(Circular) (Six-base) MAPSORT of: pFN21AB9666.seq Check: 8076 from: 1 to: 6148 pFN21AB9666 6148 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2511 2595 2763 2847 2511 Size: 84 168 84 5812 Fragments arranged by size: 5812 168 84 84 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3491 1937 Size: 1554 4594 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2290 4211 5595 5863 1632 Size: 658 1921 1384 268 1917 Fragments arranged by size: 1921 1917 1384 658 268 AfeI AGC'GCT Cuts at: 2826 2826 Size: 6148 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 5047 6024 1203 Size: 3844 977 1327 Fragments arranged by size: 3844 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6148 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2955 1184 Size: 1771 4377 AlwNI CAG_nnn'CTG Cuts at: 2503 2839 2948 3302 3891 5463 2503 Size: 336 109 354 589 1572 3188 Fragments arranged by size: 3188 1572 589 354 336 109 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 3457 3683 3835 5944 1493 Size: 1964 226 152 2109 1697 Fragments arranged by size: 2109 1964 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 3002 3473 1870 Size: 73 1059 471 4545 Fragments arranged by size: 4545 1059 471 73 BamHI G'GATC_C Cuts at: 3160 3478 3845 3160 Size: 318 367 5463 Fragments arranged by size: 5463 367 318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2315 3107 3469 4924 618 Size: 325 217 627 528 792 362 1455 1842 Fragments arranged by size: 1842 1455 792 627 528 362 325 217 BanII G_rGCy'C Cuts at: 1391 2570 2654 2738 2822 3467 1391 Size: 1179 84 84 84 645 4072 Fragments arranged by size: 4072 1179 645 84 84 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 928 Size: 971 102 5075 Fragments arranged by size: 5075 971 102 BbvCI CC'TCA_GC Cuts at: 2081 3098 2081 Size: 1017 5131 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2371 2405 2455 2489 2623 2657 2707 Size: 34 434 34 50 34 134 34 50 Cuts at: 2707 2741 4339 4373 1903 Size: 34 1598 34 3678 Fragments arranged by size: 3678 1598 434 134 50 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4040 5250 4040 Size: 1210 4938 BclI T'GATC_A Cuts at: 1548 1548 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2324 4759 136 Size: 107 122 71 631 605 652 2435 1525 Fragments arranged by size: 2435 1525 652 631 605 122 107 71 BglII A'GATC_T Cuts at: 6143 6143 Size: 6148 BlpI GC'TnA_GC Cuts at: 2240 2328 2821 3565 2240 Size: 88 493 744 4823 Fragments arranged by size: 4823 744 493 88 Bme1580I G_kGCm'C Cuts at: 1790 2481 2540 3152 3243 3267 3291 3315 3430 Size: 691 59 612 91 24 24 24 115 Cuts at: 3430 4209 5365 1790 Size: 779 1156 2573 Fragments arranged by size: 2573 1156 779 691 612 115 91 59 24 24 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2358 3879 4837 Size: 416 53 83 399 24 941 1521 958 Cuts at: 4837 442 Size: 1753 Fragments arranged by size: 1753 1521 958 941 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2816 2890 3172 4808 1239 Size: 1577 74 282 1636 2579 Fragments arranged by size: 2579 1636 1577 282 74 Bpu10I CC'TnA_GC Cuts at: 2081 3014 3098 2081 Size: 933 84 5131 Fragments arranged by size: 5131 933 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4273 5138 5436 4273 Size: 865 298 4985 Fragments arranged by size: 4985 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3052 4811 882 Size: 617 1553 1759 2219 Fragments arranged by size: 2219 1759 1553 617 BsaAI yAC'GTr Cuts at: 493 1204 2845 3939 493 Size: 711 1641 1094 2702 Fragments arranged by size: 2702 1641 1094 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3955 4337 4972 275 Size: 53 83 186 936 2422 382 635 1451 Fragments arranged by size: 2422 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4581 4979 5253 5400 5811 4581 Size: 398 274 147 411 4918 Fragments arranged by size: 4918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 1178 Size: 30 165 30 693 30 5200 Fragments arranged by size: 5200 693 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2104 1186 Size: 918 5230 BseYI C'CCAG_C Cuts at: 3164 3200 3236 3266 3290 3314 3379 3433 5351 Size: 36 36 30 24 24 65 54 1918 Cuts at: 5351 5847 3164 Size: 496 3465 Fragments arranged by size: 3465 1918 496 65 54 36 36 30 24 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2825 2961 1163 Size: 573 1089 136 4350 Fragments arranged by size: 4350 1089 573 136 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2360 2688 2967 2988 3598 4359 Size: 275 186 359 328 279 21 610 761 Cuts at: 4359 5387 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 610 359 328 279 275 186 21 BsiHKAI G_wGCw'C Cuts at: 2166 2654 2738 2822 3081 4209 4294 5365 2166 Size: 488 84 84 259 1128 85 1071 2949 Fragments arranged by size: 2949 1128 1071 488 259 85 84 84 BsmI GAATG_Cn' Cuts at: 2848 3659 3752 2848 Size: 811 93 5244 Fragments arranged by size: 5244 811 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2481 2540 2570 2654 2738 2822 Size: 399 376 315 59 30 84 84 84 Cuts at: 2822 3081 3152 3243 3267 3291 3315 3430 3467 Size: 259 71 91 24 24 24 115 37 Cuts at: 3467 4209 4294 5365 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 376 315 259 115 91 85 84 84 84 71 59 37 30 24 24 24 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 4037 5767 4037 Size: 1730 4418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2511 2595 2763 2847 3034 3503 844 Size: 1667 84 168 84 187 469 3489 Fragments arranged by size: 3489 1667 469 187 168 84 84 BsrBI CCG'CTC Cuts at: 1394 1823 2608 2776 4035 1394 Size: 429 785 168 1259 3507 Fragments arranged by size: 3507 1259 785 429 168 BsrDI GCAATG_nn' Cuts at: 65 1248 4643 4817 65 Size: 1183 3395 174 1396 Fragments arranged by size: 3395 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2990 3392 4792 4979 1700 Size: 110 1180 402 1400 187 2869 Fragments arranged by size: 2869 1400 1180 402 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 3363 4208 5220 3363 Size: 845 1012 4291 Fragments arranged by size: 4291 1012 845 BstAPI GCAn_nnn'nTGC Cuts at: 794 2948 794 Size: 2154 3994 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6148 BstYI r'GATC_y Cuts at: 1972 3160 3191 3478 3845 4230 4247 5699 5785 Size: 1188 31 287 367 385 17 1452 86 Cuts at: 5785 5797 6143 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1188 385 367 346 287 86 31 17 12 BtgI C'CryG_G Cuts at: 513 1065 2157 2311 2335 2522 2961 513 Size: 552 1092 154 24 187 439 3700 Fragments arranged by size: 3700 1092 552 439 187 154 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2234 2402 487 Size: 683 56 1008 168 4233 Fragments arranged by size: 4233 1008 683 168 56 BtsI GCAGTG_nn' Cuts at: 2124 2449 2633 2710 2794 3428 3664 4458 4478 Size: 325 184 77 84 634 236 794 20 Cuts at: 4478 2124 Size: 3794 Fragments arranged by size: 3794 794 634 325 236 184 84 77 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5155 809 Size: 507 3839 1802 Fragments arranged by size: 3839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2357 2560 Size: 54 1425 50 275 18 315 212 203 Cuts at: 2560 2685 2812 2896 2964 2985 3118 3595 4484 Size: 125 127 84 68 21 133 477 889 Cuts at: 4484 6113 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 477 315 275 212 203 133 127 125 84 68 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2357 2685 2964 2985 3595 1537 Size: 275 545 328 279 21 610 4090 Fragments arranged by size: 4090 610 545 328 279 275 21 EarI CTCTTCn'nnn_ Cuts at: 1353 4078 1353 Size: 2725 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2213 4735 5109 5255 387 Size: 1064 138 624 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 624 374 146 138 EcoICRI GAG'CTC Cuts at: 2652 2736 2820 2652 Size: 84 84 5980 Fragments arranged by size: 5980 84 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2290 2816 2890 3172 4211 4808 5595 Size: 393 658 526 74 282 1039 597 787 Cuts at: 5595 5863 1239 Size: 268 1524 Fragments arranged by size: 1524 1039 787 658 597 526 393 282 268 74 EcoO109I rG'GnC_Cy Cuts at: 3059 3437 3059 Size: 378 5770 EcoRI G'AATT_C Cuts at: 3457 3835 3457 Size: 378 5770 FspI TGC'GCA Cuts at: 2507 2591 2759 4654 2507 Size: 84 168 1895 4001 Fragments arranged by size: 4001 1895 168 84 HaeII r_GCGC'y Cuts at: 1678 2401 2660 2695 2828 5295 1678 Size: 723 259 35 133 2467 2531 Fragments arranged by size: 2531 2467 723 259 133 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2047 2079 2096 2128 2233 Size: 32 135 32 475 32 17 32 105 Cuts at: 2233 2265 3122 3152 3154 3184 4789 4821 4863 Size: 32 857 30 2 30 1605 32 42 Cuts at: 4863 4895 1373 Size: 32 2626 Fragments arranged by size: 2626 1605 857 475 135 105 42 32 32 32 32 32 32 32 30 30 17 2 HincII GTy'rAC Cuts at: 669 1657 1938 3492 3738 669 Size: 988 281 1554 246 3079 Fragments arranged by size: 3079 1554 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2763 5262 5446 1305 Size: 1458 2499 184 2007 Fragments arranged by size: 2499 2007 1458 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 10 Size: 54 1425 343 315 4011 Fragments arranged by size: 4011 1425 343 315 54 MslI CAynn'nnrTG Cuts at: 518 1166 2937 4106 4465 4624 518 Size: 648 1771 1169 359 159 2042 Fragments arranged by size: 2042 1771 1169 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2159 2265 2283 2433 2524 2703 2948 2957 Size: 553 106 18 150 91 179 245 9 Cuts at: 2957 2963 2969 2975 2984 3564 4241 5389 5634 Size: 6 6 6 9 580 677 1148 245 Cuts at: 5634 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 580 553 245 245 179 150 106 91 18 9 9 6 6 6 NaeI GCC'GGC Cuts at: 2992 2992 Size: 6148 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5596 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NgoMIV G'CCGG_C Cuts at: 2990 2990 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 2964 2985 3595 2964 Size: 21 610 5517 Fragments arranged by size: 5517 610 21 NspI r_CATG'y Cuts at: 1132 1229 3506 5051 1132 Size: 97 2277 1545 2229 Fragments arranged by size: 2277 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 2143 3236 1108 Size: 1035 1093 4020 Fragments arranged by size: 4020 1093 1035 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PmlI CAC'GTG Cuts at: 2845 2845 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3059 3059 Size: 6148 PshAI GACnn'nnGTC Cuts at: 1613 3249 1613 Size: 1636 4512 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PstI C_TGCA'G Cuts at: 830 2667 2956 2974 2980 3500 830 Size: 1837 289 18 6 520 3478 Fragments arranged by size: 3478 1837 520 289 18 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2957 2975 2957 Size: 18 6130 SacI G_AGCT'C Cuts at: 2654 2738 2822 2654 Size: 84 84 5980 Fragments arranged by size: 5980 84 84 SacII CC_GC'GG Cuts at: 2160 2525 2964 2160 Size: 365 439 5344 Fragments arranged by size: 5344 439 365 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 4396 6108 4396 Size: 1712 4436 SfcI C'TryA_G Cuts at: 826 1045 2663 2952 2970 2976 3496 4631 5312 Size: 219 1618 289 18 6 520 1135 681 Cuts at: 5312 5503 826 Size: 191 1471 Fragments arranged by size: 1618 1471 1135 681 520 289 219 191 18 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4252 5153 5415 820 Size: 197 926 2309 901 262 1553 Fragments arranged by size: 2309 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 4072 5 Size: 47 4020 2081 Fragments arranged by size: 4020 2081 47 StuI AGG'CCT Cuts at: 1517 2387 2471 2639 2807 2891 2903 1517 Size: 870 84 168 168 84 12 4762 Fragments arranged by size: 4762 870 168 168 84 84 12 StyI C'CwwG_G Cuts at: 513 1065 2550 2802 3587 513 Size: 552 1485 252 785 3074 Fragments arranged by size: 3074 1485 785 552 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4175 4192 4345 4530 4975 325 Size: 1255 2595 17 153 185 445 1498 Fragments arranged by size: 2595 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4394 6106 96 Size: 274 80 33 51 1443 2417 1712 138 Fragments arranged by size: 2417 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6108 6140 6108 Size: 32 6116 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 2303 2891 4277 2303 Size: 588 1386 4174 Fragments arranged by size: 4174 1386 588 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI