Restriction map for
pFN21AB9625
##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvrII BamHI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
HincII HindII HindIII HpaI HphI
KpnI Ksp632I KspI MaeI MamI
MboI MboII MfeI MflI MluI
MroI MseI MspA1I MunI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 394 GACGTC GACGT^C
AfaI 225 GTAC GT^AC
ApaI 90 GGGCCC GGGCC^C
AspI 54 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 134 CCCGAG C^[CT]CG[GA]G
AviII 293 TGCGCA TGC^GCA
BalI 356 TGGCCA TGG^CCA
BanII 90 GGGCCC G[GA]GC[CT]^C
BbrPI 385 CACGTG CAC^GTG
BmyI 90 GGGCCC G[AGT]GC[ACT]^C
BsaAI 385 CACGTG [CT]AC^GT[GA]
BseRI 190 GAGGAG GAGGAG(10/8)
BsmAI 52 GAGAC GTCTC(1/5)
BsoBI 134 CCCGAG C^[CT]CG[GA]G
Bsp1286I 90 GGGCCC G[AGT]GC[ACT]^C
BstF5I 255 CATCC GGATG(2/0)
BstXI 20 CCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 90 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 20 CCATGG C^C[GA][CT]GG
EcoO109I 90 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 20 CCATGG C^C[AT][AT]GG
FokI 255 CATCC GGATG(9/13)
FspI 293 TGCGCA TGC^GCA
MaeIII 71 GTGAC ^GT[ACGT]AC
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MslI 255 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 20 CCATGG C^CATGG
NlaIII 21 CATG CATG^
PflFI 54 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 384 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 58 GAGTC GAGTC(4/5)
PmaCI 385 CACGTG CAC^GTG
PmlI 385 CACGTG CAC^GTG
PstI 172 CTGCAG CTGCA^G
RsaI 225 GTAC GT^AC
SfcI 172 CTGCAG C^T[GA][CT]AG
StyI 20 CCATGG C^C[AT][AT]GG
TfiI 345 GATTC G^A[AT]TC
Tsp45I 71 GTGAC ^GT[GC]AC
TspRI 69 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 54 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 384 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaII 30 GGACC G^G[AT]CC
115 GGTCC
BbeI 200 GGCGCC GGCGC^C
206 GGCGCC
BspMI 32 ACCTGC ACCTGC(4/8)
170 ACCTGC
BsrFI 197 GCCGGC [GA]^CCGG[CT]
203 GCCGGC
Cfr10I 197 GCCGGC [GA]^CCGG[CT]
203 GCCGGC
EaeI 159 TGGCCG [CT]^GGCC[GA]
356 TGGCCA
FauI 83 GCGGG CCCGC(4/6)
88 GCGGG
HinfI 58 GAGTC G^A[ACGT]TC
345 GATTC
KasI 200 GGCGCC G^GCGCC
206 GGCGCC
MaeII 386 ACGT A^CGT
395 ACGT
NaeI 197 GCCGGC GCC^GGC
203 GCCGGC
NarI 200 GGCGCC GG^CGCC
206 GGCGCC
NgoMIV 197 GCCGGC G^CCGGC
203 GCCGGC
SfoI 200 GGCGCC GGC^GCC
206 GGCGCC
SgrAI 196 CGCCGGCG C[GA]^CCGG[CT]G
202 CGCCGGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
BanI BbvI BcnI BsaHI BsaJI
BsiYI BslI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI MnlI
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI SfaNI TseI