(Circular) (Six-base) MAPSORT of: pFN21AB9636.seq Check: 8243 from: 1 to: 5521 pFN21AB9636 5521 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3331 278 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2864 1937 Size: 927 4594 Acc65I G'GTAC_C Cuts at: 2842 2842 Size: 5521 AclI AA'CG_TT Cuts at: 1563 3648 4021 1563 Size: 2085 373 3063 Fragments arranged by size: 3063 2085 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 3584 4968 5236 1632 Size: 650 1302 1384 268 1917 Fragments arranged by size: 1917 1384 1302 650 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5324 AflIII A'CryG_T Cuts at: 1203 4420 5397 1203 Size: 3217 977 1327 Fragments arranged by size: 3217 1327 977 AgeI A'CCGG_T Cuts at: 4352 4352 Size: 5521 AhdI GACnn_n'nnGTC Cuts at: 4250 4250 Size: 5521 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2306 1184 Size: 1122 4399 AlwNI CAG_nnn'CTG Cuts at: 3264 4836 3264 Size: 1572 3949 ApaLI G'TGCA_C Cuts at: 2537 2546 3578 4734 2537 Size: 9 1032 1156 3324 Fragments arranged by size: 3324 1156 1032 9 ApoI r'AATT_y Cuts at: 1493 2008 2830 3056 3208 5317 1493 Size: 515 822 226 152 2109 1697 Fragments arranged by size: 2109 1697 822 515 226 152 AseI AT'TA_AT Cuts at: 160 1032 4075 160 Size: 872 3043 1606 Fragments arranged by size: 3043 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 AvaI C'yCGr_G Cuts at: 1870 1943 2159 2846 1870 Size: 73 216 687 4545 Fragments arranged by size: 4545 687 216 73 BamHI G'GATC_C Cuts at: 2851 3218 2851 Size: 367 5154 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 2624 2656 2842 4297 Size: 325 217 627 396 441 32 186 1455 Cuts at: 4297 618 Size: 1842 Fragments arranged by size: 1842 1455 627 441 396 325 217 186 32 BanII G_rGCy'C Cuts at: 1391 2024 2840 1391 Size: 633 816 4072 Fragments arranged by size: 4072 816 633 BbeI G_GCGC'C Cuts at: 2187 2660 2187 Size: 473 5048 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4550 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3712 3746 1903 Size: 34 1775 34 3678 Fragments arranged by size: 3678 1775 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3413 4623 3413 Size: 1210 4311 BclI T'GATC_A Cuts at: 1548 1548 Size: 5521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2783 4132 136 Size: 107 122 71 631 605 1111 1349 1525 Fragments arranged by size: 1525 1349 1111 631 605 122 107 71 BglII A'GATC_T Cuts at: 2454 5516 2454 Size: 3062 2459 BlpI GC'TnA_GC Cuts at: 2938 2938 Size: 5521 Bme1580I G_kGCm'C Cuts at: 1790 2541 2550 3582 4738 1790 Size: 751 9 1032 1156 2573 Fragments arranged by size: 2573 1156 1032 751 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5521 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2713 3252 4210 Size: 416 53 83 399 24 1296 539 958 Cuts at: 4210 442 Size: 1753 Fragments arranged by size: 1753 1296 958 539 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5521 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2014 2447 4181 1239 Size: 775 433 1734 2579 Fragments arranged by size: 2579 1734 775 433 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2636 2801 3646 4511 4809 2636 Size: 165 845 865 298 3348 Fragments arranged by size: 3348 865 845 298 165 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2128 4184 882 Size: 617 629 2056 2219 Fragments arranged by size: 2219 2056 629 617 BsaAI yAC'GTr Cuts at: 493 1204 3312 493 Size: 711 2108 2702 Fragments arranged by size: 2702 2108 711 BsaBI GATnn'nnATC Cuts at: 5515 5515 Size: 5521 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2184 2657 3328 3710 Size: 53 83 186 936 651 473 671 382 Cuts at: 3710 4345 275 Size: 635 1451 Fragments arranged by size: 1451 936 671 651 635 473 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2047 3954 4352 4626 4773 5184 2047 Size: 1907 398 274 147 411 2384 Fragments arranged by size: 2384 1907 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5296 Fragments arranged by size: 5296 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 1186 Size: 1258 4263 BseYI C'CCAG_C Cuts at: 2683 4724 5220 2683 Size: 2041 496 2984 Fragments arranged by size: 2984 2041 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2710 1163 Size: 573 974 3974 Fragments arranged by size: 3974 974 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2508 2799 2971 3732 4760 1540 Size: 275 186 507 291 172 761 1028 2301 Fragments arranged by size: 2301 1028 761 507 291 275 186 172 BsiHKAI G_wGCw'C Cuts at: 2024 2541 2550 3582 3667 4738 2024 Size: 517 9 1032 85 1071 2807 Fragments arranged by size: 2807 1071 1032 517 85 9 BsmI GAATG_Cn' Cuts at: 3032 3125 3032 Size: 93 5428 BsmBI CGTCTCn'nnnn_ Cuts at: 2117 2117 Size: 5521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2541 2550 2840 3582 3667 4738 Size: 399 234 517 9 290 742 85 1071 Cuts at: 4738 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 517 399 290 234 85 9 BspEI T'CCGG_A Cuts at: 2047 5184 2047 Size: 3137 2384 BspHI T'CATG_A Cuts at: 3410 5140 3410 Size: 1730 3791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2876 844 Size: 2032 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2765 3408 1394 Size: 429 942 643 3507 Fragments arranged by size: 3507 942 643 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4016 4190 65 Size: 1183 2768 174 1396 Fragments arranged by size: 2768 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2507 4165 4352 1700 Size: 110 697 1658 187 2869 Fragments arranged by size: 2869 1658 697 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5521 BssHII G'CGCG_C Cuts at: 1925 2565 1925 Size: 640 4881 BssSI C'ACGA_G Cuts at: 3581 4593 3581 Size: 1012 4509 BstAPI GCAn_nnn'nTGC Cuts at: 794 2155 2545 2733 794 Size: 1361 390 188 3582 Fragments arranged by size: 3582 1361 390 188 BstBI TT'CG_AA Cuts at: 4325 4325 Size: 5521 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5521 BstXI CCAn_nnnn'nTGG Cuts at: 2425 2425 Size: 5521 BstYI r'GATC_y Cuts at: 1972 2454 2851 3218 3603 3620 5072 5158 5170 Size: 482 397 367 385 17 1452 86 12 Cuts at: 5170 5516 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 482 397 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2069 2069 Size: 5521 BtgI C'CryG_G Cuts at: 513 1065 2193 2310 2493 513 Size: 552 1128 117 183 3541 Fragments arranged by size: 3541 1128 552 183 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4782 Fragments arranged by size: 4782 683 56 BtsI GCAGTG_nn' Cuts at: 2482 3037 3831 3851 2482 Size: 555 794 20 4152 Fragments arranged by size: 4152 794 555 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5486 DraI TTT'AAA Cuts at: 2825 2825 Size: 5521 DraIII CAC_nnn'GTG Cuts at: 2545 2545 Size: 5521 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4528 809 Size: 507 3212 1802 Fragments arranged by size: 3212 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2505 2509 2526 Size: 54 1425 50 275 18 675 4 17 Cuts at: 2526 2796 2968 3857 5486 8 Size: 270 172 889 1629 43 Fragments arranged by size: 1629 1425 889 675 275 270 172 54 50 43 18 17 4 EagI C'GGCC_G Cuts at: 1537 1812 2505 2796 2968 1537 Size: 275 693 291 172 4090 Fragments arranged by size: 4090 693 291 275 172 EarI CTCTTCn'nnn_ Cuts at: 1353 2432 3451 1353 Size: 1079 1019 3423 Fragments arranged by size: 3423 1079 1019 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2590 4108 4482 4628 387 Size: 1064 138 1001 1518 374 146 1280 Fragments arranged by size: 1518 1280 1064 1001 374 146 138 EcoICRI GAG'CTC Cuts at: 2022 2022 Size: 5521 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2014 2282 2447 3584 4181 4968 5236 Size: 393 382 268 165 1137 597 787 268 Cuts at: 5236 1239 Size: 1524 Fragments arranged by size: 1524 1137 787 597 393 382 268 268 165 EcoO109I rG'GnC_Cy Cuts at: 2242 2242 Size: 5521 EcoRI G'AATT_C Cuts at: 2008 2830 3208 2008 Size: 822 378 4321 Fragments arranged by size: 4321 822 378 FseI GG_CCGG'CC Cuts at: 2511 2511 Size: 5521 FspI TGC'GCA Cuts at: 2360 4027 2360 Size: 1667 3854 HaeII r_GCGC'y Cuts at: 1678 2187 2660 4668 1678 Size: 509 473 2008 2531 Fragments arranged by size: 2531 2008 509 473 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 4162 4194 4236 Size: 32 135 32 434 32 2124 32 42 Cuts at: 4236 4268 1373 Size: 32 2626 Fragments arranged by size: 2626 2124 434 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2865 3111 669 Size: 988 281 927 246 3079 Fragments arranged by size: 3079 988 927 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5521 HpaI GTT'AAC Cuts at: 3111 3111 Size: 5521 KasI G'GCGC_C Cuts at: 2183 2656 2183 Size: 473 5048 KpnI G_GTAC'C Cuts at: 2846 2846 Size: 5521 MfeI C'AATT_G Cuts at: 3120 3120 Size: 5521 MluI A'CGCG_T Cuts at: 5397 5397 Size: 5521 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2214 4635 4819 1305 Size: 909 2421 184 2007 Fragments arranged by size: 2421 2007 909 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3699 Fragments arranged by size: 3699 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 3479 3838 3997 518 Size: 648 1242 1071 359 159 2042 Fragments arranged by size: 2042 1242 1071 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2195 2230 2596 2683 2691 2937 3614 4762 Size: 589 35 366 87 8 246 677 1148 Cuts at: 4762 5007 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 589 366 246 245 87 35 8 NaeI GCC'GGC Cuts at: 2509 2509 Size: 5521 NarI GG'CG_CC Cuts at: 2184 2657 2184 Size: 473 5048 NcoI C'CATG_G Cuts at: 513 1065 2310 513 Size: 552 1245 3724 Fragments arranged by size: 3724 1245 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5521 NgoMIV G'CCGG_C Cuts at: 2507 2507 Size: 5521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5521 NotI GC'GGCC_GC Cuts at: 2968 2968 Size: 5521 NspI r_CATG'y Cuts at: 1132 1229 2879 4424 1132 Size: 97 1650 1545 2229 Fragments arranged by size: 2229 1650 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5521 PciI A'CATG_T Cuts at: 4420 4420 Size: 5521 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5521 PmeI GTTT'AAAC Cuts at: 2825 2825 Size: 5521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3590 3622 5103 5135 3590 Size: 32 1481 32 3976 Fragments arranged by size: 3976 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 2242 Size: 5521 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5521 PsiI TTA'TAA Cuts at: 3091 3091 Size: 5521 PstI C_TGCA'G Cuts at: 830 2682 2873 830 Size: 1852 191 3478 Fragments arranged by size: 3478 1852 191 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5521 PvuII CAG'CTG Cuts at: 2230 2683 2230 Size: 453 5068 SacI G_AGCT'C Cuts at: 2024 2024 Size: 5521 SacII CC_GC'GG Cuts at: 2196 2196 Size: 5521 SalI G'TCGA_C Cuts at: 1936 2863 1936 Size: 927 4594 SbfI CC_TGCA'GG Cuts at: 2873 2873 Size: 5521 ScaI AGT'ACT Cuts at: 3769 5481 3769 Size: 1712 3809 SfcI C'TryA_G Cuts at: 826 1045 2246 2678 2869 4004 4685 4876 826 Size: 219 1201 432 191 1135 681 191 1471 Fragments arranged by size: 1471 1201 1135 681 432 219 191 191 SfoI GGC'GCC Cuts at: 2185 2658 2185 Size: 473 5048 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5521 SmaI CCC'GGG Cuts at: 1872 2161 2848 1872 Size: 289 687 4545 Fragments arranged by size: 4545 687 289 SmlI C'TyrA_G Cuts at: 820 1017 1943 2651 2816 3625 4526 4788 820 Size: 197 926 708 165 809 901 262 1553 Fragments arranged by size: 1553 926 901 809 708 262 197 165 SnaBI TAC'GTA Cuts at: 493 3312 493 Size: 2819 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5521 SphI G_CATG'C Cuts at: 1132 2879 1132 Size: 1747 3774 SspI AAT'ATT Cuts at: 5 52 3445 5 Size: 47 3393 2081 Fragments arranged by size: 3393 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5521 StyI C'CwwG_G Cuts at: 513 1065 2187 2310 2805 2960 513 Size: 552 1122 123 495 155 3074 Fragments arranged by size: 3074 1122 552 495 155 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2110 3548 3565 3718 3903 4348 325 Size: 1255 530 1438 17 153 185 445 1498 Fragments arranged by size: 1498 1438 1255 530 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 3767 5479 Size: 274 80 33 51 1443 234 1556 1712 Cuts at: 5479 96 Size: 138 Fragments arranged by size: 1712 1556 1443 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5481 5513 5481 Size: 32 5489 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5521 XbaI T'CTAG_A Cuts at: 2857 2857 Size: 5521 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2705 2779 2705 Size: 74 5447 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5521 XmaI C'CCGG_G Cuts at: 1870 2159 2846 1870 Size: 289 687 4545 Fragments arranged by size: 4545 687 289 XmnI GAAnn'nnTTC Cuts at: 3650 3650 Size: 5521 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3329 276 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI