Restriction map for
pFN21AB9666
(Linear) (Six-base) MAPSORT of: KIBB9666.seq Check: 7120 from: 1 to: 1443
KIBB9666.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 506 590 758 842 1443
Size: 506 84 168 84 601
Fragments arranged by size:
601 506 168 84 84
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 1443
Size: 285 1158
AfeI AGC'GCT
Cuts at: 0 821 1443
Size: 821 622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 950 1443
Size: 950 493
AlwNI CAG_nnn'CTG
Cuts at: 0 498 834 943 1297 1443
Size: 498 336 109 354 146
Fragments arranged by size:
498 354 336 146 109
AvaI C'yCGr_G
Cuts at: 0 997 1443
Size: 997 446
BamHI G'GATC_C
Cuts at: 0 1155 1443
Size: 1155 288
BanI G'GyrC_C
Cuts at: 0 310 1102 1443
Size: 310 792 341
Fragments arranged by size:
792 341 310
BanII G_rGCy'C
Cuts at: 0 565 649 733 817 1443
Size: 565 84 84 84 626
Fragments arranged by size:
626 565 84 84 84
BbvCI CC'TCA_GC
Cuts at: 0 76 1093 1443
Size: 76 1017 350
Fragments arranged by size:
1017 350 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 366 400 450 484 618 652 702 736
Size: 366 34 50 34 134 34 50 34
Cuts at: 736 1443
Size: 707
Fragments arranged by size:
707 366 134 50 50 34 34 34
34
BglI GCCn_nnn'nGGC
Cuts at: 0 319 1443
Size: 319 1124
BlpI GC'TnA_GC
Cuts at: 0 235 323 816 1443
Size: 235 88 493 627
Fragments arranged by size:
627 493 235 88
Bme1580I G_kGCm'C
Cuts at: 0 476 535 1147 1238 1262 1286 1310 1425
Size: 476 59 612 91 24 24 24 115
Cuts at: 1425 1443
Size: 18
Fragments arranged by size:
612 476 115 91 59 24 24 24
18
BmrI ACTGGGnnnn_n'
Cuts at: 0 353 1443
Size: 353 1090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 885 1167 1443
Size: 811 74 282 276
Fragments arranged by size:
811 282 276 74
Bpu10I CC'TnA_GC
Cuts at: 0 76 1009 1093 1443
Size: 76 933 84 350
Fragments arranged by size:
933 350 84 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 1047 1443
Size: 1047 396
BsaAI yAC'GTr
Cuts at: 0 840 1443
Size: 840 603
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 1443
Size: 91 30 1322
Fragments arranged by size:
1322 91 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 99 1443
Size: 99 1344
BseYI C'CCAG_C
Cuts at: 0 1159 1195 1231 1261 1285 1309 1374 1428
Size: 1159 36 36 30 24 24 65 54
Cuts at: 1428 1443
Size: 15
Fragments arranged by size:
1159 65 54 36 36 30 24 24
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 956 1443
Size: 820 136 487
Fragments arranged by size:
820 487 136
BsiEI CG_ry'CG
Cuts at: 0 355 683 962 983 1443
Size: 355 328 279 21 460
Fragments arranged by size:
460 355 328 279 21
BsiHKAI G_wGCw'C
Cuts at: 0 161 649 733 817 1076 1443
Size: 161 488 84 84 259 367
Fragments arranged by size:
488 367 259 161 84 84
BsmI GAATG_Cn'
Cuts at: 0 843 1443
Size: 843 600
Bsp1286I G_dGCh'C
Cuts at: 0 161 476 535 565 649 733 817 1076
Size: 161 315 59 30 84 84 84 259
Cuts at: 1076 1147 1238 1262 1286 1310 1425 1443
Size: 71 91 24 24 24 115 18
Fragments arranged by size:
315 259 161 115 91 84 84 84
71 59 30 24 24 24 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 506 590 758 842 1029 1443
Size: 506 84 168 84 187 414
Fragments arranged by size:
506 414 187 168 84 84
BsrBI CCG'CTC
Cuts at: 0 603 771 1443
Size: 603 168 672
Fragments arranged by size:
672 603 168
BsrFI r'CCGG_y
Cuts at: 0 985 1387 1443
Size: 985 402 56
Fragments arranged by size:
985 402 56
BssSI C'ACGA_G
Cuts at: 0 1358 1443
Size: 1358 85
BstAPI GCAn_nnn'nTGC
Cuts at: 0 943 1443
Size: 943 500
BstYI r'GATC_y
Cuts at: 0 1155 1186 1443
Size: 1155 31 257
Fragments arranged by size:
1155 257 31
BtgI C'CryG_G
Cuts at: 0 152 306 330 517 956 1443
Size: 152 154 24 187 439 487
Fragments arranged by size:
487 439 187 154 152 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 229 397 1443
Size: 229 168 1046
Fragments arranged by size:
1046 229 168
BtsI GCAGTG_nn'
Cuts at: 0 119 444 628 705 789 1423 1443
Size: 119 325 184 77 84 634 20
Fragments arranged by size:
634 325 184 119 84 77 20
EaeI y'GGCC_r
Cuts at: 0 140 352 555 680 807 891 959 980
Size: 140 212 203 125 127 84 68 21
Cuts at: 980 1113 1443
Size: 133 330
Fragments arranged by size:
330 212 203 140 133 127 125 84
68 21
EagI C'GGCC_G
Cuts at: 0 352 680 959 980 1443
Size: 352 328 279 21 463
Fragments arranged by size:
463 352 328 279 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 208 1443
Size: 208 1235
EcoICRI GAG'CTC
Cuts at: 0 647 731 815 1443
Size: 647 84 84 628
Fragments arranged by size:
647 628 84 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 811 885 1167 1443
Size: 285 526 74 282 276
Fragments arranged by size:
526 285 282 276 74
EcoO109I rG'GnC_Cy
Cuts at: 0 1054 1432 1443
Size: 1054 378 11
Fragments arranged by size:
1054 378 11
FspI TGC'GCA
Cuts at: 0 502 586 754 1443
Size: 502 84 168 689
Fragments arranged by size:
689 502 168 84
HaeII r_GCGC'y
Cuts at: 0 396 655 690 823 1443
Size: 396 259 35 133 620
Fragments arranged by size:
620 396 259 133 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 74 91 123 228 260 1117 1147
Size: 42 32 17 32 105 32 857 30
Cuts at: 1147 1149 1179 1443
Size: 2 30 264
Fragments arranged by size:
857 264 105 42 32 32 32 30
30 17 2
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1443
Size: 758 685
MscI TGG'CCA
Cuts at: 0 142 1443
Size: 142 1301
MslI CAynn'nnrTG
Cuts at: 0 932 1443
Size: 932 511
MspA1I CmG'CkG
Cuts at: 0 154 260 278 428 519 698 943 952
Size: 154 106 18 150 91 179 245 9
Cuts at: 952 958 964 970 979 1443
Size: 6 6 6 9 464
Fragments arranged by size:
464 245 179 154 150 106 91 18
9 9 6 6 6
NaeI GCC'GGC
Cuts at: 0 987 1443
Size: 987 456
NgoMIV G'CCGG_C
Cuts at: 0 985 1443
Size: 985 458
NotI GC'GGCC_GC
Cuts at: 0 959 980 1443
Size: 959 21 463
Fragments arranged by size:
959 463 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 138 1231 1443
Size: 138 1093 212
Fragments arranged by size:
1093 212 138
PmlI CAC'GTG
Cuts at: 0 840 1443
Size: 840 603
PpuMI rG'GwC_Cy
Cuts at: 0 1054 1443
Size: 1054 389
PshAI GACnn'nnGTC
Cuts at: 0 1244 1443
Size: 1244 199
PstI C_TGCA'G
Cuts at: 0 662 951 969 975 1443
Size: 662 289 18 6 468
Fragments arranged by size:
662 468 289 18 6
PvuII CAG'CTG
Cuts at: 0 952 970 1443
Size: 952 18 473
Fragments arranged by size:
952 473 18
SacI G_AGCT'C
Cuts at: 0 649 733 817 1443
Size: 649 84 84 626
Fragments arranged by size:
649 626 84 84
SacII CC_GC'GG
Cuts at: 0 155 520 959 1443
Size: 155 365 439 484
Fragments arranged by size:
484 439 365 155
SfcI C'TryA_G
Cuts at: 0 658 947 965 971 1443
Size: 658 289 18 6 472
Fragments arranged by size:
658 472 289 18 6
StuI AGG'CCT
Cuts at: 0 382 466 634 802 886 898 1443
Size: 382 84 168 168 84 12 545
Fragments arranged by size:
545 382 168 168 84 84 12
StyI C'CwwG_G
Cuts at: 0 545 797 1443
Size: 545 252 646
Fragments arranged by size:
646 545 252
XmnI GAAnn'nnTTC
Cuts at: 0 298 886 1443
Size: 298 588 557
Fragments arranged by size:
588 557 298
Enzymes that do cut:
AarI AcuI AfeI AlfI AlwNI AvaI BamHI BanI
BanII BbvCI BcgI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI
BstYI BtgI BtgZI BtsI EaeI EagI EciI EcoICRI
Eco57MI EcoO109I FspI HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NgoMIV NotI PflMI PmlI PpuMI PshAI
PstI PvuII SacI SacII SfcI StuI StyI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BpuEI BsaBI BsaHI BsaWI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EarI EcoNI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI