Restriction map for
pFN21AB9631
(Circular) (Six-base) MAPSORT of: pFN21AB9631.seq Check: 2982 from: 1 to: 5521
pFN21AB9631 5521 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2344 2864 1937
Size: 407 520 4594
Fragments arranged by size:
4594 520 407
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2369 3584 4968 5236 1632
Size: 737 1215 1384 268 1917
Fragments arranged by size:
1917 1384 1215 737 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 2049 4420 5397 1203
Size: 846 2371 977 1327
Fragments arranged by size:
2371 1327 977 846
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2802 1184
Size: 1618 3903
AlwNI CAG_nnn'CTG
Cuts at: 3264 4836 3264
Size: 1572 3949
ApaLI G'TGCA_C
Cuts at: 3578 4734 3578
Size: 1156 4365
ApoI r'AATT_y
Cuts at: 1493 2830 3056 3208 5317 1493
Size: 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2277 2295 2432 2584 2846 1870
Size: 73 334 18 137 152 262 4545
Fragments arranged by size:
4545 334 262 152 137 73 18
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2268 2300 2591 2842 4297
Size: 325 217 627 481 32 291 251 1455
Cuts at: 4297 618
Size: 1842
Fragments arranged by size:
1842 1455 627 481 325 291 251 217
32
BanII G_rGCy'C
Cuts at: 1391 2585 2840 1391
Size: 1194 255 4072
Fragments arranged by size:
4072 1194 255
BbeI G_GCGC'C
Cuts at: 2272 2304 2595 2272
Size: 32 291 5198
Fragments arranged by size:
5198 291 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2342 928
Size: 971 443 4107
Fragments arranged by size:
4107 971 443
BbvCI CC'TCA_GC
Cuts at: 2175 2175
Size: 5521
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3712 3746 1903
Size: 34 1775 34 3678
Fragments arranged by size:
3678 1775 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2087 2236 2309
Size: 107 122 71 631 605 415 149 73
Cuts at: 2309 2589 2732 4132 136
Size: 280 143 1400 1525
Fragments arranged by size:
1525 1400 631 605 415 280 149 143
122 107 73 71
BglII A'GATC_T
Cuts at: 5516 5516
Size: 5521
BlpI GC'TnA_GC
Cuts at: 2579 2938 2579
Size: 359 5162
Bme1580I G_kGCm'C
Cuts at: 1790 2494 3582 4738 1790
Size: 704 1088 1156 2573
Fragments arranged by size:
2573 1156 1088 704
BmgBI CAC'GTC
Cuts at: 1318 2050 1318
Size: 732 4789
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3252 4210 442
Size: 416 53 83 399 24 1835 958 1753
Fragments arranged by size:
1835 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4181 1239
Size: 2942 2579
Bpu10I CC'TnA_GC
Cuts at: 2175 2606 2175
Size: 431 5090
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4511 4809 3646
Size: 865 298 4358
Fragments arranged by size:
4358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 2453 2495 4184 882
Size: 617 516 438 42 1689 2219
Fragments arranged by size:
2219 1689 617 516 438 42
BsaAI yAC'GTr
Cuts at: 493 1204 2643 3312 493
Size: 711 1439 669 2702
Fragments arranged by size:
2702 1439 711 669
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2269 2301 2439 2592
Size: 53 83 186 936 736 32 138 153
Cuts at: 2592 3328 3710 4345 275
Size: 736 382 635 1451
Fragments arranged by size:
1451 936 736 736 635 382 186 153
138 83 53 32
BsaWI w'CCGG_w
Cuts at: 3954 4352 4626 4773 5184 3954
Size: 398 274 147 411 4291
Fragments arranged by size:
4291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2781 2811 1178
Size: 30 165 30 1378 30 3888
Fragments arranged by size:
3888 1378 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2144 2147 2665 1186
Size: 955 3 3 518 4042
Fragments arranged by size:
4042 955 518 3 3
BseYI C'CCAG_C
Cuts at: 2242 4724 5220 2242
Size: 2482 496 2543
Fragments arranged by size:
2543 2482 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 2695 1163
Size: 573 606 353 3989
Fragments arranged by size:
3989 606 573 353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2313 2736 2971 3732 4760 1540
Size: 275 186 312 423 235 761 1028 2301
Fragments arranged by size:
2301 1028 761 423 312 275 235 186
BsiHKAI G_wGCw'C
Cuts at: 2421 3582 3667 4738 2421
Size: 1161 85 1071 3204
Fragments arranged by size:
3204 1161 1071 85
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
BsmBI CGTCTCn'nnnn_
Cuts at: 2296 2309 2296
Size: 13 5508
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2421 2494 2585 2840 3582 3667 4738
Size: 399 631 73 91 255 742 85 1071
Cuts at: 4738 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 631 399 255 91 85
73
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2876 844
Size: 2032 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3408 1394
Size: 429 1585 3507
Fragments arranged by size:
3507 1585 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2226 4165 4352 1700
Size: 110 416 1939 187 2869
Fragments arranged by size:
2869 1939 416 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5521
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5521
BssSI C'ACGA_G
Cuts at: 3581 4593 3581
Size: 1012 4509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5521
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstYI r'GATC_y
Cuts at: 1972 2851 3218 3603 3620 5072 5158 5170 5516
Size: 879 367 385 17 1452 86 12 346
Cuts at: 5516 1972
Size: 1977
Fragments arranged by size:
1977 1452 879 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4969
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 2521 2577 3037 3831 3851 2521
Size: 56 460 794 20 4191
Fragments arranged by size:
4191 794 460 56 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2101 4528 809
Size: 507 785 2427 1802
Fragments arranged by size:
2427 1802 785 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2310 2733 2968
Size: 54 1425 50 275 18 480 423 235
Cuts at: 2968 3857 5486 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 480 423 275 235 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2310 2733 2968 1537
Size: 275 498 423 235 4090
Fragments arranged by size:
4090 498 423 275 235
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 2118 2564 3451 1353
Size: 684 81 446 887 3423
Fragments arranged by size:
3423 887 684 446 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2531 4108 4482 4628 387
Size: 1064 138 942 1577 374 146 1280
Fragments arranged by size:
1577 1280 1064 942 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 3584 4181 4968 5236 1239
Size: 393 737 1215 597 787 268 1524
Fragments arranged by size:
1524 1215 787 737 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2542 2542
Size: 5521
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FspI TGC'GCA
Cuts at: 4027 4027
Size: 5521
HaeII r_GCGC'y
Cuts at: 1678 2115 2272 2304 2558 2595 2601 4668 1678
Size: 437 157 32 254 37 6 2067 2531
Fragments arranged by size:
2531 2067 437 254 157 37 32 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2612 2644 4162 4194 4236
Size: 32 135 32 1040 32 1518 32 42
Cuts at: 4236 4268 1373
Size: 32 2626
Fragments arranged by size:
2626 1518 1040 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KasI G'GCGC_C
Cuts at: 2268 2300 2591 2268
Size: 32 291 5198
Fragments arranged by size:
5198 291 32
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3699
Fragments arranged by size:
3699 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2784 3479 3838 3997 518
Size: 648 1618 695 359 159 2042
Fragments arranged by size:
2042 1618 695 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2616 2709 2937 3614 4762 5007 1606
Size: 495 515 93 228 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 515 495 245 228 93
NaeI GCC'GGC
Cuts at: 2228 2228
Size: 5521
NarI GG'CG_CC
Cuts at: 2269 2301 2592 2269
Size: 32 291 5198
Fragments arranged by size:
5198 291 32
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4969
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NgoMIV G'CCGG_C
Cuts at: 2226 2226
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2380 2879 4424 1132
Size: 97 1151 499 1545 2229
Fragments arranged by size:
2229 1545 1151 499 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5521
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PmlI CAC'GTG
Cuts at: 2643 2643
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PstI C_TGCA'G
Cuts at: 830 2873 830
Size: 2043 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
PvuII CAG'CTG
Cuts at: 2709 2709
Size: 5521
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SapI GCTCTTCn'nnn_
Cuts at: 2564 2564
Size: 5521
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SfcI C'TryA_G
Cuts at: 826 1045 2704 2869 4004 4685 4876 826
Size: 219 1659 165 1135 681 191 1471
Fragments arranged by size:
1659 1471 1135 681 219 191 165
SfoI GGC'GCC
Cuts at: 2270 2302 2593 2270
Size: 32 291 5198
Fragments arranged by size:
5198 291 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2279 2586 2848 1872
Size: 407 307 262 4545
Fragments arranged by size:
4545 407 307 262
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3625 4526 4788 820
Size: 197 926 1682 901 262 1553
Fragments arranged by size:
1682 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2380 2879 1132
Size: 1248 499 3774
Fragments arranged by size:
3774 1248 499
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2025 2082 2960 513
Size: 552 960 57 878 3074
Fragments arranged by size:
3074 960 878 552 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3548 3565 3718 3903 4348 325
Size: 1255 1968 17 153 185 445 1498
Fragments arranged by size:
1968 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3767 5479 96
Size: 274 80 33 51 1443 1790 1712 138
Fragments arranged by size:
1790 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2781 2813 5481 5513 2781
Size: 32 2668 32 2789
Fragments arranged by size:
2789 2668 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2277 2584 2846 1870
Size: 407 307 262 4545
Fragments arranged by size:
4545 407 307 262
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI XcmI