(Circular) (Six-base) MAPSORT of: pFN21AB9649.seq Check: 2962 from: 1 to: 6028 pFN21AB9649 6028 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3838 278 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3371 1937 Size: 1434 4594 Acc65I G'GTAC_C Cuts at: 3349 3349 Size: 6028 AclI AA'CG_TT Cuts at: 1563 4155 4528 1563 Size: 2592 373 3063 Fragments arranged by size: 3063 2592 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 4091 5475 5743 1632 Size: 662 1797 1384 268 1917 Fragments arranged by size: 1917 1797 1384 662 268 AfeI AGC'GCT Cuts at: 2261 2261 Size: 6028 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5831 AflIII A'CryG_T Cuts at: 1203 2166 4927 5904 1203 Size: 963 2761 977 1327 Fragments arranged by size: 2761 1327 977 963 AgeI A'CCGG_T Cuts at: 4859 4859 Size: 6028 AhdI GACnn_n'nnGTC Cuts at: 4757 4757 Size: 6028 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6028 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6028 AlwNI CAG_nnn'CTG Cuts at: 2770 2803 3145 3771 5343 2770 Size: 33 342 626 1572 3455 Fragments arranged by size: 3455 1572 626 342 33 ApaLI G'TGCA_C Cuts at: 4085 5241 4085 Size: 1156 4872 ApoI r'AATT_y Cuts at: 1493 2078 3106 3337 3563 3715 5824 1493 Size: 585 1028 231 226 152 2109 1697 Fragments arranged by size: 2109 1697 1028 585 231 226 152 AseI AT'TA_AT Cuts at: 160 1032 4582 160 Size: 872 3550 1606 Fragments arranged by size: 3550 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 AvaI C'yCGr_G Cuts at: 1870 1943 2049 2626 2867 2911 3353 1870 Size: 73 106 577 241 44 442 4545 Fragments arranged by size: 4545 577 442 241 106 73 44 BamHI G'GATC_C Cuts at: 3358 3725 3358 Size: 367 5661 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2471 2534 2915 3349 4804 Size: 325 217 627 684 63 381 434 1455 Cuts at: 4804 618 Size: 1842 Fragments arranged by size: 1842 1455 684 627 434 381 325 217 63 BanII G_rGCy'C Cuts at: 1391 2417 2492 3230 3347 1391 Size: 1026 75 738 117 4072 Fragments arranged by size: 4072 1026 738 117 75 BbeI G_GCGC'C Cuts at: 2538 2919 2538 Size: 381 5647 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5057 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4219 4253 1903 Size: 34 2282 34 3678 Fragments arranged by size: 3678 2282 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3920 5130 3920 Size: 1210 4818 BclI T'GATC_A Cuts at: 1548 1548 Size: 6028 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2525 2782 4639 Size: 107 122 71 631 605 853 257 1857 Cuts at: 4639 136 Size: 1525 Fragments arranged by size: 1857 1525 853 631 605 257 122 107 71 BglII A'GATC_T Cuts at: 2238 6023 2238 Size: 3785 2243 BlpI GC'TnA_GC Cuts at: 2846 3266 3445 2846 Size: 420 179 5429 Fragments arranged by size: 5429 420 179 Bme1580I G_kGCm'C Cuts at: 1790 2832 4089 5245 1790 Size: 1042 1257 1156 2573 Fragments arranged by size: 2573 1257 1156 1042 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6028 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2557 3759 4717 Size: 416 53 83 399 24 1140 1202 958 Cuts at: 4717 442 Size: 1753 Fragments arranged by size: 1753 1202 1140 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6028 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2897 2897 Size: 6028 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2476 3314 4688 1239 Size: 1237 838 1374 2579 Fragments arranged by size: 2579 1374 1237 838 Bpu10I CC'TnA_GC Cuts at: 3098 3098 Size: 6028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2309 2498 4153 5018 5316 2309 Size: 189 1655 865 298 3021 Fragments arranged by size: 3021 1655 865 298 189 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4691 882 Size: 617 3192 2219 Fragments arranged by size: 3192 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2599 3819 493 Size: 711 1395 1220 2702 Fragments arranged by size: 2702 1395 1220 711 BsaBI GATnn'nnATC Cuts at: 6022 6022 Size: 6028 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2138 2535 2916 3075 Size: 53 83 186 936 605 397 381 159 Cuts at: 3075 3835 4217 4852 275 Size: 760 382 635 1451 Fragments arranged by size: 1451 936 760 635 605 397 382 381 186 159 83 53 BsaWI w'CCGG_w Cuts at: 2218 4461 4859 5133 5280 5691 2218 Size: 2243 398 274 147 411 2555 Fragments arranged by size: 2555 2243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5803 Fragments arranged by size: 5803 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 2753 3019 1186 Size: 898 669 266 4195 Fragments arranged by size: 4195 898 669 266 BseYI C'CCAG_C Cuts at: 2251 2399 2602 3145 5231 5727 2251 Size: 148 203 543 2086 496 2552 Fragments arranged by size: 2552 2086 543 496 203 148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2030 3238 1163 Size: 573 294 1208 3953 Fragments arranged by size: 3953 1208 573 294 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2159 2433 2529 3165 3478 4239 Size: 275 186 158 274 96 636 313 761 Cuts at: 4239 5267 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 636 313 275 274 186 158 96 BsiHKAI G_wGCw'C Cuts at: 4089 4174 5245 4089 Size: 85 1071 4872 Fragments arranged by size: 4872 1071 85 BsmI GAATG_Cn' Cuts at: 3539 3632 3539 Size: 93 5935 BsmBI CGTCTCn'nnnn_ Cuts at: 2800 2800 Size: 6028 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2417 2492 2832 3230 3347 4089 4174 Size: 399 627 75 340 398 117 742 85 Cuts at: 4174 5245 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 627 399 398 340 117 85 75 BspEI T'CCGG_A Cuts at: 2218 5691 2218 Size: 3473 2555 BspHI T'CATG_A Cuts at: 3917 5647 3917 Size: 1730 4298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 3383 844 Size: 1208 1331 3489 Fragments arranged by size: 3489 1331 1208 BsrBI CCG'CTC Cuts at: 1394 1823 2314 2622 2980 3915 1394 Size: 429 491 308 358 935 3507 Fragments arranged by size: 3507 935 491 429 358 308 BsrDI GCAATG_nn' Cuts at: 65 1248 4523 4697 65 Size: 1183 3275 174 1396 Fragments arranged by size: 3275 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2358 2432 2900 4672 4859 1700 Size: 110 548 74 468 1772 187 2869 Fragments arranged by size: 2869 1772 548 468 187 110 74 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6028 BssHII G'CGCG_C Cuts at: 1925 3120 1925 Size: 1195 4833 BssSI C'ACGA_G Cuts at: 4088 5100 4088 Size: 1012 5016 BstAPI GCAn_nnn'nTGC Cuts at: 794 2502 2770 794 Size: 1708 268 4052 Fragments arranged by size: 4052 1708 268 BstBI TT'CG_AA Cuts at: 4832 4832 Size: 6028 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6028 BstXI CCAn_nnnn'nTGG Cuts at: 2251 2251 Size: 6028 BstYI r'GATC_y Cuts at: 1972 2238 2289 3358 3725 4110 4127 5579 5665 Size: 266 51 1069 367 385 17 1452 86 Cuts at: 5665 5677 6023 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1069 385 367 346 266 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2523 2529 513 Size: 552 939 519 6 4012 Fragments arranged by size: 4012 939 552 519 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5289 Fragments arranged by size: 5289 683 56 BtsI GCAGTG_nn' Cuts at: 3544 4338 4358 3544 Size: 794 20 5214 Fragments arranged by size: 5214 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6028 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5993 DraI TTT'AAA Cuts at: 3332 3332 Size: 6028 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5035 809 Size: 507 3719 1802 Fragments arranged by size: 3719 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2156 2334 2391 Size: 54 1425 50 275 18 326 178 57 Cuts at: 2391 2430 2526 3162 3475 4364 5993 8 Size: 39 96 636 313 889 1629 43 Fragments arranged by size: 1629 1425 889 636 326 313 275 178 96 57 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2156 2430 2526 3162 3475 1537 Size: 275 344 274 96 636 313 4090 Fragments arranged by size: 4090 636 344 313 275 274 96 EarI CTCTTCn'nnn_ Cuts at: 1353 2417 3958 1353 Size: 1064 1541 3423 Fragments arranged by size: 3423 1541 1064 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 2324 2472 2873 4615 4989 Size: 1064 138 444 291 148 401 1742 374 Cuts at: 4989 5135 387 Size: 146 1280 Fragments arranged by size: 1742 1280 1064 444 401 374 291 148 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2476 3314 4091 4688 5475 5743 Size: 393 662 182 838 777 597 787 268 Cuts at: 5743 1239 Size: 1524 Fragments arranged by size: 1524 838 787 777 662 597 393 268 182 EcoO109I rG'GnC_Cy Cuts at: 2518 2518 Size: 6028 EcoRI G'AATT_C Cuts at: 2078 3337 3715 2078 Size: 1259 378 4391 Fragments arranged by size: 4391 1259 378 FspI TGC'GCA Cuts at: 4534 4534 Size: 6028 HaeII r_GCGC'y Cuts at: 1678 2060 2263 2406 2538 2919 3132 5175 1678 Size: 382 203 143 132 381 213 2043 2531 Fragments arranged by size: 2531 2043 382 381 213 203 143 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4669 4701 4743 4775 1373 Size: 32 135 32 3097 32 42 32 2626 Fragments arranged by size: 3097 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3372 3618 669 Size: 988 281 1434 246 3079 Fragments arranged by size: 3079 1434 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6028 HpaI GTT'AAC Cuts at: 3618 3618 Size: 6028 KasI G'GCGC_C Cuts at: 2534 2915 2534 Size: 381 5647 KpnI G_GTAC'C Cuts at: 3353 3353 Size: 6028 MfeI C'AATT_G Cuts at: 3627 3627 Size: 6028 MluI A'CGCG_T Cuts at: 2166 5904 2166 Size: 3738 2290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3333 5142 5326 1305 Size: 2028 1809 184 2007 Fragments arranged by size: 2028 2007 1809 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4206 Fragments arranged by size: 4206 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3986 4345 4504 518 Size: 648 2820 359 159 2042 Fragments arranged by size: 2820 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2525 2531 2713 2800 3045 3057 3066 3145 Size: 919 6 182 87 245 12 9 79 Cuts at: 3145 3444 4121 5269 5514 1606 Size: 299 677 1148 245 2120 Fragments arranged by size: 2120 1148 919 677 299 245 245 182 87 79 12 9 6 NaeI GCC'GGC Cuts at: 2434 2902 2434 Size: 468 5560 NarI GG'CG_CC Cuts at: 2535 2916 2535 Size: 381 5647 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4537 Fragments arranged by size: 4537 939 552 NdeI CA'TA_TG Cuts at: 387 3325 387 Size: 2938 3090 NgoMIV G'CCGG_C Cuts at: 2432 2900 2432 Size: 468 5560 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6028 NotI GC'GGCC_GC Cuts at: 2526 3475 2526 Size: 949 5079 NspI r_CATG'y Cuts at: 1132 1229 2227 3386 4931 1132 Size: 97 998 1159 1545 2229 Fragments arranged by size: 2229 1545 1159 998 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6028 PciI A'CATG_T Cuts at: 4927 4927 Size: 6028 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6028 PfoI T'CCnGG_A Cuts at: 2301 2301 Size: 6028 PmeI GTTT'AAAC Cuts at: 3332 3332 Size: 6028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4097 4129 5610 5642 4097 Size: 32 1481 32 4483 Fragments arranged by size: 4483 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6028 PsiI TTA'TAA Cuts at: 3598 3598 Size: 6028 PstI C_TGCA'G Cuts at: 830 2712 2718 2775 3380 830 Size: 1882 6 57 605 3478 Fragments arranged by size: 3478 1882 605 57 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6028 PvuII CAG'CTG Cuts at: 2713 2800 3145 2713 Size: 87 345 5596 Fragments arranged by size: 5596 345 87 SacII CC_GC'GG Cuts at: 2526 2532 2526 Size: 6 6022 SalI G'TCGA_C Cuts at: 1936 3370 1936 Size: 1434 4594 SapI GCTCTTCn'nnn_ Cuts at: 2417 2417 Size: 6028 SbfI CC_TGCA'GG Cuts at: 3380 3380 Size: 6028 ScaI AGT'ACT Cuts at: 4276 5988 4276 Size: 1712 4316 SexAI A'CCwGG_T Cuts at: 3312 3312 Size: 6028 SfcI C'TryA_G Cuts at: 826 1045 2708 2714 2771 2941 3376 4511 5192 Size: 219 1663 6 57 170 435 1135 681 Cuts at: 5192 5383 826 Size: 191 1471 Fragments arranged by size: 1663 1471 1135 681 435 219 191 170 57 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2525 2525 Size: 6028 SfoI GGC'GCC Cuts at: 2536 2917 2536 Size: 381 5647 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6028 SmaI CCC'GGG Cuts at: 1872 2051 3355 1872 Size: 179 1304 4545 Fragments arranged by size: 4545 1304 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 2324 2513 4132 5033 5295 820 Size: 197 926 381 189 1619 901 262 1553 Fragments arranged by size: 1619 1553 926 901 381 262 197 189 SnaBI TAC'GTA Cuts at: 493 3819 493 Size: 3326 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6028 SphI G_CATG'C Cuts at: 1132 3386 1132 Size: 2254 3774 SspI AAT'ATT Cuts at: 5 52 3952 5 Size: 47 3900 2081 Fragments arranged by size: 3900 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6028 StyI C'CwwG_G Cuts at: 513 1065 2004 2484 2538 3467 513 Size: 552 939 480 54 929 3074 Fragments arranged by size: 3074 939 929 552 480 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4055 4072 4225 4410 4855 325 Size: 1255 2475 17 153 185 445 1498 Fragments arranged by size: 2475 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4274 5986 96 Size: 274 80 33 51 1443 2297 1712 138 Fragments arranged by size: 2297 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5988 6020 5988 Size: 32 5996 Tth111I GACn'n_nGTC Cuts at: 1316 2137 1316 Size: 821 5207 XbaI T'CTAG_A Cuts at: 3364 3364 Size: 6028 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6028 XmaI C'CCGG_G Cuts at: 1870 2049 3353 1870 Size: 179 1304 4545 Fragments arranged by size: 4545 1304 179 XmnI GAAnn'nnTTC Cuts at: 2168 4157 2168 Size: 1989 4039 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3836 276 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SrfI SwaI XcmI