(Circular) (Six-base) MAPSORT of: pFN21AB9668.seq Check: 1307 from: 1 to: 6199 pFN21AB9668 6199 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2023 2023 Size: 6199 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4009 278 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3542 1937 Size: 1605 4594 Acc65I G'GTAC_C Cuts at: 3520 3520 Size: 6199 AclI AA'CG_TT Cuts at: 1563 4326 4699 1563 Size: 2763 373 3063 Fragments arranged by size: 3063 2763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 2668 2752 2819 3088 4262 5646 5914 Size: 953 83 84 67 269 1174 1384 268 Cuts at: 5914 1632 Size: 1917 Fragments arranged by size: 1917 1384 1174 953 269 268 84 83 67 AfeI AGC'GCT Cuts at: 3113 3113 Size: 6199 AflII C'TTAA_G Cuts at: 820 1017 3376 820 Size: 197 2359 3643 Fragments arranged by size: 3643 2359 197 AflIII A'CryG_T Cuts at: 1203 2406 5098 6075 1203 Size: 1203 2692 977 1327 Fragments arranged by size: 2692 1327 1203 977 AgeI A'CCGG_T Cuts at: 5030 5030 Size: 6199 AhdI GACnn_n'nnGTC Cuts at: 4928 4928 Size: 6199 AleI CACnn'nnGTG Cuts at: 1166 2618 2979 1166 Size: 1452 361 4386 Fragments arranged by size: 4386 1452 361 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6199 AlwNI CAG_nnn'CTG Cuts at: 2956 3307 3942 5514 2956 Size: 351 635 1572 3641 Fragments arranged by size: 3641 1572 635 351 ApaI G_GGCC'C Cuts at: 2231 2262 2231 Size: 31 6168 ApaLI G'TGCA_C Cuts at: 2384 2720 3056 4256 5412 2384 Size: 336 336 1200 1156 3171 Fragments arranged by size: 3171 1200 1156 336 336 ApoI r'AATT_y Cuts at: 1493 3508 3734 3886 5995 1493 Size: 2015 226 152 2109 1697 Fragments arranged by size: 2109 2015 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4753 160 Size: 872 3721 1606 Fragments arranged by size: 3721 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 AvaI C'yCGr_G Cuts at: 1870 1943 2414 3145 3524 1870 Size: 73 471 731 379 4545 Fragments arranged by size: 4545 731 471 379 73 BamHI G'GATC_C Cuts at: 3529 3896 3529 Size: 367 5832 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3149 3520 4975 618 Size: 325 217 627 1362 371 1455 1842 Fragments arranged by size: 1842 1455 1362 627 371 325 217 BanII G_rGCy'C Cuts at: 1391 2231 2262 2360 2444 2780 2948 3032 3375 Size: 840 31 98 84 336 168 84 343 Cuts at: 3375 3518 1391 Size: 143 4072 Fragments arranged by size: 4072 840 343 336 168 143 98 84 84 31 BbeI G_GCGC'C Cuts at: 3153 3153 Size: 6199 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2078 928 Size: 971 179 5049 Fragments arranged by size: 5049 971 179 BbvCI CC'TCA_GC Cuts at: 2450 2450 Size: 6199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2413 2447 2581 2615 2833 2867 3337 Size: 34 476 34 134 34 218 34 470 Cuts at: 3337 3371 4390 4424 1903 Size: 34 1019 34 3678 Fragments arranged by size: 3678 1019 476 470 218 134 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4091 5301 4091 Size: 1210 4989 BclI T'GATC_A Cuts at: 1548 1548 Size: 6199 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4810 136 Size: 107 122 71 631 605 3138 1525 Fragments arranged by size: 3138 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6194 6194 Size: 6199 BlpI GC'TnA_GC Cuts at: 3616 3616 Size: 6199 Bme1580I G_kGCm'C Cuts at: 1790 2231 2262 2388 2414 2666 2724 3060 4260 Size: 441 31 126 26 252 58 336 1200 Cuts at: 4260 5416 1790 Size: 1156 2573 Fragments arranged by size: 2573 1200 1156 441 336 252 126 58 31 26 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6199 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2195 3082 3930 Size: 416 53 83 399 24 778 887 848 Cuts at: 3930 4888 442 Size: 958 1753 Fragments arranged by size: 1753 958 887 848 778 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2130 2458 2726 4859 1239 Size: 891 328 268 2133 2579 Fragments arranged by size: 2579 2133 891 328 268 Bpu10I CC'TnA_GC Cuts at: 2450 2450 Size: 6199 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4324 5189 5487 4324 Size: 865 298 5036 Fragments arranged by size: 5036 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4862 882 Size: 617 3363 2219 Fragments arranged by size: 3363 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2409 3990 493 Size: 711 1205 1581 2702 Fragments arranged by size: 2702 1581 1205 711 BsaBI GATnn'nnATC Cuts at: 6193 6193 Size: 6199 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3150 4006 4388 5023 Size: 53 83 186 936 1617 856 382 635 Cuts at: 5023 275 Size: 1451 Fragments arranged by size: 1617 1451 936 856 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4632 5030 5304 5451 5862 4632 Size: 398 274 147 411 4969 Fragments arranged by size: 4969 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5974 Fragments arranged by size: 5974 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 1186 Size: 1138 5061 BseYI C'CCAG_C Cuts at: 2115 2224 2255 5402 5898 2115 Size: 109 31 3147 496 2416 Fragments arranged by size: 3147 2416 496 109 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2642 3293 1163 Size: 573 906 651 4069 Fragments arranged by size: 4069 906 651 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2605 2738 3649 4410 5438 1540 Size: 275 186 604 133 911 761 1028 2301 Fragments arranged by size: 2301 1028 911 761 604 275 186 133 BsiHKAI G_wGCw'C Cuts at: 2388 2444 2712 2724 2780 2796 2948 3048 3060 Size: 56 268 12 56 16 152 100 12 Cuts at: 3060 3375 3468 4260 4345 5416 2388 Size: 315 93 792 85 1071 3171 Fragments arranged by size: 3171 1071 792 315 268 152 100 93 85 56 56 16 12 12 BsmI GAATG_Cn' Cuts at: 3263 3710 3803 3263 Size: 447 93 5659 Fragments arranged by size: 5659 447 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2231 2262 2360 2388 2414 2444 2666 Size: 399 441 31 98 28 26 30 222 Cuts at: 2666 2712 2724 2780 2796 2948 3032 3048 3060 Size: 46 12 56 16 152 84 16 12 Cuts at: 3060 3375 3468 3518 4260 4345 5416 1391 Size: 315 93 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 441 399 315 222 152 98 93 85 84 56 50 46 31 30 28 26 16 16 12 12 BspEI T'CCGG_A Cuts at: 5862 5862 Size: 6199 BspHI T'CATG_A Cuts at: 4088 5818 4088 Size: 1730 4469 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2023 3554 844 Size: 1179 1531 3489 Fragments arranged by size: 3489 1531 1179 BsrBI CCG'CTC Cuts at: 1394 1823 2986 4086 1394 Size: 429 1163 1100 3507 Fragments arranged by size: 3507 1163 1100 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2071 4694 4868 65 Size: 1183 823 2623 174 1396 Fragments arranged by size: 2623 1396 1183 823 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2322 2712 2880 3048 3132 3216 3384 Size: 110 512 390 168 168 84 84 168 Cuts at: 3384 4843 5030 1700 Size: 1459 187 2869 Fragments arranged by size: 2869 1459 512 390 187 168 168 168 110 84 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6199 BssHII G'CGCG_C Cuts at: 1925 2520 2730 1925 Size: 595 210 5394 Fragments arranged by size: 5394 595 210 BssSI C'ACGA_G Cuts at: 2080 4259 5271 2080 Size: 2179 1012 3008 Fragments arranged by size: 3008 2179 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2502 2956 794 Size: 1708 454 4037 Fragments arranged by size: 4037 1708 454 BstBI TT'CG_AA Cuts at: 5003 5003 Size: 6199 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6199 BstXI CCAn_nnnn'nTGG Cuts at: 2022 2704 2022 Size: 682 5517 BstYI r'GATC_y Cuts at: 1972 2802 3529 3896 4281 4298 5750 5836 5848 Size: 830 727 367 385 17 1452 86 12 Cuts at: 5848 6194 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 830 727 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 3190 3274 513 Size: 552 939 132 1054 84 3438 Fragments arranged by size: 3438 1054 939 552 132 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5460 Fragments arranged by size: 5460 683 56 BtsI GCAGTG_nn' Cuts at: 3340 3715 4509 4529 3340 Size: 375 794 20 5010 Fragments arranged by size: 5010 794 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6199 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6164 DraI TTT'AAA Cuts at: 3503 3503 Size: 6199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5206 809 Size: 507 3890 1802 Fragments arranged by size: 3890 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2164 2602 2735 Size: 54 1425 50 275 18 334 438 133 Cuts at: 2735 2787 2819 3010 3646 4535 6164 8 Size: 52 32 191 636 889 1629 43 Fragments arranged by size: 1629 1425 889 636 438 334 275 191 133 54 52 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2602 2735 3646 1537 Size: 275 790 133 911 4090 Fragments arranged by size: 4090 911 790 275 133 EarI CTCTTCn'nnn_ Cuts at: 1353 4129 1353 Size: 2776 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2210 3056 4786 5160 5306 387 Size: 1064 138 621 846 1730 374 146 1280 Fragments arranged by size: 1730 1280 1064 846 621 374 146 138 EcoICRI GAG'CTC Cuts at: 2442 2778 2946 3373 2442 Size: 336 168 427 5268 Fragments arranged by size: 5268 427 336 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2458 2585 2668 2726 2752 2819 Size: 393 498 328 127 83 58 26 67 Cuts at: 2819 3088 4262 4859 5646 5914 1239 Size: 269 1174 597 787 268 1524 Fragments arranged by size: 1524 1174 787 597 498 393 328 269 268 127 83 67 58 26 EcoNI CCTnn'n_nnAGG Cuts at: 2093 2093 Size: 6199 EcoO109I rG'GnC_Cy Cuts at: 2056 2086 2121 2210 2228 3408 2056 Size: 30 35 89 18 1180 4847 Fragments arranged by size: 4847 1180 89 35 30 18 EcoRI G'AATT_C Cuts at: 3508 3886 3508 Size: 378 5821 FspI TGC'GCA Cuts at: 3253 3362 4705 3253 Size: 109 1343 4747 Fragments arranged by size: 4747 1343 109 HaeII r_GCGC'y Cuts at: 1678 3115 3153 3307 5346 1678 Size: 1437 38 154 2039 2531 Fragments arranged by size: 2531 2039 1437 154 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4840 4872 4914 4946 1373 Size: 32 135 32 3268 32 42 32 2626 Fragments arranged by size: 3268 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3543 3789 669 Size: 988 281 1605 246 3079 Fragments arranged by size: 3079 1605 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6199 HpaI GTT'AAC Cuts at: 3789 3789 Size: 6199 KasI G'GCGC_C Cuts at: 3149 3149 Size: 6199 KpnI G_GTAC'C Cuts at: 3524 3524 Size: 6199 MfeI C'AATT_G Cuts at: 3798 3798 Size: 6199 MluI A'CGCG_T Cuts at: 6075 6075 Size: 6199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2469 2805 3141 3348 5313 5497 1305 Size: 1164 336 336 207 1965 184 2007 Fragments arranged by size: 2007 1965 1164 336 336 207 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4377 Fragments arranged by size: 4377 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2618 2979 4157 4516 4675 518 Size: 648 1452 361 1178 359 159 2042 Fragments arranged by size: 2042 1452 1178 648 361 359 159 MspA1I CmG'CkG Cuts at: 1606 2170 2475 2559 2577 2688 3192 3276 3615 Size: 564 305 84 18 111 504 84 339 Cuts at: 3615 4292 5440 5685 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 564 504 339 305 245 111 84 84 18 NarI GG'CG_CC Cuts at: 3150 3150 Size: 6199 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4708 Fragments arranged by size: 4708 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6199 NotI GC'GGCC_GC Cuts at: 2735 3646 2735 Size: 911 5288 NspI r_CATG'y Cuts at: 1132 1229 3557 5102 1132 Size: 97 2328 1545 2229 Fragments arranged by size: 2328 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6199 PciI A'CATG_T Cuts at: 5098 5098 Size: 6199 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6199 PfoI T'CCnGG_A Cuts at: 2703 2703 Size: 6199 PmeI GTTT'AAAC Cuts at: 3503 3503 Size: 6199 PmlI CAC'GTG Cuts at: 2409 2409 Size: 6199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3311 3343 4268 4300 5781 5813 3311 Size: 32 925 32 1481 32 3697 Fragments arranged by size: 3697 1481 925 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2056 Size: 6199 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6199 PsiI TTA'TAA Cuts at: 3769 3769 Size: 6199 PspOMI G'GGCC_C Cuts at: 2227 2258 2227 Size: 31 6168 PstI C_TGCA'G Cuts at: 830 2034 2278 2373 2961 3347 3551 830 Size: 1204 244 95 588 386 204 3478 Fragments arranged by size: 3478 1204 588 386 244 204 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6199 PvuII CAG'CTG Cuts at: 2688 2688 Size: 6199 SacI G_AGCT'C Cuts at: 2444 2780 2948 3375 2444 Size: 336 168 427 5268 Fragments arranged by size: 5268 427 336 168 SacII CC_GC'GG Cuts at: 3193 3277 3193 Size: 84 6115 SalI G'TCGA_C Cuts at: 1936 3541 1936 Size: 1605 4594 SbfI CC_TGCA'GG Cuts at: 2034 3551 2034 Size: 1517 4682 ScaI AGT'ACT Cuts at: 4447 6159 4447 Size: 1712 4487 SfcI C'TryA_G Cuts at: 826 1045 2030 2274 2369 2957 3343 3547 4682 Size: 219 985 244 95 588 386 204 1135 Cuts at: 4682 5363 5554 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 985 681 588 386 244 219 204 191 95 SfoI GGC'GCC Cuts at: 3151 3151 Size: 6199 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 SgrAI Cr'CCGG_yG Cuts at: 1700 2712 2880 3048 3132 3384 1700 Size: 1012 168 168 84 252 4515 Fragments arranged by size: 4515 1012 252 168 168 84 SmaI CCC'GGG Cuts at: 1872 3526 1872 Size: 1654 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3376 4303 5204 5466 820 Size: 197 926 1433 927 901 262 1553 Fragments arranged by size: 1553 1433 927 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3990 493 Size: 3497 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6199 SphI G_CATG'C Cuts at: 1132 3557 1132 Size: 2425 3774 SspI AAT'ATT Cuts at: 5 52 4123 5 Size: 47 4071 2081 Fragments arranged by size: 4071 2081 47 StuI AGG'CCT Cuts at: 1517 2429 2513 2681 2765 2849 3101 3185 3269 Size: 912 84 168 84 84 252 84 84 Cuts at: 3269 3353 1517 Size: 84 4363 Fragments arranged by size: 4363 912 252 168 84 84 84 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 2004 3638 513 Size: 552 939 1634 3074 Fragments arranged by size: 3074 1634 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4226 4243 4396 4581 5026 325 Size: 1255 2646 17 153 185 445 1498 Fragments arranged by size: 2646 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4445 6157 96 Size: 274 80 33 51 1443 2468 1712 138 Fragments arranged by size: 2468 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2128 2160 6159 6191 2128 Size: 32 3999 32 2136 Fragments arranged by size: 3999 2136 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6199 XbaI T'CTAG_A Cuts at: 3535 3535 Size: 6199 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6199 XmaI C'CCGG_G Cuts at: 1870 3524 1870 Size: 1654 4545 XmnI GAAnn'nnTTC Cuts at: 2570 2906 3437 4328 2570 Size: 336 531 891 4441 Fragments arranged by size: 4441 891 531 336 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4007 276 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI