(Circular) (Six-base) MAPSORT of: pFN21AB9658.seq Check: 915 from: 1 to: 5674 pFN21AB9658 5674 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2694 2821 2694 Size: 127 5547 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3017 1937 Size: 1080 4594 Acc65I G'GTAC_C Cuts at: 2995 2995 Size: 5674 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2206 2741 2762 2905 3737 5121 5389 1632 Size: 574 535 21 143 832 1384 268 1917 Fragments arranged by size: 1917 1384 832 574 535 268 143 21 AfeI AGC'GCT Cuts at: 2334 2334 Size: 5674 AflII C'TTAA_G Cuts at: 820 1017 2843 820 Size: 197 1826 3651 Fragments arranged by size: 3651 1826 197 AflIII A'CryG_T Cuts at: 1203 2433 2779 4573 5550 1203 Size: 1230 346 1794 977 1327 Fragments arranged by size: 1794 1327 1230 977 346 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2165 1184 Size: 981 4693 AlwNI CAG_nnn'CTG Cuts at: 2139 2365 3417 4989 2139 Size: 226 1052 1572 2824 Fragments arranged by size: 2824 1572 1052 226 ApaI G_GGCC'C Cuts at: 2474 2574 2474 Size: 100 5574 ApaLI G'TGCA_C Cuts at: 3731 4887 3731 Size: 1156 4518 ApoI r'AATT_y Cuts at: 1493 2983 3209 3361 5470 1493 Size: 1490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 226 152 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2249 2294 2315 2440 2567 2573 2960 Size: 73 306 45 21 125 127 6 387 Cuts at: 2960 2999 1870 Size: 39 4545 Fragments arranged by size: 4545 387 306 127 125 73 45 39 21 6 BamHI G'GATC_C Cuts at: 3004 3371 3004 Size: 367 5307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2444 2995 4450 618 Size: 325 217 627 657 551 1455 1842 Fragments arranged by size: 1842 1455 657 627 551 325 217 BanII G_rGCy'C Cuts at: 1391 2474 2574 2936 2993 1391 Size: 1083 100 362 57 4072 Fragments arranged by size: 4072 1083 362 100 57 BbeI G_GCGC'C Cuts at: 2448 2448 Size: 5674 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2246 2819 928 Size: 971 347 573 3783 Fragments arranged by size: 3783 971 573 347 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3865 3899 1903 Size: 34 1928 34 3678 Fragments arranged by size: 3678 1928 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2113 2453 4285 Size: 107 122 71 631 605 441 340 1832 Cuts at: 4285 136 Size: 1525 Fragments arranged by size: 1832 1525 631 605 441 340 122 107 71 BglII A'GATC_T Cuts at: 5669 5669 Size: 5674 BlpI GC'TnA_GC Cuts at: 2018 2183 3091 2018 Size: 165 908 4601 Fragments arranged by size: 4601 908 165 Bme1580I G_kGCm'C Cuts at: 1790 2208 2474 2574 3735 4891 1790 Size: 418 266 100 1161 1156 2573 Fragments arranged by size: 2573 1161 1156 418 266 100 BmgBI CAC'GTC Cuts at: 1318 2095 1318 Size: 777 4897 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2120 2345 2755 Size: 416 53 83 399 24 703 225 410 Cuts at: 2755 3405 4363 442 Size: 650 958 1753 Fragments arranged by size: 1753 958 703 650 416 410 399 225 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2538 1056 Size: 1482 4192 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2950 2950 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2822 4334 1239 Size: 1583 1512 2579 Fragments arranged by size: 2579 1583 1512 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2069 3799 4664 4962 2069 Size: 1730 865 298 2781 Fragments arranged by size: 2781 1730 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4337 882 Size: 617 2838 2219 Fragments arranged by size: 2838 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3465 493 Size: 711 2261 2702 Fragments arranged by size: 2702 2261 711 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2312 2445 2880 3481 Size: 53 83 186 936 779 133 435 601 Cuts at: 3481 3863 4498 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 779 635 601 435 382 186 133 83 53 BsaWI w'CCGG_w Cuts at: 4107 4505 4779 4926 5337 4107 Size: 398 274 147 411 4444 Fragments arranged by size: 4444 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2621 2651 2936 2966 1178 Size: 30 165 30 1218 30 285 30 3886 Fragments arranged by size: 3886 1218 285 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2944 1186 Size: 1758 3916 BseYI C'CCAG_C Cuts at: 2216 2467 4877 5373 2216 Size: 251 2410 496 2517 Fragments arranged by size: 2517 2410 496 251 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2125 1163 Size: 573 389 4712 Fragments arranged by size: 4712 573 389 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2232 3124 3885 4913 1540 Size: 275 186 231 892 761 1028 2301 Fragments arranged by size: 2301 1028 892 761 275 231 186 BsiHKAI G_wGCw'C Cuts at: 3735 3820 4891 3735 Size: 85 1071 4518 Fragments arranged by size: 4518 1071 85 BsmI GAATG_Cn' Cuts at: 3185 3278 3185 Size: 93 5581 BsmBI CGTCTCn'nnnn_ Cuts at: 2871 2871 Size: 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2474 2574 2936 2993 3735 3820 Size: 399 418 266 100 362 57 742 85 Cuts at: 3820 4891 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 418 399 362 266 100 85 57 BspEI T'CCGG_A Cuts at: 5337 5337 Size: 5674 BspHI T'CATG_A Cuts at: 3563 5293 3563 Size: 1730 3944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2088 2694 2760 2821 3029 844 Size: 1244 606 66 61 208 3489 Fragments arranged by size: 3489 1244 606 208 66 61 BsrBI CCG'CTC Cuts at: 1394 1823 2182 3561 1394 Size: 429 359 1379 3507 Fragments arranged by size: 3507 1379 429 359 BsrDI GCAATG_nn' Cuts at: 65 1248 4169 4343 65 Size: 1183 2921 174 1396 Fragments arranged by size: 2921 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2159 2447 4318 4505 1700 Size: 110 349 288 1871 187 2869 Fragments arranged by size: 2869 1871 349 288 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5674 BssHII G'CGCG_C Cuts at: 1925 2116 1925 Size: 191 5483 BssSI C'ACGA_G Cuts at: 2651 3734 4746 2651 Size: 1083 1012 3579 Fragments arranged by size: 3579 1083 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2139 2353 2551 794 Size: 1345 214 198 3917 Fragments arranged by size: 3917 1345 214 198 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstXI CCAn_nnnn'nTGG Cuts at: 2050 2050 Size: 5674 BstYI r'GATC_y Cuts at: 1972 2254 3004 3371 3756 3773 5225 5311 5323 Size: 282 750 367 385 17 1452 86 12 Cuts at: 5323 5669 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 750 385 367 346 282 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2055 513 Size: 552 939 6 45 4132 Fragments arranged by size: 4132 939 552 45 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4935 Fragments arranged by size: 4935 683 56 BtsI GCAGTG_nn' Cuts at: 3190 3984 4004 3190 Size: 794 20 4860 Fragments arranged by size: 4860 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DraIII CAC_nnn'GTG Cuts at: 2193 2353 2193 Size: 160 5514 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2052 3121 Size: 54 1425 50 275 18 177 45 1069 Cuts at: 3121 4010 5639 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1069 889 275 177 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3604 1353 Size: 2251 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2705 2749 4261 4635 4781 387 Size: 1064 138 1116 44 1512 374 146 1280 Fragments arranged by size: 1512 1280 1116 1064 374 146 138 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2741 2762 2822 2905 3737 4334 Size: 393 574 535 21 60 83 832 597 Cuts at: 4334 5121 5389 1239 Size: 787 268 1524 Fragments arranged by size: 1524 832 787 597 574 535 393 268 83 60 21 EcoO109I rG'GnC_Cy Cuts at: 2136 2136 Size: 5674 EcoRI G'AATT_C Cuts at: 2983 3361 2983 Size: 378 5296 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 2303 2336 2394 2448 2532 4821 1678 Size: 625 33 58 54 84 2289 2531 Fragments arranged by size: 2531 2289 625 84 58 54 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2621 2653 2936 2968 4315 Size: 32 135 32 1049 32 283 32 1347 Cuts at: 4315 4347 4389 4421 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1347 1049 283 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2068 3018 3264 669 Size: 988 281 130 950 246 3079 Fragments arranged by size: 3079 988 950 281 246 130 HindIII A'AGCT_T Cuts at: 748 2371 748 Size: 1623 4051 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KasI G'GCGC_C Cuts at: 2444 2444 Size: 5674 KpnI G_GTAC'C Cuts at: 2999 2999 Size: 5674 MfeI C'AATT_G Cuts at: 3273 3273 Size: 5674 MluI A'CGCG_T Cuts at: 2433 2779 5550 2433 Size: 346 2771 2557 Fragments arranged by size: 2771 2557 346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2664 4788 4972 1305 Size: 1359 2124 184 2007 Fragments arranged by size: 2124 2007 1359 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2054 10 Size: 54 1425 343 222 3630 Fragments arranged by size: 3630 1425 343 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 2663 3632 3991 4150 518 Size: 648 1497 969 359 159 2042 Fragments arranged by size: 2042 1497 969 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2050 2365 2551 3090 3767 4915 5160 Size: 406 38 315 186 539 677 1148 245 Cuts at: 5160 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 539 406 315 245 186 38 NaeI GCC'GGC Cuts at: 2161 2449 2161 Size: 288 5386 NarI GG'CG_CC Cuts at: 2445 2445 Size: 5674 NcoI C'CATG_G Cuts at: 513 1065 2004 2055 513 Size: 552 939 51 4132 Fragments arranged by size: 4132 939 552 51 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NgoMIV G'CCGG_C Cuts at: 2159 2447 2159 Size: 288 5386 NheI G'CTAG_C Cuts at: 1052 2534 1052 Size: 1482 4192 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 3032 4577 1132 Size: 97 1803 1545 2229 Fragments arranged by size: 2229 1803 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5674 PciI A'CATG_T Cuts at: 4573 4573 Size: 5674 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5674 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PspOMI G'GGCC_C Cuts at: 2470 2570 2470 Size: 100 5574 PspXI vC'TCGA_Gb Cuts at: 2249 2249 Size: 5674 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2738 2770 2738 Size: 32 5642 PstI C_TGCA'G Cuts at: 830 2154 3026 830 Size: 1324 872 3478 Fragments arranged by size: 3478 1324 872 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 PvuII CAG'CTG Cuts at: 2551 2551 Size: 5674 SacII CC_GC'GG Cuts at: 2013 2013 Size: 5674 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 3922 5634 3922 Size: 1712 3962 SfcI C'TryA_G Cuts at: 826 1045 2150 3022 4157 4838 5029 826 Size: 219 1105 872 1135 681 191 1471 Fragments arranged by size: 1471 1135 1105 872 681 219 191 SfoI GGC'GCC Cuts at: 2446 2446 Size: 5674 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 2447 1700 Size: 747 4927 SmaI CCC'GGG Cuts at: 1872 2442 2569 2575 3001 1872 Size: 570 127 6 426 4545 Fragments arranged by size: 4545 570 426 127 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 2249 2843 3778 4679 4941 Size: 197 926 141 165 594 935 901 262 Cuts at: 4941 820 Size: 1553 Fragments arranged by size: 1553 935 926 901 594 262 197 165 141 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SrfI GCCC'GGGC Cuts at: 2575 2575 Size: 5674 SspI AAT'ATT Cuts at: 5 52 3598 5 Size: 47 3546 2081 Fragments arranged by size: 3546 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 2004 2055 2700 3113 513 Size: 552 939 51 645 413 3074 Fragments arranged by size: 3074 939 645 552 413 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2246 2364 3701 3718 3871 4056 4501 Size: 1255 666 118 1337 17 153 185 445 Cuts at: 4501 325 Size: 1498 Fragments arranged by size: 1498 1337 1255 666 445 185 153 118 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 3920 5632 Size: 274 80 33 51 1443 282 1661 1712 Cuts at: 5632 96 Size: 138 Fragments arranged by size: 1712 1661 1443 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2047 2047 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 2249 1943 Size: 306 5368 XmaI C'CCGG_G Cuts at: 1870 2440 2567 2573 2999 1870 Size: 570 127 6 426 4545 Fragments arranged by size: 4545 570 426 127 6 XmnI GAAnn'nnTTC Cuts at: 3803 3803 Size: 5674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI RsrII SacI SanDI SapI SexAI SfiI SwaI