Restriction map for
pFN21AB9625
(Linear) (Six-base) MAPSORT of: KIBB9625.seq Check: 2986 from: 1 to: 399
KIBB9625.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AatII G_ACGT'C
Cuts at: 0 398 399
Size: 398 1
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 399
Size: 51 348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 319 399
Size: 319 80
AlwNI CAG_nnn'CTG
Cuts at: 0 55 74 180 399
Size: 55 19 106 219
Fragments arranged by size:
219 106 55 19
ApaI G_GGCC'C
Cuts at: 0 94 399
Size: 94 305
AvaI C'yCGr_G
Cuts at: 0 134 399
Size: 134 265
BanI G'GyrC_C
Cuts at: 0 100 200 206 399
Size: 100 100 6 193
Fragments arranged by size:
193 100 100 6
BanII G_rGCy'C
Cuts at: 0 94 399
Size: 94 305
BbeI G_GCGC'C
Cuts at: 0 204 210 399
Size: 204 6 189
Fragments arranged by size:
204 189 6
Bme1580I G_kGCm'C
Cuts at: 0 94 399
Size: 94 305
BsaAI yAC'GTr
Cuts at: 0 387 399
Size: 387 12
BsaHI Gr'CG_yC
Cuts at: 0 201 207 395 399
Size: 201 6 188 4
Fragments arranged by size:
201 188 6 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 399
Size: 205 194
Bsp1286I G_dGCh'C
Cuts at: 0 94 399
Size: 94 305
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 179 399
Size: 41 138 220
Fragments arranged by size:
220 138 41
BsrFI r'CCGG_y
Cuts at: 0 197 203 399
Size: 197 6 196
Fragments arranged by size:
197 196 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 399
Size: 27 372
BtgI C'CryG_G
Cuts at: 0 20 399
Size: 20 379
EaeI y'GGCC_r
Cuts at: 0 159 356 399
Size: 159 197 43
Fragments arranged by size:
197 159 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 399
Size: 51 348
EcoO109I rG'GnC_Cy
Cuts at: 0 91 399
Size: 91 308
FspI TGC'GCA
Cuts at: 0 295 399
Size: 295 104
HaeII r_GCGC'y
Cuts at: 0 198 204 210 399
Size: 198 6 6 189
Fragments arranged by size:
198 189 6 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 239 271 399
Size: 43 32 164 32 128
Fragments arranged by size:
164 128 43 32 32
KasI G'GCGC_C
Cuts at: 0 200 206 399
Size: 200 6 193
Fragments arranged by size:
200 193 6
MscI TGG'CCA
Cuts at: 0 358 399
Size: 358 41
MslI CAynn'nnrTG
Cuts at: 0 259 399
Size: 259 140
NaeI GCC'GGC
Cuts at: 0 199 205 399
Size: 199 6 194
Fragments arranged by size:
199 194 6
NarI GG'CG_CC
Cuts at: 0 201 207 399
Size: 201 6 192
Fragments arranged by size:
201 192 6
NcoI C'CATG_G
Cuts at: 0 20 399
Size: 20 379
NgoMIV G'CCGG_C
Cuts at: 0 197 203 399
Size: 197 6 196
Fragments arranged by size:
197 196 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 390 399
Size: 390 9
PfoI T'CCnGG_A
Cuts at: 0 117 399
Size: 117 282
PmlI CAC'GTG
Cuts at: 0 387 399
Size: 387 12
PspOMI G'GGCC_C
Cuts at: 0 90 399
Size: 90 309
PstI C_TGCA'G
Cuts at: 0 176 399
Size: 176 223
SfcI C'TryA_G
Cuts at: 0 172 399
Size: 172 227
SfoI GGC'GCC
Cuts at: 0 202 208 399
Size: 202 6 191
Fragments arranged by size:
202 191 6
SgrAI Cr'CCGG_yG
Cuts at: 0 197 203 399
Size: 197 6 196
Fragments arranged by size:
197 196 6
StyI C'CwwG_G
Cuts at: 0 20 399
Size: 20 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 58 399
Size: 58 341
Tth111I GACn'n_nGTC
Cuts at: 0 57 399
Size: 57 342
ZraI GAC'GTC
Cuts at: 0 396 399
Size: 396 3
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApaI AvaI BanI BanII
BbeI Bme1580I BsaAI BsaHI BseRI Bsp1286I BspMI BsrFI
BstXI BtgI EaeI Eco57MI EcoO109I FspI HaeII Hin4I
KasI MscI MslI NaeI NarI NcoI NgoMIV PflMI
PfoI PmlI PspOMI PstI SfcI SfoI SgrAI StyI
TaqII Tth111I ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MspA1I NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI XbaI XcmI XhoI XmaI
XmnI