(Circular) (Six-base) MAPSORT of: pFN21AB9619.seq Check: 2216 from: 1 to: 5746 pFN21AB9619 5746 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2953 2953 Size: 5746 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2520 3089 1937 Size: 583 569 4594 Fragments arranged by size: 4594 583 569 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3809 5193 5461 1632 Size: 2177 1384 268 1917 Fragments arranged by size: 2177 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 2472 4645 5622 1203 Size: 1269 2173 977 1327 Fragments arranged by size: 2173 1327 1269 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2846 1184 Size: 1662 4084 AlwNI CAG_nnn'CTG Cuts at: 2579 3019 3489 5061 2579 Size: 440 470 1572 3264 Fragments arranged by size: 3264 1572 470 440 ApaI G_GGCC'C Cuts at: 2074 2499 2511 2074 Size: 425 12 5309 Fragments arranged by size: 5309 425 12 ApaLI G'TGCA_C Cuts at: 2008 3803 4959 2008 Size: 1795 1156 2795 Fragments arranged by size: 2795 1795 1156 ApoI r'AATT_y Cuts at: 1493 3055 3281 3433 5542 1493 Size: 1562 226 152 2109 1697 Fragments arranged by size: 2109 1697 1562 226 152 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2320 2510 2587 3071 1870 Size: 73 377 190 77 484 4545 Fragments arranged by size: 4545 484 377 190 77 73 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2594 2708 3067 4522 618 Size: 325 217 627 807 114 359 1455 1842 Fragments arranged by size: 1842 1455 807 627 359 325 217 114 BanII G_rGCy'C Cuts at: 1391 2074 2170 2225 2499 2511 2624 3065 1391 Size: 683 96 55 274 12 113 441 4072 Fragments arranged by size: 4072 683 441 274 113 96 55 12 BbeI G_GCGC'C Cuts at: 2598 2712 2598 Size: 114 5632 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2802 2912 928 Size: 971 903 110 3762 Fragments arranged by size: 3762 971 903 110 BbvCI CC'TCA_GC Cuts at: 2558 2558 Size: 5746 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 1903 Size: 34 2000 34 3678 Fragments arranged by size: 3678 2000 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2054 3638 4848 2054 Size: 1584 1210 2952 Fragments arranged by size: 2952 1584 1210 BclI T'GATC_A Cuts at: 1548 2298 1548 Size: 750 4996 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2187 4357 136 Size: 107 122 71 631 605 515 2170 1525 Fragments arranged by size: 2170 1525 631 605 515 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 2012 2074 2499 2511 3807 4963 1790 Size: 222 62 425 12 1296 1156 2573 Fragments arranged by size: 2573 1296 1156 425 222 62 12 BmgBI CAC'GTC Cuts at: 1318 2998 1318 Size: 1680 4066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2894 2912 4406 1239 Size: 1655 18 1494 2579 Fragments arranged by size: 2579 1655 1494 18 Bpu10I CC'TnA_GC Cuts at: 2558 2558 Size: 5746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2228 2897 3871 4736 5034 2228 Size: 669 974 865 298 2940 Fragments arranged by size: 2940 974 865 669 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4409 882 Size: 617 2910 2219 Fragments arranged by size: 2910 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2458 3537 493 Size: 711 1254 1079 2702 Fragments arranged by size: 2702 1254 1079 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2309 2385 2517 2595 Size: 53 83 186 936 776 76 132 78 Cuts at: 2595 2709 2763 3553 3935 4570 275 Size: 114 54 790 382 635 1451 Fragments arranged by size: 1451 936 790 776 635 382 186 132 114 83 78 76 54 53 BsaWI w'CCGG_w Cuts at: 2145 4179 4577 4851 4998 5409 2145 Size: 2034 398 274 147 411 2482 Fragments arranged by size: 2482 2034 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5521 Fragments arranged by size: 5521 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2408 2942 1186 Size: 1222 534 3990 Fragments arranged by size: 3990 1222 534 BseYI C'CCAG_C Cuts at: 2504 2572 2692 2970 4949 5445 2504 Size: 68 120 278 1979 496 2805 Fragments arranged by size: 2805 1979 496 278 120 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2385 2426 1163 Size: 573 649 41 4483 Fragments arranged by size: 4483 649 573 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2068 3196 3957 4985 1540 Size: 275 186 67 1128 761 1028 2301 Fragments arranged by size: 2301 1128 1028 761 275 186 67 BsiHKAI G_wGCw'C Cuts at: 2012 2624 3807 3892 4963 2012 Size: 612 1183 85 1071 2795 Fragments arranged by size: 2795 1183 1071 612 85 BsmI GAATG_Cn' Cuts at: 3257 3350 3257 Size: 93 5653 BsmBI CGTCTCn'nnnn_ Cuts at: 2244 2244 Size: 5746 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2074 2170 2225 2499 2511 2624 Size: 399 222 62 96 55 274 12 113 Cuts at: 2624 3065 3807 3892 4963 1391 Size: 441 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 441 399 274 222 113 96 85 62 55 12 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 2988 3635 5365 2988 Size: 647 1730 3369 Fragments arranged by size: 3369 1730 647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2953 3101 844 Size: 2109 148 3489 Fragments arranged by size: 3489 2109 148 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4241 4415 65 Size: 1183 2993 174 1396 Fragments arranged by size: 2993 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2231 2525 2672 2773 2838 2945 4390 Size: 110 421 294 147 101 65 107 1445 Cuts at: 4390 4577 1700 Size: 187 2869 Fragments arranged by size: 2869 1445 421 294 187 147 110 107 101 65 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 2750 2885 1925 Size: 825 135 4786 Fragments arranged by size: 4786 825 135 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 2740 794 Size: 1946 3800 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5746 BstXI CCAn_nnnn'nTGG Cuts at: 2791 2791 Size: 5746 BstYI r'GATC_y Cuts at: 1972 3076 3443 3828 3845 5297 5383 5395 5741 Size: 1104 367 385 17 1452 86 12 346 Cuts at: 5741 1972 Size: 1977 Fragments arranged by size: 1977 1452 1104 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2341 2704 513 Size: 552 1276 363 3555 Fragments arranged by size: 3555 1276 552 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2866 487 Size: 683 56 1640 3367 Fragments arranged by size: 3367 1640 683 56 BtsI GCAGTG_nn' Cuts at: 3262 4056 4076 3262 Size: 794 20 4932 Fragments arranged by size: 4932 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DraIII CAC_nnn'GTG Cuts at: 2856 2856 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2420 4753 809 Size: 507 1104 2333 1802 Fragments arranged by size: 2333 1802 1104 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2065 2133 2677 Size: 54 1425 50 275 18 235 68 544 Cuts at: 2677 2847 3193 4082 5711 8 Size: 170 346 889 1629 43 Fragments arranged by size: 1629 1425 889 544 346 275 235 170 68 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2065 3193 1537 Size: 275 253 1128 4090 Fragments arranged by size: 4090 1128 275 253 EarI CTCTTCn'nnn_ Cuts at: 1353 2260 3676 1353 Size: 907 1416 3423 Fragments arranged by size: 3423 1416 907 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2622 2622 Size: 5746 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2894 2912 3809 4406 5193 5461 1239 Size: 393 1262 18 897 597 787 268 1524 Fragments arranged by size: 1524 1262 897 787 597 393 268 18 EcoO109I rG'GnC_Cy Cuts at: 2071 2495 2496 2071 Size: 424 1 5321 Fragments arranged by size: 5321 424 1 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 2162 2487 2598 2712 4893 1678 Size: 484 325 111 114 2181 2531 Fragments arranged by size: 2531 2181 484 325 114 111 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4387 4419 4461 4493 1373 Size: 32 135 32 2815 32 42 32 2626 Fragments arranged by size: 2815 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2128 3090 3336 669 Size: 988 281 190 962 246 3079 Fragments arranged by size: 3079 988 962 281 246 190 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KasI G'GCGC_C Cuts at: 2594 2708 2594 Size: 114 5632 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4860 5044 1305 Size: 3555 184 2007 Fragments arranged by size: 3555 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2679 10 Size: 54 1425 343 847 3077 Fragments arranged by size: 3077 1425 847 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2015 2789 2813 3704 4063 4222 518 Size: 648 849 774 24 891 359 159 2042 Fragments arranged by size: 2042 891 849 774 648 359 159 24 MspA1I CmG'CkG Cuts at: 1606 2343 2355 2692 2706 3016 3162 3839 4987 Size: 737 12 337 14 310 146 677 1148 Cuts at: 4987 5232 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 737 677 337 310 245 146 14 12 NaeI GCC'GGC Cuts at: 2527 2947 2527 Size: 420 5326 NarI GG'CG_CC Cuts at: 2595 2709 2595 Size: 114 5632 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5194 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NgoMIV G'CCGG_C Cuts at: 2525 2945 2525 Size: 420 5326 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 3104 4649 1132 Size: 97 1875 1545 2229 Fragments arranged by size: 2229 1875 1545 97 PasI CC'CwG_GG Cuts at: 1348 2479 1348 Size: 1131 4615 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PfoI T'CCnGG_A Cuts at: 2955 2955 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PmlI CAC'GTG Cuts at: 2458 2458 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2996 1613 Size: 1383 4363 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PspOMI G'GGCC_C Cuts at: 2070 2495 2507 2070 Size: 425 12 5309 Fragments arranged by size: 5309 425 12 PstI C_TGCA'G Cuts at: 830 3098 830 Size: 2268 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2692 3016 2692 Size: 324 5422 SacI G_AGCT'C Cuts at: 2624 2624 Size: 5746 SacII CC_GC'GG Cuts at: 2344 2707 2344 Size: 363 5383 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 2330 3994 5706 2330 Size: 1664 1712 2370 Fragments arranged by size: 2370 1712 1664 SfcI C'TryA_G Cuts at: 826 1045 3094 4229 4910 5101 826 Size: 219 2049 1135 681 191 1471 Fragments arranged by size: 2049 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2596 2710 2596 Size: 114 5632 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 2231 2672 2773 1700 Size: 531 441 101 4673 Fragments arranged by size: 4673 531 441 101 SmaI CCC'GGG Cuts at: 1872 2322 2512 3073 1872 Size: 450 190 561 4545 Fragments arranged by size: 4545 561 450 190 SmlI C'TyrA_G Cuts at: 820 1017 1943 2243 2912 3850 4751 5013 820 Size: 197 926 300 669 938 901 262 1553 Fragments arranged by size: 1553 938 926 901 669 300 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 3185 513 Size: 552 2120 3074 Fragments arranged by size: 3074 2120 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3773 3790 3943 4128 4573 325 Size: 1255 2193 17 153 185 445 1498 Fragments arranged by size: 2193 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2328 3992 5704 Size: 274 80 33 51 1443 351 1664 1712 Cuts at: 5704 96 Size: 138 Fragments arranged by size: 1712 1664 1443 351 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5706 5738 5706 Size: 32 5714 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 2320 2510 3071 1870 Size: 450 190 561 4545 Fragments arranged by size: 4545 561 450 190 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI