Restriction map for
pFN21AB9666
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbeI BciVI
BclI BfaI BfrI BglII BlnI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MaeIII MamI MfeI MluI MroI
MseI MunI NarI NcoI NdeI
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tsp45I
Tsp509I Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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Aor51HI 819 AGCGCT AGC^GCT
AvaI 997 CTCGGG C^[CT]CG[GA]G
BalI 140 TGGCCA TGG^CCA
BamHI 1155 GGATCC G^GATCC
BbrPI 838 CACGTG CAC^GTG
BbsI 3 GTCTTC GAAGAC(2/6)
BglI 313 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3 GTCTTC GAAGAC(2/6)
BsaAI 838 CACGTG [CT]AC^GT[GA]
BsaI 1041 GGTCTC GGTCTC(1/5)
BseRI 108 CTCCTC GAGGAG(10/8)
BsmAI 1042 GTCTC GTCTC(1/5)
BsmI 843 GCATTC GAATGC(1/-1)
BsoBI 997 CTCGGG C^[CT]CG[GA]G
BssSI 1358 CTCGTG CACGAG(-5/-1)
BstAPI 937 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 819 AGCGCT AGC^GCT
HphI 908 TCACC GGTGA(8/7)
MaeII 839 ACGT A^CGT
MluNI 140 TGGCCA TGG^CCA
MscI 140 TGGCCA TGG^CCA
MslI 928 CATGAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 985 GCCGGC GCC^GGC
NgoMIV 985 GCCGGC G^CCGGC
PleI 1395 GAGTC GAGTC(4/5)
PmaCI 838 CACGTG CAC^GTG
PmlI 838 CACGTG CAC^GTG
PpuMI 1053 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1240 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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Asp700I 294 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
882 GAAGGCCTTC
BanI 310 GGTGCC G^G[CT][GA]CC
1102 GGCACC
BbvCI 75 CCTCAGC CCTCAGC(-5/-2)
1092 CCTCAGC
BsgI 835 CTGCAC GTGCAG(16/14)
935 GTGCAG
BsmFI 857 GTCCC GGGAC(10/14)
1247 GTCCC
BsrBI 601 GAGCGG CCGCTC(-3/-3)
769 GAGCGG
BsrFI 985 GCCGGC [GA]^CCGG[CT]
1387 ACCGGC
BsrI 343 ACTGG ACTGG(1/-1)
360 CCAGT
BstYI 1155 GGATCC [GA]^GATC[CT]
1186 AGATCC
Cfr10I 985 GCCGGC [GA]^CCGG[CT]
1387 ACCGGC
DraII 1053 GGGTCCT [GA]G^G[ACGT]CC[CT]
1431 GGGGCCT
EcoO109I 1053 GGGTCCT [GA]G^G[ACGT]CC[CT]
1431 GGGGCCT
EcoT14I 545 CCAAGG C^C[AT][AT]GG
797 CCAAGG
HgaI 1240 GACGC GACGC(5/10)
855 GCGTC
MboII 4 TCTTC GAAGA(8/7)
1216 TCTTC
MflI 1155 GGATCC [GA]^GATC[CT]
1186 AGATCC
NlaIII 928 CATG CATG^
1023 CATG
NotI 958 GCGGCCGC GC^GGCCGC
979 GCGGCCGC
PflMI 132 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1225 CCAAAGGCTGG
PvuII 950 CAGCTG CAG^CTG
968 CAGCTG
StyI 545 CCAAGG C^C[AT][AT]GG
797 CCAAGG
TfiI 51 GATTC G^A[AT]TC
1213 GAATC
Van91I 132 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1225 CCAAAGGCTGG
XhoII 1155 GGATCC [GA]^GATC[CT]
1186 AGATCC
XmnI 294 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
882 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII AviII BanII
BbvI BcgI BcnI BlpI BmyI
BpmI Bpu1102I BsaJI BsiEI BsiHKAI
BsiYI BslI Bsp1286I BspMI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DpnII DsaI
EaeI EagI EclXI Eco52I EcoRII
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI KspI MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV PstI RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TaqI TseI
TspRI TthHB8I