Restriction map for
pFN21AB9624
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI Asp700I
Asp718I AspHI AvaI AviII AvrII
BamHI BbeI BbsI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BpuAI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmI BsoBI BspEI
BspHI BspLU11I BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII CfoI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KpnI MaeI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tsp509I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 292 GACGTC GACGT^C
AcyI 292 GACGTC G[GA]^CG[CT]C
AflIII 1005 ACACGT A^C[GA][CT]GT
AhdI 597 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 97 GGGCCC GGGCC^C
AseI 889 ATTAAT AT^TAAT
AsnI 889 ATTAAT AT^TAAT
AspEI 597 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 463 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 764 TGGCCA TGG^CCA
BbrPI 1006 CACGTG CAC^GTG
BbvCI 528 CCTCAGC CCTCAGC(-5/-2)
BglI 962 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 176 AGATCT A^GATCT
BsaAI 1006 CACGTG [CT]AC^GT[GA]
BsaBI 453 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 292 GACGTC G[GA]^CG[CT]C
BsaI 563 GAGACC GGTCTC(1/5)
BsmBI 900 GAGACG CGTCTC(1/5)
Bsp1407I 585 TGTACA T^GTACA
BspDI 922 ATCGAT AT^CGAT
BsrBI 190 CCGCTC CCGCTC(-3/-3)
BsrFI 113 ACCGGC [GA]^CCGG[CT]
BsrGI 585 TGTACA T^GTACA
BstXI 458 CCATCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 113 ACCGGC [GA]^CCGG[CT]
ClaI 922 ATCGAT AT^CGAT
DrdI 652 GACCAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 764 TGGCCA [CT]^GGCC[GA]
Eam1105I 597 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 902 GACGC GACGC(5/10)
Hin1I 292 GACGTC G[GA]^CG[CT]C
HincII 874 GTCAAC GT[CT]^[GA]AC
HindII 874 GTCAAC GT[CT]^[GA]AC
MaeIII 650 GTGAC ^GT[ACGT]AC
MamI 453 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 764 TGGCCA TGG^CCA
MscI 764 TGGCCA TGG^CCA
MseI 890 TTAA T^TAA
NspI 238 ACATGC [GA]CATG^[CT]
PflFI 463 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 426 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 659 GAGTC GAGTC(4/5)
PmaCI 1006 CACGTG CAC^GTG
PmlI 1006 CACGTG CAC^GTG
PpuMI 595 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 889 ATTAAT AT^TAAT
PstI 492 CTGCAG CTGCA^G
PvuII 838 CAGCTG CAG^CTG
SapI 192 GCTCTTC GCTCTTC(1/4)
SexAI 464 ACCTGGT A^CC[AT]GGT
SfcI 492 CTGCAG C^T[GA][CT]AG
SspBI 585 TGTACA T^GTACA
Tru9I 890 TTAA T^TAA
Tsp45I 650 GTGAC ^GT[GC]AC
Tth111I 463 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 426 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 90 GGCACC G^G[CT][GA]CC
806 GGCACC
BanII 97 GGGCCC G[GA]GC[CT]^C
856 GGGCTC
BcnI 5 CCCGG CC^[GC]GG
896 CCCGG
BsgI 844 GTGCAG GTGCAG(16/14)
950 GTGCAG
BspMI 428 ACCTGC ACCTGC(4/8)
342 GCAGGT
BstYI 176 AGATCT [GA]^GATC[CT]
749 GGATCT
DdeI 353 CTGAG C^T[ACGT]AG
529 CTCAG
EcoT14I 654 CCAAGG C^C[AT][AT]GG
758 CCAAGG
HphI 111 TCACC GGTGA(8/7)
860 TCACC
KspI 86 CCGCGG CCGC^GG
282 CCGCGG
MaeII 293 ACGT A^CGT
1007 ACGT
MflI 176 AGATCT [GA]^GATC[CT]
749 GGATCT
MslI 408 CATCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
672 CACCAGCGTG
NciI 5 CCCGG CC^[GC]GG
896 CCCGG
SacII 86 CCGCGG CCGC^GG
282 CCGCGG
StyI 654 CCAAGG C^C[AT][AT]GG
758 CCAAGG
TfiI 307 GATTC G^A[AT]TC
558 GATTC
XcmI 374 CCAACCCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
611 CCAGCGGGGGCCTGG
XhoII 176 AGATCT [GA]^GATC[CT]
749 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I Cfr13I DpnII DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI TspRI TthHB8I