(Circular) (Six-base) MAPSORT of: pFN21AB9616.seq Check: 573 from: 1 to: 5374 pFN21AB9616 5374 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2717 1937 Size: 780 4594 Acc65I G'GTAC_C Cuts at: 2695 2695 Size: 5374 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3437 4821 5089 1632 Size: 1805 1384 268 1917 Fragments arranged by size: 1917 1805 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 4273 5250 1203 Size: 3070 977 1327 Fragments arranged by size: 3070 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5374 AlwNI CAG_nnn'CTG Cuts at: 3117 4689 3117 Size: 1572 3802 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2683 2909 3061 5170 1493 Size: 1190 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2133 2168 2174 2226 2413 2699 1870 Size: 73 190 35 6 52 187 286 4545 Fragments arranged by size: 4545 286 190 187 73 52 35 6 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2305 2510 2590 2695 4150 Size: 325 217 627 518 205 80 105 1455 Cuts at: 4150 618 Size: 1842 Fragments arranged by size: 1842 1455 627 518 325 217 205 105 80 BanII G_rGCy'C Cuts at: 1391 2142 2175 2693 1391 Size: 751 33 518 4072 Fragments arranged by size: 4072 751 518 33 BbeI G_GCGC'C Cuts at: 2309 2514 2594 2309 Size: 205 80 5089 Fragments arranged by size: 5089 205 80 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4403 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3565 3599 1903 Size: 34 1628 34 3678 Fragments arranged by size: 3678 1628 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2666 3985 136 Size: 107 122 71 631 605 994 1319 1525 Fragments arranged by size: 1525 1319 994 631 605 122 107 71 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2791 2791 Size: 5374 Bme1580I G_kGCm'C Cuts at: 1790 2341 3435 4591 1790 Size: 551 1094 1156 2573 Fragments arranged by size: 2573 1156 1094 551 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3105 4063 442 Size: 416 53 83 399 24 1688 958 1753 Fragments arranged by size: 1753 1688 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2341 2341 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4034 1239 Size: 2795 2579 Bpu10I CC'TnA_GC Cuts at: 2384 2384 Size: 5374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3499 4364 4662 3499 Size: 865 298 4211 Fragments arranged by size: 4211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 4037 882 Size: 617 816 1722 2219 Fragments arranged by size: 2219 1722 816 617 BsaAI yAC'GTr Cuts at: 493 1204 3165 493 Size: 711 1961 2702 Fragments arranged by size: 2702 1961 711 BsaBI GATnn'nnATC Cuts at: 5368 5368 Size: 5374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2306 2511 2591 3181 Size: 53 83 186 936 773 205 80 590 Cuts at: 3181 3563 4198 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 773 635 590 382 205 186 83 80 53 BsaWI w'CCGG_w Cuts at: 2097 2281 3807 4205 4479 4626 5037 2097 Size: 184 1526 398 274 147 411 2434 Fragments arranged by size: 2434 1526 411 398 274 184 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 1178 Size: 30 165 30 1041 30 4078 Fragments arranged by size: 4078 1041 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 2465 1186 Size: 1147 132 4095 Fragments arranged by size: 4095 1147 132 BseYI C'CCAG_C Cuts at: 4577 5073 4577 Size: 496 4878 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 2078 2201 1163 Size: 573 297 45 123 4336 Fragments arranged by size: 4336 573 297 123 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2193 2247 2479 2824 3585 4613 Size: 275 186 192 54 232 345 761 1028 Cuts at: 4613 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 345 275 232 192 186 54 BsiHKAI G_wGCw'C Cuts at: 2142 2274 2652 3435 3520 4591 2142 Size: 132 378 783 85 1071 2925 Fragments arranged by size: 2925 1071 783 378 132 85 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2175 2274 2341 2652 2693 3435 Size: 399 352 33 99 67 311 41 742 Cuts at: 3435 3520 4591 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 352 311 99 85 67 41 33 BspEI T'CCGG_A Cuts at: 2281 5037 2281 Size: 2756 2618 BspHI T'CATG_A Cuts at: 2034 3263 4993 2034 Size: 1229 1730 2415 Fragments arranged by size: 2415 1730 1229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 844 Size: 1885 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3261 1394 Size: 429 1438 3507 Fragments arranged by size: 3507 1438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3869 4043 65 Size: 1183 2621 174 1396 Fragments arranged by size: 2621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2188 4018 4205 1700 Size: 110 378 1830 187 2869 Fragments arranged by size: 2869 1830 378 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 2651 3434 4446 2651 Size: 783 1012 3579 Fragments arranged by size: 3579 1012 783 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5374 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5374 BstYI r'GATC_y Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369 Size: 732 367 385 17 1452 86 12 346 Cuts at: 5369 1972 Size: 1977 Fragments arranged by size: 1977 1452 732 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2241 2574 2661 513 Size: 552 939 237 333 87 3226 Fragments arranged by size: 3226 939 552 333 237 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4635 Fragments arranged by size: 4635 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3684 3704 2890 Size: 794 20 4560 Fragments arranged by size: 4560 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 2190 2244 Size: 54 1425 50 275 18 321 39 54 Cuts at: 2244 2821 3710 5339 8 Size: 577 889 1629 43 Fragments arranged by size: 1629 1425 889 577 321 275 54 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2190 2244 2821 1537 Size: 275 378 54 577 4090 Fragments arranged by size: 4090 577 378 275 54 EarI CTCTTCn'nnn_ Cuts at: 1353 2442 3304 1353 Size: 1089 862 3423 Fragments arranged by size: 3423 1089 862 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2317 2645 3961 4335 4481 387 Size: 1064 138 728 328 1316 374 146 1280 Fragments arranged by size: 1316 1280 1064 728 374 328 146 138 EcoICRI GAG'CTC Cuts at: 2140 2140 Size: 5374 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3437 4034 4821 5089 1239 Size: 393 1805 597 787 268 1524 Fragments arranged by size: 1805 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2164 2164 Size: 5374 EcoRI G'AATT_C Cuts at: 2683 3061 2683 Size: 378 4996 FspI TGC'GCA Cuts at: 2126 3880 2126 Size: 1754 3620 HaeII r_GCGC'y Cuts at: 1678 2133 2309 2514 2548 2594 4521 1678 Size: 455 176 205 34 46 1927 2531 Fragments arranged by size: 2531 1927 455 205 176 46 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 4015 4047 4089 Size: 32 135 32 872 32 1539 32 42 Cuts at: 4089 4121 1373 Size: 32 2626 Fragments arranged by size: 2626 1539 872 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2718 2964 669 Size: 988 281 780 246 3079 Fragments arranged by size: 3079 988 780 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KasI G'GCGC_C Cuts at: 2305 2510 2590 2305 Size: 205 80 5089 Fragments arranged by size: 5089 205 80 KpnI G_GTAC'C Cuts at: 2699 2699 Size: 5374 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2609 4488 4672 1305 Size: 1304 1879 184 2007 Fragments arranged by size: 2007 1879 1304 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3552 Fragments arranged by size: 3552 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3332 3691 3850 518 Size: 648 843 1323 359 159 2042 Fragments arranged by size: 2042 1323 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2110 2576 2790 3467 4615 4860 1606 Size: 504 466 214 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 504 466 245 214 NaeI GCC'GGC Cuts at: 2190 2190 Size: 5374 NarI GG'CG_CC Cuts at: 2306 2511 2591 2306 Size: 205 80 5089 Fragments arranged by size: 5089 205 80 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3883 Fragments arranged by size: 3883 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NgoMIV G'CCGG_C Cuts at: 2188 2188 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2074 2641 2732 4277 1132 Size: 97 845 567 91 1545 2229 Fragments arranged by size: 2229 1545 845 567 97 91 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5374 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5374 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5374 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PspXI vC'TCGA_Gb Cuts at: 2168 2168 Size: 5374 PstI C_TGCA'G Cuts at: 830 2109 2487 2541 2570 2726 830 Size: 1279 378 54 29 156 3478 Fragments arranged by size: 3478 1279 378 156 54 29 PvuI CG_AT'CG Cuts at: 2001 2479 2001 Size: 478 4896 PvuII CAG'CTG Cuts at: 2110 2110 Size: 5374 SacI G_AGCT'C Cuts at: 2142 2142 Size: 5374 SacII CC_GC'GG Cuts at: 2577 2577 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SbfI CC_TGCA'GG Cuts at: 2726 2726 Size: 5374 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2105 2483 2537 2566 2722 3857 4538 Size: 219 1060 378 54 29 156 1135 681 Cuts at: 4538 4729 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1060 681 378 219 191 156 54 29 SfiI GGCCn_nnn'nGGCC Cuts at: 2666 2666 Size: 5374 SfoI GGC'GCC Cuts at: 2307 2512 2592 2307 Size: 205 80 5089 Fragments arranged by size: 5089 205 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2135 2701 1872 Size: 263 566 4545 Fragments arranged by size: 4545 566 263 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 3478 4379 4641 820 Size: 197 926 225 1310 901 262 1553 Fragments arranged by size: 1553 1310 926 901 262 225 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2074 2641 2732 1132 Size: 942 567 91 3774 Fragments arranged by size: 3774 942 567 91 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5374 StyI C'CwwG_G Cuts at: 513 1065 2004 2154 2813 513 Size: 552 939 150 659 3074 Fragments arranged by size: 3074 939 659 552 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3401 3418 3571 3756 4201 325 Size: 1255 1821 17 153 185 445 1498 Fragments arranged by size: 1821 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3620 5332 96 Size: 274 80 33 51 1443 1643 1712 138 Fragments arranged by size: 1712 1643 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5334 5366 5334 Size: 32 5342 Tth111I GACn'n_nGTC Cuts at: 1316 2041 1316 Size: 725 4649 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 2168 1943 Size: 225 5149 XmaI C'CCGG_G Cuts at: 1870 2133 2699 1870 Size: 263 566 4545 Fragments arranged by size: 4545 566 263 XmnI GAAnn'nnTTC Cuts at: 3503 3503 Size: 5374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SanDI SapI SexAI SrfI SwaI XcmI