(Circular) (Six-base) MAPSORT of: pFN21AB9627.seq Check: 1107 from: 1 to: 5851 pFN21AB9627 5851 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3194 1937 Size: 1257 4594 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3150 3978 4351 1563 Size: 1587 828 373 3063 Fragments arranged by size: 3063 1587 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3914 5298 5566 1632 Size: 2282 1384 268 1917 Fragments arranged by size: 2282 1917 1384 268 AfeI AGC'GCT Cuts at: 2357 2357 Size: 5851 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 4750 5727 1203 Size: 3547 977 1327 Fragments arranged by size: 3547 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 2560 4580 2560 Size: 2020 3831 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2420 2462 1184 Size: 1236 42 4573 Fragments arranged by size: 4573 1236 42 AlwNI CAG_nnn'CTG Cuts at: 2473 2578 3594 5166 2473 Size: 105 1016 1572 3158 Fragments arranged by size: 3158 1572 1016 105 ApaI G_GGCC'C Cuts at: 2322 2897 3116 2322 Size: 575 219 5057 Fragments arranged by size: 5057 575 219 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 3160 3386 3538 5647 1493 Size: 1667 226 152 2109 1697 Fragments arranged by size: 2109 1697 1667 226 152 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 2135 2160 2692 3176 1870 Size: 73 192 25 532 484 4545 Fragments arranged by size: 4545 532 484 192 73 25 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2446 2663 2672 3172 4627 Size: 325 217 627 659 217 9 500 1455 Cuts at: 4627 618 Size: 1842 Fragments arranged by size: 1842 1455 659 627 500 325 217 217 9 BanII G_rGCy'C Cuts at: 1391 2031 2149 2322 2867 2897 3116 3132 3170 Size: 640 118 173 545 30 219 16 38 Cuts at: 3170 1391 Size: 4072 Fragments arranged by size: 4072 640 545 219 173 118 38 30 16 BbeI G_GCGC'C Cuts at: 2667 2667 Size: 5851 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2085 3025 928 Size: 971 186 940 3754 Fragments arranged by size: 3754 971 940 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4042 4076 1903 Size: 34 50 34 2021 34 3678 Fragments arranged by size: 3678 2021 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3743 4953 3743 Size: 1210 4641 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2165 2213 2745 Size: 107 122 71 631 605 493 48 532 Cuts at: 2745 2778 4462 136 Size: 33 1684 1525 Fragments arranged by size: 1684 1525 631 605 532 493 122 107 71 48 33 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 2900 3268 2900 Size: 368 5483 Bme1580I G_kGCm'C Cuts at: 1790 2052 2322 2451 2897 3116 3912 5068 1790 Size: 262 270 129 446 219 796 1156 2573 Fragments arranged by size: 2573 1156 796 446 270 262 219 129 BmgBI CAC'GTC Cuts at: 1318 2047 1318 Size: 729 5122 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3582 4540 442 Size: 416 53 83 399 24 2165 958 1753 Fragments arranged by size: 2165 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4511 1239 Size: 3272 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2921 3976 4841 5139 2921 Size: 1055 865 298 3633 Fragments arranged by size: 3633 1055 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4514 882 Size: 617 3015 2219 Fragments arranged by size: 3015 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2569 3642 493 Size: 711 1365 1073 2702 Fragments arranged by size: 2702 1365 1073 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2664 3060 3658 4040 Size: 53 83 186 936 1131 396 598 382 Cuts at: 4040 4675 275 Size: 635 1451 Fragments arranged by size: 1451 1131 936 635 598 396 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4284 4682 4956 5103 5514 4284 Size: 398 274 147 411 4621 Fragments arranged by size: 4621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2195 2225 2864 2894 1178 Size: 30 165 30 792 30 639 30 4135 Fragments arranged by size: 4135 792 639 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2107 2264 2267 2270 2279 2282 2938 3140 Size: 921 157 3 3 9 3 656 202 Cuts at: 3140 1186 Size: 3897 Fragments arranged by size: 3897 921 656 202 157 9 3 3 3 BseYI C'CCAG_C Cuts at: 2064 2239 5054 5550 2064 Size: 175 2815 496 2365 Fragments arranged by size: 2815 2365 496 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2591 1163 Size: 573 855 4423 Fragments arranged by size: 4423 855 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2680 2766 3109 3301 4062 5090 Size: 275 186 679 86 343 192 761 1028 Cuts at: 5090 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 679 343 275 192 186 86 BsiHKAI G_wGCw'C Cuts at: 2735 3132 3912 3997 5068 2735 Size: 397 780 85 1071 3518 Fragments arranged by size: 3518 1071 780 397 85 BsmI GAATG_Cn' Cuts at: 3362 3455 3362 Size: 93 5758 BsmBI CGTCTCn'nnnn_ Cuts at: 2480 2822 2480 Size: 342 5509 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2052 2149 2322 2451 2735 2867 Size: 399 241 21 97 173 129 284 132 Cuts at: 2867 2897 3116 3132 3170 3912 3997 5068 1391 Size: 30 219 16 38 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 284 241 219 173 132 129 97 85 38 30 21 16 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 3740 5470 3740 Size: 1730 4121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2442 3206 844 Size: 1598 764 3489 Fragments arranged by size: 3489 1598 764 BsrBI CCG'CTC Cuts at: 1394 1823 2302 2899 2911 3738 1394 Size: 429 479 597 12 827 3507 Fragments arranged by size: 3507 827 597 479 429 12 BsrDI GCAATG_nn' Cuts at: 65 1248 4346 4520 65 Size: 1183 3098 174 1396 Fragments arranged by size: 3098 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2618 2675 2765 4495 4682 1700 Size: 110 808 57 90 1730 187 2869 Fragments arranged by size: 2869 1730 808 187 110 90 57 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 2345 1925 Size: 420 5431 BssSI C'ACGA_G Cuts at: 2960 3911 4923 2960 Size: 951 1012 3888 Fragments arranged by size: 3888 1012 951 BstAPI GCAn_nnn'nTGC Cuts at: 794 2797 794 Size: 2003 3848 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5851 BstXI CCAn_nnnn'nTGG Cuts at: 2611 2614 2611 Size: 3 5848 BstYI r'GATC_y Cuts at: 1972 2529 3181 3548 3933 3950 5402 5488 5500 Size: 557 652 367 385 17 1452 86 12 Cuts at: 5500 5846 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 652 557 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2185 2686 2806 3015 3022 513 Size: 552 1120 501 120 209 7 3342 Fragments arranged by size: 3342 1120 552 501 209 120 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5112 Fragments arranged by size: 5112 683 56 BtsI GCAGTG_nn' Cuts at: 3367 4161 4181 3367 Size: 794 20 5037 Fragments arranged by size: 5037 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4858 809 Size: 507 3542 1802 Fragments arranged by size: 3542 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2214 2549 Size: 54 1425 50 275 18 336 48 335 Cuts at: 2549 2616 2677 2763 3298 4187 5816 8 Size: 67 61 86 535 889 1629 43 Fragments arranged by size: 1629 1425 889 535 336 335 275 86 67 61 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2677 2763 3298 1537 Size: 275 865 86 535 4090 Fragments arranged by size: 4090 865 535 275 86 EarI CTCTTCn'nnn_ Cuts at: 1353 2187 2256 3781 1353 Size: 834 69 1525 3423 Fragments arranged by size: 3423 1525 834 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2306 4438 4812 4958 387 Size: 1064 138 717 2132 374 146 1280 Fragments arranged by size: 2132 1280 1064 717 374 146 138 EcoICRI GAG'CTC Cuts at: 3130 3130 Size: 5851 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3914 4511 5298 5566 1239 Size: 393 2282 597 787 268 1524 Fragments arranged by size: 2282 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2438 2438 Size: 5851 EcoO109I rG'GnC_Cy Cuts at: 2893 3112 3113 2893 Size: 219 1 5631 Fragments arranged by size: 5631 219 1 EcoRI G'AATT_C Cuts at: 3160 3538 3160 Size: 378 5473 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 5851 FspI TGC'GCA Cuts at: 2438 4357 2438 Size: 1919 3932 HaeII r_GCGC'y Cuts at: 1678 2359 2522 2667 2999 4998 1678 Size: 681 163 145 332 1999 2531 Fragments arranged by size: 2531 1999 681 332 163 145 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 4492 4524 4566 Size: 32 135 32 974 32 1914 32 42 Cuts at: 4566 4598 1373 Size: 32 2626 Fragments arranged by size: 2626 1914 974 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3195 3441 669 Size: 988 281 1257 246 3079 Fragments arranged by size: 3079 1257 988 281 246 HindIII A'AGCT_T Cuts at: 748 2363 748 Size: 1615 4236 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KasI G'GCGC_C Cuts at: 2663 2663 Size: 5851 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4965 5149 1305 Size: 3660 184 2007 Fragments arranged by size: 3660 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4029 Fragments arranged by size: 4029 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3809 4168 4327 518 Size: 648 2643 359 159 2042 Fragments arranged by size: 2643 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2064 3267 3944 5092 5337 1606 Size: 458 1203 677 1148 245 2120 Fragments arranged by size: 2120 1203 1148 677 458 245 NaeI GCC'GGC Cuts at: 2620 2677 2767 2620 Size: 57 90 5704 Fragments arranged by size: 5704 90 57 NarI GG'CG_CC Cuts at: 2664 2664 Size: 5851 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5299 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NgoMIV G'CCGG_C Cuts at: 2618 2675 2765 2618 Size: 57 90 5704 Fragments arranged by size: 5704 90 57 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 2407 3209 4754 1132 Size: 97 1178 802 1545 2229 Fragments arranged by size: 2229 1545 1178 802 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5851 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5851 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PspOMI G'GGCC_C Cuts at: 2318 2893 3112 2318 Size: 575 219 5057 Fragments arranged by size: 5057 575 219 PstI C_TGCA'G Cuts at: 830 2631 3203 830 Size: 1801 572 3478 Fragments arranged by size: 3478 1801 572 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 PvuII CAG'CTG Cuts at: 2064 2064 Size: 5851 SacI G_AGCT'C Cuts at: 3132 3132 Size: 5851 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SfcI C'TryA_G Cuts at: 826 1045 2627 3199 4334 5015 5206 826 Size: 219 1582 572 1135 681 191 1471 Fragments arranged by size: 1582 1471 1135 681 572 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2213 2213 Size: 5851 SfoI GGC'GCC Cuts at: 2665 2665 Size: 5851 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 2137 2162 3178 1872 Size: 265 25 1016 4545 Fragments arranged by size: 4545 1016 265 25 SmlI C'TyrA_G Cuts at: 820 1017 1943 2936 3955 4856 5118 820 Size: 197 926 993 1019 901 262 1553 Fragments arranged by size: 1553 1019 993 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 2407 3209 1132 Size: 1275 802 3774 Fragments arranged by size: 3774 1275 802 SrfI GCCC'GGGC Cuts at: 2137 2162 2137 Size: 25 5826 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5851 StyI C'CwwG_G Cuts at: 513 1065 2340 3290 513 Size: 552 1275 950 3074 Fragments arranged by size: 3074 1275 950 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3878 3895 4048 4233 4678 325 Size: 1255 2298 17 153 185 445 1498 Fragments arranged by size: 2298 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4097 5809 96 Size: 274 80 33 51 1443 2120 1712 138 Fragments arranged by size: 2120 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5811 5843 5811 Size: 32 5819 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5851 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2347 2608 2611 2347 Size: 261 3 5587 Fragments arranged by size: 5587 261 3 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 2135 2160 3176 1870 Size: 265 25 1016 4545 Fragments arranged by size: 4545 1016 265 25 XmnI GAAnn'nnTTC Cuts at: 3980 3980 Size: 5851 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SwaI