(Circular) (Six-base) MAPSORT of: pFN21AB9628.seq Check: 9187 from: 1 to: 5698 pFN21AB9628 5698 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3041 1937 Size: 1104 4594 Acc65I G'GTAC_C Cuts at: 2906 3019 2906 Size: 113 5585 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2169 2445 2472 3761 5145 5413 1632 Size: 537 276 27 1289 1384 268 1917 Fragments arranged by size: 1917 1384 1289 537 276 268 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 2934 4597 5574 1203 Size: 1731 1663 977 1327 Fragments arranged by size: 1731 1663 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 3441 5013 3441 Size: 1572 4126 ApaI G_GGCC'C Cuts at: 2537 2759 2537 Size: 222 5476 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 3023 1870 Size: 73 1080 4545 Fragments arranged by size: 4545 1080 73 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2906 3019 4474 618 Size: 325 217 627 1119 113 1455 1842 Fragments arranged by size: 1842 1455 1119 627 325 217 113 BanII G_rGCy'C Cuts at: 1391 2084 2266 2490 2537 2759 3017 1391 Size: 693 182 224 47 222 258 4072 Fragments arranged by size: 4072 693 258 224 222 182 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2980 928 Size: 971 1081 3646 Fragments arranged by size: 3646 1081 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BfrBI ATG'CAT Cuts at: 2305 2305 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4309 136 Size: 107 122 71 631 605 2637 1525 Fragments arranged by size: 2637 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 2135 3115 2135 Size: 980 4718 Bme1580I G_kGCm'C Cuts at: 1790 2537 2759 3759 4915 1790 Size: 747 222 1000 1156 2573 Fragments arranged by size: 2573 1156 1000 747 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2540 3429 4387 Size: 416 53 83 399 24 1123 889 958 Cuts at: 4387 442 Size: 1753 Fragments arranged by size: 1753 1123 958 889 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 2573 4358 1239 Size: 1214 120 1785 2579 Fragments arranged by size: 2579 1785 1214 120 Bpu10I CC'TnA_GC Cuts at: 2774 2872 2774 Size: 98 5600 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2270 2660 3823 4688 4986 2270 Size: 390 1163 865 298 2982 Fragments arranged by size: 2982 1163 865 390 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2817 4361 882 Size: 617 1318 1544 2219 Fragments arranged by size: 2219 1544 1318 617 BsaAI yAC'GTr Cuts at: 493 1204 3489 493 Size: 711 2285 2702 Fragments arranged by size: 2702 2285 711 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2319 3505 3887 4522 Size: 53 83 186 936 786 1186 382 635 Cuts at: 4522 275 Size: 1451 Fragments arranged by size: 1451 1186 936 786 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4131 4529 4803 4950 5361 4131 Size: 398 274 147 411 4468 Fragments arranged by size: 4468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2031 2061 2462 2492 1178 Size: 30 165 30 628 30 401 30 4384 Fragments arranged by size: 4384 628 401 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5698 BseYI C'CCAG_C Cuts at: 4901 5397 4901 Size: 496 5202 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5125 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3759 3844 4915 3759 Size: 85 1071 4542 Fragments arranged by size: 4542 1071 85 BsmI GAATG_Cn' Cuts at: 2884 3209 3302 2884 Size: 325 93 5280 Fragments arranged by size: 5280 325 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2266 2490 2537 2759 3017 3759 Size: 399 294 182 224 47 222 258 742 Cuts at: 3759 3844 4915 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 294 258 224 222 182 85 47 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3053 844 Size: 2209 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3585 1394 Size: 429 1762 3507 Fragments arranged by size: 3507 1762 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4342 4529 1700 Size: 110 2532 187 2869 Fragments arranged by size: 2869 2532 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 2561 3758 4770 2561 Size: 1197 1012 3489 Fragments arranged by size: 3489 1197 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5698 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2035 Size: 5698 BstYI r'GATC_y Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693 Size: 1056 367 385 17 1452 86 12 346 Cuts at: 5693 1972 Size: 1977 Fragments arranged by size: 1977 1452 1056 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2333 2333 Size: 5698 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4959 Fragments arranged by size: 4959 683 56 BtsI GCAGTG_nn' Cuts at: 3214 4008 4028 3214 Size: 794 20 4884 Fragments arranged by size: 4884 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2397 2440 3145 Size: 54 1425 50 275 18 567 43 705 Cuts at: 3145 4034 5663 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 705 567 275 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2100 2145 2175 2263 3628 1353 Size: 747 45 30 88 1365 3423 Fragments arranged by size: 3423 1365 747 88 45 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4285 4659 4805 387 Size: 1064 138 2696 374 146 1280 Fragments arranged by size: 2696 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2169 2445 2453 2472 2573 3761 4358 Size: 393 537 276 8 19 101 1188 597 Cuts at: 4358 5145 5413 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1188 787 597 537 393 276 268 101 19 8 EcoO109I rG'GnC_Cy Cuts at: 2216 2534 2755 2970 2216 Size: 318 221 215 4944 Fragments arranged by size: 4944 318 221 215 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2264 2264 Size: 5698 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 4845 1678 Size: 3167 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2031 2063 2200 2227 2232 Size: 32 135 32 459 32 137 27 5 Cuts at: 2232 2259 2371 2403 2555 2587 4339 4371 4413 Size: 27 112 32 152 32 1752 32 42 Cuts at: 4413 4445 1373 Size: 32 2626 Fragments arranged by size: 2626 1752 459 152 137 135 112 42 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2050 3042 3288 669 Size: 988 281 112 992 246 3079 Fragments arranged by size: 3079 992 988 281 246 112 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 2910 3023 2910 Size: 113 5585 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2551 4812 4996 1305 Size: 1246 2261 184 2007 Fragments arranged by size: 2261 2007 1246 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2399 2442 10 Size: 54 1425 343 567 43 3266 Fragments arranged by size: 3266 1425 567 343 54 43 MslI CAynn'nnrTG Cuts at: 518 1166 3656 4015 4174 518 Size: 648 2490 359 159 2042 Fragments arranged by size: 2490 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3114 3791 4939 5184 1606 Size: 1508 677 1148 245 2120 Fragments arranged by size: 2120 1508 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5146 NdeI CA'TA_TG Cuts at: 387 2652 387 Size: 2265 3433 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NsiI A_TGCA'T Cuts at: 2307 2307 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 2882 2938 3056 4601 1132 Size: 97 1653 56 118 1545 2229 Fragments arranged by size: 2229 1653 1545 118 97 56 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 2934 4597 2934 Size: 1663 4035 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PfoI T'CCnGG_A Cuts at: 2060 2430 2550 2060 Size: 370 120 5208 Fragments arranged by size: 5208 370 120 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2216 2970 2216 Size: 754 4944 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PspOMI G'GGCC_C Cuts at: 2533 2755 2533 Size: 222 5476 PstI C_TGCA'G Cuts at: 830 2738 3050 830 Size: 1908 312 3478 Fragments arranged by size: 3478 1908 312 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SanDI GG'GwC_CC Cuts at: 2216 2970 2216 Size: 754 4944 SapI GCTCTTCn'nnn_ Cuts at: 2145 2145 Size: 5698 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SexAI A'CCwGG_T Cuts at: 2116 2508 2116 Size: 392 5306 SfcI C'TryA_G Cuts at: 826 1045 2734 2833 2928 3046 4181 4862 5053 Size: 219 1689 99 95 118 1135 681 191 Cuts at: 5053 826 Size: 1471 Fragments arranged by size: 1689 1471 1135 681 219 191 118 99 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 2675 3802 4703 4965 820 Size: 197 926 306 426 1127 901 262 1553 Fragments arranged by size: 1553 1127 926 901 426 306 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2028 3137 513 Size: 552 963 1109 3074 Fragments arranged by size: 3074 1109 963 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3725 3742 3895 4080 4525 325 Size: 1255 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2738 3944 5656 Size: 274 80 33 51 1443 761 1206 1712 Cuts at: 5656 96 Size: 138 Fragments arranged by size: 1712 1443 1206 761 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2462 2494 5658 5690 2462 Size: 32 3164 32 2470 Fragments arranged by size: 3164 2470 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2506 2963 2506 Size: 457 5241 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI