Restriction map for
pFN21AB9653
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BamHI BbrPI BbvCI
BciVI BclI BfaI BfrI BlnI
BpmI BsaAI BsaBI BsaI BsaWI
BseAI BsmAI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
EarI Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KpnI Ksp632I MaeI MaeIII
MamI MfeI MluNI MroI MscI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I Tsp509I TspRI Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 188 GTCTAC GT^[AC][GT]AC
AluI 791 AGCT AG^CT
AlwI 705 GATCC GGATC(4/5)
Aor51HI 119 AGCGCT AGC^GCT
Asp700I 36 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 16 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 442 GTCTTC GAAGAC(2/6)
BglI 296 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 542 AGATCT A^GATCT
BlpI 61 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 61 GCTCAGC GC^T[ACGT]AGC
BpuAI 442 GTCTTC GAAGAC(2/6)
BseRI 256 CTCCTC GAGGAG(10/8)
BsgI 349 CTGCAC GTGCAG(16/14)
BsiWI 569 CGTACG C^GTACG
BsrBI 751 CCGCTC CCGCTC(-3/-3)
BstF5I 762 CATCC GGATG(2/0)
BstXI 5 CCAAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 542 AGATCT [GA]^GATC[CT]
CelII 61 GCTCAGC GC^T[ACGT]AGC
Eco47III 119 AGCGCT AGC^GCT
EcoNI 258 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 762 CATCC GGATG(9/13)
MaeII 20 ACGT A^CGT
MflI 542 AGATCT [GA]^GATC[CT]
MslI 198 CATGCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 16 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 790 CAGCTG CAG^CTG
SfaNI 96 GATGC GCATC(5/9)
Tth111I 16 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 542 AGATCT [GA]^GATC[CT]
XmnI 36 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 228 GTAC GT^AC
570 GTAC
AflIII 438 ACGCGT A^C[GA][CT]GT
572 ACGCGT
AspHI 211 GAGCAC G[AT]GC[AT]^C
319 GAGCAC
AvaII 15 GGACC G^G[AT]CC
795 GGACC
BanII 59 GGGCTC G[GA]GC[CT]^C
525 GGGCTC
BcgI 318 CGAGCACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
538 GCAGAGATCTCG
BsiHKAI 211 GAGCAC G[AT]GC[AT]^C
319 GAGCAC
BsrFI 9 GCCGGT [GA]^CCGG[CT]
230 ACCGGC
Cfr10I 9 GCCGGT [GA]^CCGG[CT]
230 ACCGGC
DdeI 62 CTCAG C^T[ACGT]AG
380 CTGAG
HinfI 151 GAGTC G^A[ACGT]TC
382 GAGTC
MboII 443 TCTTC GAAGA(8/7)
446 TCTTC
MluI 438 ACGCGT A^CGCGT
572 ACGCGT
PleI 151 GAGTC GAGTC(4/5)
382 GAGTC
RsaI 228 GTAC GT^AC
570 GTAC
SmlI 259 CTCAAG C^T[CT][GA]AG
331 CTCAAG
TaqI 173 TCGA T^CGA
518 TCGA
TthHB8I 173 TCGA T^CGA
518 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AvaI BanI
BbeI BbvI BcnI BmyI BsaHI
BsaJI BsiEI BsiYI BslI BsoBI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DpnII DsaI EaeI
EagI EclXI Eco52I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
KasI KspI MboI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NotI
PstI SacII Sau3AI Sau96I ScrFI
SfcI SfoI SmaI TseI XmaCI
XmaI