(Circular) (Six-base) MAPSORT of: pFN21AB9661.seq Check: 4709 from: 1 to: 5734 pFN21AB9661 5734 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 3055 3055 Size: 5734 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3797 5181 5449 1632 Size: 2165 1384 268 1917 Fragments arranged by size: 2165 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 4633 5610 1203 Size: 3430 977 1327 Fragments arranged by size: 3430 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2589 1184 Size: 1405 4329 AlwNI CAG_nnn'CTG Cuts at: 3477 5049 3477 Size: 1572 4162 ApaI G_GGCC'C Cuts at: 2549 2549 Size: 5734 ApaLI G'TGCA_C Cuts at: 2047 2859 3791 4947 2047 Size: 812 932 1156 2834 Fragments arranged by size: 2834 1156 932 812 ApoI r'AATT_y Cuts at: 1493 2657 3043 3269 3421 5530 1493 Size: 1164 386 226 152 2109 1697 Fragments arranged by size: 2109 1697 1164 386 226 152 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2282 2561 3059 1870 Size: 73 339 279 498 4545 Fragments arranged by size: 4545 498 339 279 73 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3055 4510 618 Size: 325 217 627 1268 1455 1842 Fragments arranged by size: 1842 1455 1268 627 325 217 BanII G_rGCy'C Cuts at: 1391 2124 2283 2549 3053 1391 Size: 733 159 266 504 4072 Fragments arranged by size: 4072 733 504 266 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 2914 3009 928 Size: 971 317 698 95 3653 Fragments arranged by size: 3653 971 698 317 95 BbvCI CC'TCA_GC Cuts at: 2435 2435 Size: 5734 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2840 2874 2999 3033 3925 3959 1903 Size: 34 903 34 125 34 892 34 3678 Fragments arranged by size: 3678 903 892 125 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2497 3626 4836 2497 Size: 1129 1210 3395 Fragments arranged by size: 3395 1210 1129 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4345 136 Size: 107 122 71 631 605 2673 1525 Fragments arranged by size: 2673 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2457 5729 2457 Size: 3272 2462 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 2051 2549 2863 3795 4951 1790 Size: 261 498 314 932 1156 2573 Fragments arranged by size: 2573 1156 932 498 314 261 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5734 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2813 3465 4423 Size: 416 53 83 399 24 1396 652 958 Cuts at: 4423 442 Size: 1753 Fragments arranged by size: 1753 1396 958 652 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2007 4394 1239 Size: 768 2387 2579 Fragments arranged by size: 2579 2387 768 Bpu10I CC'TnA_GC Cuts at: 2435 2435 Size: 5734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2272 3859 4724 5022 2272 Size: 1587 865 298 2984 Fragments arranged by size: 2984 1587 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4397 882 Size: 617 2898 2219 Fragments arranged by size: 2898 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2573 3525 493 Size: 711 1369 952 2702 Fragments arranged by size: 2702 1369 952 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2919 3541 3923 4558 Size: 53 83 186 936 1386 622 382 635 Cuts at: 4558 275 Size: 1451 Fragments arranged by size: 1451 1386 936 635 622 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2170 4167 4565 4839 4986 5397 2170 Size: 1997 398 274 147 411 2507 Fragments arranged by size: 2507 1997 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5509 Fragments arranged by size: 5509 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 1186 Size: 1258 4476 BseYI C'CCAG_C Cuts at: 2586 4937 5433 2586 Size: 2351 496 2887 Fragments arranged by size: 2887 2351 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2140 2854 1163 Size: 573 404 714 4043 Fragments arranged by size: 4043 714 573 404 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2171 3184 3945 4973 1540 Size: 275 186 170 1013 761 1028 2301 Fragments arranged by size: 2301 1028 1013 761 275 186 170 BsiHKAI G_wGCw'C Cuts at: 2051 2863 3795 3880 4951 2051 Size: 812 932 85 1071 2834 Fragments arranged by size: 2834 1071 932 812 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2124 2283 2549 2863 3053 3795 Size: 399 261 73 159 266 314 190 742 Cuts at: 3795 3880 4951 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 314 266 261 190 159 85 73 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3089 844 Size: 2245 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3621 1394 Size: 429 1798 3507 Fragments arranged by size: 3507 1798 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2524 4229 4403 65 Size: 1183 1276 1705 174 1396 Fragments arranged by size: 1705 1396 1276 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4378 4565 1700 Size: 110 2568 187 2869 Fragments arranged by size: 2869 2568 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5734 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 2185 1184 Size: 1001 4733 BstYI r'GATC_y Cuts at: 1972 2421 2457 3064 3431 3816 3833 5285 5371 Size: 449 36 607 367 385 17 1452 86 Cuts at: 5371 5383 5729 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 607 449 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2328 513 Size: 552 939 324 3919 Fragments arranged by size: 3919 939 552 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2403 2778 487 Size: 683 56 1177 375 3443 Fragments arranged by size: 3443 1177 683 375 56 BtsI GCAGTG_nn' Cuts at: 2861 3250 4044 4064 2861 Size: 389 794 20 4531 Fragments arranged by size: 4531 794 389 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5699 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DraIII CAC_nnn'GTG Cuts at: 2832 2832 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3181 4070 5699 Size: 54 1425 50 275 18 1351 889 1629 Cuts at: 5699 8 Size: 43 Fragments arranged by size: 1629 1425 1351 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2256 2262 2530 3664 1353 Size: 903 6 268 1134 3423 Fragments arranged by size: 3423 1134 903 268 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2089 4321 4695 4841 387 Size: 1064 138 500 2232 374 146 1280 Fragments arranged by size: 2232 1280 1064 500 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 3797 4394 5181 5449 1239 Size: 393 375 1790 597 787 268 1524 Fragments arranged by size: 1790 1524 787 597 393 375 268 EcoO109I rG'GnC_Cy Cuts at: 2545 2546 2545 Size: 1 5733 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 2104 2561 4881 1678 Size: 426 457 2320 2531 Fragments arranged by size: 2531 2320 457 426 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2179 2211 4375 4407 4449 Size: 32 135 32 607 32 2164 32 42 Cuts at: 4449 4481 1373 Size: 32 2626 Fragments arranged by size: 2626 2164 607 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 3059 3059 Size: 5734 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3015 4848 5032 1305 Size: 1710 1833 184 2007 Fragments arranged by size: 2007 1833 1710 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3912 Fragments arranged by size: 3912 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2571 2785 3692 4051 4210 518 Size: 648 1405 214 907 359 159 2042 Fragments arranged by size: 2042 1405 907 648 359 214 159 MspA1I CmG'CkG Cuts at: 1606 2357 2652 3150 3827 4975 5220 1606 Size: 751 295 498 677 1148 245 2120 Fragments arranged by size: 2120 1148 751 677 498 295 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4243 Fragments arranged by size: 4243 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 3092 4637 1132 Size: 97 1863 1545 2229 Fragments arranged by size: 2229 1863 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5734 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5734 PfoI T'CCnGG_A Cuts at: 2023 2424 2528 2023 Size: 401 104 5229 Fragments arranged by size: 5229 401 104 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3835 5316 5348 3803 Size: 32 1481 32 4189 Fragments arranged by size: 4189 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2357 1613 Size: 744 4990 PsiI TTA'TAA Cuts at: 2304 3304 2304 Size: 1000 4734 PspOMI G'GGCC_C Cuts at: 2545 2545 Size: 5734 PstI C_TGCA'G Cuts at: 830 2868 3086 830 Size: 2038 218 3478 Fragments arranged by size: 3478 2038 218 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 PvuII CAG'CTG Cuts at: 2357 2652 2357 Size: 295 5439 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 2864 2970 3082 4217 4898 5089 826 Size: 219 1819 106 112 1135 681 191 1471 Fragments arranged by size: 1819 1471 1135 681 219 191 112 106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 3061 1872 Size: 1189 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2251 3838 4739 5001 820 Size: 197 926 308 1587 901 262 1553 Fragments arranged by size: 1587 1553 926 901 308 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5734 StyI C'CwwG_G Cuts at: 513 1065 2004 2499 3173 513 Size: 552 939 495 674 3074 Fragments arranged by size: 3074 939 674 552 495 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2679 3761 3778 3931 4116 4561 325 Size: 1255 1099 1082 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1099 1082 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3980 5692 96 Size: 274 80 33 51 1443 2003 1712 138 Fragments arranged by size: 2003 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2030 2051 2062 5694 5726 2019 Size: 11 21 11 3632 32 2027 Fragments arranged by size: 3632 2027 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2912 2912 Size: 5734 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 3059 1870 Size: 1189 4545 XmnI GAAnn'nnTTC Cuts at: 3863 3863 Size: 5734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI