(Circular) (Six-base) MAPSORT of: pFN21AB9633.seq Check: 836 from: 1 to: 6091 pFN21AB9633 6091 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2287 3434 1937 Size: 350 1147 4594 Fragments arranged by size: 4594 1147 350 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3363 4154 5538 5806 1632 Size: 1731 791 1384 268 1917 Fragments arranged by size: 1917 1731 1384 791 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2946 1184 Size: 1762 4329 AlwNI CAG_nnn'CTG Cuts at: 3386 3834 5406 3386 Size: 448 1572 4071 Fragments arranged by size: 4071 1572 448 ApaI G_GGCC'C Cuts at: 2897 2897 Size: 6091 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2198 2752 2959 3035 3400 3626 3778 5887 Size: 705 554 207 76 365 226 152 2109 Cuts at: 5887 1493 Size: 1697 Fragments arranged by size: 2109 1697 705 554 365 226 207 152 76 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2818 3416 1870 Size: 73 875 598 4545 Fragments arranged by size: 4545 875 598 73 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3412 4867 618 Size: 325 217 627 1625 1455 1842 Fragments arranged by size: 1842 1625 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2612 2897 3410 1391 Size: 1221 285 513 4072 Fragments arranged by size: 4072 1221 513 285 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2121 2877 928 Size: 971 222 756 4142 Fragments arranged by size: 4142 971 756 222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4282 4316 1903 Size: 34 50 34 2261 34 3678 Fragments arranged by size: 3678 2261 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2270 6086 2270 Size: 3816 2275 BlpI GC'TnA_GC Cuts at: 2355 3508 2355 Size: 1153 4938 Bme1580I G_kGCm'C Cuts at: 1790 2897 3115 4152 5308 1790 Size: 1107 218 1037 1156 2573 Fragments arranged by size: 2573 1156 1107 1037 218 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2336 3822 4780 Size: 416 53 83 399 24 919 1486 958 Cuts at: 4780 442 Size: 1753 Fragments arranged by size: 1753 1486 958 919 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3355 3355 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2791 2892 3264 4751 1239 Size: 1552 101 372 1487 2579 Fragments arranged by size: 2579 1552 1487 372 101 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2394 2903 3329 4216 5081 5379 2394 Size: 509 426 887 865 298 3106 Fragments arranged by size: 3106 887 865 509 426 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3183 4754 882 Size: 617 1684 1571 2219 Fragments arranged by size: 2219 1684 1571 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 3898 4280 4915 Size: 53 83 186 936 512 1853 382 635 Cuts at: 4915 275 Size: 1451 Fragments arranged by size: 1853 1451 936 635 512 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2985 4524 4922 5196 5343 5754 2985 Size: 1539 398 274 147 411 3322 Fragments arranged by size: 3322 1539 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2030 2060 2789 2819 2967 Size: 30 165 30 627 30 729 30 148 Cuts at: 2967 2997 1178 Size: 30 4272 Fragments arranged by size: 4272 729 627 165 148 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 1186 Size: 961 5130 BseYI C'CCAG_C Cuts at: 5294 5790 5294 Size: 496 5595 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2636 1163 Size: 573 900 4618 Fragments arranged by size: 4618 900 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2502 2612 3195 4152 4237 5308 2502 Size: 110 583 957 85 1071 3285 Fragments arranged by size: 3285 1071 957 583 110 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 BsmBI CGTCTCn'nnnn_ Cuts at: 2052 2052 Size: 6091 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2502 2612 2897 3115 3195 3410 4152 Size: 399 712 110 285 218 80 215 742 Cuts at: 4152 4237 5308 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 712 399 285 218 215 110 85 80 BspEI T'CCGG_A Cuts at: 2985 5754 2985 Size: 2769 3322 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3446 844 Size: 2602 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2071 4586 4760 65 Size: 1183 823 2515 174 1396 Fragments arranged by size: 2515 1396 1183 823 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 2274 4151 5163 2274 Size: 1877 1012 3202 Fragments arranged by size: 3202 1877 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 3093 4895 3093 Size: 1802 4289 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstYI r'GATC_y Cuts at: 1972 2270 3421 3788 4173 4190 5642 5728 5740 Size: 298 1151 367 385 17 1452 86 12 Cuts at: 5740 6086 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1151 385 367 346 298 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5539 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 3607 4401 4421 3607 Size: 794 20 5277 Fragments arranged by size: 5277 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 2349 3395 2349 Size: 1046 5045 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3538 4427 6056 Size: 54 1425 50 275 18 1708 889 1629 Cuts at: 6056 8 Size: 43 Fragments arranged by size: 1708 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4021 1353 Size: 2668 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2260 4678 5052 5198 387 Size: 1064 138 671 2418 374 146 1280 Fragments arranged by size: 2418 1280 1064 671 374 146 138 EcoICRI GAG'CTC Cuts at: 2610 2610 Size: 6091 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2791 2892 3264 3363 4154 4751 5538 Size: 393 1159 101 372 99 791 597 787 Cuts at: 5538 5806 1239 Size: 268 1524 Fragments arranged by size: 1524 1159 791 787 597 393 372 268 101 99 EcoO109I rG'GnC_Cy Cuts at: 2884 2893 2894 2884 Size: 9 1 6081 Fragments arranged by size: 6081 9 1 EcoRI G'AATT_C Cuts at: 2198 2752 2959 3400 3778 2198 Size: 554 207 441 378 4511 Fragments arranged by size: 4511 554 441 378 207 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 5238 1678 Size: 3560 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2699 2731 3062 Size: 32 135 32 458 32 637 32 331 Cuts at: 3062 3094 4732 4764 4806 4838 1373 Size: 32 1638 32 42 32 2626 Fragments arranged by size: 2626 1638 637 458 331 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2288 2797 3435 3681 669 Size: 988 281 350 509 638 246 3079 Fragments arranged by size: 3079 988 638 509 350 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2165 2808 5205 5389 1305 Size: 860 643 2397 184 2007 Fragments arranged by size: 2397 2007 860 643 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4269 Fragments arranged by size: 4269 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2928 4049 4408 4567 518 Size: 648 1762 1121 359 159 2042 Fragments arranged by size: 2042 1762 1121 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2441 3507 4184 5332 5577 1606 Size: 405 430 1066 677 1148 245 2120 Fragments arranged by size: 2120 1148 1066 677 430 405 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5539 NdeI CA'TA_TG Cuts at: 387 2404 387 Size: 2017 4074 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2184 2927 2933 3449 4994 1132 Size: 97 955 743 6 516 1545 2229 Fragments arranged by size: 2229 1545 955 743 516 97 6 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2884 2884 Size: 6091 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PspOMI G'GGCC_C Cuts at: 2893 2893 Size: 6091 PstI C_TGCA'G Cuts at: 830 2630 3331 3443 830 Size: 1800 701 112 3478 Fragments arranged by size: 3478 1800 701 112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2011 2441 2011 Size: 430 5661 SacI G_AGCT'C Cuts at: 2612 2612 Size: 6091 SalI G'TCGA_C Cuts at: 1936 2286 3433 1936 Size: 350 1147 4594 Fragments arranged by size: 4594 1147 350 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SexAI A'CCwGG_T Cuts at: 2298 2298 Size: 6091 SfcI C'TryA_G Cuts at: 826 1045 2436 2626 3327 3439 4574 5255 5446 Size: 219 1391 190 701 112 1135 681 191 Cuts at: 5446 826 Size: 1471 Fragments arranged by size: 1471 1391 1135 701 681 219 191 190 112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2409 2918 3344 4195 5096 5358 Size: 197 926 466 509 426 851 901 262 Cuts at: 5358 820 Size: 1553 Fragments arranged by size: 1553 926 901 851 509 466 426 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 2933 3449 1132 Size: 1801 516 3774 Fragments arranged by size: 3774 1801 516 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2889 3530 513 Size: 552 1824 641 3074 Fragments arranged by size: 3074 1824 641 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2724 4118 4135 4288 4473 4918 325 Size: 1255 1144 1394 17 153 185 445 1498 Fragments arranged by size: 1498 1394 1255 1144 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2445 4337 6049 Size: 274 80 33 51 1443 468 1892 1712 Cuts at: 6049 96 Size: 138 Fragments arranged by size: 1892 1712 1443 468 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2967 2999 6051 6083 2967 Size: 32 3052 32 2975 Fragments arranged by size: 3052 2975 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2065 2065 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 2202 2756 3233 4220 2202 Size: 554 477 987 4073 Fragments arranged by size: 4073 987 554 477 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI