Restriction map for
pFN21AB9653
(Linear) (Six-base) MAPSORT of: KIBB9653.seq Check: 6488 from: 1 to: 828
KIBB9653.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 189 828
Size: 189 639
AfeI AGC'GCT
Cuts at: 0 121 828
Size: 121 707
AflIII A'CryG_T
Cuts at: 0 438 572 828
Size: 438 134 256
Fragments arranged by size:
438 256 134
AvaI C'yCGr_G
Cuts at: 0 400 684 693 736 828
Size: 400 284 9 43 92
Fragments arranged by size:
400 284 92 43 9
BanI G'GyrC_C
Cuts at: 0 127 285 340 375 607 828
Size: 127 158 55 35 232 221
Fragments arranged by size:
232 221 158 127 55 35
BanII G_rGCy'C
Cuts at: 0 63 529 828
Size: 63 466 299
Fragments arranged by size:
466 299 63
BbeI G_GCGC'C
Cuts at: 0 131 344 611 828
Size: 131 213 267 217
Fragments arranged by size:
267 217 213 131
BbsI GAAGACnn'nnnn_
Cuts at: 0 435 828
Size: 435 393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 307 341 527 561 828
Size: 307 34 186 34 267
Fragments arranged by size:
307 267 186 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 302 828
Size: 302 526
BglII A'GATC_T
Cuts at: 0 542 828
Size: 542 286
BlpI GC'TnA_GC
Cuts at: 0 62 828
Size: 62 766
Bme1580I G_kGCm'C
Cuts at: 0 290 665 828
Size: 290 375 163
Fragments arranged by size:
375 290 163
BmgBI CAC'GTC
Cuts at: 0 21 828
Size: 21 807
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 316 828
Size: 244 72 512
Fragments arranged by size:
512 244 72
BsaHI Gr'CG_yC
Cuts at: 0 128 314 341 608 828
Size: 128 186 27 267 220
Fragments arranged by size:
267 220 186 128 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 511 541 828
Size: 511 30 287
Fragments arranged by size:
511 287 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 828
Size: 247 581
BseYI C'CCAG_C
Cuts at: 0 582 679 730 788 828
Size: 582 97 51 58 40
Fragments arranged by size:
582 97 58 51 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 828
Size: 334 494
BsiEI CG_ry'CG
Cuts at: 0 166 235 413 419 461 596 647 828
Size: 166 69 178 6 42 135 51 181
Fragments arranged by size:
181 178 166 135 69 51 42 6
BsiHKAI G_wGCw'C
Cuts at: 0 215 323 828
Size: 215 108 505
Fragments arranged by size:
505 215 108
BsiWI C'GTAC_G
Cuts at: 0 569 828
Size: 569 259
Bsp1286I G_dGCh'C
Cuts at: 0 63 215 290 323 529 665 828
Size: 63 152 75 33 206 136 163
Fragments arranged by size:
206 163 152 136 75 63 33
BsrBI CCG'CTC
Cuts at: 0 753 828
Size: 753 75
BsrFI r'CCGG_y
Cuts at: 0 9 230 828
Size: 9 221 598
Fragments arranged by size:
598 221 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 828
Size: 12 816
BstYI r'GATC_y
Cuts at: 0 542 828
Size: 542 286
BtgI C'CryG_G
Cuts at: 0 235 413 494 596 615 806 828
Size: 235 178 81 102 19 191 22
Fragments arranged by size:
235 191 178 102 81 22 19
EaeI y'GGCC_r
Cuts at: 0 163 232 410 416 458 644 828
Size: 163 69 178 6 42 186 184
Fragments arranged by size:
186 184 178 163 69 42 6
EagI C'GGCC_G
Cuts at: 0 163 232 410 416 458 644 828
Size: 163 69 178 6 42 186 184
Fragments arranged by size:
186 184 178 163 69 42 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 325 828
Size: 325 503
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 828
Size: 262 566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 294 828
Size: 294 534
HaeII r_GCGC'y
Cuts at: 0 123 131 344 611 828
Size: 123 8 213 267 217
Fragments arranged by size:
267 217 213 123 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 532 543 564 828
Size: 511 21 11 21 264
Fragments arranged by size:
511 264 21 21 11
KasI G'GCGC_C
Cuts at: 0 127 340 607 828
Size: 127 213 267 221
Fragments arranged by size:
267 221 213 127
MluI A'CGCG_T
Cuts at: 0 438 572 828
Size: 438 134 256
Fragments arranged by size:
438 256 134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 828
Size: 134 694
MslI CAynn'nnrTG
Cuts at: 0 202 828
Size: 202 626
MspA1I CmG'CkG
Cuts at: 0 66 237 415 427 463 475 496 598
Size: 66 171 178 12 36 12 21 102
Cuts at: 598 640 792 808 828
Size: 42 152 16 20
Fragments arranged by size:
178 171 152 102 66 42 36 21
20 16 12 12
NarI GG'CG_CC
Cuts at: 0 128 341 608 828
Size: 128 213 267 220
Fragments arranged by size:
267 220 213 128
NotI GC'GGCC_GC
Cuts at: 0 410 416 644 828
Size: 410 6 228 184
Fragments arranged by size:
410 228 184 6
PstI C_TGCA'G
Cuts at: 0 639 721 746 828
Size: 639 82 25 82
Fragments arranged by size:
639 82 82 25
PvuII CAG'CTG
Cuts at: 0 792 828
Size: 792 36
SacII CC_GC'GG
Cuts at: 0 238 416 497 599 809 828
Size: 238 178 81 102 210 19
Fragments arranged by size:
238 210 178 102 81 19
SfcI C'TryA_G
Cuts at: 0 635 717 742 828
Size: 635 82 25 86
Fragments arranged by size:
635 86 82 25
SfoI GGC'GCC
Cuts at: 0 129 342 609 828
Size: 129 213 267 219
Fragments arranged by size:
267 219 213 129
SmaI CCC'GGG
Cuts at: 0 686 695 738 828
Size: 686 9 43 90
Fragments arranged by size:
686 90 43 9
SmlI C'TyrA_G
Cuts at: 0 259 331 828
Size: 259 72 497
Fragments arranged by size:
497 259 72
SrfI GCCC'GGGC
Cuts at: 0 686 828
Size: 686 142
Tth111I GACn'n_nGTC
Cuts at: 0 19 828
Size: 19 809
XmaI C'CCGG_G
Cuts at: 0 684 693 736 828
Size: 684 9 43 92
Fragments arranged by size:
684 92 43 9
XmnI GAAnn'nnTTC
Cuts at: 0 40 828
Size: 40 788
Enzymes that do cut:
AccI AfeI AflIII AvaI BanI BanII BbeI BbsI
BcgI BglI BglII BlpI Bme1580I BmgBI BpuEI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BsrBI BsrFI BstXI BstYI BtgI EaeI EagI EciI
EcoNI FalI HaeII Hin4I KasI MluI MmeI MslI
MspA1I NarI NotI PstI PvuII SacII SfcI SfoI
SmaI SmlI SrfI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MscI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI XbaI XcmI XhoI ZraI