(Circular) (Six-base) MAPSORT of: pFN21AB9662.seq Check: 1933 from: 1 to: 5716 pFN21AB9662 5716 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3059 1937 Size: 1122 4594 Acc65I G'GTAC_C Cuts at: 2469 3037 2469 Size: 568 5148 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2495 2930 3779 5163 5431 1632 Size: 863 435 849 1384 268 1917 Fragments arranged by size: 1917 1384 863 849 435 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 2487 4615 5592 1203 Size: 1284 2128 977 1327 Fragments arranged by size: 2128 1327 1284 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 3459 5031 3459 Size: 1572 4144 ApaI G_GGCC'C Cuts at: 2531 2531 Size: 5716 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 2639 3025 3251 3403 5512 1493 Size: 1146 386 226 152 2109 1697 Fragments arranged by size: 2109 1697 1146 386 226 152 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 2126 2202 2509 2669 3041 1870 Size: 73 183 76 307 160 372 4545 Fragments arranged by size: 4545 372 307 183 160 76 73 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2191 2260 2469 3037 4492 Size: 325 217 627 404 69 209 568 1455 Cuts at: 4492 618 Size: 1842 Fragments arranged by size: 1842 1455 627 568 404 325 217 209 69 BanII G_rGCy'C Cuts at: 1391 2531 3035 1391 Size: 1140 504 4072 Fragments arranged by size: 4072 1140 504 BbeI G_GCGC'C Cuts at: 2264 2264 Size: 5716 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2991 928 Size: 971 1092 3653 Fragments arranged by size: 3653 1092 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2314 4327 136 Size: 107 122 71 631 605 642 2013 1525 Fragments arranged by size: 2013 1525 642 631 605 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 2175 3133 2175 Size: 958 4758 Bme1580I G_kGCm'C Cuts at: 1790 2531 3777 4933 1790 Size: 741 1246 1156 2573 Fragments arranged by size: 2573 1246 1156 741 BmgBI CAC'GTC Cuts at: 1318 2278 2490 1318 Size: 960 212 4544 Fragments arranged by size: 4544 960 212 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2795 3447 4405 Size: 416 53 83 399 24 1378 652 958 Cuts at: 4405 442 Size: 1753 Fragments arranged by size: 1753 1378 958 652 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2007 2867 4376 1239 Size: 768 860 1509 2579 Fragments arranged by size: 2579 1509 860 768 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2372 3841 4706 5004 2372 Size: 1469 865 298 3084 Fragments arranged by size: 3084 1469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4379 882 Size: 617 2880 2219 Fragments arranged by size: 2880 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2537 3507 493 Size: 711 1333 970 2702 Fragments arranged by size: 2702 1333 970 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2261 3523 3905 4540 Size: 53 83 186 936 728 1262 382 635 Cuts at: 4540 275 Size: 1451 Fragments arranged by size: 1451 1262 936 728 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4149 4547 4821 4968 5379 4149 Size: 398 274 147 411 4486 Fragments arranged by size: 4486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5491 Fragments arranged by size: 5491 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2494 2497 1186 Size: 1081 227 3 4405 Fragments arranged by size: 4405 1081 227 3 BseYI C'CCAG_C Cuts at: 2568 4919 5415 2568 Size: 2351 496 2869 Fragments arranged by size: 2869 2351 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2413 2836 1163 Size: 573 677 423 4043 Fragments arranged by size: 4043 677 573 423 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 2653 3166 3927 4955 1540 Size: 275 186 36 616 513 761 1028 2301 Fragments arranged by size: 2301 1028 761 616 513 275 186 36 BsiHKAI G_wGCw'C Cuts at: 2133 2370 2433 2853 3777 3862 4933 2133 Size: 237 63 420 924 85 1071 2916 Fragments arranged by size: 2916 1071 924 420 237 85 63 BsmI GAATG_Cn' Cuts at: 3227 3320 3227 Size: 93 5623 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2370 2433 2531 2853 3035 3777 Size: 399 343 237 63 98 322 182 742 Cuts at: 3777 3862 4933 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 343 322 237 182 98 85 63 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3071 844 Size: 2227 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2298 3603 1394 Size: 429 475 1305 3507 Fragments arranged by size: 3507 1305 475 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4211 4385 65 Size: 1183 2963 174 1396 Fragments arranged by size: 2963 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2894 4360 4547 1700 Size: 110 1084 1466 187 2869 Fragments arranged by size: 2869 1466 1084 187 110 BsrGI T'GTAC_A Cuts at: 96 2283 96 Size: 2187 3529 BssHII G'CGCG_C Cuts at: 1925 2554 2841 1925 Size: 629 287 4800 Fragments arranged by size: 4800 629 287 BssSI C'ACGA_G Cuts at: 3776 4788 3776 Size: 1012 4704 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5716 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstYI r'GATC_y Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711 Size: 1074 367 385 17 1452 86 12 346 Cuts at: 5711 1972 Size: 1977 Fragments arranged by size: 1977 1452 1074 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2465 2465 Size: 5716 BtgI C'CryG_G Cuts at: 513 1065 2004 2183 2493 3009 513 Size: 552 939 179 310 516 3220 Fragments arranged by size: 3220 939 552 516 310 179 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 2414 3232 4026 4046 2414 Size: 818 794 20 4084 Fragments arranged by size: 4084 818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 3020 3020 Size: 5716 DraIII CAC_nnn'GTG Cuts at: 2814 2814 Size: 5716 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 3163 4052 Size: 54 1425 50 275 18 204 1129 889 Cuts at: 4052 5681 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1129 889 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2034 3163 1537 Size: 275 222 1129 4090 Fragments arranged by size: 4090 1129 275 222 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2691 2745 3646 1353 Size: 719 619 54 901 3423 Fragments arranged by size: 3423 901 719 619 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4303 4677 4823 387 Size: 1064 138 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2495 2867 2930 3779 4376 5163 Size: 393 375 488 372 63 849 597 787 Cuts at: 5163 5431 1239 Size: 268 1524 Fragments arranged by size: 1524 849 787 597 488 393 375 372 268 63 EcoNI CCTnn'n_nnAGG Cuts at: 2022 2022 Size: 5716 EcoO109I rG'GnC_Cy Cuts at: 2199 2206 2527 2528 2199 Size: 7 321 1 5387 Fragments arranged by size: 5387 321 7 1 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 2015 2113 2264 4863 1678 Size: 337 98 151 2599 2531 Fragments arranged by size: 2599 2531 337 151 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4357 4389 4431 4463 1373 Size: 32 135 32 2785 32 42 32 2626 Fragments arranged by size: 2785 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3306 669 Size: 988 281 1122 246 3079 Fragments arranged by size: 3079 1122 988 281 246 HindIII A'AGCT_T Cuts at: 748 2942 748 Size: 2194 3522 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KasI G'GCGC_C Cuts at: 2260 2260 Size: 5716 KpnI G_GTAC'C Cuts at: 2473 3041 2473 Size: 568 5148 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4830 5014 1305 Size: 3525 184 2007 Fragments arranged by size: 3525 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3894 Fragments arranged by size: 3894 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2767 3674 4033 4192 518 Size: 648 1326 275 907 359 159 2042 Fragments arranged by size: 2042 1326 907 648 359 275 159 MspA1I CmG'CkG Cuts at: 1606 2151 2185 2217 2339 2401 2634 3132 3809 Size: 545 34 32 122 62 233 498 677 Cuts at: 3809 4957 5202 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 545 498 245 233 122 62 34 32 NarI GG'CG_CC Cuts at: 2261 2261 Size: 5716 NcoI C'CATG_G Cuts at: 513 1065 2004 2493 3009 513 Size: 552 939 489 516 3220 Fragments arranged by size: 3220 939 552 516 489 NdeI CA'TA_TG Cuts at: 387 2777 387 Size: 2390 3326 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5716 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 3074 4619 1132 Size: 97 1845 1545 2229 Fragments arranged by size: 2229 1845 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5716 PciI A'CATG_T Cuts at: 4615 4615 Size: 5716 PflMI CCAn_nnn'nTGG Cuts at: 1108 2737 1108 Size: 1629 4087 PfoI T'CCnGG_A Cuts at: 2023 2406 2023 Size: 383 5333 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PspOMI G'GGCC_C Cuts at: 2527 2527 Size: 5716 PstI C_TGCA'G Cuts at: 830 2098 2956 3068 830 Size: 1268 858 112 3478 Fragments arranged by size: 3478 1268 858 112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 PvuII CAG'CTG Cuts at: 2217 2634 2217 Size: 417 5299 SacII CC_GC'GG Cuts at: 2186 2186 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SexAI A'CCwGG_T Cuts at: 2049 2049 Size: 5716 SfcI C'TryA_G Cuts at: 826 1045 2094 2952 3064 4199 4880 5071 826 Size: 219 1049 858 112 1135 681 191 1471 Fragments arranged by size: 1471 1135 1049 858 681 219 191 112 SfoI GGC'GCC Cuts at: 2262 2262 Size: 5716 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 2204 3043 1872 Size: 332 839 4545 Fragments arranged by size: 4545 839 332 SmlI C'TyrA_G Cuts at: 820 1017 1943 2387 3820 4721 4983 820 Size: 197 926 444 1433 901 262 1553 Fragments arranged by size: 1553 1433 926 901 444 262 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 2100 2789 1517 Size: 583 689 4444 Fragments arranged by size: 4444 689 583 StyI C'CwwG_G Cuts at: 513 1065 2004 2481 2493 3009 3155 513 Size: 552 939 477 12 516 146 3074 Fragments arranged by size: 3074 939 552 516 477 146 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3743 3760 3913 4098 4543 325 Size: 1255 2163 17 153 185 445 1498 Fragments arranged by size: 2163 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 3962 5674 Size: 274 80 33 51 1443 306 1679 1712 Cuts at: 5674 96 Size: 138 Fragments arranged by size: 1712 1679 1443 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2639 2671 5676 5708 2639 Size: 32 3005 32 2647 Fragments arranged by size: 3005 2647 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2923 2923 Size: 5716 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 2202 3041 1870 Size: 332 839 4545 Fragments arranged by size: 4545 839 332 XmnI GAAnn'nnTTC Cuts at: 3845 3845 Size: 5716 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI