Restriction map for
pFN21AB9627
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BpmI BsaBI BsaI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstF5I BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI HincII
HindII HpaI KpnI KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AhdI 550 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 525 GATCC GGATC(4/5)
Aor51HI 350 AGCGCT AGC^GCT
AspEI 550 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 797 GGACC G^G[AT]CC
AviII 431 TGCGCA TGC^GCA
BbeI 658 GGCGCC GGCGC^C
BlpI 894 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 894 GCTCAGC GC^T[ACGT]AGC
BsaAI 562 TACGTG [CT]AC^GT[GA]
BsgI 565 GTGCAG GTGCAG(16/14)
BspMI 428 ACCTGC ACCTGC(4/8)
BssHII 340 GCGCGC G^CGCGC
BssSI 955 CACGAG CACGAG(-5/-1)
BstAPI 786 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 524 AGATCC [GA]^GATC[CT]
CelII 894 GCTCAGC GC^T[ACGT]AGC
DdeI 895 CTCAG C^T[ACGT]AG
Eam1105I 550 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 350 AGCGCT AGC^GCT
EcoNI 429 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 335 CCAAGG C^C[AT][AT]GG
FspI 431 TGCGCA TGC^GCA
HindIII 358 AAGCTT A^AGCTT
HphI 1095 TCACC GGTGA(8/7)
KasI 658 GGCGCC G^GCGCC
MflI 524 AGATCC [GA]^GATC[CT]
MspA1I 57 CAGCTG C[AC]G^C[GT]G
NarI 658 GGCGCC GG^CGCC
NspI 398 GCATGC [GA]CATG^[CT]
PstI 622 CTGCAG CTGCA^G
PvuII 57 CAGCTG CAG^CTG
SacI 1123 GAGCTC GAGCT^C
SfcI 622 CTGCAG C^T[GA][CT]AG
SfiI 201 GGCCACGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 658 GGCGCC GGC^GCC
SmlI 931 CTCAAG C^T[CT][GA]AG
SphI 398 GCATGC GCATG^C
StyI 335 CCAAGG C^C[AT][AT]GG
TaqI 479 TCGA T^CGA
TfiI 1068 GAATC G^A[AT]TC
TspRI 748 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 479 TCGA T^CGA
XhoII 524 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 658 GGCGCC G[GA]^CG[CT]C
1054 GGCGTC
AlwNI 463 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
568 CAGACTCTG
AspHI 726 GTGCTC G[AT]GC[AT]^C
1123 GAGCTC
BbsI 73 GAAGAC GAAGAC(2/6)
1027 GTCTTC
BpuAI 73 GAAGAC GAAGAC(2/6)
1027 GTCTTC
BsaHI 658 GGCGCC G[GA]^CG[CT]C
1054 GGCGTC
BsiHKAI 726 GTGCTC G[AT]GC[AT]^C
1123 GAGCTC
BsmBI 481 GAGACG CGTCTC(1/5)
823 GAGACG
BsmFI 193 GGGAC GGGAC(10/14)
172 GTCCC
BsrI 276 ACTGG ACTGG(1/-1)
169 CCAGT
BstXI 599 CCACCAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
602 CCAATCTGGTGG
DpnII 477 GATC ^GATC
525 GATC
EagI 672 CGGCCG C^GGCCG
758 CGGCCG
EarI 187 GAAGAG CTCTTC(1/4)
256 GAAGAG
EclXI 672 CGGCCG C^GGCCG
758 CGGCCG
Eco52I 672 CGGCCG C^GGCCG
758 CGGCCG
Hin1I 658 GGCGCC G[GA]^CG[CT]C
1054 GGCGTC
Ksp632I 187 GAAGAG CTCTTC(1/4)
256 GAAGAG
MboI 477 GATC ^GATC
525 GATC
NdeII 477 GATC ^GATC
525 GATC
PleI 570 GACTC GAGTC(4/5)
940 GACTC
Sau3AI 477 GATC ^GATC
525 GATC
SmaI 130 CCCGGG CCC^GGG
155 CCCGGG
XmaCI 130 CCCGGG C^CCGGG
155 CCCGGG
XmaI 130 CCCGGG C^CCGGG
155 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AvaI BanI BanII BbvI BcnI
BfaI BglI BmyI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI MaeI MaeII MboII MnlI
MspI MvaI MvnI MwoI NaeI
NciI NgoMIV NlaIII NlaIV RsaI
Sau96I ScrFI TseI XcmI