[CT]CG[GA]G
1140 CTCGGG
BssHII 515 GCGCGC G^CGCGC
725 GCGCGC
BstAPI 491 GCAGGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
945 GCAGCTCCTGC
BstF5I 462 CATCC GGATG(2/0)
531 CATCC
BstXI 10 CCAAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
692 CCAATTTCCTGG
EagI 597 CGGCCG C^GGCCG
730 CGGCCG
EclXI 597 CGGCCG C^GGCCG
730 CGGCCG
Eco52I 597 CGGCCG C^GGCCG
730 CGGCCG
FokI 462 CATCC GGATG(9/13)
531 CATCC
FspI 1246 TGCGCA TGC^GCA
1355 TGCGCA
KspI 1185 CCGCGG CCGC^GG
1269 CCGCGG
MboII 66 GAAGA GAAGA(8/7)
795 GAAGA
MslI 609 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
970 CACACTGGTG
NlaIII 1020 CATG CATG^
1072 CATG
SacII 1185 CCGCGG CCGC^GG
1269 CCGCGG
SfaNI 369 GCATC GCATC(5/9)
461 GCATC
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI Alw44I ApaLI
Asp700I AspHI BanII BbvI BcgI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrFI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV PstI SacI Sau96I ScrFI
SfcI SgrAI StuI TseI TspRI
XmnI