(Circular) (Six-base) MAPSORT of: pFN21AB9641.seq Check: 4662 from: 1 to: 5671 pFN21AB9641 5671 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3481 278 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 1937 Size: 1077 4594 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 3734 5118 5386 1632 Size: 650 1452 1384 268 1917 Fragments arranged by size: 1917 1452 1384 650 268 AfeI AGC'GCT Cuts at: 2219 2693 2219 Size: 474 5197 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 4570 5547 1203 Size: 3367 977 1327 Fragments arranged by size: 3367 1327 977 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5671 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2055 1184 Size: 871 4800 AlwNI CAG_nnn'CTG Cuts at: 2185 3414 4986 2185 Size: 1229 1572 2870 Fragments arranged by size: 2870 1572 1229 ApaI G_GGCC'C Cuts at: 2155 2381 2155 Size: 226 5445 ApaLI G'TGCA_C Cuts at: 3728 4884 3728 Size: 1156 4515 ApoI r'AATT_y Cuts at: 1493 2980 3206 3358 5467 1493 Size: 1487 226 152 2109 1697 Fragments arranged by size: 2109 1697 1487 226 152 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2080 2660 2996 1870 Size: 73 137 580 336 4545 Fragments arranged by size: 4545 580 336 137 73 BamHI G'GATC_C Cuts at: 3001 3368 3001 Size: 367 5304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 2666 2992 4447 618 Size: 325 217 627 283 596 326 1455 1842 Fragments arranged by size: 1842 1455 627 596 326 325 283 217 BanII G_rGCy'C Cuts at: 1391 2087 2155 2381 2549 2589 2714 2990 1391 Size: 696 68 226 168 40 125 276 4072 Fragments arranged by size: 4072 696 276 226 168 125 68 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4700 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3862 3896 1903 Size: 34 1925 34 3678 Fragments arranged by size: 3678 1925 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 1548 Size: 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4282 136 Size: 107 122 71 631 605 2610 1525 Fragments arranged by size: 2610 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 3088 3088 Size: 5671 Bme1580I G_kGCm'C Cuts at: 1790 2073 2155 2381 2669 2784 3732 4888 1790 Size: 283 82 226 288 115 948 1156 2573 Fragments arranged by size: 2573 1156 948 288 283 226 115 82 BmgBI CAC'GTC Cuts at: 1318 2233 1318 Size: 915 4756 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3402 4360 442 Size: 416 53 83 399 24 1985 958 1753 Fragments arranged by size: 1985 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2206 2686 2206 Size: 480 5191 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4331 1239 Size: 3092 2579 Bpu10I CC'TnA_GC Cuts at: 2086 2086 Size: 5671 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2465 3796 4661 4959 2465 Size: 1331 865 298 3177 Fragments arranged by size: 3177 1331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2912 4334 882 Size: 617 1413 1422 2219 Fragments arranged by size: 2219 1422 1413 617 BsaAI yAC'GTr Cuts at: 493 1204 3462 493 Size: 711 2258 2702 Fragments arranged by size: 2702 2258 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3478 3860 4495 275 Size: 53 83 186 936 1945 382 635 1451 Fragments arranged by size: 1945 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 4502 4776 4923 5334 4104 Size: 398 274 147 411 4441 Fragments arranged by size: 4441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5446 Fragments arranged by size: 5446 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2654 2657 2846 2930 1186 Size: 1468 3 189 84 3927 Fragments arranged by size: 3927 1468 189 84 3 BseYI C'CCAG_C Cuts at: 2047 2374 2381 4874 5370 2047 Size: 327 7 2493 496 2348 Fragments arranged by size: 2493 2348 496 327 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5098 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2500 2652 3121 3882 4910 1540 Size: 275 186 499 152 469 761 1028 2301 Fragments arranged by size: 2301 1028 761 499 469 275 186 152 BsiHKAI G_wGCw'C Cuts at: 2364 3732 3817 4888 2364 Size: 1368 85 1071 3147 Fragments arranged by size: 3147 1368 1071 85 BsmI GAATG_Cn' Cuts at: 3182 3275 3182 Size: 93 5578 BsmBI CGTCTCn'nnnn_ Cuts at: 2168 2168 Size: 5671 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2087 2155 2364 2381 2549 2589 Size: 399 283 14 68 209 17 168 40 Cuts at: 2589 2669 2714 2784 2990 3732 3817 4888 1391 Size: 80 45 70 206 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 283 209 206 168 85 80 70 68 45 40 17 14 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 3560 5290 3560 Size: 1730 3941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3026 844 Size: 2182 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3558 1394 Size: 429 1735 3507 Fragments arranged by size: 3507 1735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2565 4166 4340 65 Size: 1183 1317 1601 174 1396 Fragments arranged by size: 1601 1396 1317 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2647 4315 4502 1700 Size: 110 837 1668 187 2869 Fragments arranged by size: 2869 1668 837 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5671 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 2171 3731 4743 2171 Size: 1560 1012 3099 Fragments arranged by size: 3099 1560 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5671 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5671 BstXI CCAn_nnnn'nTGG Cuts at: 2200 2320 2200 Size: 120 5551 BstYI r'GATC_y Cuts at: 1972 2476 3001 3368 3753 3770 5222 5308 5320 Size: 504 525 367 385 17 1452 86 12 Cuts at: 5320 5666 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 525 504 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2325 513 Size: 552 939 321 3859 Fragments arranged by size: 3859 939 552 321 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 2137 3187 3981 4001 2137 Size: 1050 794 20 3807 Fragments arranged by size: 3807 1050 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5636 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2497 2610 Size: 54 1425 50 275 18 492 175 113 Cuts at: 2610 2649 3118 4007 5636 8 Size: 39 469 889 1629 43 Fragments arranged by size: 1629 1425 889 492 469 275 175 113 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2497 2649 3118 1537 Size: 275 685 152 469 4090 Fragments arranged by size: 4090 685 469 275 152 EarI CTCTTCn'nnn_ Cuts at: 1353 3601 1353 Size: 2248 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2504 4258 4632 4778 387 Size: 1064 138 435 480 1754 374 146 1280 Fragments arranged by size: 1754 1280 1064 480 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 3734 4331 5118 5386 1239 Size: 393 650 1452 597 787 268 1524 Fragments arranged by size: 1524 1452 787 650 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2402 2402 Size: 5671 EcoO109I rG'GnC_Cy Cuts at: 2151 2152 2378 2534 2151 Size: 1 226 156 5288 Fragments arranged by size: 5288 226 156 1 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2272 2713 2272 Size: 441 5230 FspI TGC'GCA Cuts at: 2510 4177 2510 Size: 1667 4004 HaeII r_GCGC'y Cuts at: 1678 2048 2221 2432 2695 4818 1678 Size: 370 173 211 263 2123 2531 Fragments arranged by size: 2531 2123 370 263 211 173 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2696 2728 4312 4344 4386 Size: 32 135 32 1124 32 1584 32 42 Cuts at: 4386 4418 1373 Size: 32 2626 Fragments arranged by size: 2626 1584 1124 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2527 3015 3261 669 Size: 988 281 589 488 246 3079 Fragments arranged by size: 3079 988 589 488 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 3270 3270 Size: 5671 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2739 4785 4969 1305 Size: 1434 2046 184 2007 Fragments arranged by size: 2046 2007 1434 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2612 10 Size: 54 1425 343 780 3069 Fragments arranged by size: 3069 1425 780 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 2777 3629 3988 4147 518 Size: 648 1152 459 852 359 159 2042 Fragments arranged by size: 2042 1152 852 648 459 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2374 2927 3087 3764 4912 5157 1606 Size: 416 352 553 160 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 553 416 352 245 160 NaeI GCC'GGC Cuts at: 2649 2649 Size: 5671 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4180 Fragments arranged by size: 4180 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NgoMIV G'CCGG_C Cuts at: 2647 2647 Size: 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 2497 3118 2497 Size: 621 5050 NspI r_CATG'y Cuts at: 1132 1229 3029 4574 1132 Size: 97 1800 1545 2229 Fragments arranged by size: 2229 1800 1545 97 PasI CC'CwG_GG Cuts at: 1348 2065 2156 1348 Size: 717 91 4863 Fragments arranged by size: 4863 717 91 PciI A'CATG_T Cuts at: 4570 4570 Size: 5671 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5671 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3740 3772 5253 5285 3740 Size: 32 1481 32 4126 Fragments arranged by size: 4126 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5671 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PspOMI G'GGCC_C Cuts at: 2151 2377 2151 Size: 226 5445 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2450 2482 2450 Size: 32 5639 PstI C_TGCA'G Cuts at: 830 2169 2946 3023 830 Size: 1339 777 77 3478 Fragments arranged by size: 3478 1339 777 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 PvuII CAG'CTG Cuts at: 2374 2374 Size: 5671 RsrII CG'GwC_CG Cuts at: 2011 2011 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 2468 2825 3919 5631 2468 Size: 357 1094 1712 2508 Fragments arranged by size: 2508 1712 1094 357 SfcI C'TryA_G Cuts at: 826 1045 2165 2942 3019 4154 4835 5026 826 Size: 219 1120 777 77 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 777 681 219 191 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2998 1872 Size: 1126 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2480 3775 4676 4938 820 Size: 197 926 537 1295 901 262 1553 Fragments arranged by size: 1553 1295 926 901 537 262 197 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5671 StyI C'CwwG_G Cuts at: 513 1065 2004 2247 3110 513 Size: 552 939 243 863 3074 Fragments arranged by size: 3074 939 863 552 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2028 3698 3715 3868 4053 4498 325 Size: 1255 448 1670 17 153 185 445 1498 Fragments arranged by size: 1670 1498 1255 448 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2466 2823 3917 Size: 274 80 33 51 1443 489 357 1094 Cuts at: 3917 5629 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1094 489 357 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5631 5663 5631 Size: 32 5639 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5671 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5671 XmaI C'CCGG_G Cuts at: 1870 2996 1870 Size: 1126 4545 XmnI GAAnn'nnTTC Cuts at: 3800 3800 Size: 5671 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3479 276 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI