(Circular) (Six-base) MAPSORT of: pFN21AB9653.seq Check: 2421 from: 1 to: 5533 pFN21AB9653 5533 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3343 278 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2194 2876 1937 Size: 257 682 4594 Fragments arranged by size: 4594 682 257 Acc65I G'GTAC_C Cuts at: 2854 2854 Size: 5533 AclI AA'CG_TT Cuts at: 1563 3660 4033 1563 Size: 2097 373 3063 Fragments arranged by size: 3063 2097 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3596 4980 5248 1632 Size: 1964 1384 268 1917 Fragments arranged by size: 1964 1917 1384 268 AfeI AGC'GCT Cuts at: 2126 2126 Size: 5533 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5336 AflIII A'CryG_T Cuts at: 1203 2443 2577 4432 5409 1203 Size: 1240 134 1855 977 1327 Fragments arranged by size: 1855 1327 1240 977 134 AgeI A'CCGG_T Cuts at: 4364 4364 Size: 5533 AhdI GACnn_n'nnGTC Cuts at: 4262 4262 Size: 5533 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5533 AlwNI CAG_nnn'CTG Cuts at: 3276 4848 3276 Size: 1572 3961 ApaLI G'TGCA_C Cuts at: 3590 4746 3590 Size: 1156 4377 ApoI r'AATT_y Cuts at: 1493 2842 3068 3220 5329 1493 Size: 1349 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 226 152 AseI AT'TA_AT Cuts at: 160 1032 4087 160 Size: 872 3055 1606 Fragments arranged by size: 3055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 AvaI C'yCGr_G Cuts at: 1870 1943 2405 2689 2698 2741 2858 1870 Size: 73 462 284 9 43 117 4545 Fragments arranged by size: 4545 462 284 117 73 43 9 BamHI G'GATC_C Cuts at: 2863 3230 2863 Size: 367 5166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 2290 2345 2380 2612 Size: 325 217 627 345 158 55 35 232 Cuts at: 2612 2854 4309 618 Size: 242 1455 1842 Fragments arranged by size: 1842 1455 627 345 325 242 232 217 158 55 35 BanII G_rGCy'C Cuts at: 1391 2068 2534 2852 1391 Size: 677 466 318 4072 Fragments arranged by size: 4072 677 466 318 BbeI G_GCGC'C Cuts at: 2136 2349 2616 2136 Size: 213 267 5053 Fragments arranged by size: 5053 267 213 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2440 928 Size: 971 541 4021 Fragments arranged by size: 4021 971 541 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2312 2346 2532 2566 3724 3758 1903 Size: 34 375 34 186 34 1158 34 3678 Fragments arranged by size: 3678 1158 375 186 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3425 4635 3425 Size: 1210 4323 BclI T'GATC_A Cuts at: 1548 1548 Size: 5533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2307 4144 136 Size: 107 122 71 631 605 635 1837 1525 Fragments arranged by size: 1837 1525 635 631 605 122 107 71 BglII A'GATC_T Cuts at: 2547 5528 2547 Size: 2981 2552 BlpI GC'TnA_GC Cuts at: 2067 2950 2067 Size: 883 4650 Bme1580I G_kGCm'C Cuts at: 1790 2295 2670 3594 4750 1790 Size: 505 375 924 1156 2573 Fragments arranged by size: 2573 1156 924 505 375 BmgBI CAC'GTC Cuts at: 1318 2026 1318 Size: 708 4825 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3264 4222 442 Size: 416 53 83 399 24 1847 958 1753 Fragments arranged by size: 1847 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5533 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4193 1239 Size: 2954 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2249 2321 3658 4523 4821 2249 Size: 72 1337 865 298 2961 Fragments arranged by size: 2961 1337 865 298 72 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4196 882 Size: 617 2697 2219 Fragments arranged by size: 2697 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3324 493 Size: 711 2120 2702 Fragments arranged by size: 2702 2120 711 BsaBI GATnn'nnATC Cuts at: 5527 5527 Size: 5533 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 2319 2346 2613 Size: 53 83 186 936 600 186 27 267 Cuts at: 2613 3340 3722 4357 275 Size: 727 382 635 1451 Fragments arranged by size: 1451 936 727 635 600 382 267 186 186 83 53 27 BsaWI w'CCGG_w Cuts at: 3966 4364 4638 4785 5196 3966 Size: 398 274 147 411 4303 Fragments arranged by size: 4303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2516 2546 1178 Size: 30 165 30 1113 30 4165 Fragments arranged by size: 4165 1113 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 1186 Size: 1066 4467 BseYI C'CCAG_C Cuts at: 2587 2684 2735 2793 4736 5232 2587 Size: 97 51 58 1943 496 2888 Fragments arranged by size: 2888 1943 496 97 58 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 1163 Size: 573 603 4357 Fragments arranged by size: 4357 603 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2171 2240 2418 2424 2466 2601 Size: 275 186 170 69 178 6 42 135 Cuts at: 2601 2652 2983 3744 4772 1540 Size: 51 331 761 1028 2301 Fragments arranged by size: 2301 1028 761 331 275 186 178 170 135 69 51 42 6 BsiHKAI G_wGCw'C Cuts at: 2220 2328 3594 3679 4750 2220 Size: 108 1266 85 1071 3003 Fragments arranged by size: 3003 1266 1071 108 85 BsiWI C'GTAC_G Cuts at: 2574 2574 Size: 5533 BsmI GAATG_Cn' Cuts at: 3044 3137 3044 Size: 93 5440 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2220 2295 2328 2534 2670 2852 Size: 399 278 152 75 33 206 136 182 Cuts at: 2852 3594 3679 4750 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 278 206 182 152 136 85 75 33 BspEI T'CCGG_A Cuts at: 5196 5196 Size: 5533 BspHI T'CATG_A Cuts at: 3422 5152 3422 Size: 1730 3803 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2888 844 Size: 2044 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2758 3420 1394 Size: 429 935 662 3507 Fragments arranged by size: 3507 935 662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4028 4202 65 Size: 1183 2780 174 1396 Fragments arranged by size: 2780 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2235 4177 4364 1700 Size: 110 204 221 1942 187 2869 Fragments arranged by size: 2869 1942 221 204 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5533 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5533 BssSI C'ACGA_G Cuts at: 3593 4605 3593 Size: 1012 4521 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5533 BstBI TT'CG_AA Cuts at: 4337 4337 Size: 5533 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5533 BstXI CCAn_nnnn'nTGG Cuts at: 2017 2017 Size: 5533 BstYI r'GATC_y Cuts at: 1972 2547 2863 3230 3615 3632 5084 5170 5182 Size: 575 316 367 385 17 1452 86 12 Cuts at: 5182 5528 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 575 385 367 346 316 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2240 2418 2499 2601 2620 2811 513 Size: 552 1175 178 81 102 19 191 3235 Fragments arranged by size: 3235 1175 552 191 178 102 81 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4794 Fragments arranged by size: 4794 683 56 BtsI GCAGTG_nn' Cuts at: 3049 3843 3863 3049 Size: 794 20 4719 Fragments arranged by size: 4719 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5498 DraI TTT'AAA Cuts at: 2837 2837 Size: 5533 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4540 809 Size: 507 3224 1802 Fragments arranged by size: 3224 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2168 2237 2415 Size: 54 1425 50 275 18 338 69 178 Cuts at: 2415 2421 2463 2649 2980 3869 5498 8 Size: 6 42 186 331 889 1629 43 Fragments arranged by size: 1629 1425 889 338 331 275 186 178 69 54 50 43 42 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2168 2237 2415 2421 2463 2649 2980 Size: 275 356 69 178 6 42 186 331 Cuts at: 2980 1537 Size: 4090 Fragments arranged by size: 4090 356 331 275 186 178 69 42 6 EarI CTCTTCn'nnn_ Cuts at: 1353 3463 1353 Size: 2110 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2330 4120 4494 4640 387 Size: 1064 138 741 1790 374 146 1280 Fragments arranged by size: 1790 1280 1064 741 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3596 4193 4980 5248 1239 Size: 393 1964 597 787 268 1524 Fragments arranged by size: 1964 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2267 2267 Size: 5533 EcoRI G'AATT_C Cuts at: 2842 3220 2842 Size: 378 5155 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2299 2299 Size: 5533 FspI TGC'GCA Cuts at: 4039 4039 Size: 5533 HaeII r_GCGC'y Cuts at: 1678 2128 2136 2349 2616 4680 1678 Size: 450 8 213 267 2064 2531 Fragments arranged by size: 2531 2064 450 267 213 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2516 2537 2548 2569 4174 Size: 32 135 32 944 21 11 21 1605 Cuts at: 4174 4206 4248 4280 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1605 944 135 42 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3123 669 Size: 988 281 939 246 3079 Fragments arranged by size: 3079 988 939 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5533 HpaI GTT'AAC Cuts at: 3123 3123 Size: 5533 KasI G'GCGC_C Cuts at: 2132 2345 2612 2132 Size: 213 267 5053 Fragments arranged by size: 5053 267 213 KpnI G_GTAC'C Cuts at: 2858 2858 Size: 5533 MfeI C'AATT_G Cuts at: 3132 3132 Size: 5533 MluI A'CGCG_T Cuts at: 2443 2577 5409 2443 Size: 134 2832 2567 Fragments arranged by size: 2832 2567 134 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 4647 4831 1305 Size: 834 2508 184 2007 Fragments arranged by size: 2508 2007 834 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3711 Fragments arranged by size: 3711 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 3491 3850 4009 518 Size: 648 1041 1284 359 159 2042 Fragments arranged by size: 2042 1284 1041 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2242 2420 2432 2468 2480 2501 2603 Size: 465 171 178 12 36 12 21 102 Cuts at: 2603 2645 2797 2813 2949 3626 4774 5019 1606 Size: 42 152 16 136 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 465 245 178 171 152 136 102 42 36 21 16 12 12 NarI GG'CG_CC Cuts at: 2133 2346 2613 2133 Size: 213 267 5053 Fragments arranged by size: 5053 267 213 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4981 NdeI CA'TA_TG Cuts at: 387 387 Size: 5533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5533 NotI GC'GGCC_GC Cuts at: 2415 2421 2649 2980 2415 Size: 6 228 331 4968 Fragments arranged by size: 4968 331 228 6 NspI r_CATG'y Cuts at: 1132 1229 2891 4436 1132 Size: 97 1662 1545 2229 Fragments arranged by size: 2229 1662 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5533 PciI A'CATG_T Cuts at: 4432 4432 Size: 5533 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5533 PmeI GTTT'AAAC Cuts at: 2837 2837 Size: 5533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3602 3634 5115 5147 3602 Size: 32 1481 32 3988 Fragments arranged by size: 3988 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5533 PsiI TTA'TAA Cuts at: 3103 3103 Size: 5533 PstI C_TGCA'G Cuts at: 830 2644 2726 2751 2885 830 Size: 1814 82 25 134 3478 Fragments arranged by size: 3478 1814 134 82 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5533 PvuII CAG'CTG Cuts at: 2797 2797 Size: 5533 SacII CC_GC'GG Cuts at: 2243 2421 2502 2604 2814 2243 Size: 178 81 102 210 4962 Fragments arranged by size: 4962 210 178 102 81 SalI G'TCGA_C Cuts at: 1936 2875 1936 Size: 939 4594 SbfI CC_TGCA'GG Cuts at: 2885 2885 Size: 5533 ScaI AGT'ACT Cuts at: 3781 5493 3781 Size: 1712 3821 SfcI C'TryA_G Cuts at: 826 1045 2640 2722 2747 2881 4016 4697 4888 Size: 219 1595 82 25 134 1135 681 191 Cuts at: 4888 826 Size: 1471 Fragments arranged by size: 1595 1471 1135 681 219 191 134 82 25 SfoI GGC'GCC Cuts at: 2134 2347 2614 2134 Size: 213 267 5053 Fragments arranged by size: 5053 267 213 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5533 SmaI CCC'GGG Cuts at: 1872 2691 2700 2743 2860 1872 Size: 819 9 43 117 4545 Fragments arranged by size: 4545 819 117 43 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 2264 2336 3637 4538 4800 820 Size: 197 926 321 72 1301 901 262 1553 Fragments arranged by size: 1553 1301 926 901 321 262 197 72 SnaBI TAC'GTA Cuts at: 493 3324 493 Size: 2831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5533 SphI G_CATG'C Cuts at: 1132 2891 1132 Size: 1759 3774 SrfI GCCC'GGGC Cuts at: 2691 2691 Size: 5533 SspI AAT'ATT Cuts at: 5 52 3457 5 Size: 47 3405 2081 Fragments arranged by size: 3405 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5533 StyI C'CwwG_G Cuts at: 513 1065 2972 513 Size: 552 1907 3074 Fragments arranged by size: 3074 1907 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3560 3577 3730 3915 4360 325 Size: 1255 1980 17 153 185 445 1498 Fragments arranged by size: 1980 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3779 5491 96 Size: 274 80 33 51 1443 1802 1712 138 Fragments arranged by size: 1802 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5493 5525 5493 Size: 32 5501 Tth111I GACn'n_nGTC Cuts at: 1316 2024 1316 Size: 708 4825 XbaI T'CTAG_A Cuts at: 2869 2869 Size: 5533 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5533 XmaI C'CCGG_G Cuts at: 1870 2689 2698 2741 2858 1870 Size: 819 9 43 117 4545 Fragments arranged by size: 4545 819 117 43 9 XmnI GAAnn'nnTTC Cuts at: 2045 3662 2045 Size: 1617 3916 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3341 276 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI